Protection in the late stages of paracetamol-induced liver cell injury with fructose, cyslosporin A and trifluoperazine.


:A fixed combination of the three components, fructose, cyclosporin A and trifluoperazine (FCAT), was found to protect in the late stage of paracetamol-induced liver cell injury both in vivo and in an in vivo/in vitro system. Rats pre-induced with phenobarbitone were given a paracetamol dose of 1 g/kg i.p. The combination of FCAT was given orally 3 h or 3 and 8 h after paracetamol and was able to afford protection as seen by measurements of plasma alanine transaminase (ALT) levels at 24 h. In the in vivo/in vitro system, rats pre-induced with phenobarbitone were dosed with paracetamol 1 g/kg i.p. to initiate injury and liver slices were then taken 3, 4 and 5 h later. The liver slices were then incubated for up to 18 h with the protective agents (FCAT) and the progression of injury followed. Injury was assessed by lactate dehydrogenase (LDH) leakage into the medium and potassium content of the slices. FCAT significantly reduced the injury even as assessed after 5 h in vivo initiation and 18 h progression in vitro. Mitochondrial membrane potential was also maintained in the FCAT-treated liver slices from paracetamol-treated rats as seen by the ability to maintain a gradient of triphenyl methyl phosphonium (TPMP+) between the cell and external medium. All three compounds are required for protection, indicating that more than one event is critical to the survival of the cell and each target point needs to be protected for effective long-term cell survival. The in vivo/in vitro system has been found to give a better comparability to the in vivo situation than injury models that take 6 h or less.






Beales D,McLean AE




Has Abstract


1996-03-18 00:00:00














  • Effect of vitamin E on selenium cytotoxicity in chick ganglia cultures.

    abstract::Short term exposure of cultured chick ganglia nerve fibers and neuroglia to selenite produced half maximal inhibitory growth effects in concentrations of 6.2 (2.9--13.0) X 10(-5) M and 1.1 (0.7--1.7) X 10(-4) M, respectively. Complete inhibition of growth for both cell types was apparent at 1 X 10(-3) M, while slight ...


    pub_type: 杂志文章


    authors: Obersteiner EJ,Sharma RP

    更新日期:1978-02-01 00:00:00

  • Inhibition of human erythrocyte Ca2+-ATPase by Zn2+.

    abstract::Recent investigations suggest that Ca2(+)-ATPase from fish gills is very sensitive to Zn2+ (Hogstrand et al., 1996. Am. J. Physiol. 270, R1141-R1147). The effect of free Zn2+ ion on the human erythrocyte plasma membrane Ca2(+)-ATPase was investigated to explore the possible extension of this finding to humans. Membran...


    pub_type: 杂志文章


    authors: Hogstrand C,Verbost PM,Wendelaar Bonga SE

    更新日期:1999-04-15 00:00:00

  • The developmental toxicity of uranium in mice.

    abstract::To evaluate the developmental toxicity of uranium, 5 groups of 20 pregnant Swiss mice were given by gavage daily doses of 0, 5, 10, 25 and 50 mg/kg of uranyl acetate dihydrate on gestational days 6-15. Cesarean sections were performed on all females on gestation day 18. Fetuses were examined for external, visceral, an...


    pub_type: 杂志文章


    authors: Domingo JL,Paternain JL,Llobet JM,Corbella J

    更新日期:1989-04-01 00:00:00

  • Bone metabolism and oxidative stress in postmenopausal rats with iron overload.

    abstract::Osteoporosis is associated with many etiological causes such as nutrition, cytokines, hormones, and aging. Recently, reactive oxygen species (ROS) are considered to be responsible for the aging process and osteoporosis. We investigated the relationship between ROS and bone metabolism in young female and postmenopausal...


    pub_type: 杂志文章


    authors: Isomura H,Fujie K,Shibata K,Inoue N,Iizuka T,Takebe G,Takahashi K,Nishihira J,Izumi H,Sakamoto W

