Conformational changes in the Escherichia coli ATP synthase b-dimer upon binding to F(1)-ATPase.


:Conformational changes within the subunit b-dimer of the E. coli ATP synthase occur upon binding to the F(1) sector. ESR spectra of spin-labeled b at room temperature indicated a pivotal point in the b-structure at residue 62. Spectra of frozen b +/- F(1) and calculated interspin distances suggested that where contact between b (2) and F(1) occurs (above about residue 80), the structure of the dimer changes minimally. Between b-residues 33 and 64 inter-subunit distances in the F(1)-bound b-dimer were found to be too large to accommodate tightly coiled coil packing and therefore suggest a dissociation and disengagement of the dimer upon F(1)-binding. Mechanistic implications of this "bubble" formation in the tether domain of ATP synthase b ( 2 ) are discussed.


J Bioenerg Biomembr


Zaida TM,Hornung T,Volkov OA,Hoffman AD,Pandey SJ,Wise JG,Vogel PD




Has Abstract


2008-12-01 00:00:00












  • Na(+)-translocating NADH-quinone reductase of marine and halophilic bacteria.

    abstract::The respiratory chain of marine and moderately halophilic bacteria requires Na+ for maximum activity, and the site of Na(+)-dependent activation is located in the NADH-quinone reductase segment. The Na(+)-dependent NADH-quinone reductase purified from marine bacterium Vibrio alginolyticus is composed of three subunits...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章,评审


    authors: Unemoto T,Hayashi M

    更新日期:1993-08-01 00:00:00

  • A glycine-rich sequence in the catalytic site of F-type ATPase.

    abstract::Affinity labeling and genetic studies on the glycine-rich sequence of the beta subunit of E. coli F-type ATPase are discussed. A model of the structure of the enzyme near the gamma phosphate moiety is proposed. ...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章,评审


    authors: Futai M,Iwamoto A,Omote H,Maeda M

    更新日期:1992-10-01 00:00:00

  • Plant uncoupling mitochondrial protein activity in mitochondria isolated from tomatoes at different stages of ripening.

    abstract::In the present study we have observed a higher state of coupling in respiring mitochondria isolated from green as compared to red tomatoes (Lycopersicon esculentum, Mill.). Green tomato mitochondria produced a membrane potential (deltapsi) high enough to phosphorylate ADP, whereas in red tomato mitochondria, BSA and A...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Costa AD,Nantes IL,Jezek P,Leite A,Arruda P,Vercesi AE

    更新日期:1999-10-01 00:00:00

  • New genes encoding subunits of a cytochrome bc1-analogous complex in the respiratory chain of the hyperthermoacidophilic crenarchaeon Sulfolobus acidocaldarius.

    abstract::The soxL gene from Sulfolobus acidocaldarius (DSM 639) encodes a Rieske iron-sulfur protein. In this study we report the identification of two open reading frames in its downstream region. The first one, named soxN, codes for a membrane protein bearing a resemblance to the b-type cytochromes of the cytochrome bc1 and ...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Hiller A,Henninger T,Schäfer G,Schmidt CL

    更新日期:2003-04-01 00:00:00

  • Control of ubiquinol oxidation at center P (Qo) of the cytochrome bc1 complex.

    abstract::The unique bifurcated oxidation of ubiquinol at center P (Qo) of the cytochrome bc1 complex is the reaction within the Q-cycle reaction scheme that is most critical for the link between electron transfer and vectorial proton translocation. While there is a general consensus about the overall reaction at center P, the ...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Brandt U

    更新日期:1999-06-01 00:00:00

  • Nonuniform lateral distribution of transmembrane deltapH in a coupling membrane as related to its curvature.

    abstract::The possibility is analyzed that the pH of the water space localized inside the invagination of a membrane can differ from the pH of the external bulk buffer outside the invagination. The proton flow responsible for decreased pH values inside mitochondrial cristae and membrane invaginations of cyanobacteria has been c...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Kara-Ivanov M

    更新日期:1983-06-01 00:00:00

  • Modification of respiratory-chain enzyme activities in brown adipose tissue mitochondria by idebenone (hydroxydecyl-ubiquinone).

