Abstract:
:First, halorhodopsin is capable of pumping protons after illumination with green and blue light in the same direction as chloride. Second, mutated bacteriorhodopsin where the proton acceptor Asp85 and the proton donor Asp96 are replaced by Asn showed proton pump activity after illumination with blue light in the same direction as wildtype after green light illumination. These results can be explained by and are discussed in light of our new hypothesis: structural changes in either molecule lead to a change in ion affinity and accessibility for determining the vectoriality of the transport through the two proteins.
journal_name
J Bioenerg Biomembrjournal_title
Journal of bioenergetics and biomembranesauthors
Oesterhelt D,Tittor J,Bamberg Edoi
10.1007/BF00762676keywords:
subject
Has Abstractpub_date
1992-04-01 00:00:00pages
181-91issue
2eissn
0145-479Xissn
1573-6881journal_volume
24pub_type
杂志文章,评审abstract::Copper transporting P-type ATPases, designated ATP7A and ATP7B, play an essential role in mammalian copper balance. Impaired intestinal transport of copper, resulting from mutations in the ATP7A gene, lead to Menkes disease in humans. Defects in a similar gene, the copper transporting ATPase ATP7B, result in Wilson di...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1023/a:1021293818125
更新日期:2002-10-01 00:00:00
abstract::Electron microscopy (EM) in combination with image analysis is a powerful technique to study protein structure at low- and high resolution. Since electron micrographs of biological objects are very noisy, substantial improvement of image quality can be obtained by averaging individual projections. Crystallographic and...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1007/BF00763217
更新日期:1994-02-01 00:00:00
abstract::2-Arachidonoylglicerol (2-AG) is an endocannabinoid that mimics the pharmacological effects of Δ⁹ tetrahydrocannabinol, the psychoactive component of the plant Cannabis sativa. It is present in many mammalian tissues, such as brain, liver, spleen, heart and kidney, where it exerts different biological effects either r...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/s10863-012-9431-6
更新日期:2012-04-01 00:00:00
abstract::The electrolyte-pigment-metal system can be described as analogous to a photosensitive junction region. When the thickness of the pigment film is increased, the action spectra of the maximum short-circuit photo-current under continuous illumination differ from the absorption spectra both in direct illumination (pigmen...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/BF00750283
更新日期:1976-08-01 00:00:00
abstract::Complex I (NDH-1) translocates protons across the membrane using electron transfer energy. Two different coupling mechanisms are currently being discussed for complex I: direct (redox-driven) and indirect (conformation-driven). Semiquinone (SQ) intermediates are suggested to be key for the coupling mechanism. Recently...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1007/s10863-014-9557-9
更新日期:2014-08-01 00:00:00
abstract::The mitochondrial UPR (UPRmt) is rapidly gaining attention. While most studies on the UPRmt have focused on its role in aging, emerging studies suggest an important role of the UPRmt in cancer. Further, several of the players of the UPRmt in mammalian cells have well reported roles in the maintenance of the organelle....
