Plant uncoupling mitochondrial protein activity in mitochondria isolated from tomatoes at different stages of ripening.

Abstract:

:In the present study we have observed a higher state of coupling in respiring mitochondria isolated from green as compared to red tomatoes (Lycopersicon esculentum, Mill.). Green tomato mitochondria produced a membrane potential (deltapsi) high enough to phosphorylate ADP, whereas in red tomato mitochondria, BSA and ATP were required to restore deltapsi to the level of that obtained with green tomato mitochondria. This supports the notion that such uncoupling in red tomato mitochondria is mediated by a plant uncoupling mitochondrial protein (PUMP; cf. Vercesi et al., 1995). Nevertheless, mitochondria from both green and red tomatoes exhibited an ATP-sensitive linoleic acid (LA)-induced deltapsi decrease providing evidence that PUMP is also present in green tomatoes. Indeed, proteoliposomes containing reconstituted green or red tomato PUMP showed LA uniport and LA-induced H+ transport. It is suggested that the higher concentration of free fatty acids (PUMP substrates) in red tomatoes could explain the lower coupling state in mitochondria isolated from these fruits.

journal_name

J Bioenerg Biomembr

authors

Costa AD,Nantes IL,Jezek P,Leite A,Arruda P,Vercesi AE

doi

10.1023/a:1005408809619

keywords:

subject

Has Abstract

pub_date

1999-10-01 00:00:00

pages

527-33

issue

5

eissn

0145-479X

issn

1573-6881

journal_volume

31

pub_type

杂志文章
  • Mutational analysis of assembly and function of the iron-sulfur protein of the cytochrome bc1 complex in Saccharomyces cerevisiae.

    abstract::The iron-sulfur protein of the cytochrome bc1 complex oxidizes ubiquinol at center P in the protonmotive Q cycle mechanism, transferring one electron to cytochrome c1 and generating a low-potential ubisemiquinone anion which reduces the low-potential cytochrome b-566 heme group. In order to catalyze this divergent tra...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章,评审

    doi:10.1007/BF00762586

    authors: Graham LA,Brandt U,Sargent JS,Trumpower BL

    更新日期:1993-06-01 00:00:00

  • Crystal structure of bovine heart cytochrome c oxidase at 2.8 A resolution.

    abstract::Thirteen different polypeptide subunits, each in one copy, five phosphatidyl ethanolamines and three phosphatidyl glycerols, two hemes A, three Cu ions, one Mg ion, and one Zn ion are detectable in the crystal structure of bovine heart cytochrome c oxidase in the fully oxidized form at 2.8 A resolution. A propionate o...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章,评审

    doi:10.1023/a:1020595108560

    authors: Yoshikawa S,Shinzawa-Itoh K,Tsukihara T

    更新日期:1998-02-01 00:00:00

  • Sulfite inhibits the F1F0-ATP synthase and activates the F1F0-ATPase of Paracoccus denitrificans.

    abstract::The F1F0 complex of Paracoccus denitrificans (PdF1F0) is the fastest ATP synthase but the slowest ATPase. Sulfite exerts maximal activation of the PdF1F0-ATPase (Pacheco-Moisés, F., García, J. J., Rodríguez-Zavala, J. S., and Moreno-Sánchez, R. (2000). Eur J. Biochem. 267, 993-1000) but its effect on the PdF1F0-ATP sy...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章

    doi:10.1023/a:1020252401675

    authors: Pacheco-Moisés F,Minauro-Sanmiguel F,Bravo C,García JJ

    更新日期:2002-08-01 00:00:00

  • The sulfonylurea-inhibited NADH oxidase activity of HeLa cell plasma membranes has properties of a protein disulfide-thiol oxidoreductase with protein disulfide-thiol interchange activity.

