Probing the structural and functional domains of the CFTR chloride channel.

abstract：:We have studied a mutant strain of Drosophila in which 80% of the mitochondrial DNA molecules have lost over 30% of their coding region through deletion. This deletion affects genes encoding five subunits of complex I of the respiratory chain (NADH:ubiquinone oxidoreductase). The enzymatic activity of complex I in the...

journal_title：Journal of bioenergetics and biomembranes

authors： Farge G,Touraille S,Lachaume P,Debise R,Procaccio V,Alziari S

更新日期：2004-04-01 00:00:00

abstract：:Ferrochelatase [heme synthase, protoheme ferrolyase (EC 4.99.1.1)], the terminal enzyme of the heme biosynthetic pathway, catalyzes the incorporation of ferrous ion into protoporphyrin IX to form protoheme IX. The genes and cDNAs for ferrochelatase from mammals and micro-organisms have been isolated. The gene for huma...

journal_title：Journal of bioenergetics and biomembranes

authors： Taketani S,Fujita H

更新日期：1995-04-01 00:00:00

abstract：:We have previously shown that vacuolar H+-ATPase subcomplex V(o) from mung bean contains subunit d, however, its sequence and function were unknown. In the present study, we report the cloning and recombinant over expression of subunit d from mung bean in E. coli. To study the function of subunit d, two vacuolar H+-AT...

journal_title：Journal of bioenergetics and biomembranes

authors： Ouyang Z,Li Z,Zhang X

更新日期：2008-12-01 00:00:00

abstract：:The Mg2+ dependent asymmetry of the F(1)-ATPase catalytic sites leads to the differences in affinity for nucleotides and is an essential component of the binding-change mechanism. Changes in metal ligands during the catalytic cycle responsible for this asymmetry were characterized by vanadyl (V(IV) + O)2+, a functiona...

journal_title：Journal of bioenergetics and biomembranes

authors： Frasch WD

更新日期：2000-10-01 00:00:00

abstract：:A series of amphiphilic polymethlyenecarboxymaleimides has been synthesized for use as sulfhydryl reagents applicable to membrane proteins. Physical properties of the compounds which are relevant to their proposed mode of action have been determined. By comparing rates of reaction in aqueous and aprotic solvents, the ...

journal_title：Journal of bioenergetics and biomembranes

authors： Griffiths DG,Partis MD,Churchill P,Brenner SC,Fleischer S,Moore RJ,Beechey RB

更新日期：1990-10-01 00:00:00

abstract：:Traditionally, mitochondria have been viewed as the "powerhouse" of the cell, i.e., the site of the oxidative phosphorylation machinery involved in ATP production. Consequently, much of the research conducted on mitochondria over the past 4 decades has focused on elucidating both those molecular events involved in ATP...

journal_title：Journal of bioenergetics and biomembranes

authors： Pedersen PL

更新日期：1999-08-01 00:00:00

abstract：:Specific molecular interactions that determine many of the functions of a biomembrane have a high probability of occurring at the surface of that membrane. However, unlike their hydrophobic core, the polar-apolar interface of biomembranes has been somewhat neglected experimentally. Reasons for this are that the chemic...

journal_title：Journal of bioenergetics and biomembranes

authors： Watts A

更新日期：1987-12-01 00:00:00

abstract：:Two kinds of ATP binding sites were found on the ATPase molecule in deoxycholic acid-treated sarcoplasmic reticulum. One was the catalytic site (1 mol/mol active site) and its affinity was high. Upon addition of Ca2+, all the ATP bound to the catalytic site disappeared at 75 mM KCl, while a significant amount of ATP r...

journal_title：Journal of bioenergetics and biomembranes

authors： Nakamura Y,Tonomura Y

更新日期：1982-12-01 00:00:00

abstract：:Recent studies of isolated muscle membrane have enabled induction and monitoring of rapid Ca2+ release from sarcoplasmic reticulum (SR)5 in vitro by a variety of methods. On the other hand, various proteins that may be directly or indirectly involved in the Ca2+ release mechanism have begun to be unveiled. In this min...

journal_title：Journal of bioenergetics and biomembranes

authors： Ikemoto N,Ronjat M,Mészáros LG

更新日期：1989-04-01 00:00:00

abstract：:ATP binding cassette (ABC) transporters comprise an extended protein family involved in the transport of a broad spectrum of solutes across membranes. They consist of a common architecture including two ATP-binding domains converting chemical energy into conformational changes and two transmembrane domains facilitatin...

