Abstract:
:Beef heart cytochrome c oxidase was reconstituted in asolectin liposomes containing the pH indicator fluorescein-phosphatidylethanolamine (FPE) by the cholate-dialysis procedure. The influence of FPE on the asolectin liposome size and of the removal of subunit III from the complex on its incorporation into liposomes was analyzed by freeze-fracture electron microscopy. Samples were frozen without the addition of cryoprotectants. The vesicle size distribution of native enzyme reconstituted into asolectin liposomes was homogeneous, 84% of the population having a diameter of 14-37 +/- 7.5 mm. The preparation containing FPE had a similar vesicle size distribution, but with bigger diameter range (20-50 nm). In all three different types of proteoliposome preparations the majority of particles containing vesicles was found to have 1 particle (42-81%). The absence of subunit III did not influence the incorporation of the enzyme into the liposomes and was as good as the preparation with native enzyme (greater than 99%). Therefore we conclude that the suppression of the proton pump activity was due to the intrinsic properties of subunit III and not to defective incorporation into artificial membrane systems.
journal_name
J Bioenerg Biomembrjournal_title
Journal of bioenergetics and biomembranesauthors
Müller M,Azzi Adoi
10.1007/BF00743111subject
Has Abstractpub_date
1985-12-01 00:00:00pages
385-93issue
6eissn
0145-479Xissn
1573-6881journal_volume
17pub_type
杂志文章abstract::The development of new anti-neoplastic drugs is a key issue for cancer chemotherapy due to the reality that, most likely, certain cancer cells are resistant to current chemotherapy. The past two decades have witnessed tremendous advances in our understanding of the pathogenesis of cancer. These advances have allowed i...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/s10863-012-9489-1
更新日期:2013-02-01 00:00:00
abstract::Forkhead box protein O6 (FOXO6) has been recently identified as a novel regulator of oxidative stress in multiple pathological processes. However, whether FOXO6 participates in the regulation of oxidative stress of myocardial infarction is unclear. The present study was performed to evaluate the potential role of FOXO...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/s10863-020-09856-2
更新日期:2020-12-01 00:00:00
abstract::The sarcoplasmic reticulum (SR) of skeletal muscle controls the contraction-relaxation cycle by raising and lowering the myoplasmic free-Ca2+ concentration. The coupling between excitation, i.e., depolarization of sarcolemma and transverse tubule (TT) and Ca2+ release from the terminal cisternae (TC) of SR takes place...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1007/BF00812069
更新日期:1989-04-01 00:00:00
abstract::Results are presented which, taken with evidence developed by others, suggest a general mechanism for the entry and binding of exogenous ligands (including O2) at the "binuclear site" (CuBFea3) of the heme-copper oxidases. The mechanism includes a "ligand shuttle" wherein the obligatory way station for incoming ligand...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1007/BF00762859
更新日期:1993-04-01 00:00:00
abstract::Yeasts capable of growing and surviving at high temperatures are regarded as thermotolerant. For appropriate functioning of cellular processes and cell survival, the maintenance of an optimal redox state is critical of reducing and oxidizing species. We studied mitochondrial functions of the thermotolerant Kluyveromyc...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/s10863-017-9696-x
更新日期:2017-04-01 00:00:00
abstract::Renal artery clamping itself induces renal ischemia which subsequently causes renal cell injury and can lead to renal failure. The duration of warm ischemia that would be safe for postoperative kidney function during partial nephrectomy remains under investigations. Mitochondria play an important role in pathophysiolo...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/s10863-016-9643-2
更新日期:2016-02-01 00:00:00
abstract::Cellular Ca2+ signals are crucial in the control of most physiological processes, cell injury and programmed cell death; mitochondria play a pivotal role in the regulation of such cytosolic Ca2+ ([Ca2+]c) signals. Mitochondria are endowed with multiple Ca2+ transport mechanisms by which they take up and release Ca2+ a...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1023/a:1005508311495
更新日期:2000-02-01 00:00:00
abstract::First, halorhodopsin is capable of pumping protons after illumination with green and blue light in the same direction as chloride. Second, mutated bacteriorhodopsin where the proton acceptor Asp85 and the proton donor Asp96 are replaced by Asn showed proton pump activity after illumination with blue light in the same ...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1007/BF00762676
