Abstract:
:The Ah receptor (AHR) is a basic helix-loop-helix protein that mediates the effects of 2,3,7,8-tetrachloro-dibenzo-p-dioxin. In this report, we describe a rabbit reticulocyte system that allows functional expression of both the AHR and its dimeric partner, the AHR nuclear translocator protein (ARNT). By using this in vitro system, we were able to reconstitute agonist binding to the AHR and agonist-induced AHR-ARNT recognition of a cognate DNA enhancer sequence. Expression of AHR deletion mutants revealed the location of N-terminal domains responsible for ligand and DNA recognition and C-terminal domains that play roles in agonist-induced DNA recognition.
journal_name
Proc Natl Acad Sci U S Aauthors
Dolwick KM,Swanson HI,Bradfield CAdoi
10.1073/pnas.90.18.8566subject
Has Abstractpub_date
1993-09-15 00:00:00pages
8566-70issue
18eissn
0027-8424issn
1091-6490journal_volume
90pub_type
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