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Comparison of two soft-agar methods for assaying chemosensitivity of human tumours in vitro: malignant melanomas.

Abstract：

:Two soft-agar methods for assaying chemosensitivity of human cancers in vitro were compared with respect to colony morphology, plating efficiency (PE) and chemosensitivity of human melanomas. In 9 xenografts and 9 patients' biopsy specimens Method A (essentially that of Courtenay & Mills, 1978) gave considerably higher PE that Method B (essentially that of Hamburger & Salmon, 1977) and, in contrast to Method B, the number of colonies was proportional to the number of cells plated. Evidence was obtained that the observed differences in PE could be attributed to the low O2 concentration and the presence of rat red blood cells in Method A. Colony morphology was similar in the 2 assays. When cells from 4 xenografted melanomas were treated in vitro with DTIC, CCNU, vinblastine and abrin, and the inhibition of colony formation was assayed concurrently in the 2 soft-agar methods, the tumour cells appeared to be more sensitive to 3 of the drugs in Method B than in A. The results demonstrate that chemosensitivity data obtained with the 2 assays cannot be directly compared.

journal_name

Br J Cancer

journal_title

British journal of cancer

authors

Tveit KM,Endresen L,Rugstad HE,Fodstad O,Pihl A

doi

10.1038/bjc.1981.223

subject

Has Abstract

pub_date

1981-10-01 00:00:00

pages

539-44

issue

4

eissn

0007-0920

issn

1532-1827

journal_volume

44

pub_type

杂志文章

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