Abstract:
:A major and a minor ascorbate free radical (AFR) reductase were separated from the soluble fraction in the human lens cortex by DEAE-cellulose ion-exchange column chromatography. These AFR reductases also exhibited diaphorase activity using dichlorophenolindophenol and ferricyanide as electron acceptors. The major AFR reductase was partially purified by 5'AMP-Sepharose 4B affinity column chromatography. This partially purified AFR reductase showed a single band of diaphorase activity in native polyacrylamide disc gel electrophoresis. This activity band corresponded to the major protein observed in protein staining by Coomassie Brilliant Blue. However, the protein staining by Coomassie Brilliant Blue showed this activity band surrounded by diffused staining. Molecular weight of the partially purified AFR reductase was determined to be 32 kDa by gel filtration, and the apparent Km value for AFR was about 15 microM. This major lens AFR reductase could be distinguished from soluble Neurospora, Euglena and cucumber AFR reductases, and from two ubiquitous enzymes with reduction activity of AFR and/or foreign compounds, ie, NADH-cytochrome b5 reductase and DT-diaphorase, by their molecular weights, Km values and/or ion-exchange chromatographic behaviors.
journal_name
Jpn J Ophthalmoljournal_title
Japanese journal of ophthalmologyauthors
Bando M,Obazawa Hsubject
Has Abstractpub_date
1994-01-01 00:00:00pages
1-9issue
1eissn
0021-5155issn
1613-2246journal_volume
38pub_type
杂志文章abstract:BACKGROUND:We present the pathological findings at orbital exenteration in a patient with Wyburn-Mason syndrome who underwent transarterial embolization. CASE:A 31-year-old man with a 10-year history of gradual exacerbation of left exophthalmos and left cheek swelling was found to have facial and orbital arteriovenous...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-008-0563-5
更新日期:2008-07-01 00:00:00
abstract::The effects of N-demethylated carbachol on intraocular pressure and pupil size were studied in 18 ocular hypertensive patients. Topical administration of N-demethylated carbachol in 9%, 15% and 30% solutions has been demonstrated to bring about miosis which was found to be dose-dependent. No significant change of intr...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1982-01-01 00:00:00
abstract:PURPOSE:The aim of this study was to investigate the usefulness of laser speckle flowgraphy (LSFG) for the differentiation of acute nonarteritic ischemic optic neuropathy (NAION) from anterior optic neuritis (ON). METHODS:To investigate blood flow in the optic disc under normal conditions, NAION, and anterior ON, we c...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-013-0246-8
更新日期:2013-07-01 00:00:00
abstract:PURPOSE:We have found that two aspartyl (Asp-151 and Asp-58) residues of alphaA-crystallin are inverted and isomerized to the biologically uncommon D-beta-Asp residues during aging. In order to elucidate the correlation between the formation of the D-beta-Asp isomer and the environment surrounding the Asp in the protei...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(00)00202-1
更新日期:2000-07-01 00:00:00
abstract:PURPOSE:To evaluate the effect of tetramethylpyrazine on the elevation of aqueous flare and intraocular pressure (IOP) induced by prostaglandin (PG) E(2) and PGE(2) receptor (EP) agonists. METHODS:PGE(2) or EP agonists (11-deoxy PGE(1), EP(2) agonist; 17-phenyl trinor PGE(2), EP(1) and EP(3) agonist; or sulprostone, E...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(01)00320-3
更新日期:2001-05-01 00:00:00
abstract:PURPOSE:To measure changes in choroidal thickness (CT), retinal thickness (RT), and axial length (AL) accompanying intraocular pressure (IOP) increase and to investigate the changes in axial eye dimensions induced by IOP increase. METHODS:Thirty-four eyes of 34 patients undergoing a diagnostic provocative test for pri...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-012-0173-0
更新日期:2012-11-01 00:00:00
abstract::Ca movements in mechanical responses of the isolated sphincter and dilator muscles of the rabbit iris to some drugs were studied. D600, a Ca-blocker, was practically ineffective in doses which relax the vascular and intestinal smooth muscles, while other smooth muscle relaxants were effective on the iris muscles in th...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1983-01-01 00:00:00
abstract::Cell population and proportion of each component cell type in preretinal membranes formed after retinal detachment were studied by light microscopy (LM) and transmission electron microscopy (TEM). The preretinal membranes were surgically obtained from 20 proliferative vitreoretinopathy (PVR) and 7 macular pucker patie...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1986-01-01 00:00:00