    abstract::Idebenone (IDE), a synthetic analog of coenzyme Q, strongly activates glycerol phosphate (GP) oxidation in brown adipose tissue mitochondria. GP oxidase, GP cytochrome c oxidoreductase and GP dehydrogenase activities were all significantly stimulated by 13 muM IDE. Substituted derivatives of IDE acetyl- and methoxyide...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Rauchová H,Drahota Z,Bergamini C,Fato R,Lenaz G

    更新日期:2008-04-01 00:00:00

  • Electron redistribution in mixed valence cytochrome oxidase following photolysis of carboxy-oxidase.

    abstract::Absorbance changes at 446 nm in purified cytochrome oxidase following flash photolysis of carboxy-oxidase poised in the mixed valance state at +220 mV show biphasic kinetics. One phase corresponds to CO recombination to ferrous cytochrome a3 with an energy of activation of 9 kcal/mol; the second phase is 3-5 times fas...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Harmon HJ

    更新日期:1988-12-01 00:00:00

  • Purification of the channel component of the mitochondrial calcium uniporter and its reconstitution into planar lipid bilayers.

    abstract::The purification of the channel-forming component of the mitochondrial calcium uniporter and its channel properties are described. After ethanol and 50% ethanol-water extraction of mitochondria from beef heart or perfused rat liver, the extract was passed through thiopropyl-Sepharose 6B column, and absorbed components...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Mironova GD,Baumann M,Kolomytkin O,Krasichkova Z,Berdimuratov A,Sirota T,Virtanen I,Saris NE

    更新日期:1994-04-01 00:00:00

  • Short report: Tissue-specific expression profiles of the uncoupling protein family in normal control mice and genetically ob/ob mice.

    abstract::Uncoupling proteins (UCPs) located in the inner mitochondrial membrane are involved in the regulation of energy balance. Thus far, 5 UCP isoforms have been identified, but controversies exist in the research focused on the function of the UCPs (except UCP1) in the pathogenesis of obesity. Because of the known cross-re...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Zhang CM,Zhang M,Zhu JG,Ji CB,Zhu C,Kou CZ,Qin DN,Tong ML,Guo XR

    更新日期:2010-06-01 00:00:00

  • The structure and biochemistry of the vacuolar H+ ATPase in proximal and distal urinary acidification.

    abstract::Vacuolar H+ ATPases participate in renal hydrogen ion secretion in both the proximal and distal nephron. These plasma membrane forms of the vacuolar H+ ATPase are regulated physiologically to maintain the acid-base balance of the organism. Proton transporting renal cells have requirements for constitutive acidificatio...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章,评审


    authors: Gluck SL

    更新日期:1992-08-01 00:00:00

  • Role of intracellular pH in proliferation, transformation, and apoptosis.

    abstract::Both cellular proliferation and apoptosis (programmed cell death) have been claimed to be modulated, perhaps even triggered by, changes in intracellular pH. In this review, we summarize the evidence that gave rise to these hypotheses. To facilitate a critical appraisal of the existing data, we briefly review the main ...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章,评审


    authors: Shrode LD,Tapper H,Grinstein S

    更新日期:1997-08-01 00:00:00

  • The reconstitution of oxidative phosphorylation in mitochondria isolated from a ubiquinone-deficient mutant of Saccharomyces cerevisiae.

    abstract::Mitochondria, isolated from the ubiquinone-deficient nuclear mutant of Saccharomyces cerevisiae E3-24, are practically unable to oxidize exogenous substrates. Respiratory activity, coupled to ATP synthesis, can, however, be reconstituted by the simple addition of ethanolic solutions of ubiquinones. A minimal length of...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: De Santis A,Bertoli E,Di Gioia A,Melandri BA,Baccarini Melandri A

    更新日期:1982-06-01 00:00:00

  • A translational study "case report" on the small molecule "energy blocker" 3-bromopyruvate (3BP) as a potent anticancer agent: from bench side to bedside.

    abstract::The small alkylating molecule, 3-bromopyruvate (3BP), is a potent and specific anticancer agent. 3BP is different in its action from most currently available chemo-drugs. Thus, 3BP targets cancer cells' energy metabolism, both its high glycolysis ("Warburg Effect") and mitochondrial oxidative phosphorylation. This inh...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Ko YH,Verhoeven HA,Lee MJ,Corbin DJ,Vogl TJ,Pedersen PL

    更新日期:2012-02-01 00:00:00

  • Modulation of matrix Ca2+ content by the ADP/ATP carrier in brown adipose tissue mitochondria. Influence of membrane lipid composition.

