Abstract:
:p21Sdi1 (also known as Cip1 and Waf1), an inhibitor of DNA synthesis cloned from senescent human fibroblasts, is an inhibitor of G1 cyclin-dependent kinases (Cdks) in vitro and is transcriptionally regulated by wild-type p53. In addition, p21Sdi1 has been found to inhibit DNA replication by direct interaction with proliferating cell nuclear antigen. In this study we analyzed normal human fibroblast cells arrested in G0 and determined that an excess of p21Sdi1 was present after immunodepletion of various cyclins and Cdks, in contrast to mitogen-stimulated cells in early S phase. Expression of antisense p21Sdi1 RNA in G0-arrested cells resulted in induction of DNA synthesis as well as entry into mitosis. These results suggest that p21Sdi1 functions in G0 and early G1 and that decreased expression of the gene is necessary for cell cycle progression.
journal_name
Proc Natl Acad Sci U S Aauthors
Nakanishi M,Adami GR,Robetorye RS,Noda A,Venable SF,Dimitrov D,Pereira-Smith OM,Smith JRdoi
10.1073/pnas.92.10.4352subject
Has Abstractpub_date
1995-05-09 00:00:00pages
4352-6issue
10eissn
0027-8424issn
1091-6490journal_volume
92pub_type
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