abstract:PURPOSE:To investigate the process of repair in the epithelial cells and the reconstruction in lens fibers of a mouse lens that has developed opacity because of a large-scale perforating injury. METHODS:Lenses of 4-week-old mice were perforated with a needle through the cornea to induce the development of traumatic ca...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(03)00075-3
更新日期:2003-07-01 00:00:00
abstract::We explored the involvement of CD44 isoforms in lymphoproliferative disorder (LPD) of the lacrimal gland of a Sjögren's syndrome patient with unilateral LPD. The CD44 variant with the v6 exon was selectively detected from infiltrating lymphocytes in the gland with LPD, but not from infiltrating lymphocytes in the norm...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(97)00072-5
更新日期:1997-09-01 00:00:00
abstract:PURPOSE:Sweet's syndrome (SS) is a skin disorder clinically characterized by fever, neutrophilia, and painful edematous plaques that occasionally causes posterior uveitis. We present two cases of SS with panuveitis resembling Behçet's disease (BD). SUBJECTS:Two patients with panuveitis associated with SS. OBSERVATION...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-012-0129-4
更新日期:2012-05-01 00:00:00
abstract:BACKGROUND:Full-thickness macular holes usually develop in the elderly population. To the best of our knowledge, there has been no written report of a nontraumatic macular hole in a pediatric patient. CASE:A 10-year-old girl noticed decreased central vision in her left eye without any history of trauma. OBSERVATIONS:...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-005-0231-y
更新日期:2005-11-01 00:00:00
abstract:PURPOSE:To assess intravitreal bevacizumab (IVB) for choroidal neovascularization (CNV) secondary to age-related macular degeneration (AMD) in Japanese patients. METHODS:Forty-seven patients treated with IVB for newly diagnosed subfoveal or juxtafoveal CNV (predominantly classic CNV, 15 eyes; minimally classic CNV, 11...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-009-0775-3
更新日期:2010-03-01 00:00:00
abstract:PURPOSE:To investigate cytokine mRNA expression during the inflammatory process induced in the contralateral eyes by uniocular inoculation of herpes simplex virus type 1 (HSV-1) via the anterior chamber. METHODS:BALB/c mice were inoculated in the anterior chamber with 5 x 10(4) plaque-forming units of HSV-1 (KOS). mRN...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(02)00700-1
更新日期:2003-03-01 00:00:00
abstract::To investigate the true structural changes in retinal cotton wool spots, serial sections of several blocks of retinal corresponding to cotton wool spots obtained from two hypertensive cases were studied by light and electron microscopy. Occlusion of the feeder arteriole and capillaries, and numerous vacuoles of variou...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1983-01-01 00:00:00
abstract::Fibronectin (FN) has been detected at the site of corneal wounds. To investigate whether FN is supplied by the cornea itself or by extracorneal sources, we cultured small blocks of rabbit cornea in vitro with and without autologous serum (5%). The sliding rate of epithelial cells was determined and localization of FN ...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1982-01-01 00:00:00
abstract:PURPOSE:To elucidate the current clinical practice patterns of branch retinal vein occlusion (BRVO) management by retina specialists in Japan in the era of anti-vascular endothelial growth factor (VEGF) therapy. STUDY DESIGN:A voting survey using an answer pad system. METHODS:On May 28, 2017, forty-one retina special...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章,多中心研究
doi:10.1007/s10384-019-00685-4
更新日期:2019-09-01 00:00:00
abstract:PURPOSE:To compare endothelial damage of the donor cornea in relation to the method of inserting the donor cornea into the recipient anterior chamber during Descemet-stripping automated endothelial keratoplasty (DSAEK). METHODS:Fifteen enucleated pig eyes were used. The donor corneoscleral buttons were mounted in an a...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-009-0713-4
更新日期:2009-09-01 00:00:00
abstract::Three cases of medullated nerve fibers of the retina were seen. They had medullated nerve fibers in both the upper and lower quadrants of the temporal retina, whereby the temporal raphe of the retinal nerve fiber layer could be visualized. Some nerve fibers from the upper and lower quadrants crossed each other on the ...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
abstract::Takayasu disease was first reported in 1908 by Mikito Takayasu as "a case of peculiar changes in the central retinal vessels." Because in these patients the pulse of the radial artery is impalpable, investigations focusing on the ischemic symptoms of the upper body were conducted. In 1948, Shimizu and Sano named this ...
