Quantitative enzyme cytochemistry during human macrophage development.

Abstract:

:Integrating microdensitometry was used to study changes in the intracellular activity of 4 enzymes during macrophage development. Suspension cultures of blood monocytes from 19 healthy human subjects were examined at 0, 2, 4 and 6 d. Mononuclear phagocytes were harvested by glass adherence and standard methods were used for cytochemical staining for NADH dehydrogenase, succinate dehydrogenase, acid phosphatase and alpha-naphthyl butyrate esterase. All specimens from all subjects were stained at the same time and staining intensities in individual cells were measured at appropriate wavelengths. A highly significant increase in enzyme activity with culture time was found for all 4 enzymes. These increases in mitochondrial, lysosomal and ectoenzyme activities during development indicate the increasing functional capabilities of the macrophages.

journal_name

J Anat

journal_title

Journal of anatomy

authors

Sokol RJ,Hudson G,Wales JM,Goldstein DJ,James NT

subject

Has Abstract

pub_date

1993-08-01 00:00:00

pages

97-101

eissn

0021-8782

issn

1469-7580

journal_volume

183 ( Pt 1)

pub_type

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