Abstract:
:Patch-clamp studies of the yeast Schizosaccharomyces pombe reveal that the plasma membrane contains a voltage-gated channel mildly selective for potassium over sodium, lithium, and chloride. The channel exhibits several conductances with a maximum of 153 pS. The channel gates in the region of physiologically relevant voltages, being closed at hyperpolarizing and open at depolarizing voltages. It is not inhibited by tetraethylammonium, quinine, or quinidine applied from the cytoplasmic side of the membrane; similarly, ATP and stretch have no effect. The frequency of its occurrence in patches implies that about 35 channels of this kind are present in the plasma membrane of a single cell.
journal_name
J Bioenerg Biomembrjournal_title
Journal of bioenergetics and biomembranesauthors
Vacata V,Höfer M,Larsson HP,Lecar Hdoi
10.1007/BF00768067subject
Has Abstractpub_date
1993-02-01 00:00:00pages
43-53issue
1eissn
0145-479Xissn
1573-6881journal_volume
25pub_type
杂志文章abstract::Conformational changes within the subunit b-dimer of the E. coli ATP synthase occur upon binding to the F(1) sector. ESR spectra of spin-labeled b at room temperature indicated a pivotal point in the b-structure at residue 62. Spectra of frozen b +/- F(1) and calculated interspin distances suggested that where contact...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/s10863-008-9189-z
更新日期:2008-12-01 00:00:00
abstract::The organization of the oxidative phosphorylation (OXPHOS) system within the inner mitochondrial membrane appears to be far more complicated than previously thought. In particular, the individual protein complexes of the OXPHOS system (complexes I to V) were found to specifically interact forming defined supramolecula...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1007/s10863-008-9167-5
更新日期:2008-10-01 00:00:00
abstract::Cytoplasmic organelles with an acidic luminal pH include vacuoles, coated vesicles, lysosomes, the Golgi apparatus, and synaptic vesicles. Acidic compartments are also known outside specialized cells such as osteoclasts. The unique acidic pH is formed by V-ATPase (Vacuolar type ATPase), other ion transporters, and the...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1023/a:1025780932403
更新日期:2003-08-01 00:00:00
abstract::Retinoic acid inhibits the reduction of diferric transferrin through the transplasma membrane electron transport system on fetal rat liver cells infected with a temperature-sensitive SV40 virus when the cells are in the nontransformed state cultured at 40 degrees C. When the cells are in the transformed state (grown a...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/BF00769639
更新日期:1988-06-01 00:00:00
abstract::Thylakoids are highly protein-enriched membranes that harbor a number of multicomponent photosynthetic complexes. Similarly to other biological membranes the protein constituents are heterogeneously distributed laterally in the plane of the membrane, however the specific segregation into stacked (grana patches) and un...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/s10863-018-9783-7
更新日期:2018-12-01 00:00:00
abstract::Recent progress regarding the structure of the Ca(2+)-translocating ATPase of sarcoplasmic reticulum in several conformational states, and a substantial accumulation of biochemical information about this and other P-type ATPases, have put everything in place for the final convergence of biochemistry and structure that...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1023/a:1024641413205
更新日期:2003-06-01 00:00:00
abstract::The bacterial phosphoenolpyruvate-dependent carbohydrate phosphotransferase system (PTS) consists of several proteins whose primary functions are to transport and phosphorylate their substrates. The complexity of the PTS undoubtedly reflects its additional roles in chemotaxis to PTS substrates and in regulation of oth...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1007/BF00770249
更新日期:1993-12-01 00:00:00
abstract::ATP1-111, a suppressor of the slow-growth phenotype of yme1Delta lacking mitochondrial DNA is due to the substitution of phenylalanine for valine at position 111 of the alpha-subunit of mitochondrial ATP synthase (Atp1p in yeast). The suppressing activity of ATP1-111 requires intact beta (Atp2p) and gamma (Atp3p) subu...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/s10863-007-9071-4
更新日期:2007-04-01 00:00:00