    abstract::Plasma membrane vesicles of HeLa cells are characterized by a drug-responsive oxidation of NADH. The NADH oxidation takes place in an argon or nitrogen atmosphere and in samples purged of oxygen. Direct assay of protein thiols by reaction with 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB; Ellman's reagent), suggests tha...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章

    doi:10.1023/a:1020594214379

    authors: Morré DJ,Chueh PJ,Lawler J,Morré DM

    更新日期:1998-10-01 00:00:00

  • Neuronal apoptosis: BH3-only proteins the real killers?

    abstract::At present there is a poor understanding of the events that lead up to neuronal apoptosis that occurs in neurodegenerative diseases and following acute ischemic episodes. Apoptosis is critical for the elimination of unwanted neurons within the developing nervous system. The Bcl-2 family of proteins contains pro- and a...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章,评审

    doi:10.1023/B:JOBB.0000041756.23918.11

    authors: Ward MW,Kögel D,Prehn JH

    更新日期:2004-08-01 00:00:00

  • Nuclear magnetic resonance methods to characterize lipid-protein interactions at membrane surfaces.

    abstract::Specific molecular interactions that determine many of the functions of a biomembrane have a high probability of occurring at the surface of that membrane. However, unlike their hydrophobic core, the polar-apolar interface of biomembranes has been somewhat neglected experimentally. Reasons for this are that the chemic...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章,评审

    doi:10.1007/BF00762300

    authors: Watts A

    更新日期:1987-12-01 00:00:00

  • Calcium binding to the subunit c of E. coli ATP-synthase and possible functional implications in energy coupling.

    abstract::The 8-kDa subunit c of the E. coli F0 ATP-synthase proton channel was tested for Ca++ binding activity using a 45Ca++ ligand blot assay after transferring the protein from SDS-PAGE gels onto polyvinyl difluoride membranes. The purified subunit c binds 45Ca++ strongly with Ca++ binding properties very similar to those ...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章

    doi:10.1007/BF02110438

    authors: Zakharov SD,Li X,Red'ko TP,Dilley RA

    更新日期:1996-12-01 00:00:00

  • Membrane potential and delta pH dependency of reverse electron transport-associated hydrogen peroxide production in brain and heart mitochondria.

    abstract::Succinate-driven reverse electron transport (RET) is one of the main sources of mitochondrial reactive oxygen species (mtROS) in ischemia-reperfusion injury. RET is dependent on mitochondrial membrane potential (Δψm) and transmembrane pH difference (ΔpH), components of the proton motive force (pmf); a decrease in Δψm ...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章

    doi:10.1007/s10863-018-9766-8

    authors: Komlódi T,Geibl FF,Sassani M,Ambrus A,Tretter L

    更新日期:2018-10-01 00:00:00

  • A novel plasma membrane quinone reductase and NAD(P)H:quinone oxidoreductase 1 are upregulated by serum withdrawal in human promyelocytic HL-60 cells.

    abstract::We have studied changes in plasma membrane NAD(P)H:quinone oxidoreductases of HL-60 cells under serum withdrawal conditions, as a model to analyze cell responses to oxidative stress. Highly enriched plasma membrane fractions were obtained from cell homogenates. A major part of NADH-quinone oxidoreductase in the plasma...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章

    doi:10.1023/a:1016035504049

    authors: Forthoffer N,Gómez-Díaz C,Bello RI,Burón MI,Martín SF,Rodríguez-Aguilera JC,Navas P,Villalba JM

    更新日期:2002-06-01 00:00:00

  • Physical link and functional coupling of presynaptic calcium channels and the synaptic vesicle docking/fusion machinery.

    abstract::N- and P/Q-type calcium channels are localized in high density in presynaptic nerve terminals and are crucial elements in neuronal excitation-secretion coupling. In addition to mediating Ca2+ entry to initiate transmitter release, they are thought to interact directly with proteins of the synaptic vesicle docking/fusi...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章,评审

    doi:10.1023/a:1021985521748

    authors: Sheng ZH,Westenbroek RE,Catterall WA

    更新日期:1998-08-01 00:00:00

  • LHPP exerts a tumor-inhibiting role in glioblastoma via the downregulation of Akt and Wnt/β-catenin signaling.

