Abstract：
PURPOSE:The purpose of this study was to evaluate the Tono-Pen 2 tonometer for measuring intraocular pressure (IOP) in the living rat eye. METHODS:One eye from each of 20 adult, anesthetized brown Norway rats (group 1) was cannulated and simultaneously connected to a syringe and a pressure transducer with a chart recorder. We increased IOP from 15 to 45 mmHg in 5-mmHg increments and obtained 15 consecutive readings (ignoring instrument-generated averages) at each pressure increment with a Tono-Pen 2 tonometer. To test the tonopen's ability to measure unknown IOP, transducer pressures were varied randomly in 20 additional animals (group 2), and tonopen readings were obtained in masked fashion. RESULTS:Plotting the mean tonopen readings for each animal against transducer IOP produced a regression formula of y = 4.54 + 0.79x (r = 0.98). Mean group 2 tonopen values plotted against transducer IOP yielded a regression formula of y = 4.75 + 0.78x (r = 0.94). A method comparison analysis showed that the tonopen significantly overestimates pressures at low IOP (< or = 15 mmHg), and it significantly underestimates pressures at high IOP (> or = 30 mmHg). Using two-way analysis of variance, it was determined that the group 2 data did not differ significantly from the group 1 data (P > or = 0.76). Because of this consistency, we generated a correction factor with 95% prediction intervals for Tono-Pen readings. CONCLUSIONS:The Tono-Pen 2 can be used reliably to measure IOP in the normal rat eye.
journal_name
Invest Ophthalmol Vis Scijournal_title
Investigative ophthalmology & visual scienceauthors
Moore CG,Milne ST,Morrison JCsubject
Has Abstractpub_date
1993-02-01 00:00:00pages
363-9issue
2eissn
0146-0404issn
1552-5783journal_volume
34pub_type
杂志文章abstract：:Substance P (SP)--like immunoreactivity has been localized to distinct retinal cell populations in the primate Macaca nemestrina by means of immunohistochemical techniques with a well-characterized monoclonal antiserum directed to SP. The specificity of the immunoreactive staining was established by absorption of the ...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1982-08-01 00:00:00
abstract：Purpose:The purpose of this study was to investigate the impact of stanniocalcin-1 (STC-1), a photoreceptor-protective glycoprotein, on the development of choroidal neovascularization (CNV) in relation to VEGF and its main receptor (VEGFR2) expression after laser injury. Methods:In rats, CNV was induced by laser photo...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.17-23083
更新日期：2018-02-01 00:00:00
abstract：PURPOSE:To study the spatial distribution of posterior pole choroidal thickness (CT) in healthy eyes using spectral domain optical coherence tomography (SD-OCT). METHODS:Fifty-nine eyes from 30 subjects with no retinal or choroidal disease were examined with high-definition (HD) OCT using macular volume cube scanning ...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.11-8046
更新日期：2011-09-01 00:00:00
abstract：:Spleen cells of BALB/c mice, previously immunized with bovine retinal S-antigen, were fused with Sp2/0-Ag14 mouse myeloma cells. Two monoclonal antibodies (MAbs) MAbA9-C6 (IgG2a isotype) and MAbA1-G5 (IgG1 isotype) were selected on the basis of reactivity in an ELISA and immunofluorescent assay. In radioimmunoassay MA...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1985-04-01 00:00:00
abstract：PURPOSE:To investigate the effect of the tissue origin of stromal fibroblasts and epithelial cells on reconstructed corneas in vitro. METHODS:Four types of constructs were produced by the self-assembly approach using the following combinations of human cells: corneal fibroblasts/corneal epithelial cells, corneal fibro...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.08-2001
更新日期：2009-06-01 00:00:00
abstract：PURPOSE:Environmental tobacco smoke (ETS) is widely regarded as a major modifiable risk factor for age-related macular degeneration (AMD). Yet, precisely how it exerts its pathologic effects is poorly understood. Since early-stage AMD is characterized by choroidal capillary loss, this study examined the effect of sides...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.13-13659
