Abstract:
:cDNA clones encoding S-antigen (arrestin) were isolated from the expression library constructed from porcine retina and sequenced. The 1490 base pair fragment contained a 1215 base pair open reading frame. From the nucleotide sequence, an amino acid sequence consisting of 405 residues was deduced and a molecular mass of 45,102 daltons was calculated. In order to determine whether the S-antigen mRNA transcript was expressed in anterior eye tissues, mRNA from ciliary non-pigmented epithelial cells and pigmented epithelial cells and iris was analysed by the reverse transcription polymerase chain reaction (PCR) using primers taken from sequences flanking the coding and non-coding regions of retinal S-antigen. Sequence analysis of the expected 611 base pairs in the 5' region and 672 base pairs in the 3' region of DNA fragments indicated that an identical mRNA for S-antigen was expressed in the anterior tissues examined. To investigate the in situ expression of S-antigen mRNA, 35S-labeled sense and antisense RNA probes were synthesized from the cDNA to label frozen sections of retina, ciliary body and iris and the treated sections were examined by autoradiography. The antisense probe labeled the layer between retinal pigmented epithelium and the outer nuclear layer of the retina, ciliary epithelium, and iris epithelium. From the results of sequencing PCR products and in situ hybridization, we concluded that, in porcine eye, the mRNA for S-antigen is expressed not only in the retina but also in the anterior tissues such as the ciliary epithelium and iris epithelium.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Singh AK,Kumar G,Shinohara T,Shichi Hdoi
10.1006/exer.1996.0036subject
Has Abstractpub_date
1996-03-01 00:00:00pages
299-308issue
3eissn
0014-4835issn
1096-0007pii
S0014-4835(96)90036-6journal_volume
62pub_type
杂志文章abstract::This study tests the hypothesis that choroidal myogenic autoregulation participates in the intraocular pressure (IOP) response to mean arterial pressure (MAP) by minimizing arterial-pressure-dependent changes in choroidal blood volume. To test this hypothesis, the IOP response to MAP was quantified before and after im...
journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:1994-05-01 00:00:00
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pub_type: 杂志文章,评审
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journal_title:Experimental eye research
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pub_type: 杂志文章,评审
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journal_title:Experimental eye research
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doi:10.1016/j.exer.2019.107755
更新日期:2019-10-01 00:00:00
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journal_title:Experimental eye research
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1997.0331
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更新日期:2019-11-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1994.1099
更新日期:1994-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1006/exer.1999.0796
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0194
更新日期:1997-02-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0177
更新日期:1997-01-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:2017-06-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2001.1110
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0167
更新日期:1996-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/0014-4835(90)90107-6
更新日期:1990-06-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2014-06-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2003.09.003
更新日期:2004-03-01 00:00:00
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更新日期:2009-06-15 00:00:00
abstract::Several scleral cross-linking (SXL) methods were suggested to increase the biomechanical stiffness of scleral tissue and therefore, to inhibit axial eye elongation in progressive myopia. In addition to scleral cross-linking and biomechanical effects caused by riboflavin and light irradiation such a treatment might ind...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2015.07.005
更新日期:2015-10-01 00:00:00