Characterization of tetracycline-inducible bitransgenic Krt12rtTA/+/tet-O-LacZ mice.

Abstract:

PURPOSE:To prepare binary transgenic mouse lines that overexpress reporter genes in a corneal-epithelium-specific manner when induced by doxycycline. METHODS:A gene-targeting construct containing an internal ribosomal entry site-reverse tetracycline transcription activator (IRES-rtTA) cassette was inserted into the Krt12 allele (keratin 12 gene) to produce a knock-in Krt12(rtTA/+) mouse line through gene-targeting techniques. The Krt12(rtTA/+) knock-in mice were bred with tet-O-LacZ reporter mice to obtain Krt12(rtTA/+)/tet-O-LacZ bitransgenic mice. The expression of the LacZ gene was induced in bitransgenic mice by administration of doxycycline in the drinking water and chow. RESULTS:Administration of doxycycline induced a 15-fold increase of beta-galactosidase activity in the cornea of adult bitransgenic mice (Krt12(rtTA/+)/tet-O-lacZ). Administration of doxycycline either to single transgenic Krt12(rtTA/+) or tet-O-LacZ mice as a control did not induce overexpression of LacZ as it did in the bitransgenic mice. The induction of beta-galactosidase enzyme activity by doxycycline in bitransgenic mice took place in 24 hours and reached a plateau by 2 days. Histochemical analysis also showed that beta-galactosidase induction was limited to the corneal epithelium of bitransgenic mice fed doxycycline. The increased beta-galactosidase activity in corneal epithelium caused by doxycycline returned to basal levels in 4 weeks after the antibiotics were omitted from the diet. CONCLUSIONS:A binary mouse model has been successfully established that conditionally overexpresses reporter genes in corneal epithelium. This mouse model will be useful in elucidating signaling pathways of various growth factors and cytokines and gene functions in the maintenance of homeostasis and pathogenesis in the adult mouse cornea.

authors

Chikama T,Hayashi Y,Liu CY,Terai N,Terai K,Kao CW,Wang L,Hayashi M,Nishida T,Sanford P,Doestchman T,Kao WW

doi

10.1167/iovs.04-1464

keywords:

subject

Has Abstract

pub_date

2005-06-01 00:00:00

pages

1966-72

issue

6

eissn

0146-0404

issn

1552-5783

pii

46/6/1966

journal_volume

46

pub_type

杂志文章
  • Peripapillary choroidal thickness variation with age and race in normal eyes.

    abstract:PURPOSE:This study examined the association between peripapillary choroidal thickness (PCT) with age and race in a group of African descent (AD) and European descent (ED) subjects with normal eyes. METHODS:Optic nerve head images from enhanced depth imaging spectral-domain optical coherence tomography of 166 normal ey...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.14-16179

    authors: Rhodes LA,Huisingh C,Johnstone J,Fazio MA,Smith B,Wang L,Clark M,Downs JC,Owsley C,Girard MJ,Mari JM,Girkin CA

    更新日期:2015-02-24 00:00:00

  • Effect of iontophoretic and topical application of antiviral agents in treatment of experimental HSV-1 keratitis in rabbits.

    abstract::Cathodal (-) iontophoresis of 9-beta-D-arabinofuranosyl-adenine 5'-monophosphate (vidarabine monophosphate; Ara-AMP) was performed once daily for 3 days for the treatment of experimental herpes simplex virus type 1 (HSV-1) keratitis in rabbit eyes, and the therapeutic efficacy was compared with that of topical treatme...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Kwon BS,Gangarosa LP,Park NH,Hull DS,Fineberg E,Wiggins C,Hill JM

    更新日期:1979-09-01 00:00:00

  • Eye movements in schizophrenia.

    abstract::Smooth pursuit and other eye movements were quantitatively studied in patients with chronic schizophrenia to characterize the pattern and severity of eye movement abnormalities in this disorder. Twenty-one patients with schizophrenia, 13 patients with other psychiatric disorders (manic-depressive psychosis, schizoaffe...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Yee RD,Baloh RW,Marder SR,Levy DL,Sakala SM,Honrubia V

