Abstract:
:Different odorant-binding proteins (OBPs) were isolated from total antennal homogenates of male and female Bombyx mori. Proteins were separated according to their isoelectric point by using preparative fast-flow isoelectrofocusing. Odorant-binding proteins were identified in immunoblots by antisera raised against the pheromone-binding protein (anti-PBP) and the general odorant-binding protein (anti-GOBP2) of Antheraea polyphemus. Four proteins cross-reacting with anti-PBP were detected in males and two in females, while three proteins cross-reacting with anti-GOBP2 were found in males and five in females. Both anti-PBP and anti-GOBP2 cross-reacting proteins had an apparent molecular weight of 15-16 kDa. In parallel, the same two antisera were used in immunocytochemical studies in order to determine the distribution of these proteins within the various subtypes of olfactory sensilla. The presence of multiple odorant-binding proteins within one moth species as well as their complex distribution pattern support the suggestion that soluble OBPs might have a function in odorant discrimination.
journal_name
Chem Sensesjournal_title
Chemical sensesauthors
Maida R,Proebstl T,Laue Mdoi
10.1093/chemse/22.5.503subject
Has Abstractpub_date
1997-10-01 00:00:00pages
503-15issue
5eissn
0379-864Xissn
1464-3553journal_volume
22pub_type
杂志文章相关文献
CHEMICAL SENSES文献大全abstract::The cabbage moth, Mamestra brassicae L. (Lepidoptera, Noctuidae), is a polyphagous species that is often choosing plants of Brassica as hosts for oviposition. In the search for biologically relevant odorants used by these moths, gas chromatography linked to electrophysiological recordings from single receptor neurons ...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/bjm061
更新日期:2008-01-01 00:00:00
abstract::The effects of Triton X-100 on the olfactory epithelium (OE) of adult zebrafish were examined to study neuronal turnover in this model system. Fish were killed at various time points after detergent application and stained with hematoxylin and eosin to examine olfactory structures, immunocytochemistry to examine cell ...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/bjq019
更新日期:2010-06-01 00:00:00
abstract::Evidence suggests that women outperform men in core aspects of odor perception, and sex hormones may play a significant role in moderating this effect. The gender-affirming treatment (GAT) of transgender persons constitutes a powerful natural experiment to study the psychological and behavioral effects of high dosages...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/bjz069
更新日期:2020-01-01 00:00:00
abstract::In order to explore the cross-modal cognitive associations between smell and taste, a chemosensory analogue of the Stroop task (Stroop 1935) was developed. Fourteen participants were presented with an odorant and a tastant and asked to identify the tastant as "sweet" or "sour" by pressing 1 of 2 buttons as quickly as ...
journal_title:Chemical senses
pub_type: 杂志文章,随机对照试验
doi:10.1093/chemse/bjm001
更新日期:2007-05-01 00:00:00
abstract::Nasal inhalation is the basis of olfactory perception and drives neural activity in olfactory and limbic brain regions. Therefore, our ability to investigate the neural underpinnings of olfaction and respiration can only be as good as our ability to characterize features of respiratory behavior. However, recordings of...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/bjy045
更新日期:2018-09-22 00:00:00
abstract::To examine the functional subdivision of the teleost olfactory bulb, extracellular recordings were made from the posterior part of the medial region of the olfactory bulb in the crucian carp, Carassius carassius. Bulbar units classified as type I or type II were frequently and simultaneously encountered at a recording...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/28.3.181
更新日期:2003-03-01 00:00:00
abstract::Single carbon to 18 carbon n-aliphatic carboxylic acids were tested for their attractive effects on female Aedes aegypti in a Y-tube olfactometer. Each acid was tested over a wide range of concentrations together with L-(+)-lactic acid, the indispensable synergist for other attractive components emitted from human hos...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/oxfordjournals.chemse.a014042
更新日期:2000-06-01 00:00:00
abstract::Anosmia and hyposmia, the inability or decreased ability to smell, is estimated to afflict 3-20% of the population. Risk of olfactory dysfunction increases with old age and may also result from chronic sinonasal diseases, severe head trauma, and upper respiratory infections, or neurodegenerative diseases. These disord...
