Abstract:
OBJECTIVE:To determine whether altered follicular environment is associated with ovarian reserve or maternal age. DESIGN:Prospective study examining follicular fluid (FF) composition and follicular cell metabolism. SETTING:University research department and private IVF clinic. PATIENT(S):Women (n = 54) undergoing routine IVF treatment were allocated to one of three groups based on ovarian reserve and maternal age. INTERVENTION(S):Surplus FF, granulosa cells (GC), and cumulus cells (CC) were collected. MAIN OUTCOME MEASURE(S):Follicular fluid concentrations of carbohydrates, hormones, and selected ions. Metabolic analysis and gene expression of GCs and CCs. RESULT(S):Compared to women <35 years with normal ovarian reserve, FF glucose levels were significantly decreased and lactate and progesterone (P4) concentrations significantly increased in women with reduced ovarian reserve or advanced maternal age, whereas GC and CC glucose uptake, lactate production, and phosphofructokinase platelet gene expression were significantly increased. Granulosa cell P4 production from women with reduced ovarian reserve or advanced maternal age was decreased; however, in CCs the reverse was observed with increased gene expression in P4 receptor, prostaglandin E receptor-2, cytosolic phospholipase A2, and tumor protein 53. CONCLUSION(S):Women with either reduced ovarian reserve or advanced maternal age have altered follicular cell metabolism, FF metabolites, and P4 production. This perturbed environment may be responsible for impaired oocyte developmental competence and subsequent embryo development.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Pacella L,Zander-Fox DL,Armstrong DT,Lane Mdoi
10.1016/j.fertnstert.2012.06.025subject
Has Abstractpub_date
2012-10-01 00:00:00pages
986-94.e1-2issue
4eissn
0015-0282issn
1556-5653pii
S0015-0282(12)00678-4journal_volume
98pub_type
杂志文章abstract:OBJECTIVE:To determine the prevalence of elevated total and free T, and DHEAS, alone and in combination, in patients with polycystic ovary syndrome (PCOS). DESIGN:Cross-sectional analysis. SETTING:Tertiary care academic medical center. PATIENT(S):Seven hundred twenty patients diagnosed with PCOS according to the Nat...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2008.12.138
更新日期:2010-04-01 00:00:00
abstract:OBJECTIVE:To investigate the slope of linear regression of postevacuation serum hCG as an independent risk factor for postmolar gestational trophoblastic neoplasia (GTN). DESIGN:Multicenter retrospective cohort study. SETTING:Academic referral health care centers. PATIENT(S):All subjects with confirmed hydatidiform ...
journal_title:Fertility and sterility
pub_type: 杂志文章,多中心研究
doi:10.1016/j.fertnstert.2015.06.001
更新日期:2015-09-01 00:00:00
abstract::Under guidance of an external pulsed magnetic field the Cuproferrogel iron oxide-copper-styrene maleic anhydride-dimethyl sulphoxide delivered into the rat/rabbit oviduct resulted in oocytes with granulated cytoplasm, zona enlargement, membrane disintegration, and finally loss of viability in 72 hours. Also, the perce...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2010.06.032
更新日期:2010-12-01 00:00:00
abstract::In this study, both IVF-ET cycle and pregnancy outcome were compared with increases seen in P concentration during the 24-hour period from 12 hours before to 12 hours after hCG administration. A significantly higher PR (P < 0.001) was seen in women who had at least a threefold increase in P during this time period. Fu...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1994-11-01 00:00:00
abstract:OBJECTIVE:To develop a reliable, reproducible, and sensitive method for investigating gene-expression profiles from individual human oocytes. DESIGN:Five commercially available protocols were investigated for their efficiency to amplify messenger RNA (mRNA) from 54 single human oocytes. Protocols resulting in sufficie...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2015.10.025
更新日期:2016-02-01 00:00:00
abstract::Reportedly, gamete intrafallopian transfer and zygote intrafallopian transfer are successful methods in assisted conception. This pilot study describes the experiences and results of a recently developed technique of vaginal transcervical intrafallopian transfer. In a group of 38 women with unexplained infertility, oo...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)53704-2
更新日期:1990-08-01 00:00:00
abstract:OBJECTIVE:To review, evaluate, and synthesize current published reports assessing the value of abdominal myomectomy in infertile women and those desiring to preserve fertility potential. DATA IDENTIFICATION:Major studies dealing with myomectomy were identified through Medline Searches. STUDY SELECTION:Those papers re...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/s0015-0282(16)55128-0
