Abstract:
:Empty major histocompatibility complex (MHC) class I molecules present on the surface of RMA-S (26 degrees C) cells were loaded with the iodinated peptides APGNYPAL, FAPGNYPAL (SEV-9) and RGYVYQGL (VSV-8), respectively. The thermostability of these peptide-loaded MHC class I molecules was assessed using temperature gradient native polyacrylamide gel electrophoresis. A linear temperature gradient perpendicular to the direction of electrophoresis yielded a graphical representation of the melting of MHC class I molecules. The class I signal disappeared when the peptide melted out of the groove, and gave rise to a second signal due to released peptide. APGNYPAL-loaded class I molecules melted at 11 degrees C with considerable release even at 0 degrees C. VSV-8-loaded class I molecules melted first at 36 degrees C, whereas SEV-9-loaded molecules melted at about 22 degrees C. A discrimination between the binding of SEV-9 to Kb and Db molecules was seen in the melting patterns. Results are discussed in correlation with known crystallographic structures of class I molecules containing peptides in the binding groove.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Tulp A,Verwoerd DJdoi
10.1002/eji.1830230847subject
Has Abstractpub_date
1993-08-01 00:00:00pages
2030-3issue
8eissn
0014-2980issn
1521-4141journal_volume
23pub_type
杂志文章abstract::The thymus provides the microenvironment in which thymocytes develop into mature T cells, and interactions with thymic stromal cells are thought to provide the necessary signals for thymocyte maturation. Recognition of self-MHC by T cells is a basic requirement for mature T-cell functions, and those thymocytes that do...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.201142338
更新日期:2012-07-01 00:00:00
abstract::Helicobacter pylori is a Gram-negative bacterium that causes a variety of gastrointestinal diseases, such as duodenal ulcer and gastric carcinoma. The T cell response against H. pylori is thought to contribute to the pathogenesis of these diseases. Here, we show that mouse-adapted H. pylori is able to polyclonally act...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636676
更新日期:2007-07-01 00:00:00
abstract::The death of individual cells is a frequent and physiological event in the mammalian immune system and most often occurs by apoptosis. It is becoming increasingly clear that cell death is also induced during bacterial infections. Here we report that, in addition to the apoptotic form already established, a necrosis-li...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324582
更新日期:2004-05-01 00:00:00
abstract::The molecular mimicry represented by cross-recognition of determinants shared by unrelated antigens by antibodies or T cells is of broad immunological interest. In this study, we analyzed the cross-recognition by CD4+ T cells of a peptide epitope shared by two mycobacterial proteins of diverse sequence, represented by...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830251128
更新日期:1995-11-01 00:00:00
abstract::The activating NK cell receptor NKG2D binds to numerous stress-induced cell surface glycoproteins with MHC class I-related ectodomains. In humans, NKG2D ligands (NKG2DL) are members of the MHC-encoded MIC and non-MHC-encoded ULBP families of proteins. The redundancy of NKG2DL raises questions about unique features ass...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200839074
更新日期:2009-06-01 00:00:00
abstract::A monoclonal antibody, B20.1, was generated by fusing spleen cells from a Lou rat immunized with a soluble alpha/beta T cell receptor (TcR; V alpha 2/V beta 2) to mouse myeloma cells. Analysis of a panel of V alpha 2 mRNA-expressing T cell lines, hybridomas and transfectants revealed that the B20.1 antibody was specif...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220217
更新日期:1992-02-01 00:00:00
