Abstract:
AIMS:To evaluate the effect of microwave irradiation on the staining quality of a range of commonly used primary antibodies in archival, formalin fixed, paraffin wax embedded material, with emphasis on antibodies that have previously worked successfully only on frozen tissue. METHODS:Immunocytochemistry (streptavidin-biotin complex technique) was performed on histological sections of a range of normal and pathological tissues, after varying treatment with microwave irradiation. The staining quality of each antibody was compared with that achieved without prior treatment of the sections or after enzyme predigestion. RESULTS:Microwave irradiation permitted successful immunostaining with 20 antibodies that stained only frozen tissues before. The staining characteristics of 21 antibodies that were already known to stain formalin fixed, paraffin wax embedded material were improved. Another 39 antibodies did not show enhanced staining with microwave irradiation. The method preserves tissue morphology and produces more consistent staining than that achieved by enzyme predigestion with many antibodies. Microwave irradiation may also allow some primary antibodies to be used at higher working dilutions. The citrate buffer used in this study avoids the necessity of exposure to heavy metal salts. CONCLUSIONS:Microwave antigen retrieval represents an important technical advance within immunocytochemistry that will greatly increase the range of antibodies which can be used to study formalin fixed, paraffin wax embedded tissues.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Cuevas EC,Bateman AC,Wilkins BS,Johnson PA,Williams JH,Lee AH,Jones DB,Wright DHdoi
10.1136/jcp.47.5.448subject
Has Abstractpub_date
1994-05-01 00:00:00pages
448-52issue
5eissn
0021-9746issn
1472-4146journal_volume
47pub_type
杂志文章abstract:AIMS:To study the homozygous deletion and methylation status of the 5' CpG island of the p16 and p15 genes (9p21) in a set of primary advanced head and neck squamous cell carcinomas (SCC) and to test whether inactivation of these genes by these mechanisms contributes to head and neck SCC development. METHODS:DNA was e...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.50.6.509
更新日期:1997-06-01 00:00:00
abstract::Two examples of intraplacental choriocarcinoma are described. Both were small and had arisen in otherwise normal third trimester placentas. The covering mantle of many of the villi adjacent to the choriocarcinomas was formed, either focally or wholly, of neoplastic trophoblastic tissue: it is only at this stage of the...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.41.10.1085
更新日期:1988-10-01 00:00:00
abstract::Cytomegalovirus (CMV) is a recognised cause of morbidity and mortality in immunocompromised individuals. This review will concentrate on recent advances in the understanding of the complex interplay between the host and parasite and the pathological consequences of perturbation of the host immune system. The classic v...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.54.2.84
更新日期:2001-02-01 00:00:00
abstract::One hundred cases of transitional cell carcinoma of the bladder were studied to determine whether squamous metaplasia and other histological features within the bladder can be of value in predicting outcome of treatment with radiotherapy. Sixty cases showed the changes of squamous metaplasia, and of this group 46 (78%...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.42.3.250
更新日期:1989-03-01 00:00:00
abstract:AIMS:(1) To investigate the immunohistochemical expression of the multidrug resistance gene (MDR1) product P-glycoprotein in histological samples from 31 hepatocellular carcinomas (HCCs); and (2) to correlate the results with cell proliferation, p53 expression, the disease-free interval, and cumulative patient survival...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.49.6.470
更新日期:1996-06-01 00:00:00
abstract::The Kallestad Corporation recently suggested that their new buffer system for the nephelometric detection of rheumatoid factor conferred advantages over existing systems. Two rate nephelometric procedures, the Kallestad QM-300 and the Beckman ICS-II (RF), were therefore compared. Sera (n = 157) were selected on the ba...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.43.3.243
更新日期:1990-03-01 00:00:00
abstract::The aggregating effects of adenosine diphosphate, thrombin, 5-hydroxytryptamine, tryptamine, adrenaline and noradrenaline, and tri-ethyl tin have been carefully compared. The first three compounds in some circumstances produce remarkably similar effects although there are important differences. The kinetics of aggrega...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.17.3.275
更新日期:1964-05-01 00:00:00
abstract:AIMS:Use of the polymerase chain reaction for the detection of Mycobacterium tuberculosis (TB PCR) as a basis for making clinical decisions on the initiation of antituberculosis treatment was studied. METHODS:A retrospective study involving a cohort of 155 patients being investigated for tuberculosis in an infectious ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2003.012658
更新日期:2004-03-01 00:00:00
abstract::A 72 year old woman presented complaining of nasal obstruction, rhinorrea, and epistaxis. At examination, a polypoid mass on the right posterior choana was revealed and subsequently removed. Light microscopic findings consisted of a diffuse proliferation of spindle shaped, pleomorphic cells with eosinophilic cytoplasm...
