Role of connective tissue growth factor in the retinal vasculature during development and ischemia.

abstract：PURPOSE:To explore the maximum retinal vasodilation in response to repeated flicker light stimulation in relation to vasospastic propensity in healthy subjects. METHODS:Twenty-four young healthy women were grouped as vasospastic and nonvasospastic, based on their history of cold extremities and on the results of nailf...

journal_title：Investigative ophthalmology & visual science

authors： Gugleta K,Zawinka C,Rickenbacher I,Kochkorov A,Katamay R,Flammer J,Orgul S

更新日期：2006-09-01 00:00:00

abstract：PURPOSE:This study focuses on the identification of regulatory elements that contribute to lens-specific expression of the human CP49 gene within the 5'-flanking DNA sequences. METHODS:The DNA sequence upstream of the human CP49 coding region was subcloned as a set of 5' and 3' deletion series. The constructs were tra...

journal_title：Investigative ophthalmology & visual science

authors： DePianto DJ,Hess JF,Blankenship TN,FitzGerald PG

更新日期：2003-01-01 00:00:00

abstract：PURPOSE:To investigate the patterns of retinal nerve fiber layer (RNFL) defects in mean deviation-matched early glaucomatous eyes with either superior or inferior visual hemifield loss. METHODS:Seventy-five open-angle glaucoma patients with isolated parafoveal scotoma (PFS) within a central 10° of fixation, and 62 pat...

journal_title：Investigative ophthalmology & visual science

authors： Choi JA,Park HY,Jung KI,Hong KH,Park CK

更新日期：2013-10-23 00:00:00

abstract：PURPOSE:Genetic modification of cells before transplantation may allow the delivery of neuroprotective and other functional molecules to patients with neurodegenerative diseases. To avoid complications associated with virally transfected cells, we have explored the use of nonviral methods to insert genetic material int...

journal_title：Investigative ophthalmology & visual science

authors： Johnen S,Kazanskaya O,Armogan N,Stickelmann C,Stöcker M,Walter P,Thumann G

更新日期：2011-07-23 00:00:00

abstract：:Extracellular matrix (ECM) turnover and remodeling are initiated, at least in part, by the regulated secretion of members of a family of matrix metalloproteinases. Human and bovine trabecular mesh-work in culture secrete interstitial collagenase, both the 72- and the 92-kD forms of type IV collagenase (gelatinases) an...

journal_title：Investigative ophthalmology & visual science

authors： Alexander JP,Samples JR,Van Buskirk EM,Acott TS

更新日期：1991-01-01 00:00:00

abstract：PURPOSE:To address the consequences of visual deprivation paradigms in rat (dark rearing) and monkey (monocular deprivation) on extraocular muscle (EOM) development using genome-wide expression profiling. METHODS:Serial analysis of gene expression (SAGE) was used to determine alterations in the EOM transcriptome induc...

journal_title：Investigative ophthalmology & visual science

authors： Cheng G,Mustari MJ,Khanna S,Porter JD

更新日期：2003-09-01 00:00:00

abstract：PURPOSE:Docosahexaenoic acid (DHA) was supplemented in a single-site, placebo-controlled, randomized clinical trial designed to slow vision loss associated with X-linked retinitis pigmentosa (XLRP); the DHAX Trial. We previously reported no significant differences between supplemented and placebo groups in intent-to-tr...

journal_title：Investigative ophthalmology & visual science

authors： Hoffman DR,Hughbanks-Wheaton DK,Spencer R,Fish GE,Pearson NS,Wang YZ,Klein M,Takacs A,Locke KG,Birch DG

更新日期：2015-10-01 00:00:00

abstract：:Intact rod photoreceptors were dissociated from pronase-treated whole retinas of adult mice by repeated passage through a plastic pipette tip. Hemocytometer counts of the cell suspensions indicate that, during a series of ten dissociation steps, a total of about 1-2 million intact photoreceptor cells are dissociated f...

journal_title：Investigative ophthalmology & visual science

authors： Lolley RN,Lee RH,Chase DG,Racz E

更新日期：1986-03-01 00:00:00

abstract：PURPOSE:To define rod and cone function further in terms of visual cycle mechanism, the retinal phenotype resulting from Rpe65 (retinoid isomerase I) deficiency in Nrl(-)(/)(-) mice having a single class of photoreceptors resembling wild-type cones was characterized and outcomes of retinoid supplementation evaluated. ...

journal_title：Investigative ophthalmology & visual science

authors： Feathers KL,Lyubarsky AL,Khan NW,Teofilo K,Swaroop A,Williams DS,Pugh EN Jr,Thompson DA

更新日期：2008-03-01 00:00:00

abstract：PURPOSE:Activation of complement has been implicated as one of the major causes of age-related macular degeneration (AMD). Evidence is accumulating for a role of complement in other retinal diseases, such as diabetic retinopathy and proliferative vitreoretinopathy. Because of the paucity of animal models that directly ...

