Abstract:
OBJECTIVE:To evaluate the effects of cryopreservation on the survival, cleavage, and morphology of embryos and on the implantation and embryonic loss rates of human zygotes obtained after ICSI compared with frozen-thawed zygotes obtained after traditional IVF. A further objective was to evaluate the same parameters in nonfrozen sibling ICSI and IVF zygotes and to compare them with corresponding frozen-thawed zygotes. DESIGN:Open, retrospective, comparative study. SETTING:University-associated assisted reproductive program. PATIENT(S):Couples with severe male factor infertility and couples undergoing IVF during the same period. INTERVENTION(S):A cohort of 408 ICSI zygotes and 299 IVF zygotes was frozen in 1,2 propanediol and sucrose using a slow-freezing protocol. Both groups of zygotes were frozen at approximately the same time after microassisted or conventional insemination. One hundred and eighty-seven ICSI and 110 IVF frozen zygotes were rapidly thawed during 44 ICSI cycles and 24 IVF cycles. Zygotes that appeared to have survived were cultured for 24 hours, and most of these embryos that were morphologically normal were transferred into patients. MAIN OUTCOME MEASURE(S):Survival rate (morphologically intact after thawing), cleavage rate and morphology of embryos, implantation rate, and the incidence of embryonic losses. RESULT(S):Except for survival rates, for which both ICSI and IVF frozen-thawed zygotes showed similar and relatively high values (87.7% and 89.1%), the outcomes of other parameters evaluated were significantly different. Thus, from a total of 128 ICSI and 68 IVF embryos transferred, 14 (10.9%) and 17 (25.0%) implanted in 44 ICSI and 24 IVF frozen-thawed cycles, respectively. This difference in implantation corresponded with the rate of cleavage and morphology of the replaced embryos; the embryos that developed from frozen-thawed IVF zygotes cleaved faster and were more regular compared to the frozen-thawed ICSI zygotes. The embryonic loss rate was 57.1% for cryopreserved ICSI zygotes and 11.8% for IVF zygotes. On the other hand, no difference in cleavage pattern, embryo morphology, implantation, and embryonic loss rates was found between nonfrozen sibling ICSI and IVF zygotes. CONCLUSION(S):The zygotes arising from ICSI cycles survived cryopreservation at a rate similar to IVF zygotes, but their ability to implant and develop further was probably affected by the cryopreservation procedure. The timing of zygote freezing was considered to be the principal reason for the lower developmental potential of frozen-thawed ICSI zygotes in the present study.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Macas E,Imthurn B,Borsos M,Rosselli M,Maurer-Major E,Keller PJdoi
10.1016/s0015-0282(98)00021-1subject
Has Abstractpub_date
1998-04-01 00:00:00pages
630-5issue
4eissn
0015-0282issn
1556-5653pii
S0015-0282(98)00021-1journal_volume
69pub_type
杂志文章abstract::The effects of intravaginal application of nonoxynol-9 (N-9) on hematological parameters, routine liver function biochemistry (bilirubin, serum bile acid, SGOT, SGPT, GGT, alkaline phosphatase), and serum lipids (cholesterol and triglyceride) were evaluated in 10 normal women who received 150 mg N-9 daily for 14 conse...
journal_title:Fertility and sterility
pub_type: 信件
doi:10.1016/s0015-0282(16)46058-9
更新日期:1982-02-01 00:00:00
abstract::The International Committee Monitoring Assisted Reproductive Technologies (ICMART) is an independent international nonprofit organization that has taken a leading role in the development, collection, and dissemination of worldwide data on assisted reproductive technology (ART). Information on availability, efficacy, a...