    更新日期:2004-04-15 00:00:00

  • Human exposure assessment resources on the World Wide Web.

    abstract::Human exposure assessment is frequently noted as a weak link and bottleneck in the risk assessment process. Fortunately, the World Wide Web and Internet are providing access to numerous valuable sources of human exposure assessment-related information, along with opportunities for information exchange. Internet mailin...


    pub_type: 杂志文章


    authors: Schwela D,Hakkinen PJ

    更新日期:2004-05-20 00:00:00

  • Territrems B and C metabolism in human liver microsomes: major role of CYP3A4 and CYP3A5.

    abstract::Human liver microsomes, supersomes from baculovirus-transformed insect cells expressing different human CYP450 isoforms, and control and CYP3A4 cDNA-transfected V79 Chinese hamster cells were tested for their ability to metabolize territrem B (TRB) and territrem C (TRC). Two TRB metabolites, designated MB(2) and MB(4)...


    pub_type: 杂志文章


    authors: Peng FC,Chang CC,Yang CY,Edwards RJ,Doehmer J

    更新日期:2006-02-01 00:00:00

  • Melamine impairs spatial cognition and hippocampal synaptic plasticity by presynaptic inhibition of glutamatergic transmission in infant rats.

    abstract::The scandal of melamine-contamination has not been quite blown out, since the toxicity of melamine continues to raise concerns for public health. It has been well known that fetus and infant periods play the most important roles in brain development, whereas little has been done on the harmful effects of melamine on t...


    pub_type: 杂志文章


    authors: Yang J,An L,Yao Y,Yang Z,Zhang T

    更新日期:2011-11-18 00:00:00

  • The management of aircraft passenger survival in fire.

    abstract::The prime factors influencing survivability from 10 major fire-related public transport aircraft accidents were assessed. Regulatory requirements were assessed against derived criteria and alternate concepts evaluated to identify a preferred strategy for enhanced survival; the provision of passenger protective breathi...


    pub_type: 杂志文章,评审


    authors: Trimble EJ

    更新日期:1996-12-31 00:00:00

  • Immunotoxicity of a reconstituted polynuclear aromatic hydrocarbon mixture in B6C3F1 mice.

    abstract::Previous studies on the immunotoxicity of a complex mixture of polynuclear aromatic hydrocarbon (PAH) by-products from a manufactured gas plant indicated possible synergistic interactions which were investigated by determining the immunosuppressive effects of a reconstituted PAH mixture in female B6C3F1 mice challenge...


    pub_type: 杂志文章


    authors: Harper N,Steinberg M,Safe S

    更新日期:1996-05-03 00:00:00

  • Studies on the mechanism of paracetamol-induced protection against paracetamol hepatotoxicity.

    abstract::In rats, 3 days treatment with paracetamol (1 oral dose of 1 g/kg daily) produced a complete protection against the hepatotoxic actions of a further dose of paracetamol as documented by determination of serum enzyme activities (glutamic-oxaloacetic transaminase, (GOT), glutamic-pyruvic transaminase (GPT), sorbitol deh...


    pub_type: 杂志文章


    authors: Strubelt O,Siegers CP,Völpel M,Younes M

    更新日期:1979-02-01 00:00:00

  • The effects of the insecticide 2,2,2-trichloro-1-(3,4-dichlorophenyl)ethyl acetate on drug metabolism in the rat.

    abstract::The effects of 2,2,2-trichloro-1-(3,4-dichlorophenyl)ethyl acetate (Penfenate) on hepatic, renal and small intestinal drug-metabolizing enzyme activities, hepatic reduced glutathione content and urinary excretion of thioethers were studied in the rat. A single i.p. dose of Penfenate (500 mg/kg) decreased the body weig...


    pub_type: 杂志文章


    authors: Mäntylä E,Ahotupa M,Hietanen E,Vainio H

    更新日期:1983-07-01 00:00:00

  • Behavioral effects in adult mice exposed to perfluorooctane sulfonate (PFOS).

