Abstract：
:The advantages of using bacterial systems to study the mechanism and function of cytochrome bc (1) complexes do not extend readily to their structural investigations. High quality crystals of bacterial complexes have been difficult to obtain despite the enzymes' smaller sizes and simpler subunit compositions compared to their mitochondrial counterparts. In the course of the structure determination of the bc (1) complex from R. sphaeroides, we observed that the growth of only low quality crystals correlated with low activity and stability of the purified complex, which was mitigated in part by introducing a double mutations to the enzyme. The S287R(cyt b)/V135S(ISP) mutant shows 40% increase in electron transfer activity and displays a 4.3 degrees C increase in thermal stability over wild-type enzyme. The amino acid histidine was found important in maintaining structural integrity of the bacterial complex, while the respiratory inhibitors such as stigmatellin are required for immobilization of the iron-sulfur protein extrinsic domain. Crystal quality of the R. sphaeroides bc (1) complex can be improved further by the presence of strontium ions yielding crystals that diffracted X-rays to better than 2.3 A resolution. The improved crystal quality can be understood in terms of participation of strontium ions in molecular packing arrangement in crystal.
journal_name
J Bioenerg Biomembrjournal_title
Journal of bioenergetics and biomembranesauthors
Xia D,Esser L,Elberry M,Zhou F,Yu L,Yu CAdoi
10.1007/s10863-008-9180-8subject
Has Abstractpub_date
2008-10-01 00:00:00pages
485-92issue
5eissn
0145-479Xissn
1573-6881journal_volume
40pub_type
杂志文章abstract：:A sodium-dependent phosphate transporter (type II Na/Pi-cotransporter) was isolated which is expressed in apical membranes of proximal tubules and exhibits transport characteristics similar as described for renal reabsorption of phosphate. Type II associated Na/Pi-cotransport is electrogenic and results obtained by el...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1023/a:1020525409894
更新日期：1998-04-01 00:00:00
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journal_title：Journal of bioenergetics and biomembranes
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doi：10.1007/BF00744683
更新日期：1980-08-01 00:00:00
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journal_title：Journal of bioenergetics and biomembranes
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doi：10.1007/s10863-012-9454-z
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journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1023/a:1024641413205
更新日期：2003-06-01 00:00:00
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journal_title：Journal of bioenergetics and biomembranes
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doi：10.1007/BF00768540
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journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/s10863-016-9693-5
更新日期：2017-04-01 00:00:00
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journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
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更新日期：2017-08-01 00:00:00
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journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/BF00744687
更新日期：1980-08-01 00:00:00
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doi：10.1007/BF00762786
更新日期：1994-08-01 00:00:00
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journal_title：Journal of bioenergetics and biomembranes
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doi：10.1007/s10863-006-9042-1
更新日期：2006-08-01 00:00:00
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journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1023/a:1022482923122
更新日期：1997-10-01 00:00:00
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journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1023/a:1005448614450
更新日期：1999-02-01 00:00:00
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journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/BF00748958
更新日期：1976-06-01 00:00:00
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journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
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更新日期：2008-04-01 00:00:00
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journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1023/a:1021985521748
更新日期：1998-08-01 00:00:00
abstract：:The cytochrome b6/f complex of higher plant chloroplasts is uniformly distributed throughout both appressed and nonappressed thylakoids, in contrast to photosystem II and photosystem I, the other major membrane protein complexes involved in electron transport. We discuss how this distribution is likely to affect inter...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1007/BF00743013
更新日期：1986-10-01 00:00:00
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journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1023/a:1010615508916
更新日期：2001-08-01 00:00:00
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journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1023/a:1005488105076
更新日期：1999-10-01 00:00:00
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journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
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更新日期：1999-10-01 00:00:00
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journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/s10863-018-9766-8
更新日期：2018-10-01 00:00:00
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journal_title：Journal of bioenergetics and biomembranes
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更新日期：2007-06-01 00:00:00
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journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
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更新日期：2019-06-01 00:00:00
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journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1023/a:1005471829569
更新日期：1999-06-01 00:00:00
abstract：:Calcium may function directly in several aspects of photosynthesis. It appears to modulate activity of the phosphatase enzymes in the carbon reduction cycle and also to regulate chloroplast NAD+ kinase activity through a calmodulin-like protein. Some evidence supports a calcium function in the water-splitting complex,...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1007/BF00744278
更新日期：1984-08-01 00:00:00
abstract：:Hepatic iron toxicity because of iron overload seems to be mediated by lipid peroxidation of biological membranes and the associated organelle dysfunctions. However, the basic mechanisms underlying this process in vivo are still little understood. Gerbils were dosed with weekly injections of iron-dextran alone or in c...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1023/a:1005512014280
更新日期：2000-04-01 00:00:00
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journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/BF00744524
更新日期：1983-10-01 00:00:00
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journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/s10863-010-9276-9
更新日期：2010-04-01 00:00:00
abstract：:Recent studies of isolated muscle membrane have enabled induction and monitoring of rapid Ca2+ release from sarcoplasmic reticulum (SR)5 in vitro by a variety of methods. On the other hand, various proteins that may be directly or indirectly involved in the Ca2+ release mechanism have begun to be unveiled. In this min...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1007/BF00812071
更新日期：1989-04-01 00:00:00
abstract：:Hundreds of proteins in the nervous system are modified by the monosaccharide O-GlcNAc. A single protein is often O-GlcNAcylated on several amino acids and the modification of a single site can play a crucial role for the function of the protein. Despite its complexity, only two enzymes add and remove O-GlcNAc from pr...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1007/s10863-018-9760-1
更新日期：2018-06-01 00:00:00
abstract：:Cytoplasmic male sterility arises when mitochondrial activities are disrupted that are essential for pollen development. Rearrangements in the mitochondrial genome that create novel open reading frames are strongly correlated with CMS phenotypes in a number of systems. The morphological aberrations which indicate CMS-...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1007/BF02110007
更新日期：1995-08-01 00:00:00
