Unscheduled DNA synthesis following excimer laser ablation of the cornea in vivo.

Abstract:

:The amount of unscheduled DNA synthesis (UDS) produced in the cells adjacent to excimer laser ablations in the cornea was compared for 193-nm and 248-nm laser wavelengths. UDS is interpreted to indicate the process of excision repair of pyrimidine dimers formed in DNA. 193-nm laser ablation did not produce a statistically significant difference in the amount of UDS as compared to a negative control (diamond-knife corneal incision). However, 248-nm laser ablation did produce a highly statistically significant difference in the amount of UDS as compared to both the negative control (P < 0.001) and the 193-nm laser irradiation (P < 0.001). Other forms of DNA damage (single-strand DNA chain breaks and DNA-protein crosslinks) are not measurable by UDS and need to be investigated in the evaluation of the oncogenic potential of 193-nm laser ablation.

authors

Nuss RC,Puliafito CA,Dehm E

subject

Has Abstract

pub_date

1987-02-01 00:00:00

pages

287-94

issue

2

eissn

0146-0404

issn

1552-5783

journal_volume

28

pub_type

杂志文章
  • Characterization of effector T cells in dry eye disease.

    abstract:PURPOSE:Dry eye disease (DED) is associated with ocular surface inflammation that is thought to be mediated primarily by CD4 T cells. The purpose of this study was to investigate whether this T cell-mediated immune response is generated in the lymphoid compartment and to characterize the functional phenotype of the T c...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.08-2417

    authors: El Annan J,Chauhan SK,Ecoiffier T,Zhang Q,Saban DR,Dana R

    更新日期:2009-08-01 00:00:00

  • Videographic Hirschberg measurement of simulated strabismic deviations.

    abstract:PURPOSE:To demonstrate the potential use of subpixel image processing methods to perform automated Hirschberg measurements of strabismic deviations using relatively inexpensive personal computer hardware; to determine if the method might allow screening for strabismus using full-face video images obtained from a distan...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Miller JM,Mellinger M,Greivenkemp J,Simons K

    更新日期:1993-10-01 00:00:00

  • Role of exogenous and endogenous trophic factors in the regulation of extraocular muscle strength during development.

    abstract:PURPOSE:Weak eye muscles can cause ophthalmic disorders, and in particular, strabismus. Exogenous trophic factors such as cardiotrophin (CT)-1 and insulin-like growth factor (IGF) have been shown to increase the contractile force of adult heart and skeletal muscles, respectively. In the current study, the effects of en...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.04-0393

    authors: Chen J,von Bartheld CS

    更新日期:2004-10-01 00:00:00

  • The effect of complete tenotomy on blood flow to the anterior segment of the canine eye.

    abstract::The relative importance of the anterior ciliary arteries to blood flow to the anterior segment and the quadrant distribution of blood to the iris, ciliary body, and ciliary processes were determined in canines with the use of 15 +/- 3 mum 103Ru microspheres with the reference blood flow method. Recti muscles were isol...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Keough EM,Wilcox LM,Connolly RJ,Hotte CE

    更新日期:1980-11-01 00:00:00

  • A practical method for measuring macular pigment optical density.

    abstract:PURPOSE:Increasing evidence indicates that the macular pigments (MP) protect the central retina and may retard macular disease. For that reason, a practical method for measuring MP that does not require elaborate optics and can be applied to diverse populations by operators with a modest amount of experience was develo...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Wooten BR,Hammond BR Jr,Land RI,Snodderly DM

    更新日期:1999-10-01 00:00:00

  • Feasibility of Macular Integrity Assessment (MAIA) Microperimetry in Children: Sensitivity, Reliability, and Fixation Stability in Healthy Observers.

    abstract:Purpose:To assess the feasibility of macular integrity assessment (MAIA) microperimetry (MP) in children. Also to establish representative outcome measures (differential light sensitivity, fixation stability, test-retest reliability) for children without visual impairment. Methods:Thirty-three adults and 33 children (...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.16-20037

    authors: Jones PR,Yasoubi N,Nardini M,Rubin GS

    更新日期:2016-11-01 00:00:00

  • Three-Dimensional Segmentation and Quantitative Measurement of the Aqueous Outflow System of Intact Mouse Eyes Based on Spectral Two-Photon Microscopy Techniques.

    abstract:PURPOSE:To visualize and quantify the three-dimensional (3D) spatial relationships of the structures of the aqueous outflow system (AOS) within intact enucleated mouse eyes using spectral two-photon microscopy (TPM) techniques. METHODS:Spectral TPM, including two-photon autofluorescence (TPAF) and second-harmonic gene...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.15-18491

    authors: Zhang X,Liu N,Mak PU,Pun SH,Vai MI,Masihzadeh O,Kahook MY,Lei TC,Ammar DA

