Long-term arsenite exposure induces testicular toxicity by redox imbalance, G2/M cell arrest and apoptosis in mice.


:Inorganic arsenic (iAs), a ubiquitous element and a natural drinking water contaminant, has been found to impair male reproductive function. However, the effect of long-term exposure to arsenic on testis damage and its underlying mechanisms still require further evaluation. In the study, male C57BL/6 mice (4 weeks) were treated with sodium arsenite at the doses of 5 or 50 ppm arsenic via drinking water for 180 days. Sperm count, histology in testes, oxidative stress biomarkers, cell cycle progress and apoptosis were assessed. Our results showed that arsenite seriously destroyed the structure of the testes and reduced the sperm count. Arsenite significantly decreased the activity of total superoxide dismutase (T-SOD) and glutathione (GSH) content but increased the levels of reactive oxygen species (ROS) and malondialdehyde (MDA) in testes. Furthermore, arsenite could induce G2/M phase arrest in testes, concurrent with a significant decrease in mRNA and protein levels of cdc2 and cyclin B1, the upregulation of p-cdc2, and an increase in mRNA levels of p53 and p21. Arsenite induced testicular apoptosis with a significant increase in Bax mRNA and protein levels, especially the caspase-3 activation. Testicular toxicity of the high dose group was stronger than that of the low dose group. In conclusion, testicular toxicity due to long-term exposure to arsenite may relate to oxidative damage, G2/M arrest and promoted apoptosis in the testes of mice, which contributed to the increased risk of spermatogenesis disorders and male infertility.






Zeng Q,Yi H,Huang L,An Q,Wang H




Has Abstract


2019-01-01 00:00:00












  • Estrogenic effects in fish in The Netherlands: some preliminary results.

    abstract::Recently, a large-scale field study in The Netherlands has focused on the effects of estrogenic contaminants on feral fish populations. The freshwater bream (Abramis brama) and the estuarine flounder (Platichthys flesus) were sampled at a large number of locations in the spring and autumn of 1999. Concentrations of th...


    pub_type: 杂志文章


    authors: Vethaak AD,Lahr J,Kuiper RV,Grinwis GC,Rankouhi TR,Giesy JP,Gerritsen A

    更新日期:2002-12-27 00:00:00

  • Preparation and properties of primary maintenance cultures of adult rabbit hepatocytes.

    abstract::Static and rotary cultures of adult rabbit hepatocytes can be produced reproducibly following their isolation by means of a simplified, inexpensive recycling perfusion system. Light and electron microscopic examination of the cultures showed that these hepatocytes retained the morphological characteristics of fully di...


    pub_type: 杂志文章


    authors: Poole A,Chasseaud LF,Bridges JW

    更新日期:1982-01-01 00:00:00

  • Effect of 3-phenylamino-L-alanine on tryptophan binding to rat hepatic nuclear envelopes.

    abstract::We have determined that the addition of 3-phenylamino-L-alanine (PAA), a recently reported contaminant in L-tryptophan implicated in the eosinophilia-myalgia syndrome, affects tryptophan binding by utilizing an in vitro measurement of 3H-tryptophan binding to hepatic nuclei or nuclear envelopes. PAA (10(-10) to 10(-4)...


    pub_type: 杂志文章


    authors: Sidransky H,Verney E,Cosgrove JW,Latham PS

    更新日期:1994-01-26 00:00:00

  • The intracellular redox stress caused by hexavalent chromium is selective for proteins that have key roles in cell survival and thiol redox control.

    abstract::Hexavalent chromium [Cr(VI)] compounds (e.g. chromates) are strong oxidants that readily enter cells where they are reduced to reactive Cr intermediates that can directly oxidize some cell components and can promote the generation of reactive oxygen and nitrogen species. Inhalation is a major route of exposure which d...


    pub_type: 杂志文章


    authors: Myers JM,Antholine WE,Myers CR

    更新日期:2011-03-15 00:00:00

  • Long-term toxicity and reproduction studies with metaldehyde in rats.

    abstract::Rats received 0, 200, 1000 and 5000 ppm metaldehyde in the diet for 2 years. Reproduction studies over three generations using the same dietary levels were carried out. In the third litter of each generation attention was paid to possible embryotoxic or teratogenic effects. The parameters studied included growth, food...


    pub_type: 杂志文章


    authors: Verschuuren HG,Kroes R,Den Tonkelaar EM,Berkvens JM,Helleman PW,Van Esch GJ

    更新日期:1975-01-01 00:00:00

  • Evaluating the combinative effects on human lymphocyte DNA damage induced by ultraviolet ray C plus 1.8 GHz microwaves using comet assay in vitro.