更新日期:1992-07-01 00:00:00
abstract:OBJECTIVE:To evaluate tubal morphology, trophoblast proliferation, and inflammatory reaction in response to methotrexate (MTX) treatment of ectopic pregnancy (EP). DESIGN:Nonrandomized controlled study. SETTING:Academic hospital. PATIENTS:Archival specimens from 10 EP unsuccessfully treated with MTX and 10 cases pri...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章
doi:
更新日期:1996-04-01 00:00:00
abstract::The International Committee for Monitoring Assisted Reproductive Technology's 7th World Report for the year 2000 analyzes wide variations in live and multiple birth rates from 49 countries and six regions by type of assisted reproductive technology, age, number of embryos transferred, and multiple births. More than 46...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2006.01.011
更新日期:2006-06-01 00:00:00
abstract:OBJECTIVE:To understand the barriers that serodiscordant couples with human immunodeficiency virus (HIV) face in accessing services for risk reduction and infertility using assisted reproductive technology (ART). DESIGN:Two-arm cross-sectional telephone "secret shopper" study. SETTING:Infertility clinics designated b...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2017.11.039
更新日期:2018-03-01 00:00:00
abstract:OBJECTIVE:To compare the efficacy of a sequential step-up/step-down protocol with the conventional low dose step-up and the step-down regimens in patients with polycystic ovary syndrome (PCOS) undergoing IVF. DESIGN:Prospective randomized study. SETTING:Private fertility clinic. PATIENT(S):Two hundred twenty-five, 3...
journal_title:Fertility and sterility
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.fertnstert.2007.06.080
更新日期:2008-09-01 00:00:00
abstract::Websites for Society for Assisted Reproductive Technology-affiliated clinics fail to meet most American Medical Association health information guidelines. Professional organizations in reproductive medicine need to standardize the accuracy and appropriateness of online reproductive health information. ...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/j.fertnstert.2004.11.024
更新日期:2005-03-01 00:00:00
abstract:OBJECTIVES:To investigate the presence of Poly (ADP-ribose) polymerase (PARP) and evaluate its function in ejaculated spermatozoa. DESIGN:Experimental study. SETTING:Reproductive Research Center. PATIENT(S):Semen specimens from 18 healthy donors and 12 infertile males. INTERVENTION(S):Ejaculated spermatozoa were su...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2007.12.079
更新日期:2009-03-01 00:00:00
abstract::Twenty-seven patients underwent ultrasonographic and clinical monitoring of a spontaneous cycle so that human chorionic gonadotropin administration could be timed in an attempt to schedule the screening laparoscopy at the optimal time for oocyte aspiration. Inadequate ovarian access was encountered in only 2 of 26 lap...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)46871-8
更新日期:1983-03-01 00:00:00
abstract:OBJECTIVE:To evaluate the efficacy of discoid resection for the treatment of deep infiltrating endometriosis and whether it could be considered to be a valid alternative to the rectosigmoid segmental resection. DESIGN:Case-control study. SETTING:Departments of Obstetrics and Gynecology, Ospedale Sacro Cuore of Negrar...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2009.03.066
更新日期:2010-07-01 00:00:00
abstract:OBJECTIVE:To evaluate surgical outcome and long-term follow-up of conservative laparoscopic management of urinary tract endometriosis (UTE). DESIGN:Prospective study. SETTING:Tertiary-care university hospital. PATIENT(S):Women with laparoscopic diagnosis and histologic confirmation of urinary bladder or ureteral end...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章
doi:10.1016/j.fertnstert.2009.04.019
更新日期:2010-08-01 00:00:00
abstract:OBJECTIVE:To assess the hypothesis that pregnancy rates (PRs) after operative laparoscopy (Laparoscopy Group) for endometriosis treatment would be equal to or greater than diagnostic laparoscopy only (No Treatment Group), diagnostic laparoscopy with medical treatment (Medical Treatment Group), and laparotomy (Laparotom...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract:OBJECTIVE:To investigate the utility of random anti-Müllerian hormone (AMH) in assessing ovarian response among women with diminished ovarian reserve (DOR) diagnosed by elevated early follicular-phase FSH levels. DESIGN:Retrospective study. SETTING:Academic and academically affiliated assisted reproductive technology...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2011.03.071
更新日期:2011-06-01 00:00:00
