Abstract：
:Anchorage-dependent cells (NRK 49F) were grown at 37 degrees C in serum containing medium on glass coated with a N-isopropyl acrylamide/N-t-butyl acrylamide copolymer (NIPAaM/NTBAM) with a lower critical solution temperature (LCST) of 8 degrees C. After incubating the dishes and adherent cells at 4 degrees C for 1 h, the cells had started to detach in 'sheets', without the use of trypsin; by 2 h the cells had fully detached and formed aggregates. We presume that at 4 degrees C, below the LCST of the coating, the polymer swelled and eventually dissolved, leaving the cells unattached to the solid substrate. We speculate that the 'sheet' morphology of the detaching cells implies that the extracellular matrix produced by the cells during culture was left intact, which may then alter the nature of their subsequent behaviour. The polymer was not optimum for cell attachment and optimization would be needed to realize the potential of this novel mode of cell culture and recovery.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Rollason G,Davies JE,Sefton MVdoi
10.1016/0142-9612(93)90230-ysubject
Has Abstractpub_date
1993-01-01 00:00:00pages
153-5issue
2eissn
0142-9612issn
1878-5905pii
0142-9612(93)90230-Yjournal_volume
14pub_type
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