Abstract:
:Successful T cell activation via the T cell receptor (TCR)/CD3 complex requires at least one contact-dependent second signal delivered by costimulatory molecules, including the B7/BB1 molecule, that are present on antigen-presenting cells (APC). SLE is characterized by multiple complex lymphocyte abnormalities of undefined molecular origin. It is currently unclear whether an intrinsic defect of T cell or an underlying APC dysfunction is responsible for defective in vitro proliferation of T cells from patients with SLE. We planned the present experiments to ask whether the TCR/CD3-mediated and B7/BB1-costimulated T cell proliferation is normal in these patients. We used enriched T cell populations that were stimulated with an anti-CD3 MoAb in the presence of controlled quantities of functional B7/BB1 antigen. Freshly isolated T cells from 17 SLE patients (10 and seven patients with either active or inactive disease, respectively) and 11 normal individuals were cocultured with irradiated B7/BB1-transfected P815 cells or parental P815 cells in the presence of OKT3 MoAb at optimal and suboptimal concentrations for 2.5-7 days. Normal or SLE T cells responded similarly to stimulation via anti-CD3, in the absence of B7/BB1 antigen. A several-fold increase in T cell proliferation in the presence of B7/BB1 antigen was observed. Proliferation was inhibited in the presence of anti-B7/BB1 MoAb, but not with control MoAbs. Interestingly, dose-response curves and time kinetics of B7/BB1 costimulation were similar in T cells from patients with either active or inactive SLE at the time of study, and normal individuals. In addition, no differences in the IL-2 receptor release by T cells cultured under these conditions were observed between SLE patients and normal individuals. These results demonstrate that CD28 signalling is not intrinsically impaired in patients with SLE; further studies to investigate whether abnormal B7/BB1 expression is involved in the autoimmune process are needed.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Sfikakis PP,Oglesby R,Sfikakis P,Tsokos GCdoi
10.1111/j.1365-2249.1994.tb06221.xsubject
Has Abstractpub_date
1994-04-01 00:00:00pages
8-14issue
1eissn
0009-9104issn
1365-2249journal_volume
96pub_type
杂志文章abstract::Using an experimental contact sensitivity model in guinea-pigs, evidence is presented that hapten (DNCB or oxazolone) specific T lymphocytes may persist for several months in previous sites of inflammation. Immunological memory, revealed by accelerated contact skin reactions upon retesting with the hapten, was limited...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1983-01-01 00:00:00
abstract::A phenotypic and functional analysis of the circulating natural killer (NK) cell population was carried out in a series of patients with hairy cell leukaemia (HCL). The overall mean NK activity of both the mononuclear and T cell fractions was reduced compared to that of normal controls (466 lytic units (lu) v 573 lu a...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1986-05-01 00:00:00
abstract::We have examined the cell-mediated immunity (CMI) to Staphylococcus aureus (S. aureus) and Staphylococcal enterotoxin B (SEB) in peripheral blood mononuclear cells (PBMC) from children with atopic dermatitis (AD) and from non-atopic child controls by measurement of proliferative responses and production of the cytokin...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.1997.278-ce1172.x
更新日期:1997-02-01 00:00:00
abstract::Paneth cells are important contributors to the intestinal antimicrobial barrier through synthesis and release of antimicrobial peptides and proteins. Animal studies indicate that Paneth cell numbers, location and granule morphology are altered by infection and zinc status. We examined human tissue to determine whether...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.2004.02374.x
更新日期:2004-02-01 00:00:00
abstract::Studies were performed on eleven patients with inflammatory bowel disease to determine if there was an alteration in concanavalin A (Con A) induced suppressor cell activity. Similar investigations were also performed on twenty-one control subjects and five patients with other inflammatory conditions. Supressor cells w...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1978-06-01 00:00:00
abstract::Peripheral blood mononuclear cells (PBMC) of normal human donors are spontaneously cytotoxic for certain tumour-derived and virus-infected target cells. This so-called natural killing (NK) can be augmented by the action of interferons (IFN) and by IFN-inducers. In this study, we have compared both endogenous and augme...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1982-07-01 00:00:00
abstract::This study was performed to determine whether or not IL-18, formerly called IFN-gamma-inducing factor, is involved in the pathogeneses of allergic disorders. Peripheral blood mononuclear cells (PBMC) were obtained from patients with allergic bronchial asthma (BA), patients with atopic dermatitis (AD) and controls who ...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.2001.01664.x