    abstract::The objective of this study was to observe whether 1.8 GHz microwaves (MW) (SAR, 3 W/kg) exposure can influence human lymphocyte DNA damage induced by ultraviolet ray C (UVC). The lymphocytes, which were from three young healthy donors, were exposed to 254 nm UVC at the doses of 0.25, 0.5, 0.75, 1.0, 1.5 and 2.0 J m(-...


    pub_type: 杂志文章


    authors: Baohong W,Lifen J,Lanjuan L,Jianlin L,Deqiang L,Wei Z,Jiliang H

    更新日期:2007-04-11 00:00:00

  • Analysis of 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced gene expression profile in vivo using pathway-specific cDNA arrays.

    abstract::In the current study, we used pathway-specific cDNA arrays to detect the transcriptional signature induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in vivo by studying simultaneously the expression profiles of 83 genes involved in apoptosis, cytokine production and angiogenesis. To this end, C57BL/6 mice were inj...


    pub_type: 杂志文章


    authors: Zeytun A,McKallip RJ,Fisher M,Camacho I,Nagarkatti M,Nagarkatti PS

    更新日期:2002-09-16 00:00:00

  • Food allergy--science and policy needs--The UK Food Standards Agency Research Programme.

    abstract::Food allergy is a significant health issue in the UK, affecting between 1 and 2% of adults and 5 and 8% of children. The UK Food Standards Agency seeks to ensure the safety of food allergic consumers by providing them with information and guidance on food choices. Since 1995, with the aim of addressing important polic...


    pub_type: 杂志文章,评审


    authors: Buck J,Hattersley S,Kimber I

    更新日期:2010-12-30 00:00:00

  • Reactive oxygen species accumulation contributes to gambogic acid-induced apoptosis in human hepatoma SMMC-7721 cells.

    abstract::It is reported that gambogic acid (GA), the main active compound of gamboge which is a dry resin extracted from Garcinia hanburyi tree, has potent antitumor activity both in vivo and in vitro. Activation of mitochondrial apoptotic pathway in cancer cells is one effective therapy for cancer treatment. In the present st...


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    authors: Nie F,Zhang X,Qi Q,Yang L,Yang Y,Liu W,Lu N,Wu Z,You Q,Guo Q

    更新日期:2009-06-16 00:00:00

  • Improved ex vivo/in vitro lymph node cell proliferation assay in guinea pigs for a screening test of contact hypersensitivity of chemical compounds.

    abstract::Simple and efficient ex vivo/in vitro screening systems for contact allergens are developed for alternative to conventional animal tests. We have previously proposed an ex vivo/in vitro proliferation assay as a first stage screening method with advantages over existing alternatives, using lymph node cells (LNC) from s...


    pub_type: 杂志文章


    authors: Kashima R,Oyake Y,Okada J,Ikeda Y

    更新日期:1996-11-15 00:00:00

  • Metabolism of benzo[a]pyrene in the combined rat liver--lung perfusion system.

    abstract::The influence of the insertion of a liver into the perfusion circuit of a lung on the availability of benzo[a]pyrene and benzo[a]pyrene metabolites to the lung was examined. Perfused lungs from 5,6-benzoflavone pretreated rats release high quantities of free benzo[a]pyrene metabolites and conjugates into the perfusion...


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    更新日期:1987-08-01 00:00:00

  • Effect of low-dose 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on influenza A virus-induced mortality in mice.

    abstract::Dioxins, including the most toxic congener, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), exert diverse biological effects in humans and animals. Host resistance, especially to virus infections, is considered one of the most sensitive targets of TCDD-toxicity, while a recent study showed that the vulnerability to TCDD o...


    pub_type: 杂志文章


    authors: Nohara K,Izumi H,Tamura Si,Nagata R,Tohyama C

    更新日期:2002-01-15 00:00:00

  • The clinical toxicology of carbon monoxide.

    abstract::Carbon monoxide (CO) is a dangerous exogenous poison and an essential endogenous neurotransmitter. This gas when inhaled has an anaesthetic effect, which is poorly understood, but which may be fatal if compensatory mechanisms are exhausted, if cardiac oxygen (O(2)) needs exceed myocardial oxygenation and/or if apnoea ...


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  • Inhibition of hepatic lipogenesis by salicylate.