abstract::Oxygen plays a key role in bacterial bioluminescence. The simultaneous and continuous kinetics of oxygen consumption and light emission during a complete exhaustion of the exogenous oxygen present in a closed system has been investigated. The kinetics are performed with Vibrio fischeri, V. harveyi, and Photobacterium ...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1023/a:1010615508916
更新日期:2001-08-01 00:00:00
abstract::Available data indicate that minocycline, an antibiotic of the tetracycline family, has cytoprotective properties due to a direct interaction with mitochondria. Yet, the data in the case of isolated mitochondria suggest discrepant or even detrimental effect(s) of the interaction. We have studied the cytoprotective act...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/s10863-012-9441-4
更新日期:2012-06-01 00:00:00
abstract::We investigated the effects of K(ATP) channel openers diazoxide and pinacidil on the respiration rate and membrane potential (deltapsi) of rat heart mitochondria, oxidizing pyruvate and malate. Diazoxide and pinacidil (58.8-1348.3 microM) increased the V2 (-ADP) respiration rate accordingly by 13-208% and 30-273% and ...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1023/a:1013870704002
更新日期:2002-02-01 00:00:00
abstract::The respiratory chain of marine and moderately halophilic bacteria requires Na+ for maximum activity, and the site of Na(+)-dependent activation is located in the NADH-quinone reductase segment. The Na(+)-dependent NADH-quinone reductase purified from marine bacterium Vibrio alginolyticus is composed of three subunits...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1007/BF00762464
更新日期:1993-08-01 00:00:00
abstract::Hundreds of proteins in the nervous system are modified by the monosaccharide O-GlcNAc. A single protein is often O-GlcNAcylated on several amino acids and the modification of a single site can play a crucial role for the function of the protein. Despite its complexity, only two enzymes add and remove O-GlcNAc from pr...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1007/s10863-018-9760-1
更新日期:2018-06-01 00:00:00
abstract::In order to determine whether polymorphic forms of the Ca2+ + Mg2+-dependent ATPase exist, we have examined the cross-reactivity of five monoclonal antibodies prepared against the rabbit skeletal muscle sarcoplasmic reticulum enzyme with proteins from microsomal fractions isolated from a variety of muscle and nonmuscl...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/BF00743238
更新日期:1984-12-01 00:00:00
abstract::The role of nucleoside diphosphate (NDP) kinase with special reference to mammalian signal transduction systems was described. The interaction between NDP kinases and G proteins was reevaluated in view of their protein structural information and its significance was extended further on the basis of recent findings obt...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1023/a:1023489722460
更新日期:2003-02-01 00:00:00
abstract::Little is known on antimicrobial peptide permeation through outer membrane channels in gram-negative bacteria. Herein, we probed at a single-molecule level the interaction of two different peptides, magainin 2 and HPA3P with OmpF from E. coli. HPA3P is an analogue of the antimicrobial peptide HP(2-20) isolated from th...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/s10863-010-9273-z
更新日期:2010-04-01 00:00:00
abstract::The topic of human adult neural plasticity and neurogenesis is of great interest for medical and scientific community, but it is also largely debated. In the last years, an increasing interest has been paid to the olfactory system, and particularly to the plasticity of the olfactory bulb (OB). While the molecular/cell...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1007/s10863-018-9780-x
更新日期:2019-02-01 00:00:00
abstract::Traumatic brain injury (TBI) is still the leading cause of disability in young adults worldwide. The major mechanisms - diffuse axonal injury, cerebral contusion, ischemic neurological damage, and intracranial hematomas have all been shown to be associated with mitochondrial dysfunction in some form. Mitochondrial dys...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1007/s10863-014-9589-1
更新日期:2015-04-01 00:00:00
abstract::The soxL gene from Sulfolobus acidocaldarius (DSM 639) encodes a Rieske iron-sulfur protein. In this study we report the identification of two open reading frames in its downstream region. The first one, named soxN, codes for a membrane protein bearing a resemblance to the b-type cytochromes of the cytochrome bc1 and ...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1023/a:1023742002493
更新日期:2003-04-01 00:00:00