更新日期:2001-11-01 00:00:00
abstract::Nerve growth factor (NGF) regulates B cell activation and differentiation and is an autocrine survival factor for memory B lymphocytes. We have reported recently that the number of memory B cells is reduced during HIV-1 infection. In this study we evaluated whether alteration in the NGF supply was involved in memory B...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.2003.02145.x
更新日期:2003-05-01 00:00:00
abstract::Complement receptor 1-related gene/protein y (Crry) is a potent murine complement regulator that inhibits C3 convertases. Transgenic mice that overexpress soluble Crry (sCrry), directed systemically by the metallothionein-I promoter, have been used as an animal model for chronic blockade of complement activation. Rece...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.2004.02450.x
更新日期:2004-05-01 00:00:00
abstract::Neutrophil phagocytosis and killing of Candida albicans were examined using a radiometric assay in 25 patients with insulin-dependent diabetes and 17 controls under various in vitro metabolic conditions. Glucose was present at 5, 10 and 50 mM, beta-hydroxybutyrate at 1, 5 and 20 mM and glucose with beta-hydroxybutyrat...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1986-02-01 00:00:00
abstract::Biopsy specimens from patients with treated or untreated polymyositis and muscular dystrophy controls were examined by indirect immunoperoxidase staining with a panel of monoclonal antibodies to human leucocyte antigens. In untreated polymyositis, helper/inducer T cells were the predominant T cell subset. In treated c...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1983-11-01 00:00:00
abstract::Our aims were to identify the differential expression of microRNA (miR)-155, as well as to explore the possible regulatory effects of miR-155 on the differentiation and function of T helper type 17 (Th17) cells in atopic dermatitis (AD). The Th17 cell percentage and expression levels of miR-155, retinoic acid-related ...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/cei.12624
更新日期:2015-07-01 00:00:00
abstract::The proliferative responses to stimulation with phytohaemagglutinin (PHA) in cultures of lymphocytes from newly calved and non-pregnant cattle were compared. Lymphocyte cultures from newly calved cows were seen to respond less to PHA than cultures from the non-pregnant cows. It is suggested that this depression in res...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1977-07-01 00:00:00
abstract::Superantigens (SAgs) are potent stimulators of T cells bearing specific Vbeta T cell receptors (TCR) and may play a role in the pathogenesis of Kawasaki syndrome (KS), although despite 15 years of intense study this area remains controversial. Because SAgs can cause Vbeta restricted T cell activation in the absence of...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章,多中心研究
doi:10.1111/j.1365-2249.2007.03567.x
更新日期:2008-02-01 00:00:00
abstract::In order to delineate the molecular pathogenesis of the increased susceptibility to CMV disease in HIV infection, the patterns of antigen responsiveness in HIV-infected and non-infected individuals were investigated. CMV was fractionated by SDS-PAGE and electroblotted onto nitrocellulose. Lymphoproliferative responses...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.1990.tb05490.x
更新日期:1990-12-01 00:00:00
abstract::The allergenic properties of the proteins of two lyophilized fractions of fresh natural rubber latex obtained by ultracentrifugation, the C serum and the sedimented bottom or lutoid fraction, have been compared with those of the serum proteins of two samples of high ammonia latex (HAL) [A]HALS obtained from HAL stored...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.1996.24731.x
更新日期:1996-05-01 00:00:00
abstract::Previous studies using in vitro systems with various stimuli have shown that PBMC from patients with AD show increased levels of IL-4 but decreased levels of interferon-gamma (IFN-gamma) compared with PBMC from normal controls. However, in vitro conditions do not always mimic the in vivo condition. We therefore believ...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.1997.d01-1015.x
更新日期:1997-05-01 00:00:00
abstract::The relation of clinical and laboratory findings at the time clinical presentation to subsequent survival was investigated in 204 patients with macroglobulinaemia. It was found that the ten following clinical and laboratory parameters were of prognostic significance and a further twelve were not. The best prognoses we...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1977-05-01 00:00:00
abstract::The serum levels of TGF-beta1, measured by solid-phase ELISA, were determined to be significantly augmented in patients with both relapsing remitting (RR) and secondary chronic progressive (CP) MS compared with sex- and age-matched healthy controls. Moreover, in RR MS patients, the blood levels of the cytokine were fu...
