Abstract：
:Electrochemotherapy is an anti-tumour treatment that utilizes locally delivered electric pulses to increase cytotoxicity of chemotherapeutic drugs. The aim of our study was to determine whether anti-tumour effectiveness of electrochemotherapy with cisplatin is a consequence of increased plasma membrane permeability caused by electroporation that enables cisplatin binding to DNA. For this purpose, anti-tumour effectiveness of electrochemotherapy was evaluated on SA-1 tumours treated with electric pulses 3 min after intravenous injection of cisplatin (4 mg kg(-1)). Anti-tumour effectiveness was correlated with platinum accumulation in tumours and the amount of platinum bound to DNA, as determined by atomic absorption spectrometry. In tumours treated with electrochemotherapy, cell kill was increased by a factor of 20 compared with treatment with cisplatin only, as determined from tumour growth curves. The amount of platinum bound to DNA and platinum content in the tumours treated by electrochemotherapy was approximately two times higher than in cisplatin-treated tumours. Based on our results, we conclude that in vivo application of electric pulses potentiates anti-tumour effectiveness of cisplatin by electroporation that consequently results in cisplatin increased delivery into the cells. In addition, besides electroporation, immune system and tumour blood flow changes could be involved in the observed anti-tumour effectiveness of electrochemotherapy.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
Cemazar M,Miklavcic D,Scancar J,Dolzan V,Golouh R,Sersa Gdoi
10.1038/sj.bjc.6690222subject
Has Abstractpub_date
1999-03-01 00:00:00pages
1386-91issue
9-10eissn
0007-0920issn
1532-1827journal_volume
79pub_type
杂志文章abstract：:Patients with recurrent or refractory head and neck squamous cell carcinoma received cisplatin/epinephrine injectable gel or placebo gel injected directly into the clinically dominant tumour. The double-blind phase III trial comprised of up to 6 weekly treatments over 8 weeks, 4 weekly evaluation visits, and then mont...
journal_title：British journal of cancer
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1038/sj.bjc.6600588
更新日期：2002-10-21 00:00:00
abstract：:Primary liver cancer, predominantly consisting of hepatocellular carcinoma (HCC), is one of the most common and aggressive human malignancies worldwide. MicroRNAs (miRNAs) are a class of small non-coding RNAs that regulate gene expression post-transcriptionally. Emerging evidence indicates that miRNAs are often deregu...
journal_title：British journal of cancer
pub_type： 杂志文章,评审
doi：10.1038/sj.bjc.6606010
更新日期：2011-01-18 00:00:00
abstract：:NAD(P)H:quinone oxidoreductase (NQO1, EC 1.6.99.2) is an obligate two-electron reductase that can either bioactivate or detoxify quinones and has been proposed to play an important role in chemoprevention. We have previously characterized a homozygous point mutation in the BE human colon carcinoma cell line that leads...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1997.11
更新日期：1997-01-01 00:00:00
abstract：:In the present study we have used flow cytometric DNA measurements on synchronised human NHIK 3025 cells to measure cell cycle progression under various conditions of reduced oxygenation. Our data indicate that addition of 0.1 mM deoxycytidine or uridine has no effect on the oxygen-dependent arrest in late G1 or on th...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1994.411
更新日期：1994-11-01 00:00:00
abstract：BACKGROUND:In the coBRIM study, cobimetinib plus vemurafenib (C+V) significantly improved survival outcomes vs placebo and vemurafenib (P+V) in patients with advanced/metastatic BRAFV600-mutated melanoma. An analysis of health-related quality of life (HRQOL) from coBRIM is reported. METHODS:Patients completing the Eur...