    更新日期:2016-06-01 00:00:00

  • Safety profile of topical VEGF neutralization at the cornea.

    abstract:PURPOSE:Bevacizumab eyedrops inhibit corneal neovascularization. The purpose of this study was to analyze the safety profile of VEGF-A neutralization at the ocular surface. METHODS:Bevacizumab eyedrops (5 mg/mL) and an antimurine VEGF-A antibody (250 microg/mL) were applied to normal murine corneas five times a day fo...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.07-1129

    authors: Bock F,Onderka J,Rummelt C,Dietrich T,Bachmann B,Kruse FE,Schlötzer-Schrehardt U,Cursiefen C

    更新日期:2009-05-01 00:00:00

  • Activation of protein tyrosine phosphorylation after retinal branch vein occlusion in cats.

    abstract:PURPOSE:The authors examine the effect of retinal branch vein occlusion (BVO), a common retinal vascular disorder, on protein tyrosine phosphorylation, production of angiogenic growth factors, and activation of signal proteins in the tyrosine kinase pathways in the retina. METHODS:Retinal branch vein occlusion was ind...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Hayashi A,Imai K,Kim HC,de Juan E Jr

    更新日期:1997-02-01 00:00:00

  • Prevalence, incidence, and progression of myopia of school children in Hong Kong.

    abstract:PURPOSE:To determine the prevalence, incidence, and progression of myopia of Chinese children in Hong Kong. METHODS:A cross-sectional survey was initially conducted. A longitudinal follow-up study was then conducted 12 months later. RESULTS:A total of 7560 children of mean age 9.33 (95% confidence interval [CI] = 9.1...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.03-1151

    authors: Fan DS,Lam DS,Lam RF,Lau JT,Chong KS,Cheung EY,Lai RY,Chew SJ

    更新日期:2004-04-01 00:00:00

  • Growth factor localization in choroidal neovascular membranes of age-related macular degeneration.

    abstract:PURPOSE:Because several polypeptide growth factors are known to influence capillary endothelial cell mitogenesis, the authors investigated the presence of some of these molecules in choroidal neovascular membranes (CNVMs) removed surgically from human subjects with age-related macular degeneration (ARMD). METHODS:The ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Amin R,Puklin JE,Frank RN

    更新日期:1994-07-01 00:00:00

  • Inhibition of DNA and RNA synthesis in lymphocyte cultures by rod outer segments and its counteraction by vitamin E and other antioxidants.

    abstract::Bovine rod outer segments (ROS) specifically stimulate DNA synthesis in lymphocyte cultures from guinea pigs preimmunized with ROS. At doses above 10 microgram/ml protein, however, preparations of ROS inhibit DNA and RNA synthesis by the sensitized lymphocytes or by normal lymphocytes reacting to mitogens. The inhibti...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Gery I

    更新日期:1980-07-01 00:00:00

  • The novel Rho kinase (ROCK) inhibitor K-115: a new candidate drug for neuroprotective treatment in glaucoma.

    abstract:PURPOSE:To investigate the effect of K-115, a novel Rho kinase (ROCK) inhibitor, on retinal ganglion cell (RGC) survival in an optic nerve crush (NC) model. Additionally, to determine the details of the mechanism of K-115's neuroprotective effect in vivo and in vitro. METHODS:ROCK inhibitors, including K-115 and fasud...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.13-13842

    authors: Yamamoto K,Maruyama K,Himori N,Omodaka K,Yokoyama Y,Shiga Y,Morin R,Nakazawa T

    更新日期:2014-10-02 00:00:00

  • Polymorphisms of the DNA repair genes XPD and XRCC1 and the risk of age-related macular degeneration.

    abstract:PURPOSE:Oxidative stress seems to be an important factor in the development of age-related macular degeneration (AMD). The role of DNA repair mechanisms has also received attention recently in AMD pathogenesis. This case-control study was conducted to determine the frequency of polymorphisms in two DNA repair enzyme ge...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.09-4842

    authors: Görgün E,Güven M,Unal M,Batar B,Güven GS,Yenerel M,Tatlipinar S,Seven M,Yüksel A

    更新日期:2010-09-01 00:00:00

  • Experimental percutaneous cannulation of the supraorbital arteries: implication for future therapy.