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    pub_type: 杂志文章


    authors: Beynen AC,Buechler KF,van der Molen AJ,Geelen MJ

    更新日期:1982-01-01 00:00:00

  • Allergic reaction induced by dermal and/or respiratory exposure to low-dose phenoxyacetic acid, organophosphorus, and carbamate pesticides.

    abstract::Several types of pesticides, such as organophosphates, phenoxyacetic acid, and carbamate have a high risk of affecting human health, causing allergic rhinitis and bronchial asthma-like diseases. We used our long-term sensitization method and a local lymph node assay to examine the allergic reactions caused by several ...


    pub_type: 杂志文章


    authors: Fukuyama T,Tajima Y,Ueda H,Hayashi K,Shutoh Y,Harada T,Kosaka T

    更新日期:2009-07-10 00:00:00

  • Studies on the molecular toxicology of buta-1,3-diene and isoprene epoxides.

    abstract::Reactions of ethenyloxirane with amino (RNH2) and thiol (R'SH) nucleophiles occur by an SN2 mechanism involving competing ring cleavage at C-2 and C-3. In contrast, 2-ethenyl-2-methyloxirane reacts with amino (RNH2) and thiolate (R'S-) nucleophiles in methanol by regioselective SN2 attack at C-3 ("neo-pentyl" position...


    pub_type: 杂志文章


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    更新日期:1996-10-28 00:00:00

  • Mitotane exhibits dual effects on steroidogenic enzymes gene transcription under basal and cAMP-stimulating microenvironments in NCI-H295 cells.

    abstract::Adrenocortical carcinoma (ACC) is an extremely rare and aggressive endocrine malignancy with a poor prognosis. The most common symptom of ACC is hypercortisolism (Cushing's syndrome), which has the highest mortality. Mitotane is used as a steroidogenesis inhibitor for Cushing's syndrome or as a chemical adrenalectomy ...


    pub_type: 杂志文章


    authors: Lin CW,Chang YH,Pu HF

    更新日期:2012-08-16 00:00:00

  • Polymorphism of cytochrome P450 and xenobiotic toxicity.

    abstract::The majority of human P450-dependent xenobiotic metabolism is carried out by polymorphic enzymes which can cause abolished, quantitatively or qualitatively altered or enhanced metabolism. The latter situation is due to stable duplication, multiduplication or amplification of active genes, most likely in response to di...


    pub_type: 杂志文章,评审


    authors: Ingelman-Sundberg M

    更新日期:2002-12-27 00:00:00

  • Antioxidant activity of nasunin, an anthocyanin in eggplant peels.

    abstract::The free radical scavenging activities and inhibitory effect of lipid peroxidation of a delphinidin derivative in eggplant were investigated. Delphinidin-3-(p-coumaroylrutinoside)-5-glucoside (nasunin), an anthocyanin, was isolated as purple colored crystals from eggplant peels. Using electron spin resonance spectrome...


    pub_type: 杂志文章


    authors: Noda Y,Kneyuki T,Igarashi K,Mori A,Packer L

    更新日期:2000-08-07 00:00:00

  • Reproductive toxicity studies with oxybutynin hydrochloride.

    abstract::Oxybutynin hydrochloride, an anti-cholinergic/anti-spasmodic agent, was examined for its effect on fertility and peri-post natal development in the rat and its embryotoxic potential in the rat and rabbit. In the rat effects on reproductive performance included a slight increase in the incidence of foetal malformations...


    pub_type: 杂志文章


    authors: Edwards JA,Reid YJ,Cozens DD

    更新日期:1986-07-01 00:00:00

  • Role of aromatase activation on sodium arsenite-induced proliferation, migration, and invasion of MDA-MB-231 and MDA-MB-453 breast cancer cell lines.

    abstract::Arsenic is an endocrine disruptor that promotes breast cancer (BCa) development. Estrogen synthesis, through aromatase activation, is essential for BCa promotion and progression through activating the G-coupled estrogen receptor 1 (GPER1), regulating rapid nongenomic effects involved in cell proliferation and migratio...


    pub_type: 杂志文章


    authors: Reyes-Vázquez L,Hernández AJA,Calderón-Aranda ES

    更新日期:2020-05-15 00:00:00

  • An epigrammatic (abridged) recounting of the myriad tales of astonishing deeds and dire consequences pertaining to nitric oxide and reactive oxygen species in mitochondria with an ancillary missive concerning the origins of apoptosis.

    abstract::Mitochondria play a central role in the life and death of cells. These organelles serve as the major energy-producing power-house, whereby the generation of ATP is associated with the utilization of molecular oxygen. A significant fraction (2-3%) of molecular oxygen consumed by mitochondria may be reduced in a one-ele...


    pub_type: 杂志文章,评审


    authors: Heck DE,Kagan VE,Shvedova AA,Laskin JD

    更新日期:2005-03-15 00:00:00

  • Gut microbiota characterization and lipid metabolism disorder found in PCB77-treated female mice.