更新日期：2014-04-08 00:00:00
abstract：PURPOSE:Methylenetetrahydrofolate reductase (Mthfr) is a key enzyme in homocysteine-methionine metabolism. We investigated Mthfr expression in retina and asked whether mild hyperhomocysteinemia, due to Mthfr deficiency, alters retinal neurovascular structure and function. METHODS:Expression of Mthfr was investigated a...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.14-16190
更新日期：2015-04-01 00:00:00
abstract：:Parallel studies of the ionic balance and membrane permeability characteristics of normal human lenses were carried out in three countries (USA, England and Italy). Similar age-related changes were found in each laboratory. The lens membrane potential and resistance declined markedly with age while internal Na+ and fr...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1989-08-01 00:00:00
abstract：Purpose:The purpose of this study was to investigate the disease-causing mutations for retinitis pigmentosa (RP) patients and function of mutations. Methods:We recruited RP families and sporadic RP patients, and performed whole-exome sequencing (WES) to screen for sequence variations. Subsequently, we investigated the...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.17-22952
更新日期：2017-12-01 00:00:00
abstract：PURPOSE:The authors describe a method for estimating the spheric aberration (SA) of the human crystalline lens in vivo. METHODS:This method was based on a comparison of the measured change in contrast sensitivity (delta log (CSF)) of the eye and the calculated change in modulation transfer function of the cornea with ...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1993-03-01 00:00:00
abstract：:We tested the state of retinal correspondence at different positions in the visual field of ten observers with strabismic and/or anisometropic amblyopia, four strabismic subjects with alternating fixation and three normal controls. Correspondence was evaluated by the subjective displacement of dichoptic stimuli; to es...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1989-09-01 00:00:00
abstract：PURPOSE:This study sought to determine the earliest time-point at which evidence of choroidal neovascularization (CNV) could be detected with visible-light optical coherence tomography angiography (vis-OCTA) in a mouse model of laser-induced CNV. METHODS:Visible light-OCTA was used to study laser-induced CNV at differ...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.15-18891
更新日期：2016-07-01 00:00:00
abstract：PURPOSE:To determine the mechanism by which basic fibroblast growth factor (bFGF) exerts its neuroprotective effects on degenerating or injured photoreceptors. METHODS:Confocal immunofluorescence microscopy was used to identify sites of bFGF and FGF receptor 1 (FGFR1) expression after focal injury or experimental reti...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：2000-02-01 00:00:00
abstract：PURPOSE:To compare accommodative facility in eyes with myopia to that in eyes with emmetropia or hyperopia and to determine whether accommodative facility can be used to predict an association with myopia. METHODS:In the Sydney Myopia Study, year-1 school children (6.7 +/- 0.4 years) were assessed for accommodative fa...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章,多中心研究
doi：10.1167/iovs.05-1078
更新日期：2006-11-01 00:00:00
abstract：PURPOSE:To compare conventional pattern-reversal visual evoked potentials (cVEPs) with multifocal VEPs (mfVEPs). METHODS:mfVEPs and cVEPs were recorded during the same session in 12 normal subjects with an active electrode at Oz referenced to the inion (Oz-In) or to a midfrontal position, Fz (Oz-Fz). The mfVEP stimulu...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.02-0441
更新日期：2003-03-01 00:00:00
abstract：PURPOSE:Optical coherence tomography (OCT) has been used to investigate papilledema in single-site, mostly retrospective studies. We investigated whether spectral-domain OCT (SD-OCT), which provides thickness and volume measurements of the optic nerve head and retina, could reliably demonstrate structural changes due t...