journal_title：Journal of bioenergetics and biomembranes

authors： Nikles D,Tampé R

更新日期：2007-12-01 00:00:00

abstract：:The chronology of the major discoveries important in elucidating certain aspects of the molecular basis of transmembrane signaling is briefly reviewed. Recent developments linking cell stimulation and transformation are intimated. ...

journal_title：Journal of bioenergetics and biomembranes

authors： Badwey JA

更新日期：1991-02-01 00:00:00

abstract：:Since the nineteenth century, a great amount of different biological structures and processes have been assessed by fluorescent dyes. Along with the uses of these compounds as vital and histological dyes, some fluorescent dyes have become valuable tools for the study of the pore phenomenon in plasma membranes. Some io...

journal_title：Journal of bioenergetics and biomembranes

authors： Ferreira L,Pereira L,Faria R

更新日期：2015-08-01 00:00:00

abstract：:The state-3 rate of respiration of potato tuber mitochondria is inhibited by concentrations of KCl or NaCl above 125 mM, and by concentrations of sucrose, lactose, or maltose above 500 mM, but not at all by mannitol, glucose, glycine, or proline up to a concentration of 1500 mM in the medium. Mitochondria from caulifl...

journal_title：Journal of bioenergetics and biomembranes

authors： Campbell LC,Raison JK,Brady CJ

更新日期：1976-06-01 00:00:00

abstract：:The formation of paracrystalline inclusions in mitochondria following postmortem aging in situ is further investigated. Inclusions are found to localize either in the mitochondrial intracristal space or between the inner and outer membrane. The greater majority of the inclusions are of the intracristal type. pH is not...

journal_title：Journal of bioenergetics and biomembranes

authors： Cheah KS,Cheah AM

更新日期：1977-04-01 00:00:00

abstract：:The iron-sulfur protein of the cytochrome bc1 complex oxidizes ubiquinol at center P in the protonmotive Q cycle mechanism, transferring one electron to cytochrome c1 and generating a low-potential ubisemiquinone anion which reduces the low-potential cytochrome b-566 heme group. In order to catalyze this divergent tra...

journal_title：Journal of bioenergetics and biomembranes

authors： Graham LA,Brandt U,Sargent JS,Trumpower BL

更新日期：1993-06-01 00:00:00

abstract：:The mitochondrial UPR (UPRmt) is rapidly gaining attention. While most studies on the UPRmt have focused on its role in aging, emerging studies suggest an important role of the UPRmt in cancer. Further, several of the players of the UPRmt in mammalian cells have well reported roles in the maintenance of the organelle....

journal_title：Journal of bioenergetics and biomembranes

authors： Kenny TC,Germain D

更新日期：2017-08-01 00:00:00

abstract：:The sarcoplasmic reticulum (SR) of skeletal muscle controls the contraction-relaxation cycle by raising and lowering the myoplasmic free-Ca2+ concentration. The coupling between excitation, i.e., depolarization of sarcolemma and transverse tubule (TT) and Ca2+ release from the terminal cisternae (TC) of SR takes place...

journal_title：Journal of bioenergetics and biomembranes

authors： Volpe P

更新日期：1989-04-01 00:00:00

abstract：:For the past seven years or so, much discussion and controversy in the field of human evolution has revolved around the application and interpretation of studies of human mitochondrial DNA variation, particularly the hypothesis that all mtDNA types in contemporary populations can be traced back to a single African anc...

journal_title：Journal of bioenergetics and biomembranes

authors： Stoneking M

更新日期：1994-06-01 00:00:00

abstract：:This study was designed to investigate the energetics of isolated rat hepatocyte swelling due to sodium-cotransported amino acid accumulation in a medium containing either glucose or octanoate as basal substrate. We show that the size of the increase in cytosolic volume is directly correlated with the total amino acid...

journal_title：Journal of bioenergetics and biomembranes

authors： Espié P,Devin A,Guérin B,Rigoulet M

更新日期：1997-12-01 00:00:00

abstract：:The mitochondrial ATP synthase is a membrane protein complex which couples the proton gradient across the mitochondrial inner membrane to the synthesis of ATP from ADP+Pi. The complex is composed of essential subunits for its motor functions and supernumerary subunits, the roles of which remain to be elucidated. Subun...

journal_title：Journal of bioenergetics and biomembranes

authors： Hong S,Pedersen PL

更新日期：2004-12-01 00:00:00

abstract：:Increased membrane unsaturation has been associated with shorter longevity due to higher sensitivity to lipid peroxidation (LP) leading to enhanced mitochondrial dysfunction and ROS overproduction. However, the role of LP during aging has been put in doubt along with the participation of electron leak at the electron ...

