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Factors associated with the quality before freezing and after thawing of sperm obtained by microsurgical epididymal aspiration.

Abstract：

OBJECTIVE:To identify whether the cause, site of ductal obstruction, and characteristics of fluid aspirates are associated with the cryosurvival and fertility after thawing of sperm obtained during reconstruction of the excurrent ducts with microsurgical epididymal sperm aspiration, vasal sperm aspiration, or both. DESIGN:Prospective study. SETTING:Andrology center at a tertiary care institution. PATIENT(S):Men undergoing reconstruction of the excurrent duct and sperm aspiration (n = 42) or microsurgical epididymal sperm aspiration (n = 11). INTERVENTION(S):Sperm were tested for an association with the cause and site of obstruction. Fertilization and pregnancy rates after sperm aspiration and intracytoplasmic sperm injection (ICSI) were evaluated for fresh and frozen aspirates. MAIN OUTCOME MEASURE(S):Motile sperm count and percentage motility after thawing. RESULT(S):The motile sperm count before freezing was significantly higher in the caput epididymis than in the corpus. The motile sperm count before freezing was related inversely to the distance from the caput where the sperm were aspirated. Sperm from clear and opaque fluid aspirates had better percent motility than those from cloudy and creamy fluid aspirates. High fertilization and pregnancy rates were achieved using both fresh and frozen epididymal sperm. CONCLUSION(S):None of the factors studied was associated with cryosurvival of aspirated epididymal or vasal spermatozoa. Because motility is low after thawing, these specimens are best used with ICSI.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Sharma RK,Padron OF,Thomas AJ Jr,Agarwal A

doi

10.1016/s0015-0282(97)00274-4

subject

Has Abstract

pub_date

1997-10-01 00:00:00

pages

626-31

issue

4

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(97)00274-4

journal_volume

68

pub_type

杂志文章

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