journal_title:Clinical and experimental immunology
pub_type: 临床试验,杂志文章
doi:10.1046/j.1365-2249.1998.00604.x
更新日期:1998-07-01 00:00:00
abstract::Dendritic cells were enriched from adenoids of children with otitis media with effusion (OME) by density gradient centrifugation and culture techniques. An enrichment of 40-140-fold was obtained for dendritic cells. These cells were identified using morphology, enzyme cytochemistry, immunocytochemistry and functional ...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.1992.tb03085.x
更新日期:1992-05-01 00:00:00
abstract::Cell-mediated cytotoxicity (CTX) of meningioma patients was assessed postoperatively by a [3H]proline microcytotoxicity test. Autologous and allogeneic tumour cells were used for prelabelling with isotope and peripheral blood lymphocytes added in a ratio of 200:1. After 60 hg the plates were washed and residual CMP co...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1976-05-01 00:00:00
abstract::Serum specimens from 66 HIV-1-infected subjects were tested by ELISA for the presence of IgA antibodies to HIV-1: 44 samples were found positive and 37 were confirmed by immunoblot. In these subjects, the presence of anti-HIV IgA antibodies was studied in relation to the total count of circulating CD4+ lymphocytes and...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.1993.tb05889.x
更新日期:1993-02-01 00:00:00
abstract::Erythrocyte C3b receptors (ECR1) have been measured in 122 pairs of twins (60 monozygotic, 62 dizygotic) using an [125I]-labelled monoclonal antibody (E11). The range of ECR1 numbers was wide, 99-4179 sites/cell, with a log-normal distribution around a geometric mean of 837 sites/cell. The intra-pair variance in dizyg...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1987-10-01 00:00:00
abstract::This report describes the presence of a T lymphocyte subpopulation in germinal centres of lymph follicles. This subpopulation is defined by reactivity with Leu 7 antibody, in addition to OKT11, OKT1, OKT3 and OKT4 positivity. The functional activity of this T lymphocyte subpopulation is a matter of discussion and has ...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1983-12-01 00:00:00
abstract::Cardiomyopathy is the most severe outcome of Chagas disease, causing more than 12 000 deaths/year. Immune cells participate in cardiomyopathy development either by direct tissue destruction, or by driving inflammation. We have shown that CD4- CD8- [double-negative (DN)] T cells are major sources of inflammatory and an...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/cei.12992
更新日期:2017-10-01 00:00:00
abstract::Ageing is a very complex process, the result of the dysregulation of multiple systems interacting in many ways. A prominent change occurring with ageing is related to the architecture and functioning of the immune system, viewed commonly as detrimental and termed 'immunosenescence'. However, age-associated changes may...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章,评审
doi:10.1111/cei.12836
更新日期:2017-01-01 00:00:00
abstract::The present study reports a rare case of systemic M. scrofulaceum infection in an AIDS patient and analyses the inflammatory infiltrate in a lymph node by immunohistochemistry. Special emphasis is put on the histiocytes. The diffuse infiltrate consists mainly of large histiocytes that contain numerous bacilli. These c...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.1991.tb05707.x
更新日期:1991-08-01 00:00:00
abstract::Chronic pulmonary infection is known to constitute a major complication of cystic fibrosis (CF). In order to determine whether a defect of antibody formation is associated with this clinical finding, we have investigated antibody production on the cellular level by using a haemolytic plaque forming cell (PFC) assay. T...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1982-10-01 00:00:00
abstract::Several patients with Paget's disease have been reported to have monoclonal IgM proteins in their serum. We have systematically studied 26 patients with Paget's disease severe enough to require diphosphonate therapy. Five of these patients were found to have isolated elevations of serum IgM which were well outside the...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1984-04-01 00:00:00
abstract::Synovial fluids from rheumatoid arthritis (RA) patients were found to contain activated T lymphocytes that could be maintained as continuous T cell lines (CTCL) in the presence of the T cell growth factor, interleukin (IL)-2. The CTCL predominantly expressed the OKT8 phenotype and were Ia antigen positive. IL-2-depend...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1984-10-01 00:00:00