    abstract:PURPOSE:There is some evidence to suggest that thrombolysis has a beneficial effect in retinal vessel occlusion. However, there is concern that this therapeutic approach carries the risk of hemorrhage. Retrograde cannulation of the supraorbital arteries followed by irrigation with fibrinolytic agents may have the poten...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.04-1129

    authors: Schwenn OK,Wüstenberg EG,Konerding MA,Hattenbach LO

    更新日期:2005-05-01 00:00:00

  • Effect of aminoguanidine, a nitric oxide synthase inhibitor, on ocular infection with herpes simplex virus in Balb/c mice.

    abstract:PURPOSE:To study the effect of aminoguanidine (AMG), an inhibitor of nitric oxide production, on the ocular infection of Balb/c mice with herpes simplex virus (HSV) type 1 strain F and HSV-2 strain G. METHODS:Animals were treated with different amounts of AMG (0.5, 0.1, and 0.05 mg/mouse) by topical application in the...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Benencia F,Courrèges MC,Gamba G,Cavalieri H,Massouh EJ

    更新日期:2001-05-01 00:00:00

  • Influences of atmospheric pressure and temperature on intraocular pressure.

    abstract:PURPOSE:To determine whether the atmospheric pressure (ATM) change experienced during diving can induce changes in the intraocular pressure (IOP) of eyes in a normal population. METHODS:The IOP of 27 healthy volunteers (ages, 23.8 +/- 4.9 years; range, 18-44) was measured with a Perkins applanation tonometer by two in...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.07-1578

    authors: Van de Veire S,Germonpre P,Renier C,Stalmans I,Zeyen T

    更新日期:2008-12-01 00:00:00

  • Effects of bone marrow mesenchymal stem cell transplantation on light-damaged retina.

    abstract::PURPOSE. To investigate the effects and possible mechanisms of rat bone marrow mesenchymal stem cell (BMSC) transplantation on the light-damaged retinal structure and the apoptosis of photoreceptors. METHODS. DAPI-labeled BMSCs were transplanted into the subretinal space of light-damaged Sprague-Dawley rats 10 days af...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.08-3314

    authors: Zhang Y,Wang W

    更新日期:2010-07-01 00:00:00

  • Paradoxic effect of anti-CD4 therapy on lacrimal gland disease in MRL/Mp-lpr/lpr mice.

    abstract:PURPOSE:MRL/Mp-lpr/lpr mice (MRL/lpr) spontaneously develop lacrimal gland inflammatory lesions and are a model for the human disease Sjögren's syndrome. Therapy with monoclonal antibodies (mAb) to CD4 ameliorates the autoimmune renal, vasculitic, and intraocular inflammatory lesions in MRL/lpr mice. The effect of anti...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Jabs DA,Burns WH,Prendergast RA

    更新日期:1996-01-01 00:00:00

  • Different patterns of retinal correspondence in the central and peripheral visual field of strabismics.

    abstract::We tested the state of retinal correspondence at different positions in the visual field of ten observers with strabismic and/or anisometropic amblyopia, four strabismic subjects with alternating fixation and three normal controls. Correspondence was evaluated by the subjective displacement of dichoptic stimuli; to es...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Sireteanu R,Fronius M

    更新日期:1989-09-01 00:00:00

  • Human CRB1-associated retinal degeneration: comparison with the rd8 Crb1-mutant mouse model.

    abstract:PURPOSE:To investigate the human disease due to CRB1 mutations and compare results with the Crb1-mutant rd8 mouse. METHODS:Twenty-two patients with CRB1 mutations were studied. Function was assessed with perimetry and electroretinography (ERG) and retinal structure with optical coherence tomography (OCT). Cortical str...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.11-7701

    authors: Aleman TS,Cideciyan AV,Aguirre GK,Huang WC,Mullins CL,Roman AJ,Sumaroka A,Olivares MB,Tsai FF,Schwartz SB,Vandenberghe LH,Limberis MP,Stone EM,Bell P,Wilson JM,Jacobson SG

    更新日期:2011-08-29 00:00:00

  • Transient Intraocular Pressure Fluctuations: Source, Magnitude, Frequency, and Associated Mechanical Energy.

    abstract:Purpose:To characterize intraocular pressure (IOP) dynamics by identifying the sources of transient IOP fluctuations and quantifying the frequency, magnitude, associated cumulative IOP-related mechanical energy, and temporal distribution. Methods:IOP was monitored at 500 Hz for periods of 16 to 451 days in nine normal...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.19-26600

    authors: Turner DC,Edmiston AM,Zohner YE,Byrne KJ,Seigfreid WP,Girkin CA,Morris JS,Downs JC