journal_title：Journal of bioenergetics and biomembranes

authors： Aguilar-Toral R,Fernández-Quintero M,Ortiz-Avila O,de la Paz LH,Calderón-Cortés E,Rodríguez-Orozco AR,Saavedra-Molina A,Calderón-Torres M,Cortés-Rojo C

更新日期：2014-06-01 00:00:00

abstract：:In Propionigenium modestum, ATP is manufactured from ADP and phosphate by the enzyme ATP synthase using the free energy of an electrochemical gradient of Na+ ions. The P. modestum ATP synthase is a clear member of the family of F-type ATP synthases and the only major distinction is an extension of the coupling ion spe...

journal_title：Journal of bioenergetics and biomembranes

authors： Dimroth P,Matthey U,Kaim G

更新日期：2000-10-01 00:00:00

abstract：:Reconstitution experiments were performed on lipid bilayer membranes in the presence of purified mitochondrial porin from yeast and of detergent-solubilized mitochondrial outer membranes of a porin-free yeast mutant. The addition of the porin resulted in a strong increase of the membrane conductance, which was caused ...

journal_title：Journal of bioenergetics and biomembranes

authors： Benz R,Schmid A,Dihanich M

更新日期：1989-08-01 00:00:00

abstract：:N- and P/Q-type calcium channels are localized in high density in presynaptic nerve terminals and are crucial elements in neuronal excitation-secretion coupling. In addition to mediating Ca2+ entry to initiate transmitter release, they are thought to interact directly with proteins of the synaptic vesicle docking/fusi...

journal_title：Journal of bioenergetics and biomembranes

authors： Sheng ZH,Westenbroek RE,Catterall WA

更新日期：1998-08-01 00:00:00

abstract：:Porin or voltage-dependent anion-selective channel (VDAC) is the main protein responsible for the high permeability of the outer mitochondrial membrane. The mitochondrial porin is mainly composed of sided beta-strands, in analogy with bacterial porin, whose structure has been resolved at 1.8 A resolution. In mitochond...

journal_title：Journal of bioenergetics and biomembranes

authors： De Pinto VD,Palmieri F

更新日期：1992-02-01 00:00:00

abstract：:Hydroxyl radical (HO•) production in photosystem II (PSII) was studied by electron paramagnetic resonance (EPR) spin-trapping technique. It is demonstrated here that the exposure of PSII membranes to heat stress (40 °C) results in HO• formation, as monitored by the formation of EMPO-OH adduct EPR signal. The presence ...

journal_title：Journal of bioenergetics and biomembranes

authors： Yadav DK,Pospíšil P

更新日期：2012-06-01 00:00:00

abstract：:The advantages of using bacterial systems to study the mechanism and function of cytochrome bc (1) complexes do not extend readily to their structural investigations. High quality crystals of bacterial complexes have been difficult to obtain despite the enzymes' smaller sizes and simpler subunit compositions compared ...

journal_title：Journal of bioenergetics and biomembranes

authors： Xia D,Esser L,Elberry M,Zhou F,Yu L,Yu CA

更新日期：2008-10-01 00:00:00

abstract：:Affinity labeling and genetic studies on the glycine-rich sequence of the beta subunit of E. coli F-type ATPase are discussed. A model of the structure of the enzyme near the gamma phosphate moiety is proposed. ...

journal_title：Journal of bioenergetics and biomembranes

authors： Futai M,Iwamoto A,Omote H,Maeda M

更新日期：1992-10-01 00:00:00

abstract：:The oxidation of NADH by mouse liver plasma membranes was shown to be accompanied by the formation of H2O2. The rate of H2O2 formation was less than one-tenth the rate of oxygen uptake and much slower than the rate of reduction of artificial electron acceptors. The optimum pH for this reaction was 7.0 and the Km value...

journal_title：Journal of bioenergetics and biomembranes

authors： Ramasarma T,Swaroop A,MacKellar W,Crane FL

更新日期：1981-12-01 00:00:00

abstract：:Reaction of a dog kidney (Na + K)-ATPase with pyridoxal phosphate, followed by borohydride reduction, reduced the catalytic activity when measured subsequently. The time course of inactivation did not follow a first-order process, and certain characteristics of the residual enzymatic activity were modified. Moreover, ...

journal_title：Journal of bioenergetics and biomembranes

authors： Robinson JD

更新日期：1984-06-01 00:00:00