    abstract::Phospholysine phosphohistidine inorganic pyrophosphate phosphatase (LHPP) has been recently identified as a novel inhibitor of multiple tumors; however, its role in glioblastoma (GBM) has not been investigated. This study aimed to evaluate whether LHPP exerts a potential tumor-inhibiting role in GBM. Compared with tha...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章

    doi:10.1007/s10863-020-09866-0

    authors: Li C,Yang J,Wang W,Li R

    更新日期:2021-02-01 00:00:00

  • Simple oxygraphic analysis for the presence of adenylate kinase 1 and 2 in normal and tumor cells.

    abstract::The adenylate kinase (AK) isoforms network plays an important role in the intracellular energy transfer processes, the maintenance of energy homeostasis, and it is a major player in AMP metabolic signaling circuits in some highly-differentiated cells. For this purpose, a rapid and sensitive method was developed that e...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章

    doi:10.1007/s10863-016-9687-3

    authors: Klepinin A,Ounpuu L,Guzun R,Chekulayev V,Timohhina N,Tepp K,Shevchuk I,Schlattner U,Kaambre T

    更新日期:2016-10-01 00:00:00

  • Lipid composition and fluidity of liver plasma membranes from rats with chronic dietary iron overload.

    abstract::Liver plasma membranes isolated from rats with chronic dietary iron overload showed a large modification of their phospholipid fatty acid composition. Specifically, a significant decrease in polyunsaturated fatty acids and a parallel increase in saturated fatty acids was observed. This pattern was consistent with the ...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章

    doi:10.1007/BF00762524

    authors: Pietrangelo A,Tripodi A,Carulli N,Tomasi A,Ceccarelli D,Ventura E,Masini A

    更新日期:1989-08-01 00:00:00

  • Ion transport in liver mitochondria from normal and thyroxine-treated rats.

    abstract::Liver mitochondria isolated from rats 24 h after a single subcutaneous injection of 8 mg thyroxine per kilogram body weight were compared with those isolated from control rats that received injections of isotonic saline at the same time. The mitochondria isolated from the thyroxine-treated rats show higher rates of en...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章

    doi:10.1007/BF00748766

    authors: Shears SB,Bronk JR

    更新日期:1980-12-01 00:00:00

  • Sodium, protons, and energy coupling in the methanogenic bacteria.

    abstract::In this review, I focus on the bioenergetics of the methanogenic bacteria, with particular attention directed to the roles of transmembrane electrochemical gradients of sodium and proton. In addition, the mechanism of coupling ATP synthesis to methanogenic electron transfer is addressed. Evidence is reviewed which sug...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章,评审

    doi:10.1007/BF00762689

    authors: Lancaster JR Jr

    更新日期:1989-12-01 00:00:00

  • The unraveling architecture of the junctional sarcoplasmic reticulum.

    abstract::The sarcoplasmic reticulum (SR) of skeletal muscle controls the contraction-relaxation cycle by raising and lowering the myoplasmic free-Ca2+ concentration. The coupling between excitation, i.e., depolarization of sarcolemma and transverse tubule (TT) and Ca2+ release from the terminal cisternae (TC) of SR takes place...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章,评审

    doi:10.1007/BF00812069

    authors: Volpe P

    更新日期:1989-04-01 00:00:00

  • An integrated molecular modeling approach for the tryptase monomer-curcuminoid recognition analysis: conformational and bioenergetic features.

    abstract::Human mast cell tryptase has been shown as an activating enzyme in matrix degradation process. The previous study suggest that tryptase either alone or in joining with activation of metalloproteinases, can associate in extra cellular matrix damage and the possible destruction of the basement membrane resulting in phot...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章

    doi:10.1007/s10863-018-9777-5

    authors: Wongrattanakamon P,Ampasavate C,Sirithunyalug B,Jiranusornkul S

    更新日期:2018-12-01 00:00:00

  • Electron redistribution in mixed valence cytochrome oxidase following photolysis of carboxy-oxidase.

