Abstract：
:Use of the polymerase chain reaction (PCR) to amplify variable numbers of tandem repeat (VNTR) loci has become widely used in genetic typing. Unfortunately, preferential amplification of small allelic products relative to large allelic products may result in incorrect or ambiguous typing in a heterozygous sample. The mechanism for preferential amplification has not been elucidated. Recently, PNA oligomers (peptide nucleic acids) have been used to detect single base mutations through PCR clamping. PNA is a DNA mimic that exhibits several unique hybridization characteristics. In this report we present a new application of PNA which exploits its unique properties to provide enhanced amplification. Rather than clamping the PCR, PNA is used to block the template making it unavailable for interstrand and intrastrand interactions while allowing polymerase to displace the PNA molecules and extend the primer to completion. Preferential amplification is reduced and overall efficiency is enhanced.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Demers DB,Curry ET,Egholm M,Sozer ACdoi
10.1093/nar/23.15.3050subject
Has Abstractpub_date
1995-08-11 00:00:00pages
3050-5issue
15eissn
0305-1048issn
1362-4962pii
5j0011journal_volume
23pub_type
杂志文章abstract：:The HSV gene encoding ICP4 is negatively regulated and the HSV gene encoding thymidine kinase is positively regulated by ICP4 in vivo. We report that ICP4 is a component of a stable complex that contains protein and a sequence of approximately 28 nucleotides that span the ICP4 gene transcription initiation site. The a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.2.555
更新日期：1988-01-25 00:00:00
abstract：:The laminin (LM)-database, hosted at http://www.lm.lncc.br, was published in the NAR database 2011 edition. It was the first database that provided comprehensive information concerning a non-collagenous family of extracellular matrix proteins, the LMs. In its first version, this database contained a large amount of in...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt901
更新日期：2014-01-01 00:00:00
abstract：:Digital PCR provides high sensitivity and unprecedented accuracy in DNA quantification, but current approaches require dedicated instrumentation and have limited opportunities for multiplexing. Here, we present an isothermal platform for digital enumeration of DNA reaction products in multiplex via standard fluorescen...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa419
更新日期：2020-07-27 00:00:00
abstract：:The evolution of regulatory networks in Bacteria has largely been explained at macroevolutionary scales through lateral gene transfer and gene duplication. Transcription factors (TF) have been found to be less conserved across species than their target genes (TG). This would be expected if TFs accumulate mutations fas...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa162
更新日期：2020-05-07 00:00:00
abstract：:DNA primase has been partially purified from wheat germ. This enzyme, like DNA primases characterized from many procaryotic and eucaryotic sources, catalyses the synthesis of primers involved in DNA replication. However, the wheat enzyme differs from animal DNA primase in that it is found partially associated with a D...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.16.4867
更新日期：1990-08-25 00:00:00
abstract：:Publicly available database of co-expressed gene sets would be a valuable tool for a wide variety of experimental designs, including targeting of genes for functional identification or for regulatory investigation. Here, we report the construction of an Arabidopsis thaliana trans-factor and cis-element prediction data...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl783
更新日期：2007-01-01 00:00:00
abstract：:Contrary to the expectation, the Merrifield polystyrene resin, 2% cross-linked by divinylbenzene, is as efficient as the polyacrylmorpholide resin for the synthesis of polydeoxyribonucleotides using a phosphotriester method. On the Merrifield resin, the tetradecamer, dTpCpGpTpCpApApCpTpGpGpCpTpT, and the hexadecamer, ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/8.22.5507
更新日期：1980-11-25 00:00:00
abstract：:Topoisomerases (topos) maintain DNA topology and influence DNA transaction processes by catalysing relaxation, supercoiling and decatenation reactions. In the cellular milieu, division of labour between different topos ensures topological homeostasis and control of central processes. In Escherichia coli, DNA gyrase is...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks843
更新日期：2012-11-01 00:00:00
