Abstract:
:Metastasis is a critical problem in the treatment of human lung cancer. Thus, a suitable animal model of metastasis of human lung cancer is required for in vivo biological and preclinical studies. In this study, we tried to establish a suitable model for this, using SCID mice. Neither human SCLC H69/VP cells (5 x 10(6)) nor squamous-cell carcinoma RERF-LC-AI cells (1 x 10(6)), injected through a tail vein, formed metastases in untreated SCID mice. Pre-treatment of SCID mice with anti-asialo GM1 serum resulted in only a few metastases of H69/VP cells, but pre-treatment with anti-mouse IL-2 receptor beta chain Ab (TM-beta 1) resulted in numerous lymph-node metastases 56 days after tumor inoculation. H69/VP-M cells, an in vivo-selected variant line, formed significant numbers of lymph-node metastases even in SCID mice pre-treated with anti-asialo GM1 serum. SCID mice depleted of NK cells by treatment with TM-beta 1 showed different patterns of metastasis when inoculated intravenously with the 2 different human lung cancer cell lines (H69/VP and RERF-LC-AI cells): H69/VP cells formed metastases mainly in systemic lymph nodes and the liver, whereas RERF-LC-AI cells formed metastases mainly in the liver and kidneys, with only a few in lymph nodes. A histopathological study showed that the metastatic colonies consisted of cancer cells. The numbers of metastatic colonies formed by the 2 cell lines increased with the number of cells inoculated. TM-beta 1 treatment of SCID mice efficiently removed NK cells from peripheral blood for at least 6 weeks, whereas, after treatment of the mice with anti-asialo GM1 serum, NK cells were recovered within 9 days. These findings suggest that NK-cell-depleted SCID mice may be useful as a model in biological and pre-clinical studies on metastasis of human lung cancer.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Yano S,Nishioka Y,Izumi K,Tsuruo T,Tanaka T,Miyasaka M,Sone Sdoi
10.1002/(SICI)1097-0215(19960717)67:2<211::AID-IJCsubject
Has Abstractpub_date
1996-07-17 00:00:00pages
211-7issue
2eissn
0020-7136issn
1097-0215pii
10.1002/(SICI)1097-0215(19960717)67:2<211::AID-IJCjournal_volume
67pub_type
杂志文章abstract::We have previously identified a region containing 16 CpGs within the MGMT CpG islands which is critical for the transcriptional control of MGMT (Malley, Acta Neuropathol 2011). To investigate the patterns and incidence of MGMT methylation in astrocytic and oligodendroglial tumors, we quantitatively assessed methylatio...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.26499
更新日期:2012-09-01 00:00:00
abstract::Sera from 450 individuals between the age of 1 and 80 years, representing the general population of the Czech Republic, were tested for the presence of antibodies to human-papillomavirus(HPV)-derived antigens. The following antigens were used: (i) HPV1 virions; (ii) HPV16, -18 and -33-virus-like particles (VLP); (iii)...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19980831)77:5<689::aid-ijc
更新日期:1998-08-31 00:00:00
abstract::The growth of a spontaneous adenocarcinoma (ADK-1t) of BALB/c mice (H-2d, MIsb) induces progressive hyporesponsiveness of spleen cells stimulated with PHA or irradiated leukocytes from DBA/2 strain (H-2d, MIsa) in mixed leukocyte culture. This hyporesponsiveness is due to the suppressor activity of macrophage-like cel...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910290616
更新日期:1982-06-15 00:00:00
abstract::The interactions of MCF-7 tumor cells with human vascular endothelial cells (EC) and subendothelial extracellular matrices (ECM) were morphologically observed by electron microscopy and quantitatively evaluated by labelling tumor cells with 111Indium-oxine. MCF-7 tumor cells adhered more rapidly to ECM than to the api...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910400416
更新日期:1987-10-15 00:00:00
abstract::A population-based case-control study of incident primary brain tumors in adults was carried out in Adelaide, Australia in the period 1987 through 1990. It included 110 subjects with newly diagnosed primary glioma, 60 subjects with meningioma and 417 controls selected from the Australian Electoral Roll and frequency-m...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910510105
更新日期:1992-04-22 00:00:00
abstract::An investigation was carried out to establish whether the reduction in catalase, glutathione peroxidase and superoxide dismutase activity, normally observed in liver tumours, is an early event and therefore of pathogenetic importance, or whether it is a late occurrence. Experiments performed on dimethylnitrosamine-tre...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910420529
