Abstract：
:The limited availability and potential to culture primary human brain cells means that there is still a need for cell lines that reliably model human neurons and glial cells. The human-derived NTera2/D1 (NT2) cell line is a promising tool from which both neuronal (NT2N) and astrocytic (NT2A) cells can be derived in vitro. Here we have investigated the potential to use this cell model to investigate the endocannabinoid system in the CNS. Through immunocytochemical characterization with a range of neuronal and glial markers, we found that these cell lines differentiate into cells with immature neuronal and astrocytic phenotypes, respectively. By real-time PCR, immunocytochemistry, and functional inhibition of cAMP accumulation, the cannabinoid 1 receptors were identified only on NT2N cells, consistent with high levels of expression of this receptor in neuronal cells of the CNS. No evidence of cannabinoid 2 receptor expression was found on any of the NT2 cell types. Both the precursors and the differentiated NT2N and NT2A cells demonstrated mRNA expression for the key enzymes involved in endocannabinoid synthesis and degradation. This work establishes a cannabinergic phenotype in NT2N and NT2A cells, providing an alternative human derived renewable cell model for investigation of cannabinoid receptor function and endocannabinoid synthesis and metabolism in the CNS.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Goodfellow CE,Graham SE,Dragunow M,Glass Mdoi
10.1002/jnr.22692subject
Has Abstractpub_date
2011-10-01 00:00:00pages
1685-97issue
10eissn
0360-4012issn
1097-4547journal_volume
89pub_type
杂志文章abstract：:The concept of the neurovascular unit emphasizes that common signals and substrates underlie the physiology and pathophysiology of neuronal and endothelial compartments in brain. Recent data suggest that activation of the integrin-associated protein CD47 promotes neuronal cell death. Is it possible that CD47 may also ...
journal_title：Journal of neuroscience research
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journal_title：Journal of neuroscience research
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490270321
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490360308
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journal_title：Journal of neuroscience research
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doi：10.1002/1097-4547(20010101)63:1<20::AID-JNR3>3.0.C
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pub_type： 杂志文章
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490260202
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journal_title：Journal of neuroscience research
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doi：10.1002/jnr.490380605
更新日期：1994-08-15 00:00:00
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pub_type： 杂志文章
doi：10.1002/jnr.22333
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490370208
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