journal_title:Journal of clinical pathology
pub_type: 信件
doi:10.1136/jcp.55.11.872
更新日期:2002-11-01 00:00:00
abstract:BACKGROUND:A previous survey has highlighted the fact that most individuals in chemical pathology identifiable from specialist society membership failed to publish material in Medline cited journals during a five year period. It could be considered that published research that is not cited in other work is not useful u...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2003.012351
更新日期:2004-04-01 00:00:00
abstract::Pre-eclampsia complicated by deep jaundice occurred in a previously healthy primigravida. The main aetiological factor was disseminated intravascular coagulation; this caused both haemolysis and liver cell necrosis. Liver biopsy showed fibrin thrombi in the capillaries with microhaemorrhages and loss of periportal liv...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.30.3.212
更新日期:1977-03-01 00:00:00
abstract:AIMS:To determine the possible histogenesis of the intracranial variant of olfactory neuroblastoma. METHODS:Four specimens from three cases of intracranial olfactory neuroblastoma were studied by light microscopy and immuno-histochemistry, and electron microscopy in two cases. RESULTS:Light microscopical examination ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.45.4.299
更新日期:1992-04-01 00:00:00
abstract::A new sensitive nitrite test, the SRN test, was evaluated for its suitability as a reliable screening technique for the detection of bacteriuria. The SRN test was compared to a currently available nitrite test, the Microstix nitrite test, and the results obtained with both nitrite tests were assessed in comparison wit...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.34.7.723
更新日期:1981-07-01 00:00:00
abstract:AIMS:To investigate the correlation between centrally assessed human epidermal growth factor receptor 2 (HER2) immunohistochemistry (IHC) and fluorescence in situ hybridisation (FISH) results and response to treatment of patients with metastatic breast cancer enrolled in a first-line, phase II, open-label, 3-weekly tra...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2006.043562
更新日期:2008-01-01 00:00:00
abstract::A family with hypobetalipoproteinaemia was studied to examine the Lp(a) lipoprotein, lipoprotein cholesterol, and triglyceride composition of the serum lipids. Lp(a) lipoprotein was measured by immunoassay. Serum lipoproteins were separated by ultracentrifugation. Cholesterol and triglycerides were measured using stan...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.48.6.587
更新日期:1995-06-01 00:00:00
abstract::Normal haemoglobin is a tetramer molecule, consisting of two α and β haemoglobin chains. Haemoglobinopathies occur when abnormalities in these proteins are present. More than 1000 naturally occurring human haemoglobin variants with single amino acid substitution throughout the molecule have been identified and can be ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jclinpath-2018-205616
更新日期:2019-07-01 00:00:00
abstract::The results of measurement of gamma globulin in the cerebrospinal fluid by electrophoresis and by an immunological technique are compared. In some fluids marked discrepancies were found. Such discrepancies, when substantiated and better understood, may have diagnostic significance. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.16.4.370
更新日期:1963-07-01 00:00:00
abstract::Twenty five specimens of branchial cyst from the same number of patients have been examined. On staining with haematoxylin and eosin a consistent finding was that the mural lymphoid follicles were always aligned with their mantle zones towards the luminal epithelium. With conventional staining lymphatic sinuses were n...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.38.7.784
更新日期:1985-07-01 00:00:00
abstract::One hundred catalase-positive, coagulase-negative, Gram-positive cocci isolated in significant numbers from the urine of patients with urinary tract infections, provisionally subdivided by their sensitivity to nonoviocin, were classified according to a slightly model version of Baird-Parker's schemes (1965 and 1972). ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.28.4.270
更新日期:1975-04-01 00:00:00
abstract::Antibiotic discs from Oxoid, Mast, AB Biodisk, Difco, and Baltimore Biological Laboratories were compared, where discs of similar antibiotic content were available, in diffusion-sensitivity tests against organisms of known sensitivity. Discs from Oxoid and Mast gave zones 1-5 mm larger than discs from other manufactur...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.28.10.779
