In vitro lipid oxidation modifies proteins and functional properties of sarcoplasmic reticulum.

Abstract:

:Changes in protein and fatty acid compositions of flounder sarcoplasmic reticulum during NADH plus ascorbate-dependent lipid peroxidation in vitro were related to the ability of the sarcoplasmic reticulum to sequester Ca+2. Progressive accumulation of high-molecular-weight protein components occurred concomitantly with loss of Ca+2-sequestering activity. Part of this polymerized protein may be the dimer or trimer of Ca+2, Mg+2-ATPase. Loss in Ca+2, Mg+2-ATPase protein could account for over 60% of the polymerized protein. Rate of loss of polyunsaturated fatty acids was C22:6 greater than C20:4 greater than C20:5 greater than C22:5. Loss of polyunsaturated fatty acids and accumulation of thiobarbituric acid-reactive substances occurred concomitantly with protein polymerization.

journal_name

J Bioenerg Biomembr

authors

Luo SW,Hultin HO

doi

10.1007/BF00743052

subject

Has Abstract

pub_date

1986-08-01 00:00:00

pages

315-23

issue

4

eissn

0145-479X

issn

1573-6881

journal_volume

18

pub_type

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