Electron microscopic structural analysis of Photosystem I, Photosystem II, and the cytochrome b6/f complex from green plants and cyanobacteria.

Abstract:

:Electron microscopy (EM) in combination with image analysis is a powerful technique to study protein structure at low- and high resolution. Since electron micrographs of biological objects are very noisy, substantial improvement of image quality can be obtained by averaging individual projections. Crystallographic and noncrystallographic averaging methods are available and have been applied to study projections of the large protein complexes embedded in photosynthetic membranes from cyanobacteria and higher plants. Results of EM on monomeric and trimeric Photosystem I complexes, on monomeric and dimeric Photosystem II complexes, and on the monomeric cytochrome b6/f complex are discussed.

journal_name

J Bioenerg Biomembr

authors

Boekema EJ,Boonstra AF,Dekker JP,Rögner M

doi

10.1007/BF00763217

subject

Has Abstract

pub_date

1994-02-01 00:00:00

pages

17-29

issue

1

eissn

0145-479X

issn

1573-6881

journal_volume

26

pub_type

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