abstract：:The 'sandwich' binding format, which uses two reagents that can bind simultaneously to a given analyte, is the gold standard in diagnostics and many biochemical techniques. One of the bottlenecks in creating a sandwich assay is identifying pairs of reagents that bind non-competitively to the target. To bridge this gap...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx688
更新日期：2017-10-13 00:00:00
abstract：:Starting from a synthetic E. coli promoter with the consensus sequences at -35 and -10 regions, a sequence CAT frequently occurred in the RNA start points of natural promoters was introduced in the downstream of the consensus sequences, and the sequences around the RNA start points as well as the relative positions of...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/13.11.4085
更新日期：1985-06-11 00:00:00
abstract：:We have isolated a second gene (MLS1), which in addition to DAL7, encodes malate synthase from S. cerevisiae. Expression of the two genes is specific for their physiological roles in carbon and nitrogen metabolism. Expression of MLS1, which participates in the utilization of non-fermentable carbon sources, is sensitiv...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.21.5677
更新日期：1992-11-11 00:00:00
abstract：:Fully protected diastereoisomers of deoxyguanylyl (3' leads to 5') deoxyadenosine stereospecifically labelled on phosphorus with oxygen-18 have been synthesized by oxidation of phosphite triester intermediates in the presence of 18O-labelled water. The diastereoisomers have been chromatographically separated and their...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/11.20.7087
更新日期：1983-10-25 00:00:00
abstract：:We present MultiGO, a web-enabled tool for the identification of biologically relevant gene sets from hierarchically clustered gene expression trees (http://ekhidna.biocenter.helsinki.fi/poxo/multigo). High-throughput gene expression measuring techniques, such as microarrays, are nowadays often used to monitor the exp...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl694
更新日期：2006-01-01 00:00:00
abstract：:Structural domains are considered as the basic units of protein folding, evolution, function and design. Automatic decomposition of protein structures into structural domains, though after many years of investigation, remains a challenging and unsolved problem. Manual inspection still plays a key role in domain decomp...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg189
更新日期：2003-02-01 00:00:00
abstract：:Cellular membranes feature dynamic submicrometer-scale lateral domains termed lipid rafts, membrane rafts or glycosphingolipid-enriched microdomains (GEM). Numerous proteomics studies have been conducted on the lipid raft proteome, however, interpretation of individual studies is limited by potential undefined contami...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky948
更新日期：2019-01-08 00:00:00
abstract：:The chromatographic profiles of isoaccepting tRNAs were analyzed at five time points during the 96 hr, dimethylsulfoxide induced, erythroid-like differentiation of Friend leukemia cells. Sixty-four isoaccepting species of tRNA for 16 amino acids were resolved by RPC-5 chromatography. The relative amounts of tRNAphe, t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/8.15.3467
更新日期：1980-08-11 00:00:00
abstract：:Differential scanning microcalorimetry (DSC), UV absorption and circular dichroism (CD) have been used to study structure and stability of linear (lin), open circular (oc), supercoiled (cd) and relaxed circular duplex (rd) DNA and calf thymus (CT) DNA. Investigations were made in low salt buffer and in the presence of...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.24.11737
更新日期：1988-12-23 00:00:00
abstract：:1,10-Phenanthroline-platinum(II)-ethylenediamine ( PEPt ) forms a crystalline complex with cytidine-3'-phosphate (3'-CMP) and its structure has been determined by X-ray crystallography. 3'-CMP molecules are hemiprotonated and form hydrogen-bonded pairs that stack above and below the phenanthroline-platinum(II) drug mo...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.8.3649
更新日期：1984-04-25 00:00:00
abstract：:Bacterial retrons consist of a reverse transcriptase (RT) and a contiguous non-coding RNA (ncRNA) gene. One third of annotated retrons carry additional open reading frames (ORFs), the contribution and significance of which in retron biology remains to be determined. In this study we developed a computational pipeline ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa1149
更新日期：2020-12-16 00:00:00
