Abstract:
:The INK4A gene, localized to human chromosome 9p21, encodes p16INK4A, a tumor suppressor that functions at least in part through the inhibition of CDK4, a cyclin-dependent kinase encoded by a gene at 12q13. To examine INK4A gene alterations in uncultured samples of osteosarcoma and the relationship between INK4A and CDK4 alterations, we analyzed the INK4A and CDK4 genes in 87 specimens from 79 patients. INK4A deletion and CDK4 gene amplification were determined by quantitative Southern blot analysis. INK4A exon 2 was screened for mutation by polymerase chain reaction and single-strand conformational polymorphism analysis. Methylation at the CpG island in INK4A, associated with loss of p16INK4A expression, was assessed by Southern blot analysis using methylation-sensitive restriction enzymes. INK4A deletion (4/55) or rearrangement (1/55) was found in 5 of 55 cases. No INK4A exon 2 point mutations and methylation were detected. CDK4 gene amplification was found in 6 of 67 samples, but not in tumors with INK4A alteration. Amplification analysis of other genes at 12q13 (GLI, CHOP, HMGI-C and MDM2) in these 6 cases supports the view that CDK4 and MDM2 are independent targets for amplification, with variable amplification of the intervening region containing HMGI-C. Of 46 patients studied for both INK4A alterations and CDK4 amplification, the tumors in 22% contained one or the other. The prevalence of these alterations, in conjunction with the reported inactivation of RB in up to 80% of cases, suggests that genetic lesions deregulating the G1 to S cell cycle checkpoint may be an almost constant feature in the pathogenesis of osteosarcoma.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Wei G,Lonardo F,Ueda T,Kim T,Huvos AG,Healey JH,Ladanyi Mdoi
10.1002/(sici)1097-0215(19990118)80:2<199::aid-ijcsubject
Has Abstractpub_date
1999-01-18 00:00:00pages
199-204issue
2eissn
0020-7136issn
1097-0215pii
10.1002/(SICI)1097-0215(19990118)80:2<199::AID-IJCjournal_volume
80pub_type
临床试验,杂志文章abstract::A series of 722 lung carcinomas, surgically resected and typed some years ago according to the 1967 WHO classification, was independently reviewed by 2 observers in order to test the reproducibility of histopathological typing when using the criteria of the 1981 WHO classification. Typing was fully agreed upon in 87% ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910530205
更新日期:1993-01-21 00:00:00
abstract::Glioblastoma are notorious for their highly invasive growth, diffusely infiltrating adjacent brain structures that precludes complete resection, and is a major obstacle for cure. To characterize this "invisible" tumor part, we designed a high resolution multimodal imaging approach assessing in vivo the metabolism of i...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.31299
更新日期:2018-07-01 00:00:00
abstract::We have examined, in proliferating neoplastic cells, the effect on DNA of EGTA (a chelator of Ca2+) and W7 (an inhibitor of calmodulin). The treatment results in release of single-stranded DNA fragments (2-10 kb) from pre-labelled HMW DNA. When DNA from synchronized neoplastic cells in S phase is examined, almost all ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910370615
更新日期:1986-06-15 00:00:00
abstract::Transfer of the herpes simplex virus type I thymidine kinase (HSV-TK) gene into tumor cells using virus-based vectors in conjunction with ganciclovir (GCV) exposure provides a potential gene therapy strategy for the treatment of cancer. The possibility of using a novel targetable Sindbis virus expression vector contai...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19990105)80:1<110::aid-ijc
更新日期:1999-01-05 00:00:00
abstract::Head and neck squamous cell carcinoma (HNSCC) is a morbid cancer with poor outcomes. Statins possess anticancer properties such as immunomodulatory and anti-inflammatory effects. The objective of our study is to identify the association between statin use among untreated HNSCC patients and overall death, disease-speci...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.33441
更新日期:2020-12-15 00:00:00
abstract::Epidermal growth factor receptor (EGFR) plays an important role in essential cellular processes such as proliferation, survival and migration. Aberrant activation of EGFR is frequently found in human cancers of various origins and has been implicated in cancer pathogenesis. The therapeutic antibody cetuximab (Erbitux)...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.28585
更新日期:2014-06-01 00:00:00
abstract::Although the combination of cisplatin and etoposide has been used as standard therapy for small-cell lung cancer, it is difficult to demonstrate combination effects between cisplatin and etoposide in vitro. We therefore adopted a 3-dimensional (3-D) model to analyze the combination effects of anticancer drugs, and com...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19970502)71:3<311::aid-ijc