更新日期:1988-11-15 00:00:00
abstract::Promoter hypermethylation of various tumor-related genes is extremely frequent in gastric carcinoma (GC) associated with Epstein-Barr virus (EBV). To investigate the significance of the promoter methylation in this type of GC, we examined the methylation densities of the promoter regions of p14ARF and p16INK4A in EBV-...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.20420
更新日期:2004-11-01 00:00:00
abstract::This large population-based study compared breast-conserving surgery with radiation therapy (BCT) with mastectomy on (long-term) breast cancer-specific (BCSS) and overall survival (OS), and investigated the influence of several prognostic factors. Patients with primary T1-2N0-2M0 breast cancer, diagnosed between 1999 ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.31034
更新日期:2018-01-01 00:00:00
abstract::The transcription factor OTX2 has been implicated as an oncogene in medulloblastoma, which is the most common malignant brain tumor in children. It is highly expressed in most medulloblastomas and amplified in a subset of them. To study the role OTX2 has in medulloblastoma we investigated the downstream pathway of OTX...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.26474
更新日期:2012-07-15 00:00:00
abstract::To investigate the association of childhood ALL and SES, we collected data on 507 children aged 0-14 years diagnosed with ALL between 1997 and 2002 from the population-based Cancer Registry of São Paulo, Brazil, covering 96 districts. Each child was assigned to an SES category based on the district of residence at dia...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.23738
更新日期:2008-10-15 00:00:00
abstract::The matrix metalloproteinase (MMP) family of proteins mediates various cellular pathways, including apoptosis and angiogenesis. Polymorphisms of MMP genes are associated with increased esophageal adenocarcinoma (EAC) risk. Gastroesophageal reflux disease (GERD) is an established EAC risk factor. We examined whether MM...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.27541
更新日期:2012-12-01 00:00:00
abstract::A Kunitz-type protease inhibitor, bikunin, downregulates expression of uPA and its receptor uPAR at the mRNA and protein levels in several types of tumor cells. Our recent work showed that, using a cDNA microarray analysis, pregnancy-associated plasma protein-A (PAPP-A) is a candidate bikunin target gene. To clarify h...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.11700
更新日期:2004-04-10 00:00:00
abstract::The cytogenetic response to G2-phase X-irradiation was examined in phytohemagglutinin-stimulated peripheral-blood lymphocytes from 69 individuals, a few of whom were cancer patients. The cancer patients had not received radiation or chemotherapy. The responses of cells arrested by Colcemid 30 to 90 min after X-irradia...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910540412
更新日期:1993-06-19 00:00:00
abstract::9-cis-Retinoic acid (9cRA) and 1alpha,25-dihydroxyvitamin D3 (1,25D) show promise as potential chemopreventive agents. We examined 9cRA and 1,25D, alone and in combination, for their potential to inhibit carcinogen (NNK)-induced lung carcinogenesis in A/J mice. A/J mice (n=14/group) were treated with 9cRA (7.5, 15, or...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.22462
更新日期:2007-04-01 00:00:00
abstract::The association between parity and hepatocellular carcinoma (HCC) was studied using a data-base generated by linking 2 Swedish nation-wide registries; the Cancer Registry and the Fertility Registry. Among women born between 1925 and 1960, 133 patients with HCC recorded in the Cancer Registry between 1958 and 1984 were...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910550508
更新日期:1993-11-11 00:00:00
abstract::The clinical pathological findings of eight cases of ovarian endodermal sinus tumour (yolk sac tumour) are presented. Histological exmination in all eight cases showed a typical endodermal sinus tumour pattern, and in six of the patients other tumour elements such as dysgerminoma, choriocarcinoma, malignant teratoma, ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910180505
更新日期:1976-11-15 00:00:00
abstract::Bone-marrow (BM) hematopoietic precursors are recruited into proliferative activity when colony-stimulating factors (CSF) are sequenced with chemotherapy (CT). Previous studies suggested that further CT can be safely administered only when the increased proliferative activity of these cells has subsided, because most ...
