A method for determining the position and size of optimal sequence regions for phylogenetic analysis.

Abstract:

:The availability of fast and accurate sequencing procedures along with the use of PCR has led to a proliferation of studies of variability at the molecular level in populations. Nevertheless, it is often impractical to examine long genomic stretches and a large number of individuals at the same time. In order to optimize this kind of study, we suggest a heuristic procedure for detection of the shortest region whose informational content can be considered sufficient for significant phylogenetic reconstruction. The method is based on the comparison of the pairwise genetic distances obtained from a set of sequences of reference to those obtained for different windows of variable size and position by means of a simple index. We also present an approach for testing whether the informative content in the stretches selected in this way is significantly different from the corresponding content shown by the larger genomic regions used as reference. Application of this test to the analysis of the VP1 protein gene of foot-and-mouth-disease type C virus allowed us to define optimal stretches whose informative content is not significantly different from that displayed by the complete VP1 sequence. We showed that the predictions made for type C sequences are valid for type O sequences, indicating that the results of the procedure are consistent.

journal_name

J Mol Evol

authors

Martin MJ,González-Candelas F,Sobrino F,Dopazo J

doi

10.1007/BF00173194

subject

Has Abstract

pub_date

1995-12-01 00:00:00

pages

1128-38

issue

6

eissn

0022-2844

issn

1432-1432

journal_volume

41

pub_type

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