journal_title：Investigative ophthalmology & visual science
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1167/iovs.14-14960
更新日期：2014-11-04 00:00:00
abstract：:We use a population balance model to study the mechanism and the rate of centripetal migration of epithelial cells, renewal of the corneal epithelial population by the cell derived from (progeny of) the limbal stem cells and the kinetics of the replacement of the donor corneal epithelium. The epithelial mass is consta...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1989-09-01 00:00:00
abstract：:We describe a new technique for the separation and isolation of nonpigmented ciliary body epithelial cells from rabbit. Excised ciliary body is incubated in a medium buffered with EGTA to a free-Ca2+ concentration of 10(-8) M, and the nonpigmented cell layer is separated from the pigmented layer by microdissection und...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1988-05-01 00:00:00
abstract：:Fibroblasts in the retroocular connective tissue appear to play a central role in the pathogenesis of Graves' ophthalmopathy (GO). We hypothesize that specific attributes, differentiating normal from GO retroocular fibroblasts, may render the latter more vulnerable to the ongoing immunopathological process in GO. We i...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1992-02-01 00:00:00
abstract：PURPOSE:To characterize the prejunctional mechanisms that control the impulse-evoked release of norepinephrine in the isolated, superfused human iris-ciliary body. METHODS:Human iris-ciliary body tissue segments were preincubated with 3H-norepinephrine, superfused and electrically-stimulated in vitro to evoke the disc...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1993-08-01 00:00:00
abstract：:Mucins, which play important roles on the ocular surface in wettability, lubrication, and barrier function, are classified into two categories: secreted mucins and membrane-associated mucins. The most important secreted mucin on the ocular surface is MUC5AC, which is secreted by the conjunctival goblet cells. In the h...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章,评审
doi：10.1167/iovs.17-23623
更新日期：2018-11-01 00:00:00
abstract：Purpose:The cone-rod homeobox (CRX) transcription factor is essential for photoreceptor gene expression, differentiation, and survival. Human CRX mutations can cause dominant retinopathies of varying onset and phenotype severity. In animal models, dominant frameshift Crx mutations introduce a premature termination codo...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.17-22075
更新日期：2017-09-01 00:00:00
abstract：:Refractile inclusion bodies (approximately 0.80 micron in size) were found in the inner segments of cone photoreceptors in the aging human retina. They were easily resolved with the light microscope. They were never seen in rods, and occurred primarily in retinas from eye donors older than 40 years of age. The inciden...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1986-05-01 00:00:00
abstract：PURPOSE:The purpose of this study was to investigate tumor control efficacy of paclitaxel in the treatment of retinal tumors harbored by the LH beta-Tag murine transgenic model of retinoblastoma. METHODS:LH beta-Tag-positive mice (n = 5) 10 weeks of age received two 20-microL subconjunctival injections delivered with ...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.06-0796
更新日期：2007-08-01 00:00:00
abstract：PURPOSE:To test the effect of valacyclovir alone and with aspirin on the asymptomatic shedding of HSV-1 DNA in tears and saliva of healthy individuals. METHOD. The subjects (n = 45) were randomized into three groups without regard to age, sex, or race. Group 1 (n = 14) received the placebo, group 2 (n = 15) received a ...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章,随机对照试验
doi：10.1167/iovs.09-3729
更新日期：2009-12-01 00:00:00
abstract：Purpose:To investigate the action of atropine on global flash multifocal electroretinogram (gmfERG) responses to retinal defocus. Method:gmfERG recordings were made monocularly in 19 healthy adults under three lens-imposed defocus conditions (2 diopters myopic, 2 diopters hyperopic, and no defocus) before and 24 hours...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.18-24600
更新日期：2019-01-02 00:00:00
abstract：PURPOSE:To compare EyeCam (Clarity Medical Systems, Pleasanton, CA) imaging with gonioscopy for detecting angle closure. METHODS:In this prospective, hospital-based study, subjects underwent gonioscopy by a single observer and EyeCam imaging by a different operator. EyeCam images were graded by two masked observers. T...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.09-4418
更新日期：2010-06-01 00:00:00
abstract：PURPOSE:To determine the effect of bimatoprost on intraocular pressure in the prostaglandin FP receptor knockout mouse. METHODS:The IOP response to a single 1.2-microg (4 microL) dose of bimatoprost was measured in the treated and untreated fellow eyes of homozygote (FP+/+, n = 9) and heterozygote (FP+/-, n = 10) FP-k...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.05-0723
更新日期：2005-12-01 00:00:00
abstract：PURPOSE:To investigate the function of connective tissue growth factor (CTGF), a matricellular protein of the CCN (Cyr61/CTGF/Nov) family, in retinal vasculature during development and ischemia. METHODS:CTGF expression was determined using RT-PCR, immunohistochemistry, and transgenic mice carrying CTGF promoter-driven...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.11-7870
更新日期：2011-11-07 00:00:00
abstract：:PURPOSE. To evaluate the shape of the posterior corneal surface in eyes with normal myopia or myopic astigmatism and the changes after LASIK and epi-LASIK. METHODS. A total of 152 eyes were included in the study. The posterior corneal elevation was measured with Scheimpflug imaging (Pentacam; Oculus, Wetzlar, Germany)...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.09-4811
更新日期：2010-07-01 00:00:00