    更新日期:1987-02-01 00:00:00

  • Variations in eye volume, surface area, and shape with refractive error in young children by magnetic resonance imaging analysis.

    abstract:PURPOSE:To determine variations in eye volume, surface area, and shape with refractive error in young children using a three-dimensional magnetic resonance imaging (MRI) model. METHODS:A subset of Singaporean Chinese boys enrolled in the population-based Strabismus, Amblyopia, and Refractive Error in Singapore (STARS)...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.11-7269

    authors: Lim LS,Yang X,Gazzard G,Lin X,Sng C,Saw SM,Qiu A

    更新日期:2011-11-17 00:00:00

  • Three-dimensional modeling of metabolic species transport in the cornea with a hydrogel intrastromal inlay.

    abstract:PURPOSE:Intrastromal inlays for refractive correction of presbyopia are being adopted into clinical practice. An important concern is the effect of the inlay on the long-term health of the cornea due to disturbances in the concentration profiles of metabolic species. A three-dimensional metabolic model for the cornea i...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.13-13844

    authors: Pinsky PM

    更新日期:2014-05-15 00:00:00

  • The Effect of Atropine on Human Global Flash mfERG Responses to Retinal Defocus.

    abstract:Purpose:To investigate the action of atropine on global flash multifocal electroretinogram (gmfERG) responses to retinal defocus. Method:gmfERG recordings were made monocularly in 19 healthy adults under three lens-imposed defocus conditions (2 diopters myopic, 2 diopters hyperopic, and no defocus) before and 24 hours...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.18-24600

    authors: Khanal S,Turnbull PRK,Lee N,Phillips JR

    更新日期:2019-01-02 00:00:00

  • Effect of lipid peroxidation inhibition on retinal ganglion cell death.

    abstract:PURPOSE:To determine whether the lipid peroxidation inhibitor tirilazad mesylate can block the death of retinal ganglion cells induced by the inhibition of oxidative phosphorylation and glycolysis. METHODS:Rat retinal ganglion cells were labeled retrogradely with the fluorescent tracer DiI, and mixed cultures were pre...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Levin LA,Clark JA,Johns LK

    更新日期:1996-12-01 00:00:00

  • Change in full-field ERGs after macular translocation surgery with 360 degrees retinotomy.

    abstract:PURPOSE:One of the methods used in macular translocation (MT) surgery for subfoveal neovascularization is to create a temporary total retinal detachment followed by a 360 degrees retinotomy. The whole retina is then shifted from the original surface of the retinal pigment epithelium (RPE), resulting in an unusual retin...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Terasaki H,Miyake Y,Suzuki T,Niwa T,Piao CH,Suzuki S,Nakamura M,Kondo M

    更新日期:2002-02-01 00:00:00

  • Mathematical analysis of specific anatomic foveal configurations predisposing to the formation of macular holes.

    abstract:PURPOSE:To mathematically analyze and to clinically describe specific anatomic foveal configurations predisposing to the formation of macular holes in comparison with normal foveal anatomy. METHODS:In a retrospective observational case-control series, a total of 3882 optical coherence tomography (OCT) foveal thickness...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.11-8191

    authors: Barak Y,Sherman MP,Schaal S

    更新日期:2011-10-21 00:00:00

  • Transiently raised intraocular pressure reveals pattern electroretinogram losses in ocular hypertension.

    abstract:PURPOSE:It is known that the pattern electroretinogram (PERG) of normal subjects is reduced temporarily after short-term elevation of intraocular pressure (IOP). The authors evaluated PERG changes in ocular hypertension during and after transient IOP elevation. METHODS:Steady state (8 Hz) PERGs to sinusoidal gratings ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Colotto A,Falsini B,Salgarello T,Buzzonetti L,Cermola S,Porrello G

    更新日期:1996-12-01 00:00:00

  • Intraocular pressure of rhesus monkey (Macaca mulatta). I. An initial survey of two free-breeding colonies.

