Abstract:
:Inorganic arsenic (iAs), a ubiquitous element and a natural drinking water contaminant, has been found to impair male reproductive function. However, the effect of long-term exposure to arsenic on testis damage and its underlying mechanisms still require further evaluation. In the study, male C57BL/6 mice (4 weeks) were treated with sodium arsenite at the doses of 5 or 50 ppm arsenic via drinking water for 180 days. Sperm count, histology in testes, oxidative stress biomarkers, cell cycle progress and apoptosis were assessed. Our results showed that arsenite seriously destroyed the structure of the testes and reduced the sperm count. Arsenite significantly decreased the activity of total superoxide dismutase (T-SOD) and glutathione (GSH) content but increased the levels of reactive oxygen species (ROS) and malondialdehyde (MDA) in testes. Furthermore, arsenite could induce G2/M phase arrest in testes, concurrent with a significant decrease in mRNA and protein levels of cdc2 and cyclin B1, the upregulation of p-cdc2, and an increase in mRNA levels of p53 and p21. Arsenite induced testicular apoptosis with a significant increase in Bax mRNA and protein levels, especially the caspase-3 activation. Testicular toxicity of the high dose group was stronger than that of the low dose group. In conclusion, testicular toxicity due to long-term exposure to arsenite may relate to oxidative damage, G2/M arrest and promoted apoptosis in the testes of mice, which contributed to the increased risk of spermatogenesis disorders and male infertility.
journal_name
Toxicologyjournal_title
Toxicologyauthors
Zeng Q,Yi H,Huang L,An Q,Wang Hdoi
10.1016/j.tox.2018.09.010subject
Has Abstractpub_date
2019-01-01 00:00:00pages
122-132eissn
0300-483Xissn
1879-3185pii
S0300-483X(18)30396-2journal_volume
411pub_type
杂志文章相关文献
TOXICOLOGY文献大全abstract::Recently, a large-scale field study in The Netherlands has focused on the effects of estrogenic contaminants on feral fish populations. The freshwater bream (Abramis brama) and the estuarine flounder (Platichthys flesus) were sampled at a large number of locations in the spring and autumn of 1999. Concentrations of th...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(02)00271-8
更新日期:2002-12-27 00:00:00
abstract::Static and rotary cultures of adult rabbit hepatocytes can be produced reproducibly following their isolation by means of a simplified, inexpensive recycling perfusion system. Light and electron microscopic examination of the cultures showed that these hepatocytes retained the morphological characteristics of fully di...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(82)90052-x
更新日期:1982-01-01 00:00:00
abstract::We have determined that the addition of 3-phenylamino-L-alanine (PAA), a recently reported contaminant in L-tryptophan implicated in the eosinophilia-myalgia syndrome, affects tryptophan binding by utilizing an in vitro measurement of 3H-tryptophan binding to hepatic nuclei or nuclear envelopes. PAA (10(-10) to 10(-4)...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(94)90058-2
更新日期:1994-01-26 00:00:00
abstract::Hexavalent chromium [Cr(VI)] compounds (e.g. chromates) are strong oxidants that readily enter cells where they are reduced to reactive Cr intermediates that can directly oxidize some cell components and can promote the generation of reactive oxygen and nitrogen species. Inhalation is a major route of exposure which d...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2011.01.001
更新日期:2011-03-15 00:00:00
abstract::Rats received 0, 200, 1000 and 5000 ppm metaldehyde in the diet for 2 years. Reproduction studies over three generations using the same dietary levels were carried out. In the third litter of each generation attention was paid to possible embryotoxic or teratogenic effects. The parameters studied included growth, food...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(75)90026-8
更新日期:1975-01-01 00:00:00
abstract::The objective of this study was to observe whether 1.8 GHz microwaves (MW) (SAR, 3 W/kg) exposure can influence human lymphocyte DNA damage induced by ultraviolet ray C (UVC). The lymphocytes, which were from three young healthy donors, were exposed to 254 nm UVC at the doses of 0.25, 0.5, 0.75, 1.0, 1.5 and 2.0 J m(-...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2007.01.019
更新日期:2007-04-11 00:00:00
abstract::In the current study, we used pathway-specific cDNA arrays to detect the transcriptional signature induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in vivo by studying simultaneously the expression profiles of 83 genes involved in apoptosis, cytokine production and angiogenesis. To this end, C57BL/6 mice were inj...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(02)00230-5
更新日期:2002-09-16 00:00:00
abstract::Food allergy is a significant health issue in the UK, affecting between 1 and 2% of adults and 5 and 8% of children. The UK Food Standards Agency seeks to ensure the safety of food allergic consumers by providing them with information and guidance on food choices. Since 1995, with the aim of addressing important polic...