abstract：:The protein property prediction and testing database (PPT-DB) is a database housing nearly 30 carefully curated databases, each of which contains commonly predicted protein property information. These properties include both structural (i.e. secondary structure, contact order, disulfide pairing) and dynamic (i.e. orde...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm800
更新日期：2008-01-01 00:00:00
abstract：:The database of 3D interacting domains (3did, available online for browsing and bulk download at http://3did.irbbarcelona.org) is a catalog of protein-protein interactions for which a high-resolution 3D structure is known. 3did collects and classifies all structural templates of domain-domain interactions in the Prote...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt887
更新日期：2014-01-01 00:00:00
abstract：:The Rhizobium trifolii nod genes required for host-specific nodulation of clovers are located on 14 kb of Sym (symbiotic) plasmid DNA. Analysis of the nucleotide sequence of a 3.7 kb portion of this region has revealed open reading frames corresponding to the nodABCDEF genes. A DNA sequencing technique, using primer e...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.7.2891
更新日期：1986-04-11 00:00:00
abstract：:There are a few algorithms designed to solve the problem of the optimal alignment of one sequence, the pattern, of length m, with another, longer sequence the text, of length n. These algorithms allow mismatches, deletions and insertions. Algorithms to date run in O(mn) time. Let us define an integer, k, which is the ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.1.31
更新日期：1986-01-10 00:00:00
abstract：:In plant organelles, RNA editing alters specific cytidine residues to uridine in transcripts. All of the site-specificity factors of RNA editing identified so far are pentatricopeptide repeat (PPR) proteins. A defect in a specific PPR protein often impairs RNA editing at multiple sites, at which the cis-acting element...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks164
更新日期：2012-06-01 00:00:00
abstract：:Two dimensional PEI-cellulose thin layer chromatography can resolve sequentially degraded oligonucleotide fragments of tRNA. This technique entails the sequential degradation of the oligonucleotide with snake venom phosphodiesterase in the presence of bacterial alkaline phosphatase, and periodate oxidation followed by...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/4.10.3563
更新日期：1977-10-01 00:00:00
abstract：:Defects in the posttranscriptional modifications of mitochondrial tRNAs have been linked to human diseases, but their pathophysiology remains elusive. In this report, we investigated the molecular mechanism underlying a deafness-associated tRNAIle 4295A>G mutation affecting a highly conserved adenosine at position 37,...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa1225
更新日期：2021-01-25 00:00:00
abstract：:A restriction endonuclease, ApaI, has been partially purified from Acetobacter pasteurianus. This enzyme cleaves bacteriophage lambda DNA and Simian virus 40 DNA at one site, adenovirus-2 DNA at more than nine sites, but it does not cleave phi X174 DNA nor plasmid pBR322 DNA. This enzyme recognizes the sequence (formu...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/11.13.4409
更新日期：1983-07-11 00:00:00
abstract：:In the last decade polycistronic vectors have become essential tools for both basic science and gene therapy applications. In order to co-express heterologous polypeptides, different systems have been developed from Internal Ribosome Entry Site (IRES) based vectors to the use of the 2A peptide. Unfortunately, these me...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp716
更新日期：2009-11-01 00:00:00
abstract：:Tetrahymena thermophila mitochondrial DNA is a linear molecule with two tRNAs, large subunit beta (LSU beta) rRNA (21S rRNA) and LSU alpha rRNA (5.8S-like RNA) encoded near each terminus. The DNA sequence of approximately 550 bp of this region was determined in six species of Tetrahymena. In three species the LSU beta...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.1.327
更新日期：1988-01-11 00:00:00
abstract：:The National Center for Biotechnology Information (NCBI) provides a large suite of online resources for biological information and data, including the GenBank® nucleic acid sequence database and the PubMed® database of citations and abstracts published in life science journals. The Entrez system provides search and re...
journal_title：Nucleic acids research
pub_type： 杂志文章,评审
doi：10.1093/nar/gkaa892
更新日期：2021-01-08 00:00:00