    abstract::Although the production of polychlorinated biphenyl 77 (PCB77) has already been banned globally, PCB77 is still used for a wide range of commercial purposes. Previous evidence has demonstrated that the PCB77 administration should be responsible for the gut microbiota variations and the host health risk. However, the h...


    pub_type: 杂志文章


    authors: Chi Y,Wang H,Lin Y,Lu Y,Huang Q,Ye G,Dong S

    更新日期:2019-05-15 00:00:00

  • Repeated exposure to butenolide vapour: subacute study in Syrian golden hamsters.

    abstract::The subacute inhalation toxicity of butenolide was examined in hamsters by repeated exposure of 4 groups of 10 males and 10 females to butenolide vapour at concentrations of 0, 5.4, 25 and 130 ppm respectively (6 h/day, 5 days/week) for a period of 13 weeks. The effects found at 130 ppm included eye irritation, saliva...


    pub_type: 杂志文章


    authors: Feron VJ,Kruysse A,Immel HR,Til HP

    更新日期:1979-12-01 00:00:00

  • Intracellular signal transduction pathways as targets for neurotoxicants.

    abstract::The multiple cascades of signal transduction pathways that lead from receptors on the cell membrane to the nucleus, thus translating extracellular signals into changes in gene expression, may represent important targets for neurotoxic compounds. Among the biochemical steps and pathways that have been investigated are ...


    pub_type: 杂志文章,评审


    authors: Costa LG,Guizzetti M,Lu H,Bordi F,Vitalone A,Tita B,Palmery M,Valeri P,Silvestrini B

    更新日期:2001-03-07 00:00:00

  • Dissection and weighing of accessory sex glands after formalin fixation, and a 5-day assay using young mature rats are reliable and feasible in the Hershberger assay.

    abstract::The rodent Hershberger assay has been used predominantly by the pharmaceutical industry to evaluate androgenic and antiandrogenic chemicals for potential therapeutic use. However, this assay has not yet been formally validated and standardized for use in toxicology testing. There are many variations in the protocol us...


    pub_type: 杂志文章


    authors: Yamada T,Sunami O,Kunimatsu T,Kamita Y,Okuno Y,Seki T,Nakatsuka I,Matsuo M

    更新日期:2001-05-11 00:00:00

  • Kinetic analysis of the toxicological effect of tacrine (Cognex) on human retinal acetylcholinesterase activity.

    abstract::For the first time, kinetic parameters of the effect of tacrine, an anti-cholinesterase inhibitor of therapeutic potential in Alzheimer's disease has been studied on human retinal acetyl-cholinesterase (AChE). Tacrine inhibited the AChE activity in a concentration dependent manner, the IC(50) being about 45 nM. The Mi...


    pub_type: 杂志文章


    authors: Alhomida AS,Al-Rajhi AA,Kamal MA,Al-Jafari AA

    更新日期:2000-05-19 00:00:00

  • Melatonin attenuates brain mitochondria DNA damage induced by potassium cyanide in vivo and in vitro.

    abstract::The effect of potassium cyanide on mitochondria DNA (mtDNA) in mouse brain was investigated in vivo and in vitro. When potassium cyanide (0, 0.1, 1.0 or 2.0 mM) was incubated with a crude mitochondria fraction prepared from mouse brain at 37 degrees C for 60 min, the damage of mtDNA was observed in a concentration-dep...


    pub_type: 杂志文章


    authors: Yamamoto HA,Mohanan PV

    更新日期:2002-09-30 00:00:00

  • A cytosolic oxygenase activity involved in the bioactivation of 2-aminofluorene.

    abstract::The contributions of the hepatic microsomal and cytosolic fractions in the metabolic activation of the promutagen 2-aminofluorene into mutagenic intermediates in the Ames test were investigated. Rat hepatic postmitochondrial, microsomal and cytosolic preparations could convert 2-aminofluorene to mutagens, the postmito...


    pub_type: 杂志文章


    authors: Leist M,Ayrton AD,Ioannides C

    更新日期:1992-01-01 00:00:00

  • Ability of 16 priority PAHs to be directly cytotoxic to a cell line from the rainbow trout gill.

    abstract::Sixteen polycyclic aromatic hydrocarbons (PAHs) were screened for their ability to be directly cytotoxic to a cell line from the rainbow trout gill, RTgill-W1. Exposure times of 2 h or less were sufficient for direct cytotoxicity to be detected, which appeared to be caused by a common mechanism, the general perturbati...


    pub_type: 杂志文章


    authors: Schirmer K,Dixon DG,Greenberg BM,Bols NC

    更新日期:1998-05-15 00:00:00