journal_title:Fertility and sterility
pub_type:
doi:10.1016/j.fertnstert.2006.04.018
更新日期:2006-07-01 00:00:00
abstract:OBJECTIVE:To assess the molecular signatures underlying endometrial disorder using cDNA microarray. DESIGN:Gene expression-based oligonucleotide array of the normal endometrium. SETTING:University hospital. PATIENT(S):Humans. INTERVENTION(S):Endometrial tissues were obtained from 28 normal cycling women undergoing ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2009.01.130
更新日期:2010-06-01 00:00:00
abstract::Whatever the mechanism of such improvements may be, the results in this study point out that coitus interruptus in the human may not be the method of choice for collection of semen specimens, especially in patients with spermatogenic dysfunctions such as hypospermia, oligospermia, and asthenospermia. It should also be...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)56779-x
更新日期:1994-06-01 00:00:00
abstract::A 30-year-old nulliparous woman, who was infertile for greater than 3 years, conceived after the procedures of a simple method of zona cutting, cryopreservation, and ZIFT. This case illustrates: (1) acid medium, chymotrypsin, or sucrose are not needed for the procedure of zona cutting; (2) the zygotes resulting from z...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)54139-9
更新日期:1991-02-01 00:00:00
abstract:OBJECTIVE:To introduce an innovation that combines single port laparoscopic and Wharton-Sheares-George cervicovaginal reconstruction using a small intestinal submucosa (SIS) graft in a patient with congenital cervical atresia, absence of vagina, and incomplete uterine septum (U2aC4V4). DESIGN:Video article introducing...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2019.11.011
更新日期:2020-03-01 00:00:00
abstract:OBJECTIVE:To define the mechanism(s) underlying an association between asthenozoospermia and elevated blood, seminal plasma, and testicular cadmium levels in infertile human males using a rat model of environmentally relevant cadmium exposures. SETTING:University medical center andrology research laboratory. ANIMAL(S...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2007.12.035
更新日期:2008-02-01 00:00:00
abstract:OBJECTIVE:To estimate the prevalence and the characteristics of intrauterine adhesions after cold loop resectoscopic myomectomy. DESIGN:Retrospective study. SETTING:Endoscopic gynecologic center. PATIENT(S):688 women with one or more G1-G2 myomas. INTERVENTION(S):Cold loop resectoscopic myomectomy and diagnostic hy...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2013.09.032
更新日期:2014-01-01 00:00:00
abstract::Women bear the predominant burden of our obesogenic environment, with a higher incidence of obesity than men, more impact on their fertility and success with treatment, and significant maternal and perinatal morbidity and mortality. In this series, the causes, consequences, and solutions regarding the obesity pandemic...
journal_title:Fertility and sterility
pub_type:
doi:10.1016/j.fertnstert.2017.02.110
更新日期:2017-04-01 00:00:00
abstract:OBJECTIVE:To study the interobserver reproducibility of our new ultrasonographic mapping system to define the type and extension of uterine adenomyosis. DESIGN:Interobserver study involving two observers with different medical backgrounds and gynecological ultrasound experience. SETTING:University hospital. PATIENTS...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2018.06.031
更新日期:2018-11-01 00:00:00
abstract:OBJECTIVE:To study nucleation status in two- and four-cell embryos and its effect on reproductive outcomes. DESIGN:Retrospective cohort study. SETTING:University-affiliated private center. PATIENT(S):A total of 1,679 embryos from 940 oocyte donation cycles from May 2012 to May 2014. INTERVENTION(S):None. MAIN OUTC...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2016.03.036
更新日期:2016-08-01 00:00:00
abstract:OBJECTIVE:To elucidate the role of vitamin D in reproduction by examining the relationship between recipient vitamin D levels and pregnancy rates in donor-recipient IVF cycles. DESIGN:Retrospective cohort study. SETTING:Academic tertiary care center. PATIENT(S):Ninety-nine recipients of egg donation at University of...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2013.10.008
更新日期:2014-02-01 00:00:00
abstract:OBJECTIVE:To assess the effect of gonadotropin-releasing hormone agonist (GnRH-a) on pituitary suppression, subsequent ovarian response, and results of in vitro fertilization (IVF) treatments in polycystic ovarian syndrome (PCOS) patients. DESIGN:Randomized prospective study. SETTING:In vitro fertilization program an...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)54847-x
更新日期:1992-02-01 00:00:00
abstract::Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), which is known to exert death-inducing as well as nonapoptotic functions, has been shown to be expressed by the early trophoblast. Here we report that TRAIL has no apoptotic effects on human endometrial stromal cells, but differentially regulates cytokin...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2009.04.017
更新日期:2009-10-01 00:00:00
abstract:OBJECTIVE:To compare the effects of gonadotropin-releasing hormone agonist (GnRH-a) initiation either preceding or concurrent with controlled ovarian hyperstimulation (COH) in patients undergoing in vitro fertilization-embryo transfer (IVF-ET). DESIGN:Fifty-five patients were prospectively randomized to receive either...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0015-0282(16)55322-9
更新日期:1992-10-01 00:00:00
abstract:OBJECTIVES:To test the hypothesis that fertility is reduced in baboons with endometriosis. DESIGN:Prospective controlled follow-up study. SETTING:Institute of Primate Research, Nairobi Kenya. SUBJECTS:Seventy-one baboons, including 34 controls (normal pelvis) and 37 animals with histologically proven endometriosis (...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1996-11-01 00:00:00
