Abstract:
:Absorbance changes at 446 nm in purified cytochrome oxidase following flash photolysis of carboxy-oxidase poised in the mixed valance state at +220 mV show biphasic kinetics. One phase corresponds to CO recombination to ferrous cytochrome a3 with an energy of activation of 9 kcal/mol; the second phase is 3-5 times faster with an energy of activation of 9.15 kcal/mol. Following flash photolysis at approximately -60 degrees C, cytochromes a and c and the 840-nm CuA species are observed to undergo reduction as electrons from ferrous unliganded cytochrome a3 equilibrate with the equipotential redox centers of the oxidase; as CO recombines with ferrous cyochrome a3, these centers are oxidized and the mixed valence carboxy-oxidase is regenerated. Electron redistribution between centers of the oxidase in the forward and reverse directions occurs faster than does the binding of CO.
journal_name
J Bioenerg Biomembrjournal_title
Journal of bioenergetics and biomembranesauthors
Harmon HJdoi
10.1007/BF00762550subject
Has Abstractpub_date
1988-12-01 00:00:00pages
735-48issue
6eissn
0145-479Xissn
1573-6881journal_volume
20pub_type
杂志文章abstract::We report on the influence of dielectric pulse injection on the energy metabolism of yeast cells with a planar interdigitated electrode interface. The energy metabolism was measured via NADH fluorescence. The application of dielectric pulses results in a distinct decrease of the fluorescence, indicating a response of ...
journal_title:Journal of bioenergetics and biomembranes
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journal_title:Journal of bioenergetics and biomembranes
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journal_title:Journal of bioenergetics and biomembranes
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journal_title:Journal of bioenergetics and biomembranes
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journal_title:Journal of bioenergetics and biomembranes
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journal_title:Journal of bioenergetics and biomembranes
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journal_title:Journal of bioenergetics and biomembranes
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journal_title:Journal of bioenergetics and biomembranes
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journal_title:Journal of bioenergetics and biomembranes
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journal_title:Journal of bioenergetics and biomembranes
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journal_title:Journal of bioenergetics and biomembranes
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journal_title:Journal of bioenergetics and biomembranes
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abstract::One of the challenging features of energy-transducing terminal oxidases, like the aa3 cytochrome c oxidase of Paracoccus denitrificans, is the translocation of protons across the cytoplasmic membrane, which is coupled to the transfer of electrons to oxygen. As a prerequisite for a more advanced examination of the enzy...
journal_title:Journal of bioenergetics and biomembranes
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更新日期:1998-02-01 00:00:00
abstract::Idebenone (IDE), a synthetic analog of coenzyme Q, strongly activates glycerol phosphate (GP) oxidation in brown adipose tissue mitochondria. GP oxidase, GP cytochrome c oxidoreductase and GP dehydrogenase activities were all significantly stimulated by 13 muM IDE. Substituted derivatives of IDE acetyl- and methoxyide...
journal_title:Journal of bioenergetics and biomembranes
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更新日期:2008-04-01 00:00:00
abstract::The multidrug resistance (MDR) phenotype, frequently observed during cancer treatment, is often associated with drug efflux pump activity. However, many other factors are also known to be involved. Cancer cells often rely on aerobic glycolysis for energy production; this is known as the "Warburg effect" and is used as...
journal_title:Journal of bioenergetics and biomembranes
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更新日期:2013-10-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
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更新日期:1993-08-01 00:00:00
abstract::The midgut plasma membrane V-ATPase from larval Manduca sexta, the tobacco hornworm, is the sole energizer of any epithelial ion transport in this tissue and is responsible for the alkalinization of the gut lumen up to a pH of more than 11. This mini-review deals with those topics of research on this enzyme which may ...
journal_title:Journal of bioenergetics and biomembranes
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更新日期:1999-02-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/BF02110438
更新日期:1996-12-01 00:00:00
abstract::Mitochondria are responsible for many vital cellular functions in eukaryotic cells, such as ATP production, steroid synthesis and prosthetic group biogenesis. The vital functions of mitochondria are possible due to the compartmental nature of this organelle. Mitochondria form a dynamic network that can exist as a netw...
journal_title:Journal of bioenergetics and biomembranes
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更新日期:2017-02-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
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