journal_title：Investigative ophthalmology & visual science

authors： Cashman SM,Desai A,Ramo K,Kumar-Singh R

更新日期：2011-05-18 00:00:00

abstract：:Congenic mice of the MRL/Mp strain spontaneously develop an autoimmune connective tissue disease that shares immunologic and histopathologic features with the human disorders systemic lupus erythematosus, rheumatoid arthritis, and systemic vasculitis. The autoimmune disorder in these mice is markedly accelerated by th...

journal_title：Investigative ophthalmology & visual science

authors： Jabs DA,Alexander EL,Green WR

更新日期：1985-09-01 00:00:00

abstract：PURPOSE:To investigate the neuroprotective effects of brain-derived neurotrophic factor (BDNF) against potassium cyanide (KCN)-induced retinal damage. METHODS:Rats were injected intravitreally with iodinated BDNF. Two days later, eyeballs were dissected into various parts, and the level of radioactivity in each part w...

journal_title：Investigative ophthalmology & visual science

authors： Ikeda K,Tanihara H,Honda Y,Tatsuno T,Noguchi H,Nakayama C

更新日期：1999-08-01 00:00:00

abstract：PURPOSE:The goal of this study was to functionally evaluate three previously uncharacterized heat shock factor protein 4 (HSF4) mutations (c.595_599delGGGCC, c.1213C>T, c.1327+4A>G) encoding mutant HSF4 proteins (G199EfsX15, R405X, and M419GfsX29) with missing C-terminal ends. These HSF4 mutations were previously ident...

journal_title：Investigative ophthalmology & visual science

authors： Merath K,Ronchetti A,Sidjanin DJ

更新日期：2013-10-11 00:00:00

abstract：PURPOSE:Müller cells are the principal glial cells of the retina. They span the entire thickness of the neural retina, and they are in close contact with neurons. Müller cells grow very slowly, and they undergo senescence with increasing passages. Moreover, successful primary cultures of Müller cells can be obtained on...

journal_title：Investigative ophthalmology & visual science

authors： Lupien CB,Salesse C

更新日期：2007-02-01 00:00:00

abstract：Purpose:To determine whether mitochondrial DNA haplogroups or rare variants associate with primary open-angle glaucoma in subjects of European descent. Methods:A case-control comparison of age- and sex-matched cohorts of 90 primary open-angle glaucoma patients and 95 population controls. Full mitochondrial DNA sequenc...

journal_title：Investigative ophthalmology & visual science

authors： Singh LN,Crowston JG,Lopez Sanchez MIG,Van Bergen NJ,Kearns LS,Hewitt AW,Yazar S,Mackey DA,Wallace DC,Trounce IA

更新日期：2018-09-04 00:00:00

abstract：PURPOSE:Age-related macular degeneration (AMD), the most common cause of incurable blindness in the western world, is characterized by the dysfunction and eventual death of choroidal endothelial (CECs), RPE, and photoreceptor cells. Stem cell-based treatment strategies designed to replace photoreceptor and RPE cells cu...

journal_title：Investigative ophthalmology & visual science

authors： Songstad AE,Wiley LA,Duong K,Kaalberg E,Flamme-Wiese MJ,Cranston CM,Riker MJ,Levasseur D,Stone EM,Mullins RF,Tucker BA

更新日期：2015-12-01 00:00:00

abstract：PURPOSE:Intraocular pressure (IOP) is dynamic and can be influenced by the use of tonometers. The authors developed a technique to implant a telemetric pressure transducer to measure IOP continuously in unrestrained rabbits. METHODS:A commercial telemetric pressure transducer was implanted subcutaneously on the dorsal...

journal_title：Investigative ophthalmology & visual science

authors： McLaren JW,Brubaker RF,FitzSimon JS

更新日期：1996-05-01 00:00:00

abstract：Purpose:The purpose of this paper was to discuss manifestations of primary mitochondrial dysfunctions and whether the retinal pigment epithelium or the photoreceptors are preferentially affected. Methods:A retrospective analysis was performed of patients with clinically and laboratory confirmed diagnoses of maternally...

journal_title：Investigative ophthalmology & visual science

authors： Oh JK,Lima de Carvalho JR Jr,Nuzbrokh Y,Ryu J,Chemudupati T,Mahajan VB,Sparrow JR,Tsang SH

更新日期：2020-10-01 00:00:00

abstract：PURPOSE:Macular pigment (MP) may protect against age-related macular degeneration. This study was conducted to determine the extent of changes in the macular pigment density as a consequence of oral supplementation with lutein. A second purpose was to compare two objective measurement techniques. METHODS:In the first ...

journal_title：Investigative ophthalmology & visual science

authors： Berendschot TT,Goldbohm RA,Klöpping WA,van de Kraats J,van Norel J,van Norren D

更新日期：2000-10-01 00:00:00

abstract：:Glaucoma has been known to be associated with a loss of retinal ganglion cells and their axons throughout the fundus and a decreased count of photoreceptors and retinal pigment epithelial (RPE) cells in the parapapillary region. This study investigated whether glaucomatous changes of the deep retinal layer occur outsi...

journal_title：Investigative ophthalmology & visual science

authors： Panda S,Jonas JB

更新日期：1992-07-01 00:00:00

abstract：PURPOSE:To prepare binary transgenic mouse lines that overexpress reporter genes in a corneal-epithelium-specific manner when induced by doxycycline. METHODS:A gene-targeting construct containing an internal ribosomal entry site-reverse tetracycline transcription activator (IRES-rtTA) cassette was inserted into the Kr...