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1007/s10863-017-9722-z
更新日期:2017-08-01 00:00:00
abstract::The Nm23-H1/nucleoside diphosphate (NDP) kinase A is a metastasis suppressor, besides its enzymatic activity. The mutant S120G has been found in high-grade neuroblastomas. The mutant protein, once denatured in urea, is unable to refold in vitro. A size-exclusion chromatography analysis of the folding/association pathw...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/s10863-006-9042-1
更新日期:2006-08-01 00:00:00
abstract::We have previously shown that vacuolar H+-ATPase subcomplex V(o) from mung bean contains subunit d, however, its sequence and function were unknown. In the present study, we report the cloning and recombinant over expression of subunit d from mung bean in E. coli. To study the function of subunit d, two vacuolar H+-AT...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/s10863-008-9193-3
更新日期:2008-12-01 00:00:00
abstract::Retinoic acid inhibits the reduction of diferric transferrin through the transplasma membrane electron transport system on fetal rat liver cells infected with a temperature-sensitive SV40 virus when the cells are in the nontransformed state cultured at 40 degrees C. When the cells are in the transformed state (grown a...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/BF00769639
更新日期:1988-06-01 00:00:00
abstract::This study was designed to investigate the energetics of isolated rat hepatocyte swelling due to sodium-cotransported amino acid accumulation in a medium containing either glucose or octanoate as basal substrate. We show that the size of the increase in cytosolic volume is directly correlated with the total amino acid...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1023/a:1022487119390
更新日期:1997-12-01 00:00:00
abstract::Hepatic iron toxicity because of iron overload seems to be mediated by lipid peroxidation of biological membranes and the associated organelle dysfunctions. However, the basic mechanisms underlying this process in vivo are still little understood. Gerbils were dosed with weekly injections of iron-dextran alone or in c...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1023/a:1005512014280
更新日期:2000-04-01 00:00:00
abstract::Succinate-driven reverse electron transport (RET) is one of the main sources of mitochondrial reactive oxygen species (mtROS) in ischemia-reperfusion injury. RET is dependent on mitochondrial membrane potential (Δψm) and transmembrane pH difference (ΔpH), components of the proton motive force (pmf); a decrease in Δψm ...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/s10863-018-9766-8
更新日期:2018-10-01 00:00:00
abstract::Recent advances in structural biology underlying mechanisms of channel gating have strengthened our knowledge about how K(+) channels can be inter-convertible between conductive and non-conductive states. We have reviewed and combined mutagenesis with biochemical, biophysical and structural information in order to und...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1007/s10863-012-9407-6
更新日期:2012-02-01 00:00:00
abstract::Renal artery clamping itself induces renal ischemia which subsequently causes renal cell injury and can lead to renal failure. The duration of warm ischemia that would be safe for postoperative kidney function during partial nephrectomy remains under investigations. Mitochondria play an important role in pathophysiolo...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/s10863-016-9643-2
更新日期:2016-02-01 00:00:00
abstract::We report on the influence of dielectric pulse injection on the energy metabolism of yeast cells with a planar interdigitated electrode interface. The energy metabolism was measured via NADH fluorescence. The application of dielectric pulses results in a distinct decrease of the fluorescence, indicating a response of ...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/s10863-006-9014-5
更新日期:2006-04-01 00:00:00
abstract::Electron transport from Photosystem II to Photosystem I of spinach chloroplasts can be stimulated by bicarbonate and various carbonyl or carboxyl compounds. Monovalent or divalent cations, which have hitherto been implicated in the energy distribution between the two photosystems, i.e., "spillover" phenomena at low li...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/BF00744683
更新日期:1980-08-01 00:00:00
abstract::Plasma membrane vesicles of HeLa cells are characterized by a drug-responsive oxidation of NADH. The NADH oxidation takes place in an argon or nitrogen atmosphere and in samples purged of oxygen. Direct assay of protein thiols by reaction with 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB; Ellman's reagent), suggests tha...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1023/a:1020594214379
更新日期:1998-10-01 00:00:00
abstract::Mitochondria are dynamic structures for which fusion and fission are well characterized for rapidly dividing cells in culture. Based on these data, it has recently been proposed that high respiratory activity is the result of fusion and formation of mitochondrial reticulum, while fission results in fragmented mitochon...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1007/s10863-012-9494-4