abstract:OBJECTIVE:To assess cycle outcomes when antimüllerian hormone (AMH) is ultralow (≤0.16 ng/mL) and to determine which parameters contribute to the probability of cycle cancellation and/or outcome. DESIGN:Retrospective analysis. SETTING:Not applicable. PATIENT(S):5,087 (7.3%) fresh and 243 (1.5%) thawed cycles with ul...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2015.10.004
更新日期:2016-02-01 00:00:00
abstract:OBJECTIVE:To study whether specific congenital anomalies occur more often with a history of subfertility and/or the use of in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI). DESIGN:Case-only analyses. SETTING:Not applicable. PATIENT(S):We included live births, stillbirths, and terminated pregnanci...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2014.12.113
更新日期:2015-04-01 00:00:00
abstract::The presence of ovarian cysts may compromise the success of in vitro fertilization (IVF) and gamete intrafallopian transfer (GIFT). We prospectively studied 212 consecutive ovulation induction cycles in 120 patients for IVF and/or GIFT. A baseline cyst was defined as any intraovarian cystic structure greater than or e...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)54123-5
更新日期:1991-02-01 00:00:00
abstract:OBJECTIVE:To assess the association between recent cigarette smoking (CS) in female and male partners and assisted reproduction technology (ART) outcomes. DESIGN:Cohort prospective study. SETTING:University ART program in Chile. PATIENT(S):One hundred sixty-six couples seeking pregnancy through ART. INTERVENTION(S)...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2008.09.073
更新日期:2010-01-01 00:00:00
abstract:OBJECTIVE:To assess the minimal effective dose of a GnRH antagonist (Cetrorelix; Asta Medical; Frankfurt, Germany) to prevent premature LH surge in patients undergoing controlled ovarian hyperstimulation (COH) for assisted reproductive technologies. DESIGN:In 69 patients COH was carried out with the association of hMG...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(97)81407-0
更新日期:1997-05-01 00:00:00
abstract:OBJECTIVE:To conduct a pilot study to investigate the possible presence of bacteria throughout the female reproductive tract and to make a preliminary assessment of whether there are differences in the composition of the microbial communities between these body sites and/or between patients. DESIGN:Prospective pilot s...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2016.11.028
更新日期:2017-03-01 00:00:00
abstract:OBJECTIVE:To test the efficacy of endometrial preparation with exogenous steroids, without pretreatment with gonadotropin-releasing hormone (GnRH) agonist, in women with normal ovarian function. DESIGN:Prospective randomized study. SETTING:Private outpatient infertility clinic. PATIENT(S):Two hundred ninety-six wome...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0015-0282(02)02960-6
更新日期:2002-05-01 00:00:00
abstract::Under guidance of an external pulsed magnetic field the Cuproferrogel iron oxide-copper-styrene maleic anhydride-dimethyl sulphoxide delivered into the rat/rabbit oviduct resulted in oocytes with granulated cytoplasm, zona enlargement, membrane disintegration, and finally loss of viability in 72 hours. Also, the perce...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2010.06.032
更新日期:2010-12-01 00:00:00
abstract:OBJECTIVE:To assess the clinical relevance of daily hormonal changes for achieving a successful pregnancy in anovulatory infertile women. DESIGN:A comparative study of hormonal dynamics in pregnant and nonpregnant cycles during the pulsatile subcutaneous administration of hMG. Subjects received subcutaneous injection ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)56093-2
更新日期:1993-08-01 00:00:00
abstract:OBJECTIVE:To determine whether concurrent use of GnRH agonists with chemotherapy preserves ovarian function in women with breast cancer who did not use tamoxifen. DESIGN:Systematic review and meta-analysis. SETTING:University-based hospitals. PATIENT(S):Premenopausal women with breast cancer treated with chemotherap...
journal_title:Fertility and sterility
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.fertnstert.2014.06.003
更新日期:2014-09-01 00:00:00
abstract:OBJECTIVE:To evaluate the influence of the opioid system on the hypothalamic-pituitary-adrenal axis in women with polycystic ovary syndrome (PCOS). DESIGN:Controlled clinical study. SETTING:Academic research environment. PATIENT(S):Eight lean and 12 obese women with PCOS, and seven lean and 5 obese control subjects....
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章
doi:10.1016/s0015-0282(99)00602-0
更新日期:2000-04-01 00:00:00
abstract::Financial compensation to egg donors for their time and inconvenience in donating is established in the United States as ethically and legally acceptable. That system should be extended to egg donation for research. ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2006.08.084
更新日期:2006-12-01 00:00:00
abstract:OBJECTIVE:To explore the effectiveness of percutaneous vasal sperm aspiration (PVSA) in combination with intrauterine insemination (IUI) to treat infertile men with anejaculation. DESIGN:Clinical study. SETTING:Department of reproductive endocrinology and andrology of a family planning research clinic. PATIENT(S):Tw...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(02)04697-6
更新日期:2003-03-01 00:00:00
abstract::The third annual meeting of the Androgen Excess Society, held in San Diego, California, June 3, 2005, reported on new developments in the field, including intrafollicular steroidogenesis, intrathecal biochemistry, mechanisms of insulin signal and resistance of polycystic ovary syndrome, and novel aspects of androgen s...
journal_title:Fertility and sterility
pub_type:
doi:10.1016/j.fertnstert.2006.06.049
更新日期:2006-11-01 00:00:00