更新日期:1975-10-01 00:00:00
abstract::Lymphoid tissue of the endometrium was analysed by histological, immunohistological, and electron microscopical methods in 10 healthy uteri. A panel of monoclonal antibodies recognising macrophages (OKMI), HLA-DR antigen, B lymphocytes, T lymphocytes and their subsets, and dendritic reticulum cells was used in a two s...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.38.6.644
更新日期:1985-06-01 00:00:00
abstract::The relationship between the intensity of the delayed hypersensitivity reaction and the dose of antigen used for its elicitation change with time after sensitization. At three weeks the reactions to 100 mug of egg albumen were little different from those to 1 mug but at 14 weeks 100 mug gave much stronger reactions. T...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.26.9.687
更新日期:1973-09-01 00:00:00
abstract::Clinical trials have shown that many patients with breast cancer with limited sentinel lymph node (SLN) metastatic disease can safely avoid axillary lymph node dissection. Ultra-staging of initially negative SLNs may not confer additional clinical benefit. Despite this, protocols of 'enhanced pathological examination'...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2020-206629
更新日期:2020-10-22 00:00:00
abstract::Aztreonam blood agar, a new selective medium for Gram positive aerobic bacteria, was evaluated in comparison with conventional media for skin swabs. Aztreonam agar increased the number of isolates of Staphylococcus aureus by 17%. By producing purer growths on primary isolation, it significantly speeded up the identifi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.46.8.769
更新日期:1993-08-01 00:00:00
abstract::Angiogenesis plays a crucial role in melanoma metastasis and progression. In recent years, numerous studies have investigated the prognostic and clinical significance of this phenomenon, and the development of molecular techniques has enabled us to achieve a better understanding of angiogenesis in melanoma. Herein, we...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jclinpath-2015-203482
更新日期:2016-06-01 00:00:00
abstract:AIMS:The aim of this study is to explore DNA hypermethylation analysis in sputum and exhaled breath analysis for their complementary, non-invasive diagnostic capacity in lung cancer. METHODS:Sputum samples and exhaled breath were prospectively collected from 20 lung cancer patients and 31 COPD controls (Set 1). An add...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2014-202414
更新日期:2014-08-01 00:00:00
abstract::The pathological diagnoses and classification schemes for thyroid carcinoma have changed over the past 20 years and continue to do so. New entities have been described and molecular analyses have suggested better characterisation and grouping of certain tumours. Because some of the lesions have been named differently ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jclinpath-2013-201626
更新日期:2013-09-01 00:00:00
abstract::As part of a quality assurance programme at the Aga Khan University Medical Centre, Karachi, Pakistan, all hysterectomy specimens were reviewed from January 1986 to December 1989. Adenomyosis was found in 237 of the 419 (56.5%) specimens studied. Of these 237 patients, 232 (97.9%) were parous and 196 (82.8%) were in t...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.43.10.817
更新日期:1990-10-01 00:00:00
abstract:AIMS:To identify molecular characteristics to hepatitis C virus (HCV)-associated diffuse large B-cell lymphoma (DLBCL) through a comprehensive miRNAs expression profiling. METHODS:In this study, miRNA profiles were obtained from 37 patients with DLBCLs and 60 patients with reactive lymph nodes, equally distributed acc...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2014-202352
更新日期:2014-08-01 00:00:00
abstract:BACKGROUND:Fibrinogen plays a crucial role in the pathophysiology of tumour cell growth, invasion and metastasis. The aim of this study was to evaluate the prognostic significance of pretreatment plasma fibrinogen levels in patients with diffuse large B cell lymphoma (DLBCL) METHODS: Data from 372 patients with DLBCL, ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,多中心研究
doi:10.1136/jclinpath-2015-203356
更新日期:2016-04-01 00:00:00