    abstract::The role of the adenine nucleotide translocase on Ca2+ homeostasis in mitochondria from brown adipose tissue was examined. It was found that in mitochondria incubated with 50 microM Ca2+, ADP was not needed to retain the cation, but it was required for strengthening the inhibitory effect of cyclosporin on membrane per...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Chávez E,Moreno-Sánchez R,Torres-Marquez ME,Zazueta C,Bravo C,Rodríquez-Enríquez S,García C,Rodriguez JS,Martinez F

    更新日期:1996-02-01 00:00:00

  • Molecular defects of NADH-ubiquinone oxidoreductase (complex I) in mitochondrial diseases.

    abstract::Defects in Complex I of the mitochondrial respiratory chain have been identified in 38 patients. The clinical and laboratory features are reviewed and the results of recently devised strategies aimed at characterizing the primary molecular and genetic abnormalities are presented. Although not exhaustive, these studies...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章,评审


    authors: Morgan-Hughes JA,Schapira AH,Cooper JM,Clark JB

    更新日期:1988-06-01 00:00:00

  • Mutation of K234 and K236 in the voltage-dependent anion channel 1 impairs its insertion into the mitochondrial outer membrane.

    abstract::Previous in vitro studies indicated that mutation of both K234 and K236 to arginine, glutamine, or glutamic acid impaired the ability of the voltage-dependent anion channel (VDAC1) to insert into the outer membrane of the mitochondria (Smith et al. 1995). These same mutants were expressed in a strain of Saccharomyces ...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Angeles R,Devine J,Barton K,Smith M,McCauley R

    更新日期:1999-04-01 00:00:00

  • Electron microscopic structural analysis of Photosystem I, Photosystem II, and the cytochrome b6/f complex from green plants and cyanobacteria.

    abstract::Electron microscopy (EM) in combination with image analysis is a powerful technique to study protein structure at low- and high resolution. Since electron micrographs of biological objects are very noisy, substantial improvement of image quality can be obtained by averaging individual projections. Crystallographic and...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章,评审


    authors: Boekema EJ,Boonstra AF,Dekker JP,Rögner M

    更新日期:1994-02-01 00:00:00

  • The use of amphipathic maleimides to study membrane-associated proteins.

    abstract::A series of amphiphilic polymethlyenecarboxymaleimides has been synthesized for use as sulfhydryl reagents applicable to membrane proteins. Physical properties of the compounds which are relevant to their proposed mode of action have been determined. By comparing rates of reaction in aqueous and aprotic solvents, the ...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Griffiths DG,Partis MD,Churchill P,Brenner SC,Fleischer S,Moore RJ,Beechey RB

    更新日期:1990-10-01 00:00:00

  • The energy-conserving and energy-dissipating processes in mitochondria isolated from wild type and nonripening tomato fruits during development on the plant.

    abstract::Bioenergetics of tomato (Lycopersicon esculentum) development on the plant was followed from the early growing stage to senescence in wild type (climacteric) and nonripening mutant (nor, non-climacteric) fruits. Fruit development was expressed in terms of evolution of chlorophyll a content allowing the assessment of a...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Almeida AM,Navet R,Jarmuszkiewicz W,Vercesi AE,Sluse-Goffart CM,Sluse FE

    更新日期:2002-12-01 00:00:00

  • NM23/nucleoside diphosphate kinase and signal transduction.

    abstract::NM23s (or NDP kinases) regulate a fascinating variety of cellular activities, including proliferation, development, and differentiation. All these processes are modulated by external stimuli, leading to the idea that this family of proteins modulates transmembrane signaling pathways. This review summarizes the evidenc...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章,评审


    authors: Otero AS

    更新日期:2000-06-01 00:00:00

  • Oxygen metabolism and a potential role for cytochrome c oxidase in the Warburg effect.

    abstract::By manipulating the physical properties of oxygen, cells are able to harvest the large thermodynamic potential of oxidation to provide a substantial fraction of the energy necessary for cellular processes. The enzyme largely responsible for this oxygen manipulation is cytochrome c oxidase, which resides at the inner m...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章,评审


    authors: Herrmann PC,Herrmann EC

    更新日期:2007-06-01 00:00:00

  • Notch pathway activation mediated the senescence of endothelial progenitor cells in hypercholesterolemic mice.

