Fluorescent dyes as a reliable tool in P2X7 receptor-associated pore studies.

abstract：:N- and P/Q-type calcium channels are localized in high density in presynaptic nerve terminals and are crucial elements in neuronal excitation-secretion coupling. In addition to mediating Ca2+ entry to initiate transmitter release, they are thought to interact directly with proteins of the synaptic vesicle docking/fusi...

journal_title：Journal of bioenergetics and biomembranes

authors： Sheng ZH,Westenbroek RE,Catterall WA

更新日期：1998-08-01 00:00:00

abstract：:Members of the genus Methanosarcina are strictly anaerobic archaea that derive their metabolic energy from the conversion of a restricted number of substrates to methane. H2 + CO2 are converted to CH4 via the CO2-reducing pathway, while methanol and methylamines are metabolized by the methylotrophic pathway. Two novel...

journal_title：Journal of bioenergetics and biomembranes

authors： Deppenmeier U

更新日期：2004-02-01 00:00:00

abstract：:Three classes of inner mitochondrial membrane (IMM) channel activities have been defined by direct measurement of conductance levels in membranes with patch clamp techniques in 150 mM KCl. The "107 pS activity" is slightly anion selective and voltage dependent (open with matrix positive potentials). "Multiple conducta...

journal_title：Journal of bioenergetics and biomembranes

authors： Kinnally KW,Antonenko YN,Zorov DB

更新日期：1992-02-01 00:00:00

abstract：:We investigated the effects of K(ATP) channel openers diazoxide and pinacidil on the respiration rate and membrane potential (deltapsi) of rat heart mitochondria, oxidizing pyruvate and malate. Diazoxide and pinacidil (58.8-1348.3 microM) increased the V2 (-ADP) respiration rate accordingly by 13-208% and 30-273% and ...

journal_title：Journal of bioenergetics and biomembranes

authors： Kopustinskiene DM,Jovaisiene J,Liobikas J,Toleikis A

更新日期：2002-02-01 00:00:00

abstract：:Epilepsy is considered one of the most common neurological disorders worldwide. The burst firing neurons associated with prolonged epileptic discharges could lead to a large number of changes with events of cascades at the cellular level. From its role as the cellular powerhouse, mitochondria also play a crucial role ...

journal_title：Journal of bioenergetics and biomembranes

authors： Chen SD,Chang AY,Chuang YC

更新日期：2010-12-01 00:00:00

abstract：:High-conductance calcium-activated potassium (maxi-K) channels comprise a specialized family of K+ channels. They are unique in their dual requirement for depolarization and Ca2+ binding for transition to the open, or conducting, state. Ion conduction through maxi-K channels is blocked by a family of venom-derived pep...

journal_title：Journal of bioenergetics and biomembranes

authors： Kaczorowski GJ,Knaus HG,Leonard RJ,McManus OB,Garcia ML

更新日期：1996-06-01 00:00:00

abstract：:We summarize our current view of the reaction mechanism in F(1)-ATPase as it has emerged from experiment, theory, and computational studies over the last several years. ATP catalysis in the catalytic binding pockets of F(1) takes place without the release of any significant free energy and is efficiently driven by the...

journal_title：Journal of bioenergetics and biomembranes

authors： Dittrich M,Schulten K

更新日期：2005-12-01 00:00:00

abstract：:Changes in protein and fatty acid compositions of flounder sarcoplasmic reticulum during NADH plus ascorbate-dependent lipid peroxidation in vitro were related to the ability of the sarcoplasmic reticulum to sequester Ca+2. Progressive accumulation of high-molecular-weight protein components occurred concomitantly wit...

journal_title：Journal of bioenergetics and biomembranes

authors： Luo SW,Hultin HO

更新日期：1986-08-01 00:00:00

abstract：:P-glycoprotein (Pgp), the ATP-binding cassette (ABC) transporter, confers multidrug resistance to cancer cells by extruding cytotoxic natural product amphipathic drugs using the energy of ATP hydrolysis. Our studies are directed toward understanding the mechanism of action of Pgp and recent work deals with the assessm...

journal_title：Journal of bioenergetics and biomembranes

authors： Sauna ZE,Smith MM,Müller M,Kerr KM,Ambudkar SV

更新日期：2001-12-01 00:00:00

abstract：:Plasma membranes isolated from pig liver contained almost no acid sphingomyelinase but significant neutral magnesium-dependent sphingomyelinase that was activated by phosphatidylserine. We report here the purification to apparent homogeneity of neutral sphingomyelinase of about 87 kDa from liver plasma membranes. The ...