更新日期:1992-04-01 00:00:00
abstract::The ATP synthetase of chloroplast membranes binds ADP and ATP with high affinity, and the binding becomes quasi-irreversible under certain conditions. One explanation of the function of these nucleotides is that they are transiently tightly bound during ATP synthesis as part of the catalytic process, and that the rele...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/BF00744524
更新日期:1983-10-01 00:00:00
abstract::Previous in vitro studies indicated that mutation of both K234 and K236 to arginine, glutamine, or glutamic acid impaired the ability of the voltage-dependent anion channel (VDAC1) to insert into the outer membrane of the mitochondria (Smith et al. 1995). These same mutants were expressed in a strain of Saccharomyces ...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1023/a:1005451811802
更新日期:1999-04-01 00:00:00
abstract::Cytoplasmic male sterility arises when mitochondrial activities are disrupted that are essential for pollen development. Rearrangements in the mitochondrial genome that create novel open reading frames are strongly correlated with CMS phenotypes in a number of systems. The morphological aberrations which indicate CMS-...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1007/BF02110007
更新日期:1995-08-01 00:00:00
abstract::We have previously shown that vacuolar H+-ATPase subcomplex V(o) from mung bean contains subunit d, however, its sequence and function were unknown. In the present study, we report the cloning and recombinant over expression of subunit d from mung bean in E. coli. To study the function of subunit d, two vacuolar H+-AT...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/s10863-008-9193-3
更新日期:2008-12-01 00:00:00
abstract::Dysfunction of neuronal metabolism is central to all forms of seizure activity. Clinical, genetic and metabolic studies have implicated mitochondria in the process. Alterations in electron transport, generation of reactive oxygen species, calcium metabolism and apoptosis all have been described in human and animal ner...
journal_title:Journal of bioenergetics and biomembranes
pub_type:
doi:10.1007/s10863-010-9322-7
更新日期:2010-12-01 00:00:00
abstract::The serotonergic system plays a crucial role in the energy balance regulation. Energy balance is mediated by food intake and caloric expenditure. Thus, the present study investigated the mechanisms that might be associated with fluoxetine treatment-induced weight reduction. Wistar male rat pups received daily injectio...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/s10863-015-9617-9
更新日期:2015-08-01 00:00:00
abstract::Since the nineteenth century, a great amount of different biological structures and processes have been assessed by fluorescent dyes. Along with the uses of these compounds as vital and histological dyes, some fluorescent dyes have become valuable tools for the study of the pore phenomenon in plasma membranes. Some io...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/s10863-015-9613-0
更新日期:2015-08-01 00:00:00
abstract::Reconstitution experiments were performed on lipid bilayer membranes in the presence of purified mitochondrial porin from yeast and of detergent-solubilized mitochondrial outer membranes of a porin-free yeast mutant. The addition of the porin resulted in a strong increase of the membrane conductance, which was caused ...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1007/BF00762516
更新日期:1989-08-01 00:00:00
abstract::The Nm23-H1/nucleoside diphosphate (NDP) kinase A is a metastasis suppressor, besides its enzymatic activity. The mutant S120G has been found in high-grade neuroblastomas. The mutant protein, once denatured in urea, is unable to refold in vitro. A size-exclusion chromatography analysis of the folding/association pathw...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/s10863-006-9042-1
更新日期:2006-08-01 00:00:00
abstract::The soxL gene from Sulfolobus acidocaldarius (DSM 639) encodes a Rieske iron-sulfur protein. In this study we report the identification of two open reading frames in its downstream region. The first one, named soxN, codes for a membrane protein bearing a resemblance to the b-type cytochromes of the cytochrome bc1 and ...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1023/a:1023742002493
更新日期:2003-04-01 00:00:00
abstract::In the mitochondrial F(O)F(1) ATP-synthase/ATPase complex, subunits α and β are part of the extrinsic portion that catalyses ATP synthesis. Since there are no reports about genes and proteins from these subunits in crustaceans, we analyzed the cDNA sequences of both subunits in the whiteleg shrimp Litopenaeus vannamei...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/s10863-011-9340-0