    abstract::Hyperlipidemia is an important factor in the induction of cardiovascular diseases. However, the molecular mechanisms underlying the vascular injury involved in hyperlipidemia remains unclear. This study aimed to investigate the Notch pathway of endothelial progenitor cells (EPCs) in reendothelialization after vascular...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Liang J,Ke X,Yang R,Wang X,Du Z,Hu C

    更新日期:2020-12-01 00:00:00

  • Transformation with SV40 virus prevents retinoic acid inhibition of plasma membrane NADH diferric transferrin reductase in rat liver cells.

    abstract::Retinoic acid inhibits the reduction of diferric transferrin through the transplasma membrane electron transport system on fetal rat liver cells infected with a temperature-sensitive SV40 virus when the cells are in the nontransformed state cultured at 40 degrees C. When the cells are in the transformed state (grown a...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Sun IL,Toole-Simms W,Crane FL,Morré DJ,Löw H,Chou JY

    更新日期:1988-06-01 00:00:00

  • A unifying concept for ion translocation by retinal proteins.

    abstract::First, halorhodopsin is capable of pumping protons after illumination with green and blue light in the same direction as chloride. Second, mutated bacteriorhodopsin where the proton acceptor Asp85 and the proton donor Asp96 are replaced by Asn showed proton pump activity after illumination with blue light in the same ...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章,评审


    authors: Oesterhelt D,Tittor J,Bamberg E

    更新日期:1992-04-01 00:00:00

  • Neutral magnesium-dependent sphingomyelinase from liver plasma membrane: purification and inhibition by ubiquinol.

    abstract::Plasma membranes isolated from pig liver contained almost no acid sphingomyelinase but significant neutral magnesium-dependent sphingomyelinase that was activated by phosphatidylserine. We report here the purification to apparent homogeneity of neutral sphingomyelinase of about 87 kDa from liver plasma membranes. The ...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Martín SF,Navarro F,Forthoffer N,Navas P,Villalba JM

    更新日期:2001-04-01 00:00:00

  • External succinate and potassium ions influence Dcu dependent FOF1-ATPase activity and H+ flux of Escherichia coli at different pHs.

    abstract::During fermentation Escherichia coli transport succinate mainly via Dcu family carriers. Current paper describes the role of externally added succinate on N'N'-dicyclohexylcarbodiimide (DCCD) sensitive ATPase activity and H+ flux depending on potassium ions. At pH 7.5 in wild type membrane vesicles DCCD-sensitive ATPa...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Mikoyan G,Karapetyan L,Vassilian A,Trchounian A,Trchounian K

    更新日期:2020-10-01 00:00:00

  • Ionic channels in the plasma membrane of Schizosaccharomyces pombe: evidence from patch-clamp measurements.

    abstract::Patch-clamp studies of the yeast Schizosaccharomyces pombe reveal that the plasma membrane contains a voltage-gated channel mildly selective for potassium over sodium, lithium, and chloride. The channel exhibits several conductances with a maximum of 153 pS. The channel gates in the region of physiologically relevant ...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Vacata V,Höfer M,Larsson HP,Lecar H

    更新日期:1993-02-01 00:00:00

  • In vitro lipid oxidation modifies proteins and functional properties of sarcoplasmic reticulum.

    abstract::Changes in protein and fatty acid compositions of flounder sarcoplasmic reticulum during NADH plus ascorbate-dependent lipid peroxidation in vitro were related to the ability of the sarcoplasmic reticulum to sequester Ca+2. Progressive accumulation of high-molecular-weight protein components occurred concomitantly wit...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Luo SW,Hultin HO

    更新日期:1986-08-01 00:00:00

  • NYGGF4 (PID1) effects on insulin resistance are reversed by metformin in 3T3-L1 adipocytes.

    abstract::NYGGF4 (also called PID1) is a recently discovered gene that is involved in obesity-related insulin resistance (IR). We aimed in the present study to further elucidate the effects of NYGGF4 on IR and the underlying mechanisms through using metformin treatment in 3T3-L1 adipocytes. Our data showed that the metformin pr...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章


    authors: Qiu J,Wang YM,Shi CM,Yue HN,Qin ZY,Zhu GZ,Cao XG,Ji CB,Cui Y,Guo XR

    更新日期:2012-12-01 00:00:00