Abstract：
:When Leishmania donovani promastigote forms, were cultured in TC-199 medium at 28 degrees C and subsequently incubated at 38 degrees C, they turned into aflagellate (amastigote-like) forms. A return of the incubation-culture temperature to 28 degrees C these amastigote-like forms to revert to promastigotes. The amastigotes obtained by heat-shock, were viable and retained antigenic capacity being recognized by the sera of naturally infected patients. These forms, remained also capable of multiplying inside the J-774A.1 macrophages. When the amastigote-like forms are kept in culture at 38 degrees C retained their rounded appearance and their biological characteristics for more than 3 months subculturing every 6 days. These amastigote-like forms, when used for subcultures at 28 degrees C, transformed into promastigotes capable of multiplying as flagellate forms. The amastigote-like forms obtained in vitro can be used in biochemical studies related to chemotherapy and immunology studies, as part of an effort to combat this parasite. The end-products of of glycolysis were studied in both the amastigote-like and promastigote forms of L. donovani, by proton magnetic resonance analysis of the culture media. Alanine, succinate, and acetate, were predominant, and to a lesser extent pyruvate, glycine and D-lactate. Our results suggest that both forms of Leishmania use different biochemical strategies to obtain their energy.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Castilla JJ,Sanchez-Moreno M,Mesa C,Osuna Adoi
10.1007/BF00928929subject
Has Abstractpub_date
1995-01-26 00:00:00pages
89-97issue
2eissn
0300-8177issn
1573-4919journal_volume
142pub_type
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