更新日期:2011-04-01 00:00:00
abstract::P-glycoprotein (Pgp), the ATP-binding cassette (ABC) transporter, confers multidrug resistance to cancer cells by extruding cytotoxic natural product amphipathic drugs using the energy of ATP hydrolysis. Our studies are directed toward understanding the mechanism of action of Pgp and recent work deals with the assessm...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1023/a:1012875105006
更新日期:2001-12-01 00:00:00
abstract::A series of amphiphilic polymethlyenecarboxymaleimides has been synthesized for use as sulfhydryl reagents applicable to membrane proteins. Physical properties of the compounds which are relevant to their proposed mode of action have been determined. By comparing rates of reaction in aqueous and aprotic solvents, the ...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/BF00809072
更新日期:1990-10-01 00:00:00
abstract::Mitochondria are dynamic structures for which fusion and fission are well characterized for rapidly dividing cells in culture. Based on these data, it has recently been proposed that high respiratory activity is the result of fusion and formation of mitochondrial reticulum, while fission results in fragmented mitochon...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1007/s10863-012-9494-4
更新日期:2013-08-01 00:00:00
abstract::Many oxidoreductases are constructed from (a) local sites of strongly coupled substrate-redox cofactor partners participating in exchange of electron pairs, (b) electron pair/single electron transducing redox centers, and (c) nonadiabatic, long-distance, single-electron tunneling between weakly coupled redox centers. ...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1007/BF02110096
更新日期:1995-06-01 00:00:00
abstract::The importance of crucial nutrient factors like Phosphate (P) and their limited availability leads to variable fluctuations in fatty acid and phospholipid synthesis in the green alga. These fatty acids and phospholipids are an imperative byproduct of alga which used in biofuel production. The production of phospholipi...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/s10863-019-09813-8
更新日期:2020-02-01 00:00:00
abstract::Heat shock protein 90 (Hsp90) is an attractive therapeutic target. Geldanamycin (GA), the first identified Hsp90 inhibitor, exhibited potent antitumor activity, but possessed significant hepatotoxicity. To overcome the hepatotoxicity derived from the quinone structure of GA, a non-quinone GA derivative 17-demethoxy-re...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/s10863-015-9620-1
更新日期:2015-10-01 00:00:00
abstract::A rapid loss of accumulated Ca2+ is produced by addition of H+ to isolated heart mitochondria. The H+-dependent Ca+ efflux requires that either (a) the NAD(P)H pool of the mitochondrion be oxidized, or (b) the endogenous adenine nucleotides be depleted. The loss of Ca2+ is accompanied by swelling and loss of endogenou...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/BF00743069
更新日期:1982-12-01 00:00:00
abstract::The present state of the chemiosmotic concept is reviewed. Special attention is paid to (i) further progress in studies on the Na(+)-coupled energetics and (ii) paradoxical bioenergetic effects when protonic or sodium potentials are utilized outside the coupling membrane (TonB-mediated uphill transports across the out...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1007/BF00831533
更新日期:1994-12-01 00:00:00
abstract::F1-ATPases transiently entrap inhibitory MgADP in a catalytic site during turnover when noncatalytic sites are not saturated with ATP. An initial burst of ATP hydrolysis rapidly decelerates to a slow intermediate rate that gradually accelerates to a final steady-state rate. Transition from the intermediate to the fina...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1007/BF02113985
更新日期:1996-10-01 00:00:00
abstract::Ferrochelatase [heme synthase, protoheme ferrolyase (EC 4.99.1.1)], the terminal enzyme of the heme biosynthetic pathway, catalyzes the incorporation of ferrous ion into protoporphyrin IX to form protoheme IX. The genes and cDNAs for ferrochelatase from mammals and micro-organisms have been isolated. The gene for huma...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1007/BF02110038
更新日期:1995-04-01 00:00:00
abstract::Retinoic acid inhibits the reduction of diferric transferrin through the transplasma membrane electron transport system on fetal rat liver cells infected with a temperature-sensitive SV40 virus when the cells are in the nontransformed state cultured at 40 degrees C. When the cells are in the transformed state (grown a...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/BF00769639
更新日期:1988-06-01 00:00:00