    更新日期:2019-06-03 00:00:00

  • Adaptive changes in dynamic properties of human disparity-induced vergence.

    abstract:PURPOSE:Vergence eye movements undergo adaptive recalibration in response to a training stimulus in which the initial disparity is changed just after vergence begins (the double-step paradigm). In the present study the changes in the dynamic properties of convergence, speed and acceleration, were examined by using this...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Takagi M,Oyamada H,Abe H,Zee DS,Hasebe H,Miki A,Usui T,Hasegawa S,Bando T

    更新日期:2001-06-01 00:00:00

  • Tissue Transglutaminase Elevates Intraocular Pressure in Mice.

    abstract:Purpose:Tissue transglutaminase (TGM2) is elevated in glaucomatous trabecular meshwork (TM) tissues. We investigated whether increased expression of TGM2 increases extracellular matrix crosslinking in the TM, thereby increasing aqueous humor outflow resistance and elevating intraocular pressure (IOP) in mouse eyes. Me...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.17-22236

    authors: Raychaudhuri U,Millar JC,Clark AF

    更新日期:2017-12-01 00:00:00

  • Nrl-knockout mice deficient in Rpe65 fail to synthesize 11-cis retinal and cone outer segments.

    abstract:PURPOSE:To define rod and cone function further in terms of visual cycle mechanism, the retinal phenotype resulting from Rpe65 (retinoid isomerase I) deficiency in Nrl(-)(/)(-) mice having a single class of photoreceptors resembling wild-type cones was characterized and outcomes of retinoid supplementation evaluated. ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.07-1234

    authors: Feathers KL,Lyubarsky AL,Khan NW,Teofilo K,Swaroop A,Williams DS,Pugh EN Jr,Thompson DA

    更新日期:2008-03-01 00:00:00

  • Murine model of ocular infection by a human biovar of Chlamydia trachomatis.

    abstract:PURPOSE:A human biovar of Chlamydia trachomatis was used to develop a murine model of ocular chlamydial infection. The inbred mouse model will allow detailed immunologic studies during ocular infection, and use of a human biovar for infection may aid in identification of appropriate vaccine strategies against chlamydia...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Whittum-Hudson JA,O'Brien TP,Prendergast RA

    更新日期:1995-09-01 00:00:00

  • Corneal Epithelial Remodeling Induced by Small Incision Lenticule Extraction (SMILE).

    abstract:PURPOSE:To assess the reactive changes of the corneal epithelial thickness (ET) profile induced by small incision lenticule extraction (SMILE) for treatment of myopia. METHODS:This prospective observational study included 46 eyes of 23 patients that were scheduled for myopic SMILE. High-resolution spectral-domain opti...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.15-18879

    authors: Luft N,Ring MH,Dirisamer M,Mursch-Edlmayr AS,Kreutzer TC,Pretzl J,Bolz M,Priglinger SG

    更新日期:2016-07-01 00:00:00

  • Apoptotic retinal cell death induced by antirecoverin autoantibodies of cancer-associated retinopathy.

    abstract:PURPOSE:Recoverin has been identified as a target autoantigen for antirecoverin antibodies found in the sera of some patients with cancer-associated retinopathy. The aim of this study was to investigate the role of antirecoverin antibodies in cancer-associated retinopathy. METHODS:Human, rat, and rabbit antirecoverin ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Adamus G,Machnicki M,Seigel GM

    更新日期:1997-02-01 00:00:00

  • Early detection of central visual function decline in cone-rod dystrophy by the use of macular focal cone electroretinogram.

    abstract:PURPOSE:To evaluate macular focal cone ERG (fERG) as a tool for reliable and early detection of central retinal function decay in cone-rod dystrophy (CRD). METHODS:A retrospective study of the time course of fERG amplitude and its relation to visual acuity alterations was performed in 47 CRD patients followed yearly f...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.13-12676

    authors: Galli-Resta L,Piccardi M,Ziccardi L,Fadda A,Minnella A,Marangoni D,Placidi G,Resta G,Falsini B

    更新日期:2013-10-09 00:00:00

  • Localization of the site of major resistance to fluid transport in Bruch's membrane.

    abstract:PURPOSE:To determine the site of highest resistance to the movement of water across Bruch's membrane in humans. METHODS:A hydraulic conductivity chamber was designed that enabled us to measure flow across Bruch's membrane while ablating its subepithelial aspect using an excimer laser (193 nm). When resistance was lost...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Starita C,Hussain AA,Patmore A,Marshall J

    更新日期:1997-03-01 00:00:00