journal_title：British journal of cancer
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1038/bjc.2017.488
更新日期：2018-03-20 00:00:00
abstract：:Several substances mark the course of lung cancer and may reliably help the clinician in decision-making. This is the first clinical study specifically designed to compare tissue polypeptide antigen and CA 125 tumour associated antigen. Three hundred and eighty-four new lung cancer patients (309 males) were studied at...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6600577
更新日期：2002-11-04 00:00:00
abstract：:The use of cytotoxic drug vincristine (VCR) is limited by the occurrence of peripheral neuropathy. A neurotrophic ACTH(4-9) analogue, ORG 2766, is being studied for its protective effect. Possible modulatory effects of ORG 2766 on tumour cell growth and interference with the cytotoxic efficacy of VCR were studied in t...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1994.90
更新日期：1994-03-01 00:00:00
abstract：:Small cell bronchial carcinoma (SCC) xenografts with differing sensitivity to cyclophosphamide (CY) were investigated using a variety of techniques. Two xenografts (HX78 and HX88) were relatively sensitive to CY, one xenograft (HX72) was inherently resistant to CY and a fourth xenograft (HX78Cy) was a CY induced resis...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1985.98
更新日期：1985-05-01 00:00:00
abstract：:Recently, we have found a high frequency of p53 gene mutations in human functional adrenal tumours. As the tumorigenesis is a multigene defect, we believe that other oncogenes may also be involved in the initiation or progression of adrenal tumours. Using the single-strand conformational polymorphism (SSCP) method, we...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1998.177
更新日期：1998-04-01 00:00:00
abstract：:Two soft-agar methods for assaying chemosensitivity of human cancers in vitro were compared with respect to colony morphology, plating efficiency (PE) and chemosensitivity of human melanomas. In 9 xenografts and 9 patients' biopsy specimens Method A (essentially that of Courtenay & Mills, 1978) gave considerably highe...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1981.223
更新日期：1981-10-01 00:00:00
abstract：:A mini-review of the literature concerning epigenetic gene regulation in cancer. ...
journal_title：British journal of cancer
pub_type： 杂志文章,评审
doi：10.1054/bjoc.2000.1549
更新日期：2000-12-01 00:00:00
abstract：:Multidrug resistance is often associated with the (over)expression of drug efflux transporters of the ATP-binding cassette (ABC) protein family. This minireview discusses the role of one selected ABC-transporter family member, the breast cancer resistance protein (BCRP/ABCG2), in the (pre)clinical efficacy of novel ex...
journal_title：British journal of cancer
pub_type： 杂志文章,评审
doi：10.1038/sj.bjc.6604213
更新日期：2008-03-11 00:00:00
abstract：BACKGROUND:Lithium accumulates in the colon and inhibits the enzyme GSK-3β that possesses anti-carcinogenic effects. We therefore examined the association between lithium use and colorectal cancer risk in a nationwide study. METHODS:We used the Danish Cancer Registry to identify all patients diagnosed with incident co...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2016.10
更新日期：2016-03-01 00:00:00
abstract：:Anti-inflammatory corticosteroids administered to rats in high dosages before intravenous injection of allogeneic tumour cells caused 5-10 fold reductions in "take" and clonogenic growth of the cells in lung and kidney and decreased growth and spread of the cells transplanted to leg muscle. Steroid therapy also reduce...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1974.84
更新日期：1974-05-01 00:00:00
abstract：BACKGROUND:Vascular endothelial growth factor (VEGF)-mediated angiogenesis mediates tumour growth and metastasis. Meningiomas are primarily benign, slow-growing, highly vascularised tumours. Aside from VEGF, there is little data on the function of major angiogenic proteins in meningiomas. METHODS:The VEGFA, platelet-d...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2012.459
更新日期：2012-11-06 00:00:00
abstract：:A multicentric, prospective phase III study was carried out with the aim of testing the so-called 'worst drug rule' hypothesis, which suggests the use of an effective but 'less active' regimen that first eradicates tumoral cells resistant to a second effective and 'more active' regimen. With respect to this hypothesis...