journal_title：Journal of bioenergetics and biomembranes

authors： Martín SF,Navarro F,Forthoffer N,Navas P,Villalba JM

更新日期：2001-04-01 00:00:00

abstract：:The proton/hydroxide (H+/OH-) permeability of phospholipid bilayer membranes at neutral pH is at least five orders of magnitude higher than the alkali or halide ion permeability, but the mechanism(s) of H+/OH- transport are unknown. This review describes the characteristics of H+/OH- permeability and conductance throu...

journal_title：Journal of bioenergetics and biomembranes

authors： Gutknecht J

更新日期：1987-10-01 00:00:00

abstract：:Free radical production and leak of brain nonsynaptic mitochondria were higher with pyruvate/malate than with succinate in rats and pigeons. Rotenone, antimycin A, and myxothiazol maximally stimulated free radical production with pyruvate/malate but not with succinate. Simultaneous treatment with myxothiazol plus anti...

journal_title：Journal of bioenergetics and biomembranes

authors： Barja G,Herrero A

更新日期：1998-06-01 00:00:00

abstract：:Complete initial steady state kinetics of NADH-decylubiquinone (DQ) oxidoreductase reaction between pH 6.5 and 9.0 show an ordered sequential mechanism in which the order of substrate bindings and product releases is NADH-DQ-DQH2-NAD+. NADH binding to the free enzyme is accelerated by protonation of an amino acid (pos...

journal_title：Journal of bioenergetics and biomembranes

authors： Hano N,Nakashima Y,Shinzawa-Itoh K,Terada H,Yoshikawa S

更新日期：2003-10-01 00:00:00

abstract：:The ATP-binding cassette (ABC) family of membrane transport proteins is the largest class of transporters in humans (48 members). The majority of ABC transporters function at the cell surface. Therefore, defective folding and trafficking of the protein to the cell surface can lead to serious health problems. The class...

journal_title：Journal of bioenergetics and biomembranes

authors： Loo TW,Bartlett MC,Clarke DM

更新日期：2005-12-01 00:00:00

abstract：:In this review, I focus on the bioenergetics of the methanogenic bacteria, with particular attention directed to the roles of transmembrane electrochemical gradients of sodium and proton. In addition, the mechanism of coupling ATP synthesis to methanogenic electron transfer is addressed. Evidence is reviewed which sug...

journal_title：Journal of bioenergetics and biomembranes

authors： Lancaster JR Jr

更新日期：1989-12-01 00:00:00

abstract：:The Mg2+ dependent asymmetry of the F(1)-ATPase catalytic sites leads to the differences in affinity for nucleotides and is an essential component of the binding-change mechanism. Changes in metal ligands during the catalytic cycle responsible for this asymmetry were characterized by vanadyl (V(IV) + O)2+, a functiona...

journal_title：Journal of bioenergetics and biomembranes

authors： Frasch WD

更新日期：2000-10-01 00:00:00

abstract：:In the present study we have observed a higher state of coupling in respiring mitochondria isolated from green as compared to red tomatoes (Lycopersicon esculentum, Mill.). Green tomato mitochondria produced a membrane potential (deltapsi) high enough to phosphorylate ADP, whereas in red tomato mitochondria, BSA and A...

journal_title：Journal of bioenergetics and biomembranes

authors： Costa AD,Nantes IL,Jezek P,Leite A,Arruda P,Vercesi AE

更新日期：1999-10-01 00:00:00

abstract：:A great deal of progress has been made in understanding both the structure and the mechanism of F1-ATPase. The primary structure is now fully known for at least five species. Sequence comparison between chloroplast, photobacteria, aerobic bacteria, and mitochondrial representatives allow us to infer more general funct...

journal_title：Journal of bioenergetics and biomembranes

authors： Ysern X,Amzel LM,Pedersen PL

更新日期：1988-08-01 00:00:00

abstract：:Oxygen plays a key role in bacterial bioluminescence. The simultaneous and continuous kinetics of oxygen consumption and light emission during a complete exhaustion of the exogenous oxygen present in a closed system has been investigated. The kinetics are performed with Vibrio fischeri, V. harveyi, and Photobacterium ...

journal_title：Journal of bioenergetics and biomembranes

authors： Bourgois JJ,Sluse FE,Baguet F,Mallefet J

更新日期：2001-08-01 00:00:00

abstract：:We report on the influence of dielectric pulse injection on the energy metabolism of yeast cells with a planar interdigitated electrode interface. The energy metabolism was measured via NADH fluorescence. The application of dielectric pulses results in a distinct decrease of the fluorescence, indicating a response of ...

journal_title：Journal of bioenergetics and biomembranes

authors： Reiher A,Warnke C,Radoch S,Witte H,Krtschil A,Mair T,Müller SC,Krost A

更新日期：2006-04-01 00:00:00

abstract：:Mitochondrial impairment has been collecting more and more attention as a contributing factor to the etiology of Parkinson's disease. Above all, the NADH-quinone oxidoreductase, complex I, of the respiratory chain seems to be most culpable. Complex I dysfunction is translated to an increased production of reactive oxy...