journal_title:Chemical senses
pub_type: 杂志文章,评审
doi:10.1093/chemse/bjx025
更新日期:2017-09-01 00:00:00
abstract::Cigarettes are an often-used consumer product, and flavor is an important determinant of their product appeal. Cigarettes with strong nontobacco flavors are popular among young people, and may facilitate smoking initiation. Discriminating flavors in tobacco is important for regulation purposes, for instance to set upp...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/bjw123
更新日期:2017-03-01 00:00:00
abstract::In 20 normal and four anosmic participants, instantaneous inhalation and exhalation flow rates were recorded in response to 15 s stimulations with clean air or propionic acid concentrations (0.16, 1.14, 8.22 and 59.15 p.p.m., v/v) that ranged from peri-threshold for normals to clearly supra-threshold for anosmics. Eac...
journal_title:Chemical senses
pub_type: 临床试验,杂志文章
doi:10.1093/chemse/26.4.351
更新日期:2001-05-01 00:00:00
abstract::In chum salmon captured at the coastal sea and the natal river, the magnitudes of the olfactory nerve responses to the amino acids after perfusion of the olfactory epithelium with artificial pond water (APW) were similar to those after perfusion with artificial sea water (ASW), although the concentrations of Na+, Cl- ...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/19.6.609
更新日期:1994-12-01 00:00:00
abstract::The vast number of detectable odors makes matching olfactory receptors (ORs) to their ligands a daunting task. Krautwurst and colleagues have hypothesized that this process can be simplified by focusing on those odorants that are perceptually relevant food odors. In this issue of Chemical Senses, they use this framewo...
journal_title:Chemical senses
pub_type: 评论,杂志文章
doi:10.1093/chemse/bjx002
更新日期:2017-03-01 00:00:00
abstract::The central projections of olfactory receptor cells associated with two distinct types of antennal sensilla in the sphinx moth Manduca sexta were revealed by anterograde staining. In both sexes, receptor axons that arise from sexually isomorphic, type-II trichoid sensilla (and possibly some basiconic sensilla) project...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/20.3.313
更新日期:1995-06-01 00:00:00
abstract::The suitability of Beidler's mixture equation for mixtures of sucrose and maltitol as well as for mixtures of sucrose and aspartame was examined in the presence of an orange aroma. The mean scores for the attribute sweet remained constant for each combination of sucrose and maltitol and for each combination of sucrose...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/23.1.59
更新日期:1998-02-01 00:00:00
abstract::In order to study potential mixture interactions among bitter compounds, selected sodium salts were added to five compounds presented either alone or as binary bitter-compound mixtures. Each compound was tested at a concentration that elicited 'weak' perceived bitterness. The bitter compounds were mixed at these conce...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/bjh045
更新日期:2004-06-01 00:00:00
abstract::Clinically, olfaction can fail in any of three ways: (i) decreased sensitivity (hyposmia, anosmia) and two types of distortion (dysosmia); (ii) distorted quality of an odorant stimulation (troposmia); (iii) perceived odor when no odorant is present (phantosmia, hallucination). The distortions are usually much more ups...
