Abstract:
PURPOSE:To evaluate the expression of lumican and decorin, the major proteoglycans of the adult corneal stroma, during the acquisition of corneal transparency in developing chick embryos. METHODS:mRNA levels of decorin and lumican were measured in total RNA extracted from corneas of days 9 to 18 of development by Northern blot analysis using a 32P-labeled cDNA clone to each proteoglycan. The synthesis lumican and decorin precursor proteins were determined by biosynthetically radiolabeling corneas from day 7 to 18 chick embryos with 35S-methionine, and then using antibodies specific for lumican and decorin core proteins to precipitate the radiolabeled precursor proteins. The accumulation of lumican and decorin was determined by fractionating extracts of day 7 to 18 embryonic corneas by DEAE chromatography into glycoprotein and proteoglycan fractions, and then analyzing each fraction by Western blot using antibodies to lumican and decorin. RESULTS:Lumican and decorin mRNA increased from day 9 to day 18, with respect to beta-actin. The rate of decorin precursor protein synthesis remained relatively low and constant throughout development, but lumican precursor protein synthesis increased dramatically between days 7 and 9 of embryonic development, to a value 80-fold higher than that of decorin, and then decreased exponentially through day 18. Lumican with polylactosamine (nonsulfated keratan sulfate) side chains was detected in extracts of corneas as early as day 7 of embryonic development, and continued to accumulate within the cornea through day 18. Decorin and lumican with sulfated glycosaminoglycan side chains (ie, proteoglycans), however, were not detected in corneal extracts until day 15, when transparency starts to increase, and then accumulated considerably within the cornea by day 18. CONCLUSIONS:The results of these studies suggest that decorin and lumican expression are independently regulated during the period of acquisition of corneal transparency. The switch in production of the polylactosamine form of lumican to the proteoglycan form of lumican at the onset of increasing corneal transparency suggests that the sulfation of lumican may be important for the development of corneal transparency.
journal_name
Invest Ophthalmol Vis Scijournal_title
Investigative ophthalmology & visual scienceauthors
Cornuet PK,Blochberger TC,Hassell JRsubject
Has Abstractpub_date
1994-03-01 00:00:00pages
870-7issue
3eissn
0146-0404issn
1552-5783journal_volume
35pub_type
杂志文章abstract:PURPOSE:To examine the relationship between circulating inflammatory markers, hemostatic factors, and age-related maculopathy (ARM). METHODS:A population-based, cross-sectional case-control study drawn from the Blue Mountains Eye Study included 159 early and 38 late ARM cases, and 433 controls matched for age, gender,...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.06-0223
更新日期:2007-05-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
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journal_title:Investigative ophthalmology & visual science
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更新日期:2017-12-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1998-11-01 00:00:00
abstract:PURPOSE:We determined the causes and five-year incidence of blindness and visual impairment (VI) in an adult, urban Chinese population. METHODS:Participants underwent a comprehensive eye examination at baseline in 2003 and then five years later. The World Health Organization (WHO) and United States (US) definitions we...
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更新日期:2013-06-14 00:00:00
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journal_title:Investigative ophthalmology & visual science
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更新日期:2015-09-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.03-0769
更新日期:2004-04-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1987-04-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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更新日期:2012-11-29 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.02-0162
更新日期:2003-01-01 00:00:00
abstract::N-formimidoyl thienamycin (MK-787) is a new beta-lactam with potent activity against both aerobic and anaerobic gram-positive and gram-negative bacteria. Its spectrum and activity suggest it may be useful in treatment of complicated intraocular infections. Its ocular penetration was studied in New Zealand white rabbit...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1983-08-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.15-17089
更新日期:2015-11-01 00:00:00
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pub_type: 杂志文章
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journal_title:Investigative ophthalmology & visual science
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更新日期:2013-10-09 00:00:00
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pub_type: 杂志文章
doi:10.1167/iovs.07-1543
更新日期:2008-08-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.08-3032
更新日期:2009-08-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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journal_title:Investigative ophthalmology & visual science
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journal_title:Investigative ophthalmology & visual science
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doi:
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更新日期:2020-10-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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doi:
更新日期:1985-01-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 临床试验,杂志文章,多中心研究
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更新日期:2014-11-04 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章,多中心研究
doi:10.1167/iovs.10-6987
更新日期:2011-06-21 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1993-11-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
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更新日期:2013-02-01 00:00:00
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更新日期:2009-06-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
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更新日期:2011-03-25 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.04-0772
更新日期:2005-01-01 00:00:00