更新日期:2013-08-01 00:00:00
abstract::The oxidation of NADH by mouse liver plasma membranes was shown to be accompanied by the formation of H2O2. The rate of H2O2 formation was less than one-tenth the rate of oxygen uptake and much slower than the rate of reduction of artificial electron acceptors. The optimum pH for this reaction was 7.0 and the Km value...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/BF00743203
更新日期:1981-12-01 00:00:00
abstract::In Propionigenium modestum, ATP is manufactured from ADP and phosphate by the enzyme ATP synthase using the free energy of an electrochemical gradient of Na+ ions. The P. modestum ATP synthase is a clear member of the family of F-type ATP synthases and the only major distinction is an extension of the coupling ion spe...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1023/a:1005608823087
更新日期:2000-10-01 00:00:00
abstract::A sodium-dependent phosphate transporter (type II Na/Pi-cotransporter) was isolated which is expressed in apical membranes of proximal tubules and exhibits transport characteristics similar as described for renal reabsorption of phosphate. Type II associated Na/Pi-cotransport is electrogenic and results obtained by el...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1023/a:1020525409894
更新日期:1998-04-01 00:00:00
abstract::Members of the genus Methanosarcina are strictly anaerobic archaea that derive their metabolic energy from the conversion of a restricted number of substrates to methane. H2 + CO2 are converted to CH4 via the CO2-reducing pathway, while methanol and methylamines are metabolized by the methylotrophic pathway. Two novel...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1023/b:jobb.0000019598.64642.97
更新日期:2004-02-01 00:00:00
abstract::Liver plasma membranes isolated from rats with chronic dietary iron overload showed a large modification of their phospholipid fatty acid composition. Specifically, a significant decrease in polyunsaturated fatty acids and a parallel increase in saturated fatty acids was observed. This pattern was consistent with the ...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/BF00762524
更新日期:1989-08-01 00:00:00
abstract::This introductory article and those that follow focus on the roles that mitochondria may have in cancer metastasis (spreading) that all too frequently leads to death of cancer patients. The history of cancer dates back in time to several thousand years BC and continues to this day. Although billions of dollars have be...
journal_title:Journal of bioenergetics and biomembranes
pub_type:
doi:10.1007/s10863-012-9470-z
更新日期:2012-12-01 00:00:00
abstract::Traditionally, mitochondria have been viewed as the "powerhouse" of the cell, i.e., the site of the oxidative phosphorylation machinery involved in ATP production. Consequently, much of the research conducted on mitochondria over the past 4 decades has focused on elucidating both those molecular events involved in ATP...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1023/a:1005453700533
更新日期:1999-08-01 00:00:00
abstract::Phospholysine phosphohistidine inorganic pyrophosphate phosphatase (LHPP) has been recently identified as a novel inhibitor of multiple tumors; however, its role in glioblastoma (GBM) has not been investigated. This study aimed to evaluate whether LHPP exerts a potential tumor-inhibiting role in GBM. Compared with tha...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/s10863-020-09866-0
更新日期:2021-02-01 00:00:00
abstract::The P515 absorbance change upon single-turnover light flashes has been studied in intact leaves and isolated chloroplasts from spinach. A comparative study of the effects of preillumination on the kinetics of the P515 response and on the activity of the chloroplast ATPase has been made. The slow component (reaction 2)...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/BF00751054
更新日期:1983-12-01 00:00:00
abstract::Arsenic trioxide is an effective treatment for acute promyelocytic leukemia, but resistance to metalloid salts is found in humans. Using atomic absorption spectroscopy, we have measured the rate of uptake of arsenic trioxide and of antimony tartrate in GLC4 and GLC4/ADR cells overexpressing MRP1 and the rate of their ...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1023/a:1015180026665
更新日期:2002-04-01 00:00:00
abstract::Transformation of 3T3 cells by SV40 virus changes the properties of the transplasma membrane electron transport activity which can be assayed by reduction of external ferric salts. After 42 h of culture and before the growth rate is maximum, the transformed cells have a much slower rate of ferric reduction. The change...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/BF00786008
更新日期:1991-12-01 00:00:00
abstract::Defects in Complex I of the mitochondrial respiratory chain have been identified in 38 patients. The clinical and laboratory features are reviewed and the results of recently devised strategies aimed at characterizing the primary molecular and genetic abnormalities are presented. Although not exhaustive, these studies...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1007/BF00769638
更新日期:1988-06-01 00:00:00