journal_title:Chemical senses
pub_type: 杂志文章,评审
doi:10.1093/chemse/27.7.611
更新日期:2002-09-01 00:00:00
abstract::The present study demonstrated that 100 microM amiloride serves as an ineffective conditioned taste stimulus in a taste aversion paradigm. Even if amiloride has a detectable taste, it's unlikely that its behavioral effects in salt mixture experiments are due to its inherent taste quality. ...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/20.5.559
更新日期:1995-10-01 00:00:00
abstract::Taste sensitivity is assessed with various techniques, including absolute detection and quality recognition. For any stimulus, one might expect individual differences in sensitivity to be reflected in all measures, but they are often surprisingly independent. Here, we focus on sensitivity to sour and salty taste, in p...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/bjt003
更新日期:2013-05-01 00:00:00
abstract::Experiments were performed to determine which bundles of the olfactory tracts were essential for mediating alarm reaction in crucian carp (Carassius carassius L.). The fish were maintained in physiological saline after surgery to preserve the remaining tracts and postoperative inspections revealed the functionality of...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/25.1.103
更新日期:2000-02-01 00:00:00
abstract::Odorants and flavorants typically contain many components. It is generally easier to detect multicomponent stimuli than to detect a single component, through either neural integration or probability summation (PS) (or both). PS assumes that the sensory effects of 2 (or more) stimulus components (e.g., gustatory and ol...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/bjr103
更新日期:2012-03-01 00:00:00
abstract::The perceived bitterness intensity for bitter solutions of propylthiouracil (PROP), sucrose octa-acetate (SOA), quinine HCl and caffeine were examined in a genetically informative sample of 392 females and 313 males (mean age of 17.8 +/- 3.1 years), including 62 monozygotic and 131 dizygotic twin pairs and 237 sib pai...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/bjj044
更新日期:2006-06-01 00:00:00
abstract::There is no natural physical continuum for odor quality along which an odor quality discrimination (OQD) threshold can be measured. In an attempt to overcome this problem, the substitution-reciprocity (SURE) method defines a framework for the measurement of an OQD threshold. More specifically, it (i) defines a thresho...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/25.5.493
更新日期:2000-10-01 00:00:00
abstract::The vomeronasal organ (VNO) is important for activating accessory olfactory pathways that are involved in sexually dimorphic mating behavior. The VNO of male garter snakes is critically important for detection of, and response to, female sex pheromones. In the present study, under voltage-clamp conditions, male snake ...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/bjj056
更新日期:2006-07-01 00:00:00
abstract::Mosquito control is essential to protect humans from mosquito-borne diseases. The host recognition between mosquitoes and humans is achieved by the mosquito olfactory system. Antennal sensilla, which house olfactory receptor neurons, are responsible for detecting chemical cues from hosts. To deepen our understanding o...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/bjv089
更新日期:2016-06-01 00:00:00
abstract::Previous studies have reported that the umami taste of monosodium l-glutamate (MSG) and salty-smelling odors (e.g., soy sauce, bacon, sardines) enhance the perception of saltiness. This study aimed to investigate the neural basis of the enhancement of saltiness in human participants using functional near-infrared spec...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/bjx084
更新日期:2018-02-26 00:00:00
abstract::Our study was designed to examine how components of complex mixtures can inhibit the binding of other components to receptor sites in the olfactory system of the spiny lobster Panulirus argus. Biochemical binding assays were used to study how two- to six-component mixtures inhibit binding of the radiolabeled odorants ...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/23.3.269
更新日期:1998-06-01 00:00:00
abstract::Since the discovery of adult neurogenesis, a major issue is the role of newborn neurons and the function-dependent regulation of adult neurogenesis. We decided to use an animal model with a relatively simple brain to address these questions. In the adult cricket brain as in mammals, new neurons are produced throughout...
journal_title:Chemical senses
pub_type: 杂志文章,评审
doi:10.1093/chemse/bjm010
更新日期:2007-05-01 00:00:00
abstract::Restriction of dietary sodium during gestation has major effects on taste function and anatomy in the offspring. The chorda tympani nerve of offspring that are maintained on sodium-reduced chow throughout life (NaDep) has reduced neurophysiological responses to sodium and altered morphology of its terminal field in th...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/25.6.719
更新日期:2000-12-01 00:00:00
abstract::Response accuracy and odor-sampling times were used to compare the ability of mice to detect (+)-carvone and (+)-terpinen-4-ol and to discriminate between enantiomers of carvone and of terpinen-4-ol. Except for increased odor sampling when mice were first exposed to the (+)-carvone odor, there was no difference in odo...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/bjm039
更新日期:2007-09-01 00:00:00
abstract::To analyze the mechanisms of perception and processing of pheromonal signals in vitro, we previously developed a new culture system for vomeronasal receptor neurons (VRNs), referred to as the vomeronasal pocket (VN pocket). However, very few VRNs were found to express the olfactory marker protein (OMP) and to have pro...
journal_title:Chemical senses
pub_type: 杂志文章
doi:10.1093/chemse/bji006
更新日期:2005-02-01 00:00:00