    abstract::Absorbance changes at 446 nm in purified cytochrome oxidase following flash photolysis of carboxy-oxidase poised in the mixed valance state at +220 mV show biphasic kinetics. One phase corresponds to CO recombination to ferrous cytochrome a3 with an energy of activation of 9 kcal/mol; the second phase is 3-5 times fas...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章

    doi:10.1007/BF00762550

    authors: Harmon HJ

    更新日期:1988-12-01 00:00:00

  • The mechanism of action of multidrug-resistance-linked P-glycoprotein.

    abstract::P-glycoprotein (Pgp), the ATP-binding cassette (ABC) transporter, confers multidrug resistance to cancer cells by extruding cytotoxic natural product amphipathic drugs using the energy of ATP hydrolysis. Our studies are directed toward understanding the mechanism of action of Pgp and recent work deals with the assessm...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章,评审

    doi:10.1023/a:1012875105006

    authors: Sauna ZE,Smith MM,Müller M,Kerr KM,Ambudkar SV

    更新日期:2001-12-01 00:00:00

  • Evaluation by steady-state enzyme kinetics of the role of tightly bound nucleotides during photophosphorylation.

    abstract::The ATP synthetase of chloroplast membranes binds ADP and ATP with high affinity, and the binding becomes quasi-irreversible under certain conditions. One explanation of the function of these nucleotides is that they are transiently tightly bound during ATP synthesis as part of the catalytic process, and that the rele...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章

    doi:10.1007/BF00744524

    authors: Tiefert MA,Shavit N

    更新日期:1983-10-01 00:00:00

  • Modification of transplasma membrane oxidoreduction by SV40 transformation of 3T3 cells.

    abstract::Transformation of 3T3 cells by SV40 virus changes the properties of the transplasma membrane electron transport activity which can be assayed by reduction of external ferric salts. After 42 h of culture and before the growth rate is maximum, the transformed cells have a much slower rate of ferric reduction. The change...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章

    doi:10.1007/BF00786008

    authors: Löw H,Crane FL,Grebing C,Isaksson M,Lindgren A,Sun IL

    更新日期:1991-12-01 00:00:00

  • Transferring knowledge towards understanding the pore stabilizing variations in K(+) channels: pore stability in K(+) channels.

    abstract::Recent advances in structural biology underlying mechanisms of channel gating have strengthened our knowledge about how K(+) channels can be inter-convertible between conductive and non-conductive states. We have reviewed and combined mutagenesis with biochemical, biophysical and structural information in order to und...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章,评审

    doi:10.1007/s10863-012-9407-6

    authors: Raja M,Olrichs NK,Vales E,Schrempf H

    更新日期:2012-02-01 00:00:00

  • Energetics of isolated hepatocyte swelling induced by sodium co-transported amino acids.

    abstract::This study was designed to investigate the energetics of isolated rat hepatocyte swelling due to sodium-cotransported amino acid accumulation in a medium containing either glucose or octanoate as basal substrate. We show that the size of the increase in cytosolic volume is directly correlated with the total amino acid...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章

    doi:10.1023/a:1022487119390

    authors: Espié P,Devin A,Guérin B,Rigoulet M

    更新日期:1997-12-01 00:00:00

  • Mutation of K234 and K236 in the voltage-dependent anion channel 1 impairs its insertion into the mitochondrial outer membrane.