journal_title：British journal of cancer
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：10.1038/bjc.1997.586
更新日期：1997-01-01 00:00:00
abstract：:Sera from breast cancer patients and from female controls were tested for inhibition of lysis of antibody-coated target cells by human leukocytes (K cells). Sera from 39% of breast cancer patients, but from only 8% of controls, inhibited lysis by more than 30%. This inhibition was unrelated to the stage of the disease...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1976.224
更新日期：1976-12-01 00:00:00
abstract：:At baseline, weight-losing pancreatic cancer patients (n=7) had lower leptin (P<0.05) but higher cortisol, interleukin-6, resting energy expenditure and fat oxidation than healthy subjects (n=6, P<0.05). Over a 4 h feeding period, the areas under the curve for glucose, cortisol and interleukin-6 were greater (P<0.05),...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6601712
更新日期：2004-03-22 00:00:00
abstract：:In the BUPA study, a prospective study of 22,000 men attending a screening centre in London, the mean serum cholesterol level of the 267 men who developed cancer was 6.66 mmol l-1, not significantly different from the mean level of 6.72 mmol l-1 among the 525 unaffected controls matched for age, smoking history and th...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1989.198
更新日期：1989-06-01 00:00:00
abstract：:For the first time in a clinical study oral Ifosfamide was used: 65 elderly or unfit patients with small cell lung cancer (SCLC) were treated as outpatients with fractionated oral Ifosfamide and Etoposide. Forty patients (62%) had extensive stage (ED) disease. The median age of the patients was 66 years. In the 60 pat...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1989.265
更新日期：1989-08-01 00:00:00
abstract：:Hypoxic cells in KHT sarcomas were characterized using fluorescence activated cell sorting based on the diffusion properties of the fluorochrome Hoechst 33342. Tumour-bearing female C3H/HeJ mice were injected i.v. with 10 micrograms g-1 Hoechst 33342 and the cells derived from the tumours sorted on the basis of their ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1988.207
更新日期：1988-09-01 00:00:00
abstract：:The abilities of chemokines in orchestrating cellular migration are utilised by different (patho-)biological networks including malignancies. However, except for CXCR4/CXCL12, little is known about the relation between tumour-related chemokine expression and the development and progression of solid tumours like breast...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6604621
更新日期：2008-09-16 00:00:00
abstract：BACKGROUND:Previous epidemiologic studies have shown inconsistent results for the association between alcohol intake and endometrial cancer risk. Most of the studies, however, assessed alcohol intake after cancer diagnosis, or measured alcohol intake at baseline only. METHODS:We prospectively examined the association ...
journal_title：British journal of cancer
pub_type： 杂志文章,多中心研究
doi：10.1038/bjc.2014.257
更新日期：2014-07-08 00:00:00
abstract：:People affected by cancer want information about their prognosis but clinicians have trouble estimating and talking about it. We sought to determine the nature and accuracy of medical oncologists' estimates of life expectancy in newly referred patients with incurable cancer. With reference to each patient, medical onc...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6602908
更新日期：2006-01-30 00:00:00
abstract：:A previous publication (Leith et al., 1992) showed that administration of basic fibroblast growth factor (FGF-2, 0.25 mg kg-1, q.i.d. x 7) to mice bearing xenografted DLD-2 human colon cancers would increase treated tumour growth rates as compared to control neoplasms. Additionally, at the end of the 7 day treatment p...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1993.418
更新日期：1993-10-01 00:00:00
abstract：:A recent study showed an association between a single base substitution, T-->C, in the promotor region of the CYP17 gene, the risk of breast cancer and age at menarche in Asian, African-American and Latino women from California and Hawaii. The C allele was associated with increased risk of breast cancer, significantly...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1998.340
更新日期：1998-06-01 00:00:00
abstract：:Data collected from lung cancer patients attending the Victoria Clinic of the British Columbia Cancer Agency are used to investigate how resources are rationed in the treatment of non-small-cell lung cancer (NSCLC). An ordered logit model is estimated to analyse empirically the relationship between treatment selection...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1996.136
更新日期：1996-03-01 00:00:00
abstract：:Reductions in cell-cell adhesion and stromal and vascular invasion are essential steps in the progression from localized malignancy to metastatic disease. In this study, changes in the expression of the components of the E-cadherin-catenin cell adhesion complex have been investigated using immunohistochemical techniqu...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1997.252
更新日期：1997-01-01 00:00:00
abstract：:Screen-detected (SD) breast cancers are smaller and biologically more indolent than clinically presenting cancers. An often debated question is: if left undiagnosed during their preclinical phase, would they become more aggressive or would they only increase in size? This study considered a registry-based series (1988...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6602289
更新日期：2005-01-17 00:00:00
abstract：:Apoptosis is a physiological form of cell death important in normal processes such as morphogenesis and the functioning of the immune system. In addition, defects in the apoptotic process play a major role in a number of important areas of disease, such as autoimmune diseases and cancer. DNA-damage-induced apoptosis p...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6603257
更新日期：2006-08-21 00:00:00
