Abstract：
:Direct and indirect evidences show that elevated glucose concentrations in airway surface liquid (ASL) promote lung infection by pathogens, playing a role in the progression of the Cystic Fibrosis (CF) disease. The joint action of transporter/s for glucose and of the cellular enzymes is essential in order to try to lower ASL glucose level. Inside the cell, the glycolysis and the pentose phosphate pathway (PPP) compete for the utilization of glucose-6-phosphate (G6P), the product in which glucose, after entry within the cell and phosphorylation, is trapped. The study aims to clarify whether, modulating the activity of enzymatic proteins and/or the level of metabolites/cofactors, involved in intracellular glucose utilization, a lowering of the extracellular glucose level in CF occurs. Biochemical approaches have enabled us to understand i) how G6P is shunted between glycolysis and PPP and ii) that mitochondria, more than enzymes/cofactors participating to the two cell glucose utilization pathways, are protagonists of the scene in counteracting the high ASL glucose level as well as oxidative stress in CF.
journal_name
J Bioenerg Biomembrjournal_title
Journal of bioenergetics and biomembranesauthors
Favia M,de Bari L,Lassandro R,Atlante Adoi
10.1007/s10863-019-09797-5subject
Has Abstractpub_date
2019-06-01 00:00:00pages
203-218issue
3eissn
0145-479Xissn
1573-6881pii
10.1007/s10863-019-09797-5journal_volume
51pub_type
杂志文章abstract：:A sodium-dependent phosphate transporter (type II Na/Pi-cotransporter) was isolated which is expressed in apical membranes of proximal tubules and exhibits transport characteristics similar as described for renal reabsorption of phosphate. Type II associated Na/Pi-cotransport is electrogenic and results obtained by el...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1023/a:1020525409894
更新日期：1998-04-01 00:00:00
abstract：:As the terminal component of the mitochondrial respiratory chain, cytochrome c oxidase plays a vital role in cellular energy transformation. Human cytochrome c oxidase is composed of 13 subunits. The three major subunits form the catalytic core and are encoded by mitochondrial DNA (mtDNA). The remaining subunits are n...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1023/a:1022638013825
更新日期：1997-04-01 00:00:00
abstract：:The multi-subunit vacuolar-type H(+)-ATPase consists of a V(1) domain (A-H subunits) catalyzing ATP hydrolysis and a V(0) domain (a, c, c', c", d, e) responsible for H(+) translocation. The mammalian V(0) d subunit is one of the least-well characterized, and its function and position within the pump are still unclear....
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/s10863-008-9161-y
更新日期：2008-08-01 00:00:00
abstract：:Dysfunction of neuronal metabolism is central to all forms of seizure activity. Clinical, genetic and metabolic studies have implicated mitochondria in the process. Alterations in electron transport, generation of reactive oxygen species, calcium metabolism and apoptosis all have been described in human and animal ner...
journal_title：Journal of bioenergetics and biomembranes
pub_type：
doi：10.1007/s10863-010-9322-7
更新日期：2010-12-01 00:00:00
abstract：:Although the "Warburg effect", i.e., elevated glucose metabolism to lactic acid (glycolysis) even in the presence of oxygen, has been recognized as the most common biochemical phenotype of cancer for over 80 years, its biochemical and genetic basis remained unknown for over 50 years. Work focused on elucidating the un...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 社论
doi：10.1007/s10863-012-9425-4
更新日期：2012-02-01 00:00:00
abstract：:The P515 absorbance change upon single-turnover light flashes has been studied in intact leaves and isolated chloroplasts from spinach. A comparative study of the effects of preillumination on the kinetics of the P515 response and on the activity of the chloroplast ATPase has been made. The slow component (reaction 2)...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/BF00751054
更新日期：1983-12-01 00:00:00
abstract：:Changes in protein and fatty acid compositions of flounder sarcoplasmic reticulum during NADH plus ascorbate-dependent lipid peroxidation in vitro were related to the ability of the sarcoplasmic reticulum to sequester Ca+2. Progressive accumulation of high-molecular-weight protein components occurred concomitantly wit...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/BF00743052
更新日期：1986-08-01 00:00:00
abstract：:Many previous investigations have consistently reported that caloric restriction (40%), which increases maximum longevity, decreases mitochondrial reactive species (ROS) generation and oxidative damage to mitochondrial DNA (mtDNA) in laboratory rodents. These decreases take place in rat liver after only seven weeks of...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/s10863-006-9051-0
