Ionic channels in the plasma membrane of Schizosaccharomyces pombe: evidence from patch-clamp measurements.

Abstract:

:Patch-clamp studies of the yeast Schizosaccharomyces pombe reveal that the plasma membrane contains a voltage-gated channel mildly selective for potassium over sodium, lithium, and chloride. The channel exhibits several conductances with a maximum of 153 pS. The channel gates in the region of physiologically relevant voltages, being closed at hyperpolarizing and open at depolarizing voltages. It is not inhibited by tetraethylammonium, quinine, or quinidine applied from the cytoplasmic side of the membrane; similarly, ATP and stretch have no effect. The frequency of its occurrence in patches implies that about 35 channels of this kind are present in the plasma membrane of a single cell.

journal_name

J Bioenerg Biomembr

authors

Vacata V,Höfer M,Larsson HP,Lecar H

doi

10.1007/BF00768067

subject

Has Abstract

pub_date

1993-02-01 00:00:00

pages

43-53

issue

1

eissn

0145-479X

issn

1573-6881

journal_volume

25

pub_type

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