Unscheduled DNA synthesis following excimer laser ablation of the cornea in vivo.

Abstract:

:The amount of unscheduled DNA synthesis (UDS) produced in the cells adjacent to excimer laser ablations in the cornea was compared for 193-nm and 248-nm laser wavelengths. UDS is interpreted to indicate the process of excision repair of pyrimidine dimers formed in DNA. 193-nm laser ablation did not produce a statistically significant difference in the amount of UDS as compared to a negative control (diamond-knife corneal incision). However, 248-nm laser ablation did produce a highly statistically significant difference in the amount of UDS as compared to both the negative control (P < 0.001) and the 193-nm laser irradiation (P < 0.001). Other forms of DNA damage (single-strand DNA chain breaks and DNA-protein crosslinks) are not measurable by UDS and need to be investigated in the evaluation of the oncogenic potential of 193-nm laser ablation.

authors

Nuss RC,Puliafito CA,Dehm E

subject

Has Abstract

pub_date

1987-02-01 00:00:00

pages

287-94

issue

2

eissn

0146-0404

issn

1552-5783

journal_volume

28

pub_type

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