journal_title:International journal of cancer
pub_type: 临床试验,杂志文章
doi:10.1002/ijc.2910630508
更新日期:1995-11-27 00:00:00
abstract::A recent 2-dimensional gel electrophoresis study revealed several polypeptide differences between a strongly metastatic and a weakly metastatic clone from a single chemically induced murine fibrosarcoma (Jellum et al., 1984). To exclude the possibility that this was merely coincidental, the study is extended here to 2...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910410416
更新日期:1988-04-15 00:00:00
abstract::The occurrence of resistance to cytotoxic agents in tumor cells, associated with several phenotypic alterations, is one of the major obstacles to successful anticancer chemotherapy. A new strategy to overcome MDR of human cancer cells was studied, using BSAO, which generates cytotoxic products from spermine, H(2)O(2) ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.10310
更新日期:2002-05-01 00:00:00
abstract::Cytological screening for cervical cancer is hampered by high false negative rates. Inter-observer reproducibility needs optimizing. The potential of p16(INK4a) as a biomarker for cervical lesions was examined in a study of liquid-based cytology (LBC), HPV DNA testing by MY09/MY11 consensus PCR and type-specific PCRs ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.11589
更新日期:2004-03-01 00:00:00
abstract::Our study directly compares the performance of liquid-based (LBC) and conventional cytology for detecting high-grade cervical intraepithelial neoplasia and cancer (CIN 2+) in high-risk, previously unscreened women. As part of a larger randomized clinical trial assessing the efficacy and safety of a "screen and treat" ...
journal_title:International journal of cancer
pub_type: 杂志文章,随机对照试验
doi:10.1002/ijc.21434
更新日期:2006-02-15 00:00:00
abstract::Epidermal growth factor receptor (EGFR) plays an important role in essential cellular processes such as proliferation, survival and migration. Aberrant activation of EGFR is frequently found in human cancers of various origins and has been implicated in cancer pathogenesis. The therapeutic antibody cetuximab (Erbitux)...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.28585
更新日期:2014-06-01 00:00:00
abstract::Intratumoural (i.t.) injection of radio-iododeoxyuridine (IdUrd), a thymidine (dThd) analogue, is envisaged for targeted Auger electron- or beta-radiation therapy of glioblastoma. Here, biodistribution of [(125)I]IdUrd was evaluated 5 hr after i.t. injection in subcutaneous human glioblastoma xenografts LN229 after di...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.20112
更新日期:2004-05-20 00:00:00
abstract::Studies in Shanghai and in north-east China indicate that cigarette smoking is a major contributor to the high rates of lung cancer in those areas, but doubts persist regarding the influence of cigarette use on lung cancer rates in other areas of China. In addition, the risk of lung cancer associated with other method...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910510310
更新日期:1992-05-28 00:00:00
abstract::A series of 42 patients with malignant melanoma treated with BCG adjuvant immunotherapy were studied for sequential changes in cellular immune reactivity to non-specific mitogens. Lymphocyte preparations were made monthly and stored in a viable condition in liquid nitrogen. After 6 months of treatment, all lymphocyte ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910190104
更新日期:1977-01-01 00:00:00
abstract::Loss of heterozygosity (LOH) on chromosome 13 and the age of patients at operation were studied in 46 cases of retinoblastoma (RB) tumors, of which 25 were hereditary and 21 were non-hereditary. The frequency of LOH was 70% for all informative tumors, but significantly higher in non-hereditary tumors (90%) than in her...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910540609
更新日期:1993-07-30 00:00:00
abstract::Survival rates for osteosarcoma, the most common primary bone cancer, have changed little over the past three decades and are particularly low for patients with metastatic disease. We conducted a multi-institutional genome-wide association study (GWAS) to identify germline genetic variants associated with overall surv...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.31195
更新日期:2018-04-15 00:00:00
abstract::We describe an innovative strategy to quantify risk of cancer associated with varying levels of exposure to chronic parasitic infection through the identification of asymptomatic cases of cholangiocarcinoma within a population-based survey of Opisthorchis viverrini infection. Stool samples from 12,311 adults over age ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910590412
更新日期:1994-11-15 00:00:00
abstract::The age-standardized mortality rates for malignant melanoma of the skin in immigrants to New Zealand were compared with those of New Zealand-born non-Maoris. Immigrants from European countries had mortality rates that were generally similar to those prevailing in their countries of origin, although, among immigrants f...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910360208
更新日期:1985-08-15 00:00:00
abstract::An increased risk of prostate cancer associated with a family history of prostate cancer has been documented in multiple published reports. Risk has been shown to vary by degree of relationship and age of onset of disease in the affected relative. Several studies, using various designs, have estimated the relative ris...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.11466
更新日期:2003-12-10 00:00:00