journal_title：Investigative ophthalmology & visual science

authors： Chikama T,Hayashi Y,Liu CY,Terai N,Terai K,Kao CW,Wang L,Hayashi M,Nishida T,Sanford P,Doestchman T,Kao WW

更新日期：2005-06-01 00:00:00

abstract：Purpose:To improve the transparency of the acellular dermal matrix (ADM) and investigate the optical, mechanical and histologic properties and biocompatibility of transparent ADM (TADM) in lamellar keratoplasty. Methods:A stepwise sectioning strategy was applied to determine the transparency distribution of the ADM, a...

journal_title：Investigative ophthalmology & visual science

authors： Wang Y,Ma J,Jiang X,Liu Z,Yang J,Li X

更新日期：2020-01-23 00:00:00

abstract：PURPOSE:Familial exudative vitreoretinopathy (FEVR) is a rare hereditary disorder characterized by the failure of peripheral retinal vascularization. The genes FZD4, LRP5, and TSPAN12 are known to be associated with the autosomal inheritance form of FEVR. In this study, we performed mutation screening for FZD4, LRP5, a...

journal_title：Investigative ophthalmology & visual science

authors： Seo SH,Yu YS,Park SW,Kim JH,Kim HK,Cho SI,Park H,Lee SJ,Seong MW,Park SS,Kim JY

更新日期：2015-08-01 00:00:00

abstract：PURPOSE:Fundamental to the maintenance of ionic concentration gradients and transparency of the lens is the activity of Na+,K+-adenosine triphosphatase (ATPase) in the epithelial layer. Recent studies have identified endogenous digitalis-like compounds (DLCs) and 19-norbufalin and its peptide derivatives in human catar...

journal_title：Investigative ophthalmology & visual science

authors： Lichtstein D,Levy T,Deutsch J,Steinitz M,Zigler JS Jr,Russell P

更新日期：1999-02-01 00:00:00

abstract：PURPOSE:To investigate mechanical properties of the human posterior lens capsule. METHODS:Twenty-five human donor eyes were obtained from an eye bank. The age of the donors ranged from 1 to 94 years. Test specimens were prepared as tissue rings from posterior lens capsules by means of excimer laser. Capsular thickness...

journal_title：Investigative ophthalmology & visual science

authors： Krag S,Andreassen TT

更新日期：2003-02-01 00:00:00

abstract：PURPOSE:To improve the interpretation of Heidelberg Retina Flowmeter (HRF; Heidelberg Engineering GmBH, Dosselheim, Germany) flow maps by examining the influence of specific vascular structures and focus depth in the presence and absence of retinal blood flow. METHODS:HRF flow maps were recorded from the inferior reti...

journal_title：Investigative ophthalmology & visual science

authors： Yu DY,Townsend R,Cringle SJ,Chauhan BC,Morgan WH

更新日期：2005-01-01 00:00:00

abstract：PURPOSE:To identify proteinases and growth factors abnormally expressed in human corneas of donors with diabetic retinopathy (DR), additional to previously described matrix metalloproteinase (MMP)-10 and -3 and insulin-like growth factor (IGF)-I. METHODS:RNA was isolated from 35 normal, diabetic, and DR autopsy human ...

journal_title：Investigative ophthalmology & visual science

authors： Saghizadeh M,Kramerov AA,Tajbakhsh J,Aoki AM,Wang C,Chai NN,Ljubimova JY,Sasaki T,Sosne G,Carlson MR,Nelson SF,Ljubimov AV

更新日期：2005-10-01 00:00:00

abstract：PURPOSE:Although the murine alphaA-crystallin promoter is the most commonly used promoter for achieving transgene expression in the developing lens, this promoter directs transgene expression efficiently only in lens fiber cells. The purpose of the present study was to generate promoters capable of directing transgene ...

journal_title：Investigative ophthalmology & visual science

authors： Zhao H,Yang Y,Rizo CM,Overbeek PA,Robinson ML

更新日期：2004-06-01 00:00:00

abstract：PURPOSE:Human tear film stability decreases with increasing age. In this study, the changes in meibum composition were measured in search of markers of tear film instability. METHODS:(1)H NMR nuclear magnetic resonance (NMR) spectra of 43 normal donors aged 1 to 88 years were acquired. RESULTS:Compared with meibum fr...

journal_title：Investigative ophthalmology & visual science

authors： Borchman D,Foulks GN,Yappert MC,Milliner SE

更新日期：2012-01-31 00:00:00

abstract：:The amount of unscheduled DNA synthesis (UDS) produced in the cells adjacent to excimer laser ablations in the cornea was compared for 193-nm and 248-nm laser wavelengths. UDS is interpreted to indicate the process of excision repair of pyrimidine dimers formed in DNA. 193-nm laser ablation did not produce a statistic...

journal_title：Investigative ophthalmology & visual science

authors： Nuss RC,Puliafito CA,Dehm E

更新日期：1987-02-01 00:00:00