    abstract::As part of an ophthalmic and anthropometric survey of two free-breeding rhesus monkey (Macaca mulatta) populations, intraocular pressure (IOP) of 114 animals was measured under ketamine catalepsia with the use of a floating-tip pneumatonometer. The mean IOP of the 102 animals included in the main analyses was 14.9 +/-...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Bito LZ,Merritt SQ,DeRousseau CJ

    更新日期:1979-08-01 00:00:00

  • Long-term evaluation of refractive status and optical components in eyes of children born prematurely.

    abstract:PURPOSE:To evaluate the refractive status and optical components in school age children born prematurely and to examine the risk factors associated with refractive errors. METHODS:The participants were a cohort of children aged 7 to 9 years with gestational age less than 35 weeks or birth weight less than 1500 g. The ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.10-5234

    authors: Chen TC,Tsai TH,Shih YF,Yeh PT,Yang CH,Hu FC,Lin LL,Yang CM

    更新日期:2010-12-01 00:00:00

  • MMP14 Cleavage of VEGFR1 in the Cornea Leads to a VEGF-Trap Antiangiogenic Effect.

    abstract:PURPOSE:To determine the possible antiangiogenic effect of metalloproteinase (MMP) 14 cleavage of vascular endothelial growth factor receptor 1 (VEGFR1) in the cornea. METHODS:Recombinant mouse (rm) VEGFR1 was incubated with various concentrations of recombinant MMP14 to examine proteolysis in vitro. The reaction mixt...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.14-16248

    authors: Han KY,Dugas-Ford J,Lee H,Chang JH,Azar DT

    更新日期:2015-08-01 00:00:00

  • Central corneal thickness and thickness of the lamina cribrosa in human eyes.

    abstract:PURPOSE:Since central corneal thickness may inversely influence the amount and rate of progression of glaucomatous optic nerve damage and because lamina cribrosa thickness may be of importance in susceptibility to glaucoma, it was the purpose of the present study to evaluate whether central corneal thickness is related...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.04-0851

    authors: Jonas JB,Holbach L

    更新日期:2005-04-01 00:00:00

  • Interferon-gamma inhibits collagen synthesis by human Tenon's capsule fibroblasts in vitro.

    abstract::Collagen deposition is largely responsible for scar information, an undesirable outcome of glaucoma filtering surgery. Because interferon-gamma (IFN-gamma) has been reported to inhibit collagen synthesis in a variety of cells, the authors examined its effect on collagen synthesis by Tenon's capsule fibroblasts culture...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Low SQ,Kitada S,Lee DA

    更新日期:1991-10-01 00:00:00

  • Pyrophosphorolysis detects B-RAF mutations in primary uveal melanoma.

    abstract:PURPOSE:Mutations in the genes that control cell proliferation in cutaneous melanoma are generally uncommon in uveal melanoma. Despite the absence of known activating mutations, the RAF-MEK-ERK, or mitogen-activated protein kinase (MAPK), pathway is usually activated in uveal melanoma. An assay with increased potential...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.07-0722

    authors: Maat W,Kilic E,Luyten GP,de Klein A,Jager MJ,Gruis NA,Van der Velden PA

    更新日期:2008-01-01 00:00:00

  • Neuropeptide Y and the retinal pigment epithelium: receptor subtypes, signaling, and bioelectrical responses.

    abstract:PURPOSE:To characterize the potential for neuropeptide Y (NPY) signaling in the retinal pigment epithelium (RPE) by identifying the NPY receptor subtypes present, determining the effect of NPY on second-messenger production and membrane conductance, and establishing the neural retina as a site of NPY gene expression. ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Ammar DA,Hughes BA,Thompson DA

    更新日期:1998-09-01 00:00:00

  • Long-Term Biomechanical and Histologic Results of WST-D/NIR Corneal Stiffening in Rabbits, Up to 8 Months Follow-up.