更新日期:1997-05-02 00:00:00
abstract::In STU mice bearing metastasizing SV40-transformed 51A-232B-M tumors, an immune response against a cellular 60kDa protein (p60) developed in about 50% of the tumor-bearing animals, in addition to the response against SV40 large T-antigen and cellular protein p53. The anti-p60 auto-immune response could be observed as ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910420620
更新日期:1988-12-15 00:00:00
abstract::Previous studies have shown that the in vitro-selected adriamycin-resistant human small-cell lung-carcinoma cell line GLC4-ADR150 displays multidrug resistance as the result of 3-fold decreased DNA-topoisomerase II (topo II) activity and a 6-fold reduction in adriamycin accumulation. Not the MDR1 gene, but the MRP gen...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910610317
更新日期:1995-05-04 00:00:00
abstract::Gene transfer is an attractive approach to fight cancer by targeting cancer cells or their vasculature. Our study reports the inhibition of tumor growth and angiogenesis by a nonviral method using dendrimers associated with 36-mer anionic oligomers (ON36) for delivering angiostatin (Kringle 1-3) and tissue inhibitor o...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.11105
更新日期:2003-06-20 00:00:00
abstract::In order to target NK cells against the Hodgkin's-derived cell line L540, we developed bispecific monoclonal antibodies (Bi-MAbs) by somatic hybridization of the 2 mouse hybridoma cell line HRS-3 and A9 which produce monoclonal antibodies (MAbs) with reactivity against the Hodgkin and Reed-Sternberg cell-associated CD...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910550523
更新日期:1993-11-11 00:00:00
abstract::The relative proportions of T, B and T gamma suppressor cells were determined sequentially in peripheral blood of melanoma patients before and after surgery. Concanavalin A (Con A)-induced suppression against lymphocyte mitogenesis of melanoma patients and age matched controls was measured concurrently. The mean perce...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910280103
更新日期:1981-07-15 00:00:00
abstract::LAF-4, which encodes a nuclear protein with transactivation potential, is fused to the MLL gene in acute lymphoblastic leukemia (ALL). We identified LAF-4 as a gene that is transcriptionally deregulated in breast tumors and thus may have a pathological role in mammary tumorigenesis. In line with the previous finding t...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.20881
更新日期:2005-07-01 00:00:00
abstract::The human surface antigen CD24 is over-expressed in small-cell lung cancer. Here we describe the isolation, sequencing and functional characterization of the 5'-flanking region of the human CD24 gene. A sequence (accession number: Y14692) of 3.4 kb regulates the activity of a luciferase reporter gene in CD24-expressin...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19981109)78:4<496::aid-ijc
更新日期:1998-11-09 00:00:00
abstract::Meibomian gland carcinoma (MGC) and basal cell carcinoma (BCC) are common eyelid carcinomas that exhibit highly dissimilar degrees of proliferation and prognoses. We address here the question of the differential mechanisms between these two eyelid cancers that explain their different outcome. A total of 102 confirmed ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29882
更新日期:2016-03-15 00:00:00
abstract::Established cell lines derived from human malignant astrocytomas typically express a combination of platelet-derived growth factor (PDGF) and PDGF receptor which could form an autocrine loop. In this study, we screened for the essential components of a PDGF autocrine loop in fresh surgical isolates of human astrocytom...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910600206
更新日期:1995-01-17 00:00:00
abstract::Patients with head-and-neck cancer commonly have immune defects. It was reported that these patients have raised serum IgA levels. We investigated whether IgA-anti-Fab autoantibodies, which occur in association with immune dysfunction, are present in patients with head-and-neck cancer. Sera of 101 patients with squamo...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910570109
更新日期:1994-04-01 00:00:00
abstract::A new cell line (K-LL-3) with exceptional characteristics was derived from a bone-marrow aspirate from a patient with poorly differentiated lymphoblastic lymphoma. The cell line was established and the cells maintained on agar feeders for over 1 year. The morphology and cytochemical staining of the primary tumor and t...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910230409
更新日期:1979-04-15 00:00:00