journal_title:Japanese journal of ophthalmology
pub_type: 传,历史文章,杂志文章
doi:10.1007/s10384-009-0650-2
更新日期:2009-03-01 00:00:00
abstract::The spectral sensitivity of the ERG c-wave was studied in the chicken under yellow or red light adaptation of various intensities, ie, 1, 3, 4 and 5 W/m2 at the corneal surface in the former, and 3, 4 and 5 W/m2 in the latter. The peak wavelength of the spectral sensitivity curve of the c-wave amplitude under both yel...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract:PURPOSE:To evaluate the effects of topical latanoprost and timolol in combined therapy on retinal blood flow and tissue circulation in the optic nerve head (ONH) of the cynomolgus monkey. METHODS:Latanoprost (30 microL, 0.005%) was instilled once daily and timolol (30 microL, 0.5%) twice daily for 6 days into 1 eye, a...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(99)00217-8
更新日期:2000-05-01 00:00:00
abstract:PURPOSE:To evaluate cases with a retinal pigment epithelium (RPE) aperture using polarization-sensitive optical coherence tomography (PS-OCT). STUDY DESIGN:Retrospective consecutive case series. METHODS:A retrospective study that included three eyes (three patients) with RPE aperture and age-related macular degenerat...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-020-00787-4
更新日期:2020-11-10 00:00:00
abstract::Conventional vitreoretinal surgery for proliferative vitreoretinopathy is unsatisfactory despite numerous innovations over the past 20 years. Silicone oil injections in this series resulted in 70% reattachment of these end-stage cases, but within twelve months over 90% had no useful visual result. The development of a...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
abstract:PURPOSE:To evaluate the efficacy and safety of intravitreal drug injections using a short 34-gauge needle. STUDY DESIGN:Retrospective study. METHODS:This study included patients with age-related macular degeneration, diabetic macular edema, or macular edema associated with retinal vein occlusion. We reviewed the medi...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-019-00663-w
更新日期:2019-05-01 00:00:00
abstract::The clinical characteristics of 20 eyes of 20 consecutive patients with traumatic macular hole were studied retrospectively. The macular holes were elliptical with irregular edges in 19 eyes (95%) and ranged in size from 0.2 to 0.5 DD (disc diameter). Posterior vitreous detachment (PVD) was found in only 3 eyes (15%);...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract:PURPOSE:To determine whether exposure to ozonated solution alters the morphology of corneal endothelial cells in rats and to examine the protective effect of ascorbic acid. METHODS:The anterior chambers of rat eyes were filled with 4 ppm of ozonated solution. Some were left in that state, while others were flushed out...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-008-0629-4
更新日期:2009-03-01 00:00:00
abstract:BACKGROUND:We present 6 cases of bacterial infection that developed after porous orbital implant surgery. CASES:Five patients with hydroxyapatite implants showed lid swelling, discharge, and suppurative granuloma 14 days to 3 years after surgery. The hydroxyapatite implants were removed 14 days to 41 months postoperat...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(03)00137-0
更新日期:2003-09-01 00:00:00
abstract:PURPOSE:To determine whether photoreceptor degeneration in transgenic (Tg) rabbits carrying the Pro347Leu rhodopsin mutation alters the neural activity of the middle and inner retinal neurons. METHODS:Multifocal electroretinograms (mfERGs) were recorded from eight 12-week-old Tg rabbits both before and after intravitr...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-010-0842-9
更新日期:2010-09-01 00:00:00
abstract:PURPOSE:To describe the characteristics and clinical course of patients with active posterior syphilitic uveitis evaluated between 1991 and 2009. METHODS:Retrospective chart review. RESULTS:Thirteen patients with active posterior syphilitic uveitis were identified at a single institution. All were men, and all with a...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-011-0053-z
更新日期:2011-09-01 00:00:00