Abstract：
:The adenylate kinase (AK) isoforms network plays an important role in the intracellular energy transfer processes, the maintenance of energy homeostasis, and it is a major player in AMP metabolic signaling circuits in some highly-differentiated cells. For this purpose, a rapid and sensitive method was developed that enables to estimate directly and semi-quantitatively the distribution between cytosolic AK1 and mitochondrial AK2 localized in the intermembrane space, both in isolated cells and tissue samples (biopsy material). Experiments were performed on isolated rat mitochondria or permeabilized material, including undifferentiated and differentiated neuroblastoma Neuro-2a cells, HL-1 cells, isolated rat heart cardiomyocytes as well as on human breast cancer postoperative samples. In these samples, the presence of AK1 and AK2 could be detected by high-resolution respirometry due to the functional coupling of these enzymes with ATP synthesis. By eliminating extra-mitochondrial ADP with an excess of pyruvate kinase and its substrate phosphoenolpyruvate, the coupling of the AK reaction with mitochondrial ATP synthesis could be quantified for total AK and mitochondrial AK2 as a specific AK index. In contrast to the creatine kinase pathway, the AK phosphotransfer pathway is up-regulated in murine neuroblastoma and HL-1 sarcoma cells and in these malignant cells expression of AK2 is higher than AK1. Differentiated Neuro-2a neuroblastoma cells exhibited considerably higher OXPHOS capacity than undifferentiated cells, and this was associated with a remarkable decrease in their AK activity. The respirometric method also revealed a considerable difference in mitochondrial affinity for AMP between non-transformed cells and tumor cells.
journal_name
J Bioenerg Biomembrjournal_title
Journal of bioenergetics and biomembranesauthors
Klepinin A,Ounpuu L,Guzun R,Chekulayev V,Timohhina N,Tepp K,Shevchuk I,Schlattner U,Kaambre Tdoi
10.1007/s10863-016-9687-3subject
Has Abstractpub_date
2016-10-01 00:00:00pages
531-548issue
5eissn
0145-479Xissn
1573-6881pii
10.1007/s10863-016-9687-3journal_volume
48pub_type
杂志文章abstract：:The presence of transplasma membrane electron transport in a variety of plant cells and tissues is reported. It is now agreed that this property of eukaryotic cells is of ubiquitous nature. Studies with highly purified plasma membranes have established the presence of electron transport enzymes. Two types of activitie...
journal_title：Journal of bioenergetics and biomembranes
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journal_title：Journal of bioenergetics and biomembranes
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journal_title：Journal of bioenergetics and biomembranes
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journal_title：Journal of bioenergetics and biomembranes
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journal_title：Journal of bioenergetics and biomembranes
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journal_title：Journal of bioenergetics and biomembranes
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journal_title：Journal of bioenergetics and biomembranes
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journal_title：Journal of bioenergetics and biomembranes
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journal_title：Journal of bioenergetics and biomembranes
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journal_title：Journal of bioenergetics and biomembranes
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journal_title：Journal of bioenergetics and biomembranes
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journal_title：Journal of bioenergetics and biomembranes
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journal_title：Journal of bioenergetics and biomembranes
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journal_title：Journal of bioenergetics and biomembranes
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journal_title：Journal of bioenergetics and biomembranes
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journal_title：Journal of bioenergetics and biomembranes
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更新日期：2020-08-01 00:00:00
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journal_title：Journal of bioenergetics and biomembranes
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更新日期：1977-08-01 00:00:00
abstract：:Overexpression of the Homo sapiens LYR motif containing 1 (LYRM1) causes mitochondrial dysfunction and induces insulin resistance in 3T3-L1 adipocytes. α-Lipoic acid (α-LA), a dithiol compound with antioxidant properties, improves glucose transport and utilization in 3T3-L1 adipocytes. The aim of this study was to inv...
journal_title：Journal of bioenergetics and biomembranes
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更新日期：2012-10-01 00:00:00