abstract::Few studies have examined cancer-related risk factors in relation to SES across the lifecourse in low to middle income countries. This analysis focuses on adult women in India, China, Mexico, Russia and South Africa, and examines the association between individual, parental and lifecourse SES with smoking, alcohol, BM...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.30499
更新日期:2017-02-15 00:00:00
abstract::Low levels of anti-viral activity, mainly interferon alpha/beta (IFN-alpha/beta), are regularly found in lymphoid tissues of BALB/c mice infected with the C3H strain of mammary tumor virus. At the time of tumor development, significant amounts of anti-viral activity were detected in homogenates of spleen and mammary t...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910510620
更新日期:1992-07-30 00:00:00
abstract::Tobacco-related carcinogens cause a variety of DNA damage that is repaired by different enzymatic pathways, suggesting that DNA repair plays an important role in tobacco-induced carcinogenesis. In a large hospital-based case-control study, we investigated DNA repair capacity (DRC) as a biomarker for susceptibility to ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.11346
更新日期:2003-10-20 00:00:00
abstract::The relationship between breast cancer and radiation treatment for cervical cancer was evaluated in an international study of 953 women who subsequently developed breast cancer and 1,806 matched controls. Radiation doses to the breast (average 0.31 Gy) and ovaries (average 32 Gy) were reconstructed for exposed subject...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910440103
更新日期:1989-07-15 00:00:00
abstract::DU-PAN-2 is a high-molecular-weight glycoprotein defined by a murine monoclonal antibody (MAb) against a pancreatic ductal adenocarcinoma cell line. In order to evaluate the usefulness of this antigen as a tumor marker, we determined the serum level of the antigen by competitive inhibition radioimmunoassay in sera fro...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910370509
更新日期:1986-05-15 00:00:00
abstract::Eleven population-based cancer registries tabulated second cancers among 133,411 patients diagnosed with testicular cancer, ovarian cancer or Hodgkin's disease between 1945 and 1984. Overall, 3,157 second cancers were observed, as compared with 2,420 expected at least one year after the first cancer. Survivors of test...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910390506
更新日期:1987-05-15 00:00:00
abstract::Low ionic strength (50 to 100 mM NaCl) and pH 6.0 were found to be optimal conditions for in vitro conversion of Epstein-Barr virus (EBV)-determined nuclear antigen (EBNA)-negative nuclei to EBNA-positive nuclei by addition of the complement-fixing (CF) antigen extracted from Raji cells. In vitro conversion of nuclei ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910220505
更新日期:1978-11-15 00:00:00
abstract::We observed previously that two carbohydrate epitopes, extended type 1 chain Le(a)-Le(a) and Le(b)-Le(a), are expressed strongly in human gastric or colorectal cancer and cell lines derived therefrom, but their expression in human normal colorectal cells is highly limited. A monoclonal antibody, termed GNX-8, was esta...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25131
更新日期:2009-12-21 00:00:00
abstract::Somatic mutations in the TP53 gene are the most frequently observed genetic alterations in human malignancies, including breast cancer, which is one of the leading causes of death among women in Brazil. In our study, we determined the frequency and the pattern of TP53 mutations in malignant breast tumors from 120 pati...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.10567
更新日期:2002-09-01 00:00:00
abstract::Immunotherapy targeting programmed cell death-1 (PD-1) has become a standard pharmacological therapy. Although tumor mutation burden level was reported to depend on the tumor location in nonsmall cell lung cancer (NSCLC), predictive impact of the tumor location on the response to anti-PD-1 therapy is unknown. Two hund...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.33030
更新日期:2020-10-15 00:00:00
abstract::Recent studies suggest a potential role of lipid lowering drugs, fibrates and statins, in anticancer treatment. One candidate for tumor chemoprevention is fenofibrate, which is a potent agonist of peroxisome proliferator activated receptor alpha (PPARalpha). Our results demonstrate elevated expression of PPARalpha in ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.23588
更新日期:2008-09-01 00:00:00
abstract::The open reading frames of the phosphoprotein pp58 (BMRFI) and the deoxyribonuclease (BGLF5) of the Epstein-Barr-virus (EBV) strain M-ABA were cloned in the baculovirus expression vectors pAc373 and pAc360 and expressed in the Spodoptera frugiperda (SF158) insect cells. The recombinant phosphoprotein pp58 expressed in...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910480615
更新日期:1991-07-30 00:00:00