Nuclear translocation of GAPDH-GFP fusion protein during apoptosis.

Abstract:

:Increased expression and nuclear accumulation of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) are early, critical events in several forms of apoptosis. In order to investigate the subcellular trafficking of GAPDH in vivo, the localization of a GAPDH-green fluorescent protein (GFP) fusion was studied in PC12, HEK 293 and COS-1 cells. In control cells, fusion protein autofluorescence was largely restricted to the cytoplasm, rather than the nuclear concentration favored by GFP alone. In contrast, as early as 30 min after an insult, nuclear fluorescence increased in all cell lines studied. The fusion protein redistribution paralleled the dynamics of endogenous GAPDH. These data suggest that some nuclear GAPDH observed during apoptosis represents protein previously resident in the cytosol. This construct provides an in vivo monitor for an early change in apoptosis.

journal_name

Neuroreport

journal_title

Neuroreport

authors

Shashidharan P,Chalmers-Redman RM,Carlile GW,Rodic V,Gurvich N,Yuen T,Tatton WG,Sealfon SC

doi

10.1097/00001756-199904060-00045

subject

Has Abstract

pub_date

1999-04-06 00:00:00

pages

1149-53

issue

5

eissn

0959-4965

issn

1473-558X

journal_volume

10

pub_type

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