journal_title:Toxicology
pub_type: 杂志文章,评审
doi:10.1016/j.tox.2010.08.007
更新日期:2010-12-30 00:00:00
abstract::It is reported that gambogic acid (GA), the main active compound of gamboge which is a dry resin extracted from Garcinia hanburyi tree, has potent antitumor activity both in vivo and in vitro. Activation of mitochondrial apoptotic pathway in cancer cells is one effective therapy for cancer treatment. In the present st...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2009.03.010
更新日期:2009-06-16 00:00:00
abstract::Simple and efficient ex vivo/in vitro screening systems for contact allergens are developed for alternative to conventional animal tests. We have previously proposed an ex vivo/in vitro proliferation assay as a first stage screening method with advantages over existing alternatives, using lymph node cells (LNC) from s...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(96)89846-5
更新日期:1996-11-15 00:00:00
abstract::The influence of the insertion of a liver into the perfusion circuit of a lung on the availability of benzo[a]pyrene and benzo[a]pyrene metabolites to the lung was examined. Perfused lungs from 5,6-benzoflavone pretreated rats release high quantities of free benzo[a]pyrene metabolites and conjugates into the perfusion...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(87)90100-4
更新日期:1987-08-01 00:00:00
abstract::Dioxins, including the most toxic congener, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), exert diverse biological effects in humans and animals. Host resistance, especially to virus infections, is considered one of the most sensitive targets of TCDD-toxicity, while a recent study showed that the vulnerability to TCDD o...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(01)00535-2
更新日期:2002-01-15 00:00:00
abstract::Carbon monoxide (CO) is a dangerous exogenous poison and an essential endogenous neurotransmitter. This gas when inhaled has an anaesthetic effect, which is poorly understood, but which may be fatal if compensatory mechanisms are exhausted, if cardiac oxygen (O(2)) needs exceed myocardial oxygenation and/or if apnoea ...
journal_title:Toxicology
pub_type: 杂志文章,评审
doi:10.1016/s0300-483x(03)00005-2
更新日期:2003-05-01 00:00:00
abstract::Salicylate has been found to be an inhibitor of fatty acid synthesis in isolated rat hepatocytes. Half-maximal inhibition of fatty acid synthesis occurs at approximately 2 mM. The inhibitory effect of salicylate on fatty acid synthesis is not relieve by the addition of acetate, suggesting that salicylate inhibits the ...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(82)90060-9
更新日期:1982-01-01 00:00:00
abstract::Several types of pesticides, such as organophosphates, phenoxyacetic acid, and carbamate have a high risk of affecting human health, causing allergic rhinitis and bronchial asthma-like diseases. We used our long-term sensitization method and a local lymph node assay to examine the allergic reactions caused by several ...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2009.05.014
更新日期:2009-07-10 00:00:00
abstract::Reactions of ethenyloxirane with amino (RNH2) and thiol (R'SH) nucleophiles occur by an SN2 mechanism involving competing ring cleavage at C-2 and C-3. In contrast, 2-ethenyl-2-methyloxirane reacts with amino (RNH2) and thiolate (R'S-) nucleophiles in methanol by regioselective SN2 attack at C-3 ("neo-pentyl" position...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(96)03458-0
更新日期:1996-10-28 00:00:00
abstract::Adrenocortical carcinoma (ACC) is an extremely rare and aggressive endocrine malignancy with a poor prognosis. The most common symptom of ACC is hypercortisolism (Cushing's syndrome), which has the highest mortality. Mitotane is used as a steroidogenesis inhibitor for Cushing's syndrome or as a chemical adrenalectomy ...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2012.04.007
更新日期:2012-08-16 00:00:00
abstract::The majority of human P450-dependent xenobiotic metabolism is carried out by polymorphic enzymes which can cause abolished, quantitatively or qualitatively altered or enhanced metabolism. The latter situation is due to stable duplication, multiduplication or amplification of active genes, most likely in response to di...