    abstract:Purpose:To determine the long-term safety and efficacy of WST-D/near-infrared (NIR) corneal stiffening. Methods:One eye of 23 New Zealand White rabbits was de-epithelialized mechanically followed by topical application of 2.5 mg/mL WST11, combined with dextran-500 (WST-D) for 20 minutes. Subsequently, samples were irr...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.17-22108

    authors: Brekelmans J,Goz A,Dickman MM,Brandis A,Sui X,Wagner HD,Nuijts RMMA,Scherz A,Marcovich AL

    更新日期:2017-08-01 00:00:00

  • Transplantation of tissue-engineered epithelial cell sheets after excimer laser photoablation reduces postoperative corneal haze.

    abstract:PURPOSE:To apply tissue-engineered cell sheet transplantation after excimer laser keratectomy as a novel approach for the reduction of postoperative corneal haze. METHODS:Limbal biopsy specimens were obtained, and epithelial cells were cultured on temperature-responsive culture inserts without the use of feeder cells....

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.05-0995

    authors: Hayashida Y,Nishida K,Yamato M,Yang J,Sugiyama H,Watanabe K,Hori Y,Maeda N,Kikuchi A,Okano T,Tano Y

    更新日期:2006-02-01 00:00:00

  • Identification of novel mutations in the carbohydrate sulfotransferase gene (CHST6) causing macular corneal dystrophy.

    abstract:PURPOSE:Macular corneal dystrophy (MCD) is a rare corneal dystrophy that is characterized by abnormal deposits in the corneal stroma, keratocytes, Descemet's membrane, and endothelium, accompanied by progressive clouding. It has been classified into three immunophenotypes--MCD types I, IA, and II--according to the seru...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: El-Ashry MF,Abd El-Aziz MM,Wilkins S,Cheetham ME,Wilkie SE,Hardcastle AJ,Halford S,Bayoumi AY,Ficker LA,Tuft S,Bhattacharya SS,Ebenezer ND

    更新日期:2002-02-01 00:00:00

  • Assessment of the Retina of Plp-α-Syn Mice as a Model for Studying Synuclein-Dependent Diseases.

    abstract:Purpose:Synucleinopathies such as multiple system atrophy (MSA) and Parkinson's disease are associated with a variety of visual symptoms. Functional and morphological retinal aberrations are therefore supposed to be valuable biomarkers for these neurodegenerative diseases. This study examined the retinal morphology and...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.61.6.12

    authors: Kaehler K,Seitter H,Sandbichler AM,Tschugg B,Obermair GJ,Stefanova N,Koschak A

    更新日期:2020-06-03 00:00:00

  • Genetic evidence for role of carotenoids in age-related macular degeneration in the Carotenoids in Age-Related Eye Disease Study (CAREDS).

    abstract:PURPOSE:We tested variants in genes related to lutein and zeaxanthin status for association with age-related macular degeneration (AMD) in the Carotenoids in Age-Related Eye Disease Study (CAREDS). METHODS:Of 2005 CAREDS participants, 1663 were graded for AMD from fundus photography and genotyped for 424 single nucleo...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章,多中心研究

    doi:10.1167/iovs.13-13216

    authors: Meyers KJ,Mares JA,Igo RP Jr,Truitt B,Liu Z,Millen AE,Klein M,Johnson EJ,Engelman CD,Karki CK,Blodi B,Gehrs K,Tinker L,Wallace R,Robinson J,LeBlanc ES,Sarto G,Bernstein PS,SanGiovanni JP,Iyengar SK

    更新日期:2014-01-29 00:00:00

  • Osteopontin expression and serum levels in metastatic uveal melanoma: a pilot study.

    abstract:PURPOSE:This was a pilot study conducted to examine the expression of osteopontin in uveal melanoma and to determine whether serum osteopontin can be used in detecting metastatic uveal melanoma. METHODS:Osteopontin mRNA was measured in three uveal melanoma cell lines of various invasive potential by real-time PCR. Tis...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.05-0422

    authors: Kadkol SS,Lin AY,Barak V,Kalickman I,Leach L,Valyi-Nagy K,Majumdar D,Setty S,Maniotis AJ,Folberg R,Pe'er J