abstract::Despite accounting for only a minor fraction of all cells in Hodgkin's lymphoma tissue, the Hodgkin and Reed-Sternberg (HRS) cells represent the malignant tumor cell clone in Hodgkin's disease (HD). By far the most abundant cell type in the tumor tissue are CD4+ T cells. Some of them intimately associate with HRS cell...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199808)28:08<2424::AID-IMM
更新日期:1998-08-01 00:00:00
abstract::Granzyme A (TSP-1) is a serine esterase expressed in cytotoxic T and natural killer (NK) cell lines. Its presence in in vivo primed T cells is disputed at present. Here we show that normal rat NK cells contain granzyme A in vivo and that its expression is augmented by their short-term in vitro treatment with IL 2. Gra...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190328
更新日期:1989-03-01 00:00:00
abstract::LIGHT is a recently identified member of the TNF superfamily that is up-regulated on activated T cells and can cooperate with CD40L to condition DC for the priming of CTL. In this report, we show that an intracellular pool of LIGHT is found selectively in some CD45RA-CD27+CD4+ "central" memory T cells and to a greater...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324410
更新日期:2003-11-01 00:00:00
abstract::Cells from the monoclonal B cell lymphoma I.29 expressing surface IgM (mu +) are capable of differentiating in vitro to IgM secretion and of switching to IgA or IgE production in response to lipopolysaccharide (LPS) stimulation. To determine whether a single mu + B cell is capable of undertaking both differentiative p...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830170419
更新日期:1987-04-01 00:00:00
abstract::CD40 ligand (CD40L) is a 33-kDa type II membrane glycoprotein induced on T cells upon activation. CD40L has previously been shown to induce proliferation of resting B cells, immunoglobulin (Ig) secretion from B cells cultured with cytokines and cytokine secretion and tumoricidal activity from monocytes. In this report...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230941
更新日期:1993-09-01 00:00:00
abstract::Differential screening of a subtracted cDNA library led to the detection of two distinct but homologous mouse cDNA, called CTLA-2 alpha and CTLA-2 beta. The corresponding transcripts have a tissue distribution restricted to T lymphocytes, where they are inducible upon activation, and to mast cells. The open-frame regi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190409
更新日期:1989-04-01 00:00:00
abstract::Activation of invariant natural killer T (iNKT) cells by treatment with their α-galactosyl ceramide ligand provides therapeutic benefits in several immune inflammatory settings. Given the artificial nature of this stimulation, the natural regulatory functions of iNKT remain uncertain. Addressing this issue in a mouse ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040647
更新日期:2011-02-01 00:00:00
abstract::Regulatory T (T(reg)) cells play a key role in the maintenance of the immune system homeostasis. T(reg) cells can be generated in the periphery under control of TGF-beta, a cytokine involved in the negative control of the immune system. However, TGF-beta cooperates with IL-6 in the generation of Th17 cells, a novel cl...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737766
更新日期:2007-11-01 00:00:00
abstract::The tight-skin (Tsk/+) mutant mouse, a putative murine model of scleroderma, is characterized primarily by the excessive deposition of collagen and other extracellular matrix molecules in the dermis, and also by a developmentally acquired defect in pulmonary architecture. Passive transfer experiments have suggested an...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199809)28:09<2619::AID-IMM
更新日期:1998-09-01 00:00:00
abstract::The immaturity of the neonatal immune system in mice is associated with defective IFN-gamma production and Th2-biased immune responses. In this study, infants vaccinated at birth with BCG produced similar concentrations of IFN-gamma in response to PPD and showed similar frequencies of IFN-gamma-producing lymphocytes a...