journal_title:Toxicology
pub_type: 杂志文章,评审
doi:10.1016/s0300-483x(02)00492-4
更新日期:2002-12-27 00:00:00
abstract::The free radical scavenging activities and inhibitory effect of lipid peroxidation of a delphinidin derivative in eggplant were investigated. Delphinidin-3-(p-coumaroylrutinoside)-5-glucoside (nasunin), an anthocyanin, was isolated as purple colored crystals from eggplant peels. Using electron spin resonance spectrome...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(00)00202-x
更新日期:2000-08-07 00:00:00
abstract::Oxybutynin hydrochloride, an anti-cholinergic/anti-spasmodic agent, was examined for its effect on fertility and peri-post natal development in the rat and its embryotoxic potential in the rat and rabbit. In the rat effects on reproductive performance included a slight increase in the incidence of foetal malformations...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(86)90044-2
更新日期:1986-07-01 00:00:00
abstract::Arsenic is an endocrine disruptor that promotes breast cancer (BCa) development. Estrogen synthesis, through aromatase activation, is essential for BCa promotion and progression through activating the G-coupled estrogen receptor 1 (GPER1), regulating rapid nongenomic effects involved in cell proliferation and migratio...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2020.152440
更新日期:2020-05-15 00:00:00
abstract::Mitochondria play a central role in the life and death of cells. These organelles serve as the major energy-producing power-house, whereby the generation of ATP is associated with the utilization of molecular oxygen. A significant fraction (2-3%) of molecular oxygen consumed by mitochondria may be reduced in a one-ele...
journal_title:Toxicology
pub_type: 杂志文章,评审
doi:10.1016/j.tox.2004.11.027
更新日期:2005-03-15 00:00:00
abstract::Although the production of polychlorinated biphenyl 77 (PCB77) has already been banned globally, PCB77 is still used for a wide range of commercial purposes. Previous evidence has demonstrated that the PCB77 administration should be responsible for the gut microbiota variations and the host health risk. However, the h...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2019.03.011
更新日期:2019-05-15 00:00:00
abstract::The subacute inhalation toxicity of butenolide was examined in hamsters by repeated exposure of 4 groups of 10 males and 10 females to butenolide vapour at concentrations of 0, 5.4, 25 and 130 ppm respectively (6 h/day, 5 days/week) for a period of 13 weeks. The effects found at 130 ppm included eye irritation, saliva...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(79)90020-9
更新日期:1979-12-01 00:00:00
abstract::The multiple cascades of signal transduction pathways that lead from receptors on the cell membrane to the nucleus, thus translating extracellular signals into changes in gene expression, may represent important targets for neurotoxic compounds. Among the biochemical steps and pathways that have been investigated are ...
journal_title:Toxicology
pub_type: 杂志文章,评审
doi:10.1016/s0300-483x(00)00435-2
更新日期:2001-03-07 00:00:00
abstract::The rodent Hershberger assay has been used predominantly by the pharmaceutical industry to evaluate androgenic and antiandrogenic chemicals for potential therapeutic use. However, this assay has not yet been formally validated and standardized for use in toxicology testing. There are many variations in the protocol us...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(01)00361-4
更新日期:2001-05-11 00:00:00
abstract::For the first time, kinetic parameters of the effect of tacrine, an anti-cholinesterase inhibitor of therapeutic potential in Alzheimer's disease has been studied on human retinal acetyl-cholinesterase (AChE). Tacrine inhibited the AChE activity in a concentration dependent manner, the IC(50) being about 45 nM. The Mi...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(00)00177-3
更新日期:2000-05-19 00:00:00
abstract::The effect of potassium cyanide on mitochondria DNA (mtDNA) in mouse brain was investigated in vivo and in vitro. When potassium cyanide (0, 0.1, 1.0 or 2.0 mM) was incubated with a crude mitochondria fraction prepared from mouse brain at 37 degrees C for 60 min, the damage of mtDNA was observed in a concentration-dep...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(02)00244-5
更新日期:2002-09-30 00:00:00
abstract::The contributions of the hepatic microsomal and cytosolic fractions in the metabolic activation of the promutagen 2-aminofluorene into mutagenic intermediates in the Ames test were investigated. Rat hepatic postmitochondrial, microsomal and cytosolic preparations could convert 2-aminofluorene to mutagens, the postmito...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(92)90050-o
更新日期:1992-01-01 00:00:00
abstract::Sixteen polycyclic aromatic hydrocarbons (PAHs) were screened for their ability to be directly cytotoxic to a cell line from the rainbow trout gill, RTgill-W1. Exposure times of 2 h or less were sufficient for direct cytotoxicity to be detected, which appeared to be caused by a common mechanism, the general perturbati...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(98)00030-4
更新日期:1998-05-15 00:00:00