    更新日期:2006-03-01 00:00:00

  • Intravascular presence of tumor cells as prognostic parameter in uveal melanoma: a 35-year survey.

    abstract:PURPOSE:Invasion of tumor cells into blood vessels is essential for metastasis of uveal melanoma. The occurrence of ingrowth of tumor cells in blood vessels in uveal melanoma was analyzed, and this parameter was compared with the survival of the patients. METHODS:Between 1972 and 2007, 643 eyes primarily enucleated fo...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.09-3824

    authors: Ly LV,Odish OF,Wolff-Rouendaal Dd,Missotten GS,Luyten GP,Jager MJ

    更新日期:2010-02-01 00:00:00

  • Linkage analysis of quantitative refraction and refractive errors in the Beaver Dam Eye Study.

    abstract:PURPOSE:Refraction, as measured by spherical equivalent, is the need for an external lens to focus images on the retina. While genetic factors play an important role in the development of refractive errors, few susceptibility genes have been identified. However, several regions of linkage have been reported for myopia ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.10-7096

    authors: Klein AP,Duggal P,Lee KE,Cheng CY,Klein R,Bailey-Wilson JE,Klein BE

    更新日期:2011-07-13 00:00:00

  • Concurrent downregulation of a glutamate transporter and receptor in glaucoma.

    abstract:PURPOSE:Elevated levels of extracellular glutamate have been implicated in the pathophysiology of neuronal loss in both central nervous system and ophthalmic disorders, including glaucoma. This increase in glutamate may result from a failure of glutamate transporters, which are molecules that ordinarily regulate extrac...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Naskar R,Vorwerk CK,Dreyer EB

    更新日期:2000-06-01 00:00:00

  • Acuity recovery and cone pigment regeneration after a bleach in patients with retinitis pigmentosa and rhodopsin mutations.

    abstract:PURPOSE:To assess visual acuity recovery times and cone photopigment regeneration kinetics after a bleach in the fovea of patients with dominant retinitis pigmentosa due to rhodopsin mutations. METHODS:The authors measured acuity recovery times by computerized photostress testing in 13 patients with dominant retinitis...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Sandberg MA,Pawlyk BS,Berson EL

    更新日期:1999-09-01 00:00:00

  • Rectus extraocular muscle paths and decompression surgery for Graves orbitopathy: mechanism of motility disturbances.

    abstract:PURPOSE:To study possible causes of motility disturbances that may result from orbital decompression surgery in patients with Graves orbitopathy and especially the role of rectus extraocular muscle paths. METHODS:Sixteen patients with Graves orbitopathy were studied before and 3 to 6 months after translid (6 patients)...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Abràmoff MD,Kalmann R,de Graaf ME,Stilma JS,Mourits MP

    更新日期:2002-02-01 00:00:00

  • Long-term visual and refractive outcomes following surface ablation techniques in a large population for myopia correction.

    abstract:PURPOSE:To evaluate the visual and refractive outcome for four wavefront-guided surface ablation (WGSA) techniques (LASEK, LASEK flap-off [LASEK FO], Epi-LASIK, and Epi-LASIK flap-off [Epi-LASIK FO]) in a large myopic population. METHODS:This retrospective review included 1000 myopic eyes (spherical equivalent [SE] -1...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.12-10387

    authors: Kulkarni SV,AlMahmoud T,Priest D,Taylor SE,Mintsioulis G,Jackson WB

    更新日期:2013-01-21 00:00:00

  • Human corneal endothelial cells employ phosphorylation of p27(Kip1) at both Ser10 and Thr187 sites for FGF-2-mediated cell proliferation via PI 3-kinase.

    abstract:PURPOSE:FGF-2 stimulates cell proliferation of rabbit corneal endothelial cells (rCECs) by degrading the cyclin-dependent kinase inhibitor p27(Kip1) (p27) through its phosphorylation mechanism. The authors investigated whether the cell proliferation of human CECs (hCECs) is also induced by FGF-2 stimulation through the...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.11-8213

    authors: Lee JG,Song JS,Smith RE,Kay EP

    更新日期:2011-10-17 00:00:00