journal_title:European journal of immunology
pub_type: 临床试验,杂志文章
doi:10.1002/1521-4141(200105)31:5<1531::AID-IMMU1531>3
更新日期:2001-05-01 00:00:00
abstract::Studies based on either MHC class II-knockout or CD4+ T cell-depleted murine models have demonstrated a critical role for CD4+ T cells in the generation of CD8+ T cell memory. However, it is difficult to extend these findings to immunocompromised humans where a complete loss of CD4+ T cells is rarely observed. Here, w...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737933
更新日期:2008-07-01 00:00:00
abstract::Elucidating the signaling events that promote T-cell tolerance versus activation provides important insights for manipulating immunity in vivo. Previous studies have suggested that the absence of PKCtheta results in the induction of anergy and that the balance between the induction of the transcription factors NFAT, A...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200939445
更新日期:2010-03-01 00:00:00
abstract::Deoxyguanosine (dGuo) has been implicated as the toxic metabolite causing a severe impairment of cellular immunity in children with a genetic deficiency of purine nucleoside phosphorylase (PNP). In peripheral blood T cells of normal donors both the pathway which leads to phosphorylation of dGuo (ultimately resulting i...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160412
更新日期:1986-04-01 00:00:00
abstract::In this study we examined the effect of interleukin 4 (IL 4) on T cell activation and proliferation via the alternative CD2 pathway. To this end highly purified human resting T cells were cultured with a stimulating pair of anti-CD2 monoclonal antibodies in the absence of accessory signals from monocytes. Addition of ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200724
更新日期:1990-07-01 00:00:00
abstract::IL-23, a proximal regulator of IL-17, may be a major driving force in the induction of autoimmune inflammation. We have used a model of subdiabetogenic treatment with multiple low doses of streptozotocin (MLD-STZ; 4 x 40 mg/kg body weight) in male C57BL/6 mice to study the effect of IL-23 on immune-mediated beta cell ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200535325
更新日期:2006-01-01 00:00:00
abstract::Naive and memory CD4 T cells differ in cell surface phenotype, function, activation requirements, and modes of regulation. To investigate the molecular bases for the dichotomies between naive and memory CD4 T cells and to understand how the T cell receptor (TCR) directs diverse functional outcomes, we investigated pro...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270838
更新日期:1997-08-01 00:00:00
abstract::CD27, which belongs to the tumor necrosis factor receptor family, is expressed on germinal center (GC) but not on naive B cells, suggesting an important function of this molecule in the regulation of the GC reaction. We described here the expression of CD70, which is the ligand for CD27. We observed that in most tonsi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260508
更新日期:1996-05-01 00:00:00
abstract::We have studied the effect of T cell receptor (TcR) cross-linking and TcR-CD4 cross-linking on the induction of interleukin (IL) 2 and IL 2 receptor mRNA expression in peripheral T cells. We confirm our previous results using bioassays, suggesting that CD4 is involved in T cell activation by up-regulating the expressi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200731
更新日期:1990-07-01 00:00:00
abstract::The recognition of lipid antigens by T cells is a complex and fascinating phenomenon. The MHC-like molecules of the CD1 family have evolved to present a wide variety of both self and foreign lipids for recognition by T-cell receptors. While much progress has been made in our understanding of the NKT cells that recogni...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
doi:10.1002/eji.201141408
更新日期:2011-03-01 00:00:00
abstract::Traumatic brain injury (TBI) elicits innate inflammatory responses that can lead to secondary brain injury. To better understand the mechanisms involved in TBI-induced inflammation, we examined the nature of macrophages responding to TBI in mice. In this model, brain macrophages were increased >20-fold the day after i...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201243084
更新日期:2013-08-01 00:00:00
abstract::A recombinant Plasmodium vivax circumsporozoite (CS) protein (rPvCS-1) has been investigated as a possible malaria sporozoite vaccine candidate. Experiments were carried out to determine whether sporozoite-specific memory cells develop in Swiss Webster mice immunized with rPvCS-1. Challenge of rPvCS-1-immunized mice w...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830171213
更新日期:1987-12-01 00:00:00
abstract::In this study the genetic control of major histocompatibility complex (MHC) class II gene expression during the transition from B cell to plasma cell has been analyzed. Class II molecules are not expressed in plasma cells because of an active suppression resulting in the abrogation of class II gene transcription. We s...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830261028
更新日期:1996-10-01 00:00:00
abstract::Incorporation of an 11 amino acid region of the HIV TAT protein transduction domain (TAT PTD) into proteins facilitates rapid, efficient entry into cells. We previously showed that rTAT-PTD-OVA-transduced dendritic cells (DC) stimulated antigen (Ag)-specific CTL and Th cells, and vaccinated against OVA-expressing tumo...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200323709
更新日期:2003-04-01 00:00:00
abstract::Checkpoint blockade of CTLA-4 results in long-lasting survival benefits in metastatic cancer patients. However, patients treated with CTLA-4 blockade have suffered from immune-related adverse events, most likely due to the breadth of the induced T-cell activation. Here, we investigated the efficacy of a local low-dose...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201646583
更新日期:2017-02-01 00:00:00