abstract::Signal peptidases, the endoproteases that remove the amino-terminal signal sequence from many secretory proteins, have been isolated from various sources. Seven signal peptidases have been purified, two from E. coli, two from mammalian sources, and three from mitochondrial matrix. The mitochondrial enzymes are soluble...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1007/BF00763168
更新日期:1990-06-01 00:00:00
abstract::A sodium-dependent phosphate transporter (type II Na/Pi-cotransporter) was isolated which is expressed in apical membranes of proximal tubules and exhibits transport characteristics similar as described for renal reabsorption of phosphate. Type II associated Na/Pi-cotransport is electrogenic and results obtained by el...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1023/a:1020525409894
更新日期:1998-04-01 00:00:00
abstract::The Nm23-H1/nucleoside diphosphate (NDP) kinase A is a metastasis suppressor, besides its enzymatic activity. The mutant S120G has been found in high-grade neuroblastomas. The mutant protein, once denatured in urea, is unable to refold in vitro. A size-exclusion chromatography analysis of the folding/association pathw...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/s10863-006-9042-1
更新日期:2006-08-01 00:00:00
abstract::Cellular Ca2+ signals are crucial in the control of most physiological processes, cell injury and programmed cell death; mitochondria play a pivotal role in the regulation of such cytosolic Ca2+ ([Ca2+]c) signals. Mitochondria are endowed with multiple Ca2+ transport mechanisms by which they take up and release Ca2+ a...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1023/a:1005508311495
更新日期:2000-02-01 00:00:00
abstract::The present state of the chemiosmotic concept is reviewed. Special attention is paid to (i) further progress in studies on the Na(+)-coupled energetics and (ii) paradoxical bioenergetic effects when protonic or sodium potentials are utilized outside the coupling membrane (TonB-mediated uphill transports across the out...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1007/BF00831533
更新日期:1994-12-01 00:00:00
abstract::The multi-subunit vacuolar-type H(+)-ATPase consists of a V(1) domain (A-H subunits) catalyzing ATP hydrolysis and a V(0) domain (a, c, c', c", d, e) responsible for H(+) translocation. The mammalian V(0) d subunit is one of the least-well characterized, and its function and position within the pump are still unclear....
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/s10863-008-9161-y
更新日期:2008-08-01 00:00:00
abstract::Crude membrane preparations of a rho0 mutant of Saccharomyces cerevisiae exhibit Mg2+-dependent ATPase activity. Over the optimal pH range, 5.0-6.75, the apparent Vmax of the enzyme equals 590 nmoles of ATP hydrolyzed per minute per milligram protein, with an apparent Km for ATP of 1.3 mM. ATP hydrolysis is insensitiv...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/BF00744687
更新日期:1980-08-01 00:00:00
abstract::Phospholysine phosphohistidine inorganic pyrophosphate phosphatase (LHPP) has been recently identified as a novel inhibitor of multiple tumors; however, its role in glioblastoma (GBM) has not been investigated. This study aimed to evaluate whether LHPP exerts a potential tumor-inhibiting role in GBM. Compared with tha...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/s10863-020-09866-0
更新日期:2021-02-01 00:00:00
abstract::Beef heart cytochrome c oxidase was reconstituted in asolectin liposomes containing the pH indicator fluorescein-phosphatidylethanolamine (FPE) by the cholate-dialysis procedure. The influence of FPE on the asolectin liposome size and of the removal of subunit III from the complex on its incorporation into liposomes w...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/BF00743111
更新日期:1985-12-01 00:00:00
abstract::Hydroxyl radical (HO•) production in photosystem II (PSII) was studied by electron paramagnetic resonance (EPR) spin-trapping technique. It is demonstrated here that the exposure of PSII membranes to heat stress (40 °C) results in HO• formation, as monitored by the formation of EMPO-OH adduct EPR signal. The presence ...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/s10863-012-9433-4
更新日期:2012-06-01 00:00:00
abstract::The oxidation of NADH by mouse liver plasma membranes was shown to be accompanied by the formation of H2O2. The rate of H2O2 formation was less than one-tenth the rate of oxygen uptake and much slower than the rate of reduction of artificial electron acceptors. The optimum pH for this reaction was 7.0 and the Km value...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/BF00743203
更新日期:1981-12-01 00:00:00