更新日期：2006-12-01 00:00:00
abstract：:We have studied changes in plasma membrane NAD(P)H:quinone oxidoreductases of HL-60 cells under serum withdrawal conditions, as a model to analyze cell responses to oxidative stress. Highly enriched plasma membrane fractions were obtained from cell homogenates. A major part of NADH-quinone oxidoreductase in the plasma...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1023/a:1016035504049
更新日期：2002-06-01 00:00:00
abstract：:The adenylate kinase (AK) isoforms network plays an important role in the intracellular energy transfer processes, the maintenance of energy homeostasis, and it is a major player in AMP metabolic signaling circuits in some highly-differentiated cells. For this purpose, a rapid and sensitive method was developed that e...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/s10863-016-9687-3
更新日期：2016-10-01 00:00:00
abstract：:Yeasts capable of growing and surviving at high temperatures are regarded as thermotolerant. For appropriate functioning of cellular processes and cell survival, the maintenance of an optimal redox state is critical of reducing and oxidizing species. We studied mitochondrial functions of the thermotolerant Kluyveromyc...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/s10863-017-9696-x
更新日期：2017-04-01 00:00:00
abstract：:Recent advances in structural biology underlying mechanisms of channel gating have strengthened our knowledge about how K(+) channels can be inter-convertible between conductive and non-conductive states. We have reviewed and combined mutagenesis with biochemical, biophysical and structural information in order to und...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1007/s10863-012-9407-6
更新日期：2012-02-01 00:00:00
abstract：:Evidence suggests membrane bound F(1)F(0)-ATPase complexes form stable associations such that dimers can be retrieved from detergent lysates of mitochondria isolated from a range of sources including algae, higher plants, yeast and bovine heart, and plant chloroplasts. The physiological relevance of these interactions...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/s10863-005-4128-8
更新日期：2005-04-01 00:00:00
abstract：:Cyclosporin A (CsA) is highly neuroprotective in several animal models of acute neurological damage and neurodegenerative disease with inhibition of the mitochondrial permeability transition (mPT) having emerged as a possible mechanism for the observed neuroprotection. In the present study, we have evaluated two new n...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1023/B:JOBB.0000041776.31885.45
更新日期：2004-08-01 00:00:00
abstract：:Mitochondria are dynamic structures for which fusion and fission are well characterized for rapidly dividing cells in culture. Based on these data, it has recently been proposed that high respiratory activity is the result of fusion and formation of mitochondrial reticulum, while fission results in fragmented mitochon...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1007/s10863-012-9494-4
更新日期：2013-08-01 00:00:00
abstract：:In order to determine whether polymorphic forms of the Ca2+ + Mg2+-dependent ATPase exist, we have examined the cross-reactivity of five monoclonal antibodies prepared against the rabbit skeletal muscle sarcoplasmic reticulum enzyme with proteins from microsomal fractions isolated from a variety of muscle and nonmuscl...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/BF00743238
更新日期：1984-12-01 00:00:00
abstract：:Little is known on antimicrobial peptide permeation through outer membrane channels in gram-negative bacteria. Herein, we probed at a single-molecule level the interaction of two different peptides, magainin 2 and HPA3P with OmpF from E. coli. HPA3P is an analogue of the antimicrobial peptide HP(2-20) isolated from th...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/s10863-010-9273-z
更新日期：2010-04-01 00:00:00
abstract：:Recent progress is summarized on the structure, function, and regulation of the tricarboxylate (i.e., citrate) transport protein (CTP) from the rat liver mitochondrial inner membrane. The transporter has been purified and its reconstituted function characterized. A cDNA clone encoding the CTP has been isolated and seq...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/BF01108407
更新日期：1993-10-01 00:00:00
abstract：:The state-3 rate of respiration of potato tuber mitochondria is inhibited by concentrations of KCl or NaCl above 125 mM, and by concentrations of sucrose, lactose, or maltose above 500 mM, but not at all by mannitol, glucose, glycine, or proline up to a concentration of 1500 mM in the medium. Mitochondria from caulifl...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/BF00748958
