Abstract:
:The production of collagen-degrading proteases by cultured neonatal rat microglia was examined using an immobilized fibronectin-gelatin matrix coupled to a fluorescent marker and by substrate gel analysis. When microglia were plated onto the surface of the matrix and incubated under resting (nonstimulated) conditions, a small but visible amount of immobilized matrix was degraded. Treatment with lipopolysaccharide (LPS) or interleukin-1 (IL-1) significantly increased the number of microglia demonstrating substrate degradation. Substrate-SDS polyacrylamide gel electrophoresis of samples of supernatants from untreated cultured microglia indicated the presence of a 72 and a 92 kD metalloproteinase with characteristics corresponding to collagenases. Supernatants from untreated astrocyte cultures were shown to have primarily a 72 kD metalloproteinase. Proteinase activity increased on stimulation of the microglia with LPS and IL-1 in a dose-dependent fashion. These results indicate that cultured microglia release active proteases capable of degrading the extracellular matrix in a localized region. The production of proteases by activated microglia may have important physiological and pathophysiological consequences within the restricted extracellular matrix of the CNS.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Colton CA,Keri JE,Chen WT,Monsky WLdoi
10.1002/jnr.490350309subject
Has Abstractpub_date
1993-06-15 00:00:00pages
297-304issue
3eissn
0360-4012issn
1097-4547journal_volume
35pub_type
杂志文章abstract::Adrenal chromaffin cell (ACC) transplants, alone or combined with levodopa treatment, were used in attempted therapy for Parkinson's disease (PD). In a previous study, we demonstrated that levodopa caused chromaffin cell death either by necrosis or by apoptosis in cell culture. Here we report the beneficial effect of ...
journal_title:Journal of neuroscience research
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journal_title:Journal of neuroscience research
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journal_title:Journal of neuroscience research
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journal_title:Journal of neuroscience research
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journal_title:Journal of neuroscience research
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journal_title:Journal of neuroscience research
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:
更新日期:1999-11-15 00:00:00
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journal_title:Journal of neuroscience research
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journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
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journal_title:Journal of neuroscience research
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journal_title:Journal of neuroscience research
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doi:10.1002/jnr.20681
更新日期:2005-12-15 00:00:00
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(sici)1097-4547(19970801)49:3<292::aid-jnr
更新日期:1997-08-01 00:00:00
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journal_title:Journal of neuroscience research
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更新日期:2012-10-01 00:00:00
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
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journal_title:Journal of neuroscience research
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doi:
更新日期:1997-04-15 00:00:00
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更新日期:1999-09-01 00:00:00
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
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更新日期:2006-08-15 00:00:00
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
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更新日期:2015-06-01 00:00:00