    abstract::Nowadays, very little information concerning the effects on behavior in mammals of perfluorooctane sulfonate (PFOS), a widely distributed persistent environmental pollutant, is available. In the present study, we assessed the behavioral effects of PFOS on 3 months old mice after 1 month of exposure to this pollutant. ...


    pub_type: 杂志文章


    authors: Fuentes S,Vicens P,Colomina MT,Domingo JL

    更新日期:2007-12-05 00:00:00

  • Kaempferol alleviates acute alcoholic liver injury in mice by regulating intestinal tight junction proteins and butyrate receptors and transporters.

    abstract::An impaired gut-liver axis is a potential factor that contributes to alcoholic liver disease. Specifically, ethanol decreases intestinal integrity and causes gut dysbiosis. Butyrate, a fermentation byproduct of gut microbiota, is negatively altered following acute ethanol exposure. This study aimed to determine whethe...


    pub_type: 杂志文章


    authors: Chen J,Xuan YH,Luo MX,Ni XG,Ling LQ,Hu SJ,Chen JQ,Xu JY,Jiang LY,Si WZ,Xu L,Gao H,Liu Z,Li H

    更新日期:2020-01-15 00:00:00

  • Waterborne gemfibrozil challenges the hepatic antioxidant defense system and down-regulates peroxisome proliferator-activated receptor beta (PPARbeta) mRNA levels in male goldfish (Carassius auratus).

    abstract::The lipid regulator gemfibrozil (GEM) is one of many human pharmaceuticals found in the aquatic environment. We previously demonstrated that GEM bioconcentrates in blood and reduces plasma testosterone levels in goldfish (Carassius auratus). In this study, we address the potential of an environmentally relevant waterb...


    pub_type: 杂志文章


    authors: Mimeault C,Trudeau VL,Moon TW

    更新日期:2006-12-07 00:00:00

  • Allopurinol suppresses 2-bromoethylamine and 1-methyl-4-phenylpyridinium ion (MPP(+))-induced hydroxyl radical generation in rat striatum.

    abstract::The present study was examined whether or not 2-bromoethyamine, a semicarbazide-sensitive amine oxidase (SSAO, EC; inhibitor, would increase an active dopaminergic neurotoxin, 1-methyl-4-phenylpyridinium ion (MPP(+))-induced hydroxyl radical ((*)OH) generation in the rat striatum. Rats were anesthetized, and ...


    pub_type: 杂志文章


    authors: Obata T

    更新日期:2006-01-20 00:00:00

  • Ultrafine titanium dioxide particles in the absence of photoactivation can induce oxidative damage to human bronchial epithelial cells.

    abstract::Ultrafine titanium dioxide (TiO(2)) particles have been shown to exhibit strong cytotoxicity when exposed to UVA radiation, but are regarded as a biocompatible material in the absence of photoactivation. In contrast to this concept, the present results indicate that anatase-sized (10 and 20 nm) TiO(2) particles in the...


    pub_type: 杂志文章


    authors: Gurr JR,Wang AS,Chen CH,Jan KY

    更新日期:2005-09-15 00:00:00

  • Repeated exposure to butenolide vapour: subacute study in Syrian golden hamsters.

    abstract::The subacute inhalation toxicity of butenolide was examined in hamsters by repeated exposure of 4 groups of 10 males and 10 females to butenolide vapour at concentrations of 0, 5.4, 25 and 130 ppm respectively (6 h/day, 5 days/week) for a period of 13 weeks. The effects found at 130 ppm included eye irritation, saliva...


    pub_type: 杂志文章


    authors: Feron VJ,Kruysse A,Immel HR,Til HP

    更新日期:1979-12-01 00:00:00

  • Trovafloxacin-induced replication stress sensitizes HepG2 cells to tumor necrosis factor-alpha-induced cytotoxicity mediated by extracellular signal-regulated kinase and ataxia telangiectasia and Rad3-related.

