First pregnancies and livebirths from transfer of sodium alginate encapsulated embryos in a rodent model.

Abstract:

OBJECTIVE:To determine the effect of sodium alginate encapsulation of rodent embryos on in vitro embryonic cleavage rates, implantation rates, and livebirth rates, and to find the in vivo degradation time for the capsules. DESIGN:Studies were conducted using both CB6F1 mice and Golden Syrian hamsters. RESULTS:Capsules made with 3.0% sodium alginate degraded in vivo within 24 to 48 hours after transfer. In vitro embryonic cleavage of encapsulated embryos was not impaired, nor were implantation rates in CB6F1 mice. Finally, 8.6% of transferred encapsulated embryos resulted in livebirths. CONCLUSIONS:Encapsulation of rodent embryos in 3.0% sodium alginate is not detrimental to embryonic development, implantation rates, or fetal development. Because the capsule degrades within 48 hours after transfer, encapsulating embryos may be beneficial for human in vitro fertilization and embryo transfer.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Adaniya GK,Rawlins RG,Quigg JM,Roblero L,Miller IF,Zaneveld LJ

doi

10.1016/s0015-0282(16)55814-2

subject

Has Abstract

pub_date

1993-03-01 00:00:00

pages

652-6

issue

3

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(16)55814-2

journal_volume

59

pub_type

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