更新日期：1976-06-01 00:00:00
abstract：:Bioenergetics of tomato (Lycopersicon esculentum) development on the plant was followed from the early growing stage to senescence in wild type (climacteric) and nonripening mutant (nor, non-climacteric) fruits. Fruit development was expressed in terms of evolution of chlorophyll a content allowing the assessment of a...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1023/a:1022574327117
更新日期：2002-12-01 00:00:00
abstract：:It is proven that any model of localized protonmotive energy coupling that relies upon properties of a homogeneous surface phase must, when operated in the steady state, lead to bulk phase electrochemical potentials for protons that are as large as those required by the delocalized chemiosmotic theory. To obtain model...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1007/BF00743612
更新日期：1986-02-01 00:00:00
abstract：:Cytochrome c oxidase was purified from mitochondria of Euglena gracilis and separated into 15 different polypeptide subunits by polyacrylamide gel electrophoresis. All 15 subunits copurify through various purification procedures, and the subunit composition of the isolated enzyme is identical to that of the immunoprec...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/BF00762727
更新日期：1989-06-01 00:00:00
abstract：:Available data indicate that minocycline, an antibiotic of the tetracycline family, has cytoprotective properties due to a direct interaction with mitochondria. Yet, the data in the case of isolated mitochondria suggest discrepant or even detrimental effect(s) of the interaction. We have studied the cytoprotective act...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/s10863-012-9441-4
更新日期：2012-06-01 00:00:00
abstract：:N- and P/Q-type calcium channels are localized in high density in presynaptic nerve terminals and are crucial elements in neuronal excitation-secretion coupling. In addition to mediating Ca2+ entry to initiate transmitter release, they are thought to interact directly with proteins of the synaptic vesicle docking/fusi...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1023/a:1021985521748
更新日期：1998-08-01 00:00:00
abstract：:Escherichia coli has four [NiFe]-hydrogenases (Hyd); three of these, Hyd-1, Hyd-2 and Hyd-3 have been characterized well. In this study the requirement for the F(0)F(1)-ATP synthase for the activities of the hydrogen-oxidizing hydrogenases Hyd-1 and Hyd-2 was examined. During fermentative growth on glucose at pH 7.5 a...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/s10863-011-9397-9
更新日期：2011-12-01 00:00:00
abstract：:Specific molecular interactions that determine many of the functions of a biomembrane have a high probability of occurring at the surface of that membrane. However, unlike their hydrophobic core, the polar-apolar interface of biomembranes has been somewhat neglected experimentally. Reasons for this are that the chemic...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1007/BF00762300
更新日期：1987-12-01 00:00:00
abstract：:Plasma membranes isolated from K562 cells contain an NADH-ascorbate free radical reductase activity and intact cells show the capacity to reduce the rate of chemical oxidation of ascorbate leading to its stabilization at the extracellular space. Both activities are stimulated by CoQ10 and inhibited by capsaicin and di...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1023/a:1022410127104
更新日期：1997-06-01 00:00:00
abstract：:Eukaryotic porins or VDACs are a class of transmembrane proteins mainly localized in the outer mitochondrial membrane, whose function is to allow the diffusion of metabolites between the cytosol and the mitochondrion. In Drosophila melanogaster, as in other organisms, a small family of genes encoding porins has been d...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/s10863-008-9149-7
更新日期：2008-06-01 00:00:00
abstract：:A series of amphiphilic polymethlyenecarboxymaleimides has been synthesized for use as sulfhydryl reagents applicable to membrane proteins. Physical properties of the compounds which are relevant to their proposed mode of action have been determined. By comparing rates of reaction in aqueous and aprotic solvents, the ...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/BF00809072
更新日期：1990-10-01 00:00:00
abstract：:In the mitochondrial F(O)F(1) ATP-synthase/ATPase complex, subunits α and β are part of the extrinsic portion that catalyses ATP synthesis. Since there are no reports about genes and proteins from these subunits in crustaceans, we analyzed the cDNA sequences of both subunits in the whiteleg shrimp Litopenaeus vannamei...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/s10863-011-9340-0
更新日期：2011-04-01 00:00:00