journal_title：Journal of bioenergetics and biomembranes

authors： Marella M,Seo BB,Yagi T,Matsuno-Yagi A

更新日期：2009-12-01 00:00:00

abstract：:Caloric restriction (CR) is widely known to increase life span and resistance to different types of injuries in several organisms. We have previously shown that mitochondria from livers or brains of CR animals exhibit higher calcium uptake rates and lower sensitivity to calcium-induced mitochondrial permeability trans...

journal_title：Journal of bioenergetics and biomembranes

authors： Serna JDC,Caldeira da Silva CC,Kowaltowski AJ

更新日期：2020-08-01 00:00:00

abstract：:In the mitochondrial F(O)F(1) ATP-synthase/ATPase complex, subunits α and β are part of the extrinsic portion that catalyses ATP synthesis. Since there are no reports about genes and proteins from these subunits in crustaceans, we analyzed the cDNA sequences of both subunits in the whiteleg shrimp Litopenaeus vannamei...

journal_title：Journal of bioenergetics and biomembranes

authors： Martinez-Cruz O,Garcia-Carreño F,Robles-Romo A,Varela-Romero A,Muhlia-Almazan A

更新日期：2011-04-01 00:00:00

abstract：:The bacterial phosphoenolpyruvate-dependent carbohydrate phosphotransferase system (PTS) consists of several proteins whose primary functions are to transport and phosphorylate their substrates. The complexity of the PTS undoubtedly reflects its additional roles in chemotaxis to PTS substrates and in regulation of oth...

journal_title：Journal of bioenergetics and biomembranes

authors： Jacobson GR,Saraceni-Richards C

更新日期：1993-12-01 00:00:00

abstract：:The purpose of this study was to characterize the physical properties of cytochrome c oxidase from rat liver. The enzyme was extracted from isolated mitochondria with nonionic detergents and further purified by ion-exchange chromatography on DEAE Bio-Gel A. The purified enzyme contained 9.64 nmol heme a/mg protein and...

journal_title：Journal of bioenergetics and biomembranes

authors： Ades IZ,Cascarano J

更新日期：1977-08-01 00:00:00

abstract：:Defects in Complex I of the mitochondrial respiratory chain have been identified in 38 patients. The clinical and laboratory features are reviewed and the results of recently devised strategies aimed at characterizing the primary molecular and genetic abnormalities are presented. Although not exhaustive, these studies...

journal_title：Journal of bioenergetics and biomembranes

authors： Morgan-Hughes JA,Schapira AH,Cooper JM,Clark JB

更新日期：1988-06-01 00:00:00

abstract：:2-Arachidonoylglicerol (2-AG) is an endocannabinoid that mimics the pharmacological effects of Δ⁹ tetrahydrocannabinol, the psychoactive component of the plant Cannabis sativa. It is present in many mammalian tissues, such as brain, liver, spleen, heart and kidney, where it exerts different biological effects either r...

journal_title：Journal of bioenergetics and biomembranes

authors： Zaccagnino P,D'Oria S,Romano LL,Di Venere A,Sardanelli AM,Lorusso M

更新日期：2012-04-01 00:00:00

abstract：:Succinate-driven reverse electron transport (RET) is one of the main sources of mitochondrial reactive oxygen species (mtROS) in ischemia-reperfusion injury. RET is dependent on mitochondrial membrane potential (Δψm) and transmembrane pH difference (ΔpH), components of the proton motive force (pmf); a decrease in Δψm ...

journal_title：Journal of bioenergetics and biomembranes

authors： Komlódi T,Geibl FF,Sassani M,Ambrus A,Tretter L

更新日期：2018-10-01 00:00:00

abstract：:The 95 kDa subunit a of eukaryotic V-ATPases consists of a C-terminal, ion-translocating part and an N-terminal cytosolic domain. The latter's N-terminal domain (~40 kDa) is described to bind in an acidification-dependent manner with cytohesin-2 (ARNO), giving the V-ATPase the putative function as pH-sensing receptor....

journal_title：Journal of bioenergetics and biomembranes

authors： Dip PV,Saw WG,Roessle M,Marshansky V,Grüber G

更新日期：2012-06-01 00:00:00

abstract：:Calorie restriction (CR) has been shown to decrease H(2)O(2) production in liver mitochondria, although it is not known if this is due to uniform changes in all mitochondria or changes in particular mitochondrial sub-populations. To address this issue, liver mitochondria from control and CR mice were fractionated usin...

journal_title：Journal of bioenergetics and biomembranes

authors： Hagopian K,Chen Y,Simmons Domer K,Soo Hoo R,Bentley T,McDonald RB,Ramsey JJ

更新日期：2011-06-01 00:00:00