    abstract::Use of the fluoroquinolone antibiotic trovafloxacin (TVX) was restricted due to idiosyncratic, drug-induced liver injury (IDILI). Previous studies demonstrated that tumor necrosis factor-alpha (TNF) and TVX interact to cause death of hepatocytes in vitro that was associated with prolonged activation of c-Jun N-termina...


    pub_type: 杂志文章


    authors: Beggs KM,Maiuri AR,Fullerton AM,Poulsen KL,Breier AB,Ganey PE,Roth RA

    更新日期:2015-05-04 00:00:00

  • Receptor-dependent regulation of the CYP3A4 gene.

    abstract::A CYP3A4 promoter-reporter gene construct has been used to assess the ability of 16 known (in vivo) and putative (in vitro) inducers to transactivate a CYP3A4 reporter gene in HepG2 cells. With the exception of pravastatin, the remaining 15 compounds transactivated the CYP3A4 reporter gene with differing inductive abi...


    pub_type: 杂志文章


    authors: Gibson GG,el-Sankary W,Plant NJ

    更新日期:2002-12-27 00:00:00

  • Nickel toxicity in early embryogenesis in mice.

    abstract::The development of mouse embryos was studied after intraperitoneal injection of nickel chloride in the preimplantation period. A single intraperitoneal injection of NiCl2 . 6H2O in 0.154 M NaCl corresponding to 20 mg/kg body wt was given to groups of female mice on days 1, 2, 3, 4, 5 or 6 of gestation. Control groups ...


    pub_type: 杂志文章


    authors: Storeng R,Jonsen J

    更新日期:1981-01-01 00:00:00

  • Allergenicity evaluation of p-chloro-m-cresol and p-chloro-m-xylenol by non-radioactive murine local lymph-node assay and multiple-dose guinea pig maximization test.

    abstract::p-Chloro-m-cresol (PCMC) and p-chloro-m-xylenol (PCMX) are known to cause allergic contact dermatitis. For risk assessment of skin sensitizers, information on dose-response profiles in the induction and elicitation phases and cross-reactivity with analogous chemicals are important. In the non-radioactive local lymph-n...


    pub_type: 杂志文章


    authors: Yamano T,Shimizu M,Noda T

    更新日期:2003-08-28 00:00:00

  • Study of in vitro cytotoxicity of a water soluble organic arsenic compound, arsenosugar, in seaweed.

    abstract::In the present study, we demonstrated the cytotoxic effect of a dimethylarsenic compound in seaweed, (R)-(2',3'-dihydroxypropyl) 5- deoxy-5-dimethylarsinoyl-beta-D-riboside, namely arsenosugar (AsSug), on mammalian cells, murine macrophages, in comparison with that of an inorganic arsenical, arsenite, in vitro. More t...


    pub_type: 杂志文章


    authors: Sakurai T,Kaise T,Ochi T,Saitoh T,Matsubara C

    更新日期:1997-10-19 00:00:00

  • Chronic inhalation exposure to ozone and nitric acid elevates stress-inducible heat shock protein 70 in the rat lung.

    abstract::The ability of urban oxidant and acid air pollutants to induce heat shock proteins (HSPs) in the mammalian lung is not known. Such proteins are known to be correlated with environmental stress and pathophysiological conditions. In this study, stress-inducible HSP 70 was assessed by slot-blotting in rat lungs (N=10 per...


    pub_type: 杂志文章


    authors: Wong CG,Bonakdar M,Mautz WJ,Kleinman MT

    更新日期:1996-02-22 00:00:00

  • Influence of 2,3-dimercaptopropane-1-sulfonate and dimercaptosuccinic acid on the mobilization of mercury from tissues of rats pretreated with mercuric chloride, phenylmercury acetate or mercury vapors.

