Abstract:
BACKGROUND:Mercury causes in mouse strains of the H-2s haplotype an autoimmune syndrome with antibodies to the nucleolar protein fibrillarin and systemic immune complex (IC) deposits. Other strains, like BALB/C (H-2d), develop only IC deposits, and most strains are resistant. However, mercury activates the murine immune system and causes lymphoproliferation in most strains: H-2s strains are high-responders also in this respect, while the relation between lymphoproliferation and autoimmune manifestations is unclear for other strains. We examined the B cell response to mercury in order to better understand the relation between lymphoproliferation and systemic autoimmunity, using the high-responder H-2s strains (A.SW and SJL), the intermediate responder strain BALB/C (H-2d), and the A. TL (H-2tl) and DBA/2 (H-2d) strains which are resistant to systemic autoimmunity. METHODS:During 4-7 weeks of subcutaneous mercuric chloride injections, the number of B cells and the expression of cell surface activation and proliferation markers was monitored by flow cytometry. The number of cytoplasmic Ig+ splenocytes was determined by direct immunofluorescence technique on slides, and serum Ig isotype levels as well anti-ssDNA and anti-DNP antibodies were determined by ELISA. Serum ANA were monitored weekly by indirect immunofluorescence technique. RESULTS:Mercury-treated A.SW and SJL mice (H-2s) developed an increased expression of the proliferation marker CD71 on B cells, an increased number of B cells in the spleen, and an early, strong, but transient increase in serum Ig concentrations of Th1- as well as Th2-regulated Ig isotypes. Mercury-treated H-2s mice rapidly developed a polyclonal B cell response including the IgM isotype, but also antinucleolar antibodies (ANoA) of the IgG isotype with a clumpy pattern, characteristic for antifibrillarin antibodies. The IgG ANoA response was of a long duration and high titer. The A.TL strain (H-2tl) showed only a slight, restricted B cell activation. The BALB/C strain developed a slight, transient B cell activation dominated by IgG1 and IgE, and antinuclear antibodies (ANA). The DBA/2 strain showed only a minimal B cell response without ANA. CONCLUSION:Mercury induces an early, transient, polyclonal B cell activation linked to the H-2A or H-2K locus in H-2s strains on the A background. This polyclonal response differs from the long-lasting, high-titered IgG autoantibody response to a nucleolar antigen with characteristics of fibrillarin in H-2s strains, which indicates that these responses arise from separate mechanisms. Another group of strains, exemplified by BALB/C (H-2d), responds to mercury with a slight, transient, Th2-dominated B cell response, a restricted antibody specificity targeting the cell nucleus, and systemic IC deposits. Another H-2d strain, DBA, is essentially resistant to mercury, illustrating the importance of non-H-2 genes for regulating the response to mercury.
journal_name
Int Arch Allergy Immunoljournal_title
International archives of allergy and immunologyauthors
Johansson U,Hansson-Georgiadis H,Hultman Pdoi
10.1159/000023959subject
Has Abstractpub_date
1998-08-01 00:00:00pages
295-305issue
4eissn
1018-2438issn
1423-0097pii
iaa16295journal_volume
116pub_type
杂志文章abstract:BACKGROUND:Cry j 1 and Cry j 2 are thought to be the major allergens of Japanese cedar pollen. HLA class II types capable of presenting T-cell epitopes in both allergens and their role in induction of T-cell subsets are not well known. METHODS:CD4+ T (Th)-cell clones (TCCs) specific to either Cry j 1 or Cry j 2 were g...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000024194
更新日期:1999-07-01 00:00:00
abstract:BACKGROUND:Assessing high-sensitivity C-reactive protein (hs-CRP) in relation to allergic endpoints can shed light on both the mechanisms of allergic disease development and early non-communicable disease prevention. However, only a few epidemiological studies so far have investigated the relationship in children and a...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,多中心研究
doi:10.1159/000497320
更新日期:2019-01-01 00:00:00
abstract::A quantitative determination of the inflammatory mediators was performed and correlated with complaints and the measurement of the inflammatory cells in nasal secretions of 18 seasonal allergic rhinitis patients (group 1) outside the pollen season and 40 symptomatic patients (group 2) with seasonal allergic rhinitis d...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236855
更新日期:1995-03-01 00:00:00
