Abstract:
:The ATP synthetase of chloroplast membranes binds ADP and ATP with high affinity, and the binding becomes quasi-irreversible under certain conditions. One explanation of the function of these nucleotides is that they are transiently tightly bound during ATP synthesis as part of the catalytic process, and that the release of tightly bound ATP from one catalytic site is promoted when ADP and P(i) bind to a second catalytic site on the enzyme. Alternatively, it is possible that the tightly bound nucleotides are not catalytic, but instead have some regulatory function. We developed steady-state rate equations for both these models for photophosphorylation and tested them with experiments where two alternative substrates, ADP and GDP, were phosphorylated simultaneously. It was impossible to fit the results to the equations that assumed a catalytic role for tightly bound nucleotides, whether we assumed that both ADP and GDP, or only ADP, are phosphorylated by a mechanism involving substrate-induced release of product from another catalytic site. On the other hand, the equations derived from the regulatory-site model that we tested were able to fit all the results relatively well and in an internally consistent manner. We therefore conclude that the tightly bound nucleotides most likely do not derive from catalytic intermediates of ATP synthesis, but that substrate (and possibly also product) probably bind both to catalytic sites and to noncatalytic sites. The latter may modulate the transition of the ATP-synthesizing enzyme complex between its active and inactive states.
journal_name
J Bioenerg Biomembrjournal_title
Journal of bioenergetics and biomembranesauthors
Tiefert MA,Shavit Ndoi
10.1007/BF00744524subject
Has Abstractpub_date
1983-10-01 00:00:00pages
257-76issue
5eissn
0145-479Xissn
1573-6881journal_volume
15pub_type
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journal_title:Journal of bioenergetics and biomembranes
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journal_title:Journal of bioenergetics and biomembranes
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journal_title:Journal of bioenergetics and biomembranes
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journal_title:Journal of bioenergetics and biomembranes
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journal_title:Journal of bioenergetics and biomembranes
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journal_title:Journal of bioenergetics and biomembranes
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更新日期:2006-02-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
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更新日期:1998-04-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
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abstract::Recent studies of isolated muscle membrane have enabled induction and monitoring of rapid Ca2+ release from sarcoplasmic reticulum (SR)5 in vitro by a variety of methods. On the other hand, various proteins that may be directly or indirectly involved in the Ca2+ release mechanism have begun to be unveiled. In this min...
journal_title:Journal of bioenergetics and biomembranes
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journal_title:Journal of bioenergetics and biomembranes
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更新日期:2001-06-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1007/s10863-005-9487-7
更新日期:2005-12-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
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更新日期:1984-08-01 00:00:00
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pub_type: 杂志文章,评审
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journal_title:Journal of bioenergetics and biomembranes
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journal_title:Journal of bioenergetics and biomembranes
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
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更新日期:1999-08-01 00:00:00
abstract::Complete initial steady state kinetics of NADH-decylubiquinone (DQ) oxidoreductase reaction between pH 6.5 and 9.0 show an ordered sequential mechanism in which the order of substrate bindings and product releases is NADH-DQ-DQH2-NAD+. NADH binding to the free enzyme is accelerated by protonation of an amino acid (pos...
journal_title:Journal of bioenergetics and biomembranes
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更新日期:2003-10-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/BF00768540
更新日期:1987-08-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
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更新日期:1986-08-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章,评审
doi:10.1007/BF02110699
更新日期:1996-06-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
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更新日期:1986-08-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
doi:10.1007/s10863-012-9442-3
更新日期:2012-06-01 00:00:00
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journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
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更新日期:2020-02-01 00:00:00
abstract::The topology of beef heart Complex III has been studied by tryptic and chymotryptic digestion of isolated Complex III, Mg2+-ATP submitochondrial particles, and mitoplasts. Degradation products were detected by the immunoreplication technique using specific antibodies against core protein 1 (50 K) and core protein 2 (4...
journal_title:Journal of bioenergetics and biomembranes
pub_type: 杂志文章
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更新日期:1983-02-01 00:00:00