    abstract::The efficiency of the sodium salt of 2,3-dimercaptopropanesulfonic acid (DMPS) and meso-dimercaptosuccinic acid (DMSA) to mobilize mercury from tissues has been assessed in rats pretreated with different doses of HgCl2, phenylmercury acetate or exposed to different concentrations of mercury vapors. These pretreatments...


    pub_type: 杂志文章


    authors: Buchet JP,Lauwerys RR

    更新日期:1989-03-01 00:00:00

  • Evaluation of nitrofurantoin on the two stages of urinary bladder carcinogenesis in the rat.

    abstract::The possibility that nitrofurantoin is a complete carcinogen or is an initiator or promoter of urinary bladder carcinogenesis was evaluated in male weanling F344 rats. No increase in tumor incidence was observed in rats fed nitrofurantoin at a level of 0.187% of the diet for 2 years compared to a control group. Also, ...


    pub_type: 杂志文章


    authors: Hasegawa R,Murasaki G,St John MK,Zenser TV,Cohen SM

    更新日期:1990-06-01 00:00:00

  • [Toxicity by relay. III. Safety for the human consumer of the use of Carbadox, a feed additive for swine, as estimated by a 7 years relay toxicity on dogs].

    abstract::To check the possible toxicity risks of the meat from swine fed with a residue producing feed additive: Carbadox, a new methodology was used. It is the "Relay Toxicity". Swine were fed with a high dose of the additive and sacrificed without any withdrawal. In such conditions meat contains a high level of residues. The...


    pub_type: 杂志文章


    authors: Ferrando R,Truhaut R,Raynaud JP,Spanoghe JP

    更新日期:1978-10-01 00:00:00

  • Sodium benzoate attenuates D-serine induced nephrotoxicity in the rat.

    abstract::D-Serine causes selective necrosis to the straight portion of the rat renal proximal tubules. The onset is rapid, occurring within 3-4 h and accompanied by proteinuria, glucosuria and aminoaciduria. The metabolism of D-serine by D-amino acid oxidase (D-AAO) may be involved in the mechanism of toxicity. D-AAO is locali...


    pub_type: 杂志文章


    authors: Williams RE,Lock EA

    更新日期:2005-02-01 00:00:00

  • Inhibition of the activity of glutathione peroxidase by tertiary-butylhydroperoxide in cultured Chinese hamster cells and the role of cellular glutathione in the recovery of the activity.

    abstract::Treatment of Chinese hamster cells with 1 mM tertiary-butylhydroperoxide (t-BuOOH) for 1 h markedly inhibited the activity of glutathione peroxidase (GSH-Px). However, the activity returned to pre-inhibition levels within 1-2 h with post-treatment incubation. The inhibition of the activity of GSH-Px by t-BuOOH was enh...


    pub_type: 杂志文章


    authors: Ochi T

    更新日期:1992-01-01 00:00:00

  • Beneficial cardio-renovascular effects of a low-molecular-weight heparin-derivative on adriamycin-induced glycosaminoglycanuria and tissue lipid abnormalities.

    abstract::The present work includes a study on the glycosaminoglycanuric condition induced by adriamycin (ADR, a chemotherapeutic agent) and the accompanying secondary hyperlipidemia, wherein the treatment with a low-molecular-weight heparin-derivative (LMWH), certoparin, is evaluated for its protective role (if any) on these p...


    pub_type: 杂志文章


    authors: Deepa PR,Varalakshmi P

    更新日期:2005-07-01 00:00:00

  • Exploration of small RNA biomarkers for testicular injury in the serum exosomes of rats.

    abstract::Testicular injury is often observed in drug development. Serum hormones are usually used as noninvasive biomarkers for testicular injury; however, their sensitivities are low. Therefore, it is difficult to monitor testicular injury in drug development. In recent years, molecules in body fluid exosomes have attracted a...


    pub_type: 杂志文章


    authors: Kawata R,Kagawa T,Koya Y,Kajiyama H,Oda S,Yokoi T

    更新日期:2020-07-01 00:00:00