abstract:BACKGROUND:Allergen molecule-based diagnosis has been suggested to facilitate the identification of disease-causing allergen sources and the prescription of allergen-specific immunotherapy (AIT). The aim of the current study was to compare allergen molecule-based IgE serology with allergen extract-based skin testing fo...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000477442
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:IgE-mediated hypersensitivity to latex has been recognized as an increasing health problem with far-reaching consequences for patients, regarding both their occupational situation and safety in medical care. Therefore, a correct diagnosis of natural rubber latex (NRL) allergy is essential. The purpose of the...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000049518
更新日期:2001-11-01 00:00:00
abstract:BACKGROUND:High antibiotic prescribing rates for adults with an asthma exacerbation have been reported in developed countries, but few studies have assessed the variation of antibiotic and adjunctive treatment in the routine care of children. OBJECTIVE:We evaluated the trends in health resource utilization for childre...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000507857
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND:Natural rubber latex proteins have been implicated in severe allergy in individuals exposed to latex products, particularly health care workers. Until recently, only crude antigens were available to study the immune response in these patients. In recent years a number of relevant allergens have been purified...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000024279
更新日期:1999-12-01 00:00:00
abstract:BACKGROUND:Wheat is known as the most widely consumed food all over the world. Although many types of wheat allergy have been recognized, their treatment still has a long way to go due to the complex pathogenesis. Oral immunotherapy (OIT) is under investigation for the treatment of wheat allergies. Previous studies hav...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000506945
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND:Cow's milk (CM) allergy is the second most common food allergy developed during infancy in Japan. To identify predictors of persistent CM allergy, we investigated the tolerance acquisition rate based on an oral food challenge in children under 6 years of age, diagnosed with immediate-type CM allergy. METHOD...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000486311
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:Hypersensitivity to contrast media (CMs) may be common and serious. AIM:To evaluate the prevalence of CM hypersensitivity, risk factors associated with it and the role of skin testing in its diagnosis. METHODS:A structured questionnaire was administered to patients who underwent computed tomography during ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000320760
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:Secretory phospholipases A(2) (sPLA(2)) are an emerging class of mediators of inflammation. These enzymes are released in vivo in patients with systemic inflammatory diseases and allergic disorders. sPLA(2)s may activate inflammatory cells by both enzymatic and nonenzymatic mechanisms. The aim of this study ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000218117
更新日期:2009-01-01 00:00:00
abstract::Human mononuclear leukocytes (MNL), probably OKT4-positive T cells, produced an eosinophil chemotactic factor (ECF) when they were cocultured with irradiated BALL-1, a B cell lymphoma line. Treatment of MNL, with anti-IL-2 antibody failed to suppress BALL-1-induced ECF production. Periodate-lysine-paraformaldehyde-fix...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236736
更新日期:1994-01-01 00:00:00
abstract:BACKGROUND:Clustered severe adverse reactions to immunotherapy with olive pollen extracts have been occasionally reported in areas where olive trees are extensively grown. Allergic patients from these areas, in addition to the major olive pollen allergen Ole e 1, frequently recognize a recently described allergen, Ole ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000092532
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND:Little information is available on eosinophil activation and the cytokine profile in virus-induced acute exacerbation of bronchial asthma; therefore, we examined the effects of treatments that included systemic corticosteroids on serum eosinophil cationic protein (ECP) and 17 cytokines/chemokines in rhinovir...