    abstract::Previous in vitro studies indicated that mutation of both K234 and K236 to arginine, glutamine, or glutamic acid impaired the ability of the voltage-dependent anion channel (VDAC1) to insert into the outer membrane of the mitochondria (Smith et al. 1995). These same mutants were expressed in a strain of Saccharomyces ...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章

    doi:10.1023/a:1005451811802

    authors: Angeles R,Devine J,Barton K,Smith M,McCauley R

    更新日期:1999-04-01 00:00:00

  • Dependence on the F0F1-ATP synthase for the activities of the hydrogen-oxidizing hydrogenases 1 and 2 during glucose and glycerol fermentation at high and low pH in Escherichia coli.

    abstract::Escherichia coli has four [NiFe]-hydrogenases (Hyd); three of these, Hyd-1, Hyd-2 and Hyd-3 have been characterized well. In this study the requirement for the F(0)F(1)-ATP synthase for the activities of the hydrogen-oxidizing hydrogenases Hyd-1 and Hyd-2 was examined. During fermentative growth on glucose at pH 7.5 a...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章

    doi:10.1007/s10863-011-9397-9

    authors: Trchounian K,Pinske C,Sawers RG,Trchounian A

    更新日期:2011-12-01 00:00:00

  • Structure, function and regulation of the tricarboxylate transport protein from rat liver mitochondria.

    abstract::Recent progress is summarized on the structure, function, and regulation of the tricarboxylate (i.e., citrate) transport protein (CTP) from the rat liver mitochondrial inner membrane. The transporter has been purified and its reconstituted function characterized. A cDNA clone encoding the CTP has been isolated and seq...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章

    doi:10.1007/BF01108407

    authors: Kaplan RS,Mayor JA

    更新日期:1993-10-01 00:00:00

  • How do alterations in plant mitochondrial genomes disrupt pollen development?

    abstract::Cytoplasmic male sterility arises when mitochondrial activities are disrupted that are essential for pollen development. Rearrangements in the mitochondrial genome that create novel open reading frames are strongly correlated with CMS phenotypes in a number of systems. The morphological aberrations which indicate CMS-...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章,评审

    doi:10.1007/BF02110007

    authors: Conley CA,Hanson MR

    更新日期:1995-08-01 00:00:00

  • Molecular properties of the fungal plasma-membrane [H+]-ATPase.

    abstract::The fungal plasma membrane contains a proton-translocating ATPase that is closely related, both structurally and functionally, to the [Na+, K+]-, [H+, K+]-, and [Ca2+]-ATPases of animal cells, the plasma-membrane [H+]-ATPase of higher plants, and several bacterial cation-transporting ATPases. This review summarizes cu...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章,评审

    doi:10.1007/BF00808117

    authors: Nakamoto RK,Slayman CW

    更新日期:1989-10-01 00:00:00

  • Monoclonal antibodies to the Ca2+ + Mg2+-dependent ATPase of sarcoplasmic reticulum identify polymorphic forms of the enzyme and indicate the presence in the enzyme of a classical high-affinity Ca2+ binding site.

    abstract::In order to determine whether polymorphic forms of the Ca2+ + Mg2+-dependent ATPase exist, we have examined the cross-reactivity of five monoclonal antibodies prepared against the rabbit skeletal muscle sarcoplasmic reticulum enzyme with proteins from microsomal fractions isolated from a variety of muscle and nonmuscl...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章

    doi:10.1007/BF00743238

    authors: Zubrzycka-Gaarn E,MacDonald G,Phillips L,Jorgensen AO,MacLennan DH

    更新日期:1984-12-01 00:00:00

  • Pores from mitochondrial outer membranes of yeast and a porin-deficient yeast mutant: a comparison.

    abstract::Reconstitution experiments were performed on lipid bilayer membranes in the presence of purified mitochondrial porin from yeast and of detergent-solubilized mitochondrial outer membranes of a porin-free yeast mutant. The addition of the porin resulted in a strong increase of the membrane conductance, which was caused ...

    journal_title:Journal of bioenergetics and biomembranes

    pub_type: 杂志文章,评审

    doi:10.1007/BF00762516

    authors: Benz R,Schmid A,Dihanich M

    更新日期:1989-08-01 00:00:00