abstract:OBJECTIVE:To investigate the expression kinetics of interleukin-1 receptors (IL-1R), receptor antagonist (IL-1RN), and monocyte chemotactic protein 1 (MCP-1) throughout early gestation in mice. DESIGN:Assessment of IL-1R, IL-1RN, and MCP-1 throughout early pregnancy. SETTING:Reproduction laboratory. ANIMAL(S):B6C3F1...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2014.01.011
更新日期:2014-04-01 00:00:00
abstract:OBJECTIVE:To harmonize the collection of nonsurgical clinical and epidemiologic data relevant to endometriosis research, allowing large-scale collaboration. DESIGN:An international collaboration involving 34 clinical/academic centers and three industry collaborators from 16 countries on five continents. SETTING:In 20...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/j.fertnstert.2014.07.1244
更新日期:2014-11-01 00:00:00
abstract::Fertilization, embryo development, and successful pregnancy can be achieved after transfer of embryos derived from brown oocytes at the same rate as embryos from morphologically normal oocytes. Therefore, brown oocytes are probably normal and this morphological criterion does not seem to be indicative of any adverse o...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2006.03.056
更新日期:2006-11-01 00:00:00
abstract:OBJECTIVE:To study the safety and efficacy of ovarian stimulation and oocyte cryopreservation as a method of fertility preservation in women with Turner syndrome (TS). DESIGN:Retrospective cohort study. SETTING:Reproductive medicine clinic. PATIENT(S):Seven women with TS who attended the clinic between 2011 and 2017...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2018.11.010
更新日期:2019-03-01 00:00:00
abstract:OBJECTIVE:To evaluate the phenotype, proliferative, and differentiation capacities in vitro of stromal cells derived from peritoneal, ovarian, and deeply infiltrating endometriosis. DESIGN:Experimental study using phase contrast microscopy, immunocytochemistry, and functional bioassays. SETTING:University-based labor...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2005.08.046
更新日期:2006-03-01 00:00:00
abstract:OBJECTIVE:To establish the relative frequency of aneuploidy in sperm from normal and abnormal subjects using dual color fluorescence in situ hybridization and probes for six different chromosomes. DESIGN:Semen from 33 normal males and a patient with a translocation was studied using dual color fluorescence in situ hyb...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)56896-4
更新日期:1994-08-01 00:00:00
abstract:OBJECTIVE:To assess whether heart rate variability (HRV) at rest and during sympathetic stimulation is disturbed in patients with different polycystic ovary syndrome (PCOS) phenotypes in comparison to healthy controls. DESIGN:Cross-sectional study. SETTING:University hospital. PATIENT(S):Thirty women with classic, a...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2012.08.049
更新日期:2013-01-01 00:00:00
abstract:OBJECTIVE:To compare sperm characteristics among: patients undergoing infertility evaluation, patients with male factor infertility (MFI), healthy sperm donors, and men with proven fertility; to examine the overlap of sperm characteristics in all four of these groups; and to identify good discriminators of fertility ve...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2005.08.024
更新日期:2006-03-01 00:00:00
abstract::Semen analyses were performed and serum and seminal plasma prolactin (PRL) concentrations were determined in 165 samples from 120 men seen with their wives because of infertility. The mean (+/- standard deviation) serum and seminal plasma PRL concentrations were 6.5 +/- 3.3 and 7.5 +/- 3.1 ng/ml, respectively. The mea...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)59664-2
更新日期:1988-01-01 00:00:00
abstract::The association of insulin resistance, hyperandrogenism, and acanthosis nigricans is well established. The ovary is thought to be the site of the excess androgen production. The purpose of this study was to assess the adrenal contribution to the hyperandrogenic state in these women. Twenty-three patients presenting wi...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)50001-6
更新日期:1987-02-01 00:00:00
abstract:OBJECTIVE:To evaluate prospectively the effects of high-dose, short-term treatment with a glucocorticoid in an attempt to normalize ovarian failure and induce pregnancy in women presenting with infertility. DESIGN:Uncontrolled, nonrandomized prospective study. SETTING:Two university-based reproductive endocrinology c...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)55915-9
更新日期:1993-05-01 00:00:00
abstract:OBJECTIVE:To present a report on assisted reproductive technologies (ART) cycles performed in 2005 in Canada. This is the fifth annual report from the Canadian ART Register (CARTR). DESIGN:Prospective cohort study. SETTING:Twenty-five of 25 ART centers in Canada. PARTICIPANT(S):Couples undergoing ART treatment in Ca...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2008.02.125
更新日期:2009-05-01 00:00:00