journal_title:International archives of allergy and immunology
pub_type: 临床试验,杂志文章
doi:10.1159/000327434
更新日期:2011-01-01 00:00:00
abstract::Cross-linking of FcepsilonRI on mast cells elicits positive signal transduction cascades that lead to the release of a variety of proinflammatory mediators. Mouse mast cells also express gp49B1 on their surface, an immunoglobulin superfamily member that bears two immunoreceptor tyrosine-based inhibitory motifs in its ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000024059
更新日期:1999-02-01 00:00:00
abstract:BACKGROUND:Anti-inflammatory therapy is a logical approach to slowing the inevitable lung function deterioration in cystic fibrosis (CF) patients. This study's aim was to evaluate inflammatory markers and disease progression in paediatric CF patients chronically treated with azithromycin or low-dose prednisolone. METH...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000453451
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:Numerous strategies have been proposed for the treatment of peanut allergies, but despite the steady advancement in our understanding of atopic immune responses and the increasing number of deaths each year from peanut anaphylaxis, there is still no safe, effective, specific therapy for the peanut-sensitive ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000053672
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:Diagnosis and immunotherapy of house-dust mite (HDM) allergy is still based on natural allergen extracts. The aim of this study was to analyze commercially available Dermatophagoides pteronyssinus extracts from different manufacturers regarding allergen composition and content and whether variations may affe...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000337654
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:Atopic dermatitis (AD) is increasing among the elderly. Staphylococcus aureus colonization is one of the most important aggravating factors in AD. OBJECTIVE:This study analyses the clinical features of AD in elderly patients and determines the pathogenic roles of staphylococcal superantigens in AD with low ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000329150
更新日期:2012-01-01 00:00:00
abstract::Transforming growth factor-beta 1 (TGF-beta(1)) is a key regulator of immune tolerance. TGF-beta(1) controls T lymphocyte activation and is involved in the immunosuppressive function and generation of regulatory T lymphocytes. Connective tissue growth factor (CTGF) has an essential role in the formation of connective ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000137284
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND:Exposure to Aspergillus fumigatus allergens results in the sensitization and the development of allergic bronchopulmonary aspergillosis in susceptible individuals. Aspergillus antigen consists of a number of chemically diverse components and their cumulative or synergistic effect may result in disease. When ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000065882
更新日期:2002-10-01 00:00:00
abstract::We describe an HLA-B8, DR3-positive patient with localized morphea after silicone gel breast implantation for cosmetic reasons. We believe that this case suggests that a genetic background, i.e. HLA-B8, DR3 haplotype, is involved in the autoimmune response to silicone. ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237437
更新日期:1997-01-01 00:00:00
abstract::Two cDNA clones named P9* and P1* of 794 and 631 bp, respectively, were isolated from a lambda ZAP cDNA expression library using Parietaria judaica (Pj) pollen-specific IgE antibodies from a pool of sera (n = 23) of patients allergic to Pj. Sequence analysis showed open reading frames of 176 and 138 amino acids. Both ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237479
更新日期:1997-04-01 00:00:00
abstract::Eosinophils participate in the inflammatory response seen in allergy and helminthic infestation. Their release of granule-bound cationic proteins may play a role in these diseases. Therefore, we investigated mechanisms involved in the release of eosinophil cationic protein (ECP). Serum-opsonized zymosan was phagocytos...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000023891
更新日期:1998-02-01 00:00:00
abstract::We examined the capacity of interleukin-4 (IL) production from lymphocytes and basophils, isolated from the peripheral blood of allergic patients sensitive to house dust mite, after stimulation with mite extract. IL-4 production was measured by a sensitive bioassay based on coculture with CT.h4S (a human IL-4-responsi...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237375
更新日期:1996-11-01 00:00:00
abstract:BACKGROUND:The 2S albumins are a group of storage proteins that occur widely in seeds of dicotyledonous plants. The widespread distribution and stability to digestion of allergenic 2S albumins raise the question of why some members of this family present in important food sources, such as soybean, are not regarded as m...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000094535
更新日期:2006-01-01 00:00:00
abstract::The present study has extended histochemical and functional investigations into rat mast cell heterogeneity using isolated mast cells from four connective tissue locations; the peritoneum, mesentery, lung and skin. On histological examination, mast cells from these locations displayed a range of phenotypes following f...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236161
更新日期:1992-01-01 00:00:00
abstract::Drug hypersensitivity reactions (DHR) are clinically and functionally heterogeneous. Different subclassifications based on timing of symptom appearance or type of immune mechanism have been proposed. Here, we show that the mode of action of drugs leading to immune/inflammatory cell stimulation is a further decisive fa...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
doi:10.1159/000453265
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:We reported on the constitutive interferon-gamma receptor (IFN-gammaR) expression on eosinophils. But signal transduction through IFN-gammaR on eosinophils remains to be elucidated. In this study, we examined the involvement of the Jak/Stat pathway in the signaling of eosinophils after IFN-gammaR conjugation...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000024159
更新日期:1999-02-01 00:00:00
abstract::Elevated levels of ovine reaginic antibody were induced by immunization with Ascaris suum antigens. The PCA activity of this antibody persisted in the skin of sheep for 20 days and was abolished by heating to 56 degrees C, suggesting that it was immunoglobulin E. Attempts to isolate IgE from this hyperimmune serum by ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236479
更新日期:1993-01-01 00:00:00