Abstract:
AIMS:To study the homozygous deletion and methylation status of the 5' CpG island of the p16 and p15 genes (9p21) in a set of primary advanced head and neck squamous cell carcinomas (SCC) and to test whether inactivation of these genes by these mechanisms contributes to head and neck SCC development. METHODS:DNA was extracted from fresh tumours. Homozygous deletion was determined by the polymerase chain reaction (PCR) followed by hybridisation with the corresponding probe, radioactively labelled by the random priming method. Methylation status of the CpG island of the 5' region of these genes was assessed by digestion with the appropriate restriction enzymes followed by PCR and subsequent hybridisation with the corresponding probe. The presence of point mutations was determined by PCR-SSCP (single strand conformation polymorphism). RESULTS:The p16 and p15 genes were homozygously deleted in 20% and 10% of the tumours, respectively. No point mutations were found at p16 and p15. The 5' CpG island at the p16 gene was methylated in 20% of the cases. CONCLUSIONS:The tumour suppressor gene p16 is inactivated through homozygous deletion or methylation in a significant proportion of cases of head and neck SCC.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
González MV,Pello MF,López-Larrea C,Suárez C,Menéndez MJ,Coto Edoi
10.1136/jcp.50.6.509subject
Has Abstractpub_date
1997-06-01 00:00:00pages
509-12issue
6eissn
0021-9746issn
1472-4146journal_volume
50pub_type
杂志文章abstract::Tuberous sclerosis is a rare disease with classic primary or secondary changes affecting mainly the cerebrum, skin, kidneys, and heart. Such lesions are generally hamartomatous and thus display malignant features only in rare cases. This paper describes four cases of tuberous sclerosis which were unique in their assoc...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.37.3.272
更新日期:1984-03-01 00:00:00
abstract:AIM:To assess the topographical relation between gastric glands, using the minimum spanning tree (MST), to derive both a model of neighbourhood and quantitative representation of the tissue's architecture, to assess the characteristic features of gastric atrophy, and to assess the grades of gastric atrophy. METHODS:Ha...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.51.12.895
更新日期:1998-12-01 00:00:00
abstract:AIMS:N-terminal pro-B-type natriuretic peptide (NT-proBNP) has been shown to be useful for ruling out heart failure in primary care. In this study, we examined the accuracy of the Cobas h232 point-of-care (POC) instrument in primary care compared with an in-hospital measurement. Furthermore, we investigated the user-fr...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,多中心研究
doi:10.1136/jclinpath-2017-204746
更新日期:2018-06-01 00:00:00
abstract::In a retrospective study of the records of 160 necropsies it was found that, according to the clinical and gross necropsy findings, no clinically important abnormality would have been anticipated in 46% of the tissue samples. The estimated annual cost of processing this material alone was pounds 10,500, including the ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.40.4.459
更新日期:1987-04-01 00:00:00
abstract:AIM:We examined the genetic alterations in a mother and son with multiple eosinophilic chromophobe renal cell carcinomas (chRCCs) showing no other features. METHODS:Germline DNA and bilateral renal cell carcinoma DNA were genetically analysed by whole-exome sequencing. Candidate gene alterations in the first patient's...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2018-205211
更新日期:2018-10-01 00:00:00
abstract:AIMS:The histopathological diagnosis of low-grade dysplasia (LGD) in Barrett's oesophagus (BO) is associated with poor interobserver agreement and guidelines dictate expert review. To facilitate nationwide expert review in the Netherlands, a web-based digital review panel has been set up, which currently consists of ei...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2020-206511
更新日期:2021-01-01 00:00:00
abstract:BACKGROUND:Some uterine endometrioid adenocarcinomas exhibit a distinctive morphological phenotype characterised by the formation of microcystic, elongated and fragmented (MELF) glands. Immunohistochemical studies have suggested that MELF-type changes represent an epithelial-mesenchymal transition which has been associ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2009.069500
更新日期:2010-07-01 00:00:00
abstract::A patient complained of severe diarrhoea for about four years. About 2.5 m of the jejunum was resected and duodenal polyps were also removed at laparotomy. Histologically, they were tubular or tubulovillus adenomas, which closely resembled colonic adenomas. No malignant change was detected in these adenomas. Severe in...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.39.9.981
更新日期:1986-09-01 00:00:00
abstract:PURPOSE:To investigate p53 and hepatoma-derived growth factor (HDGF) expression and their association with clinicopathological features of Ewing family tumour (EFT). EXPERIMENTAL DESIGN:A total of 108 cases of EFT were retrospectively analysed. p53 and HDGF expression were detected using immunohistochemistry, and the ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2013-201705
更新日期:2014-03-01 00:00:00
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journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.36.2.213
更新日期:1983-02-01 00:00:00
abstract::This article reviews recent information concerning the origin of osteoclasts and the local and systemic regulation of their activity. It appears that much of the environmental responsiveness of osteoclasts is mediated by cells of the osteoblastic lineage, which exert a major influence on the localisation, induction, s...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.38.3.241
更新日期:1985-03-01 00:00:00
abstract::The plasma clearance of intravenously administered folic acid was abnormally rapid in all of five patients studied with untreated hyperthyroidism; in three, the basal serum L. casei folate level was subnormal. Four of these patients and another patient were studied at various intervals after partial or complete correc...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.17.6.666
更新日期:1964-11-01 00:00:00
abstract::An imprint culture technique has been employed to study the prevalence and intraoral distribution of Candida albicans in 16 patients with Sjögren's syndrome and in 16 healthy controls matched for age, sex, and dental status. The prevalence and intraoral density of C. albicans was found to be significantly higher at al...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.33.3.282
更新日期:1980-03-01 00:00:00
abstract:AIMS:The WHO classification describes that combined hepatocellular-cholangiocarcinoma, subtypes with stem-cell features, intermediate-cell subtype (CHC-INT) is composed of tumour cells with features intermediate between hepatocytes and cholangiocytes. However, we previously reported that CHC-INT showed a high positive ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2015-203491
更新日期:2016-10-01 00:00:00
abstract::A locally developed, computer-based clinical chemistry laboratory system has been in operation since 1970. This utilises a Digital Equipment Co Ltd PDP 12 and an interconnected PDP 8/F computer. Details are presented of the performance and quality control techniques incorporated into the system. Laboratory performance...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.32.2.100
更新日期:1979-02-01 00:00:00
abstract:AIMS:To assess the effect of cellulosic dialysis membranes on the production of complement degradation products to determine to the role of the classical pathway. METHOD:Complement activation was studied in 33 patients during a single haemodialysis session using cellulosic membranes. Pre- and post-dialysis plasma EDTA...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.47.2.155
更新日期:1994-02-01 00:00:00
abstract:INTRODUCTION/METHODS:After the recognition of contaminating algae on histopathological sections stained by periodic acid Schiff (PAS) and Grocott methods, a detailed audit was undertaken to assess the extent of contamination and its possible source. RESULTS:The contaminating organism was a member of the staurastrum ge...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2005.026187
更新日期:2005-10-01 00:00:00
abstract:AIMS:To evaluate an indirect immunofluorescence flow cytometry technique in a series of patients with large fetomaternal haemorrhage (FMH). METHODS:Patient samples identified by Kleihauer testing in local laboratories as having FMH > 4 ml were sent for flow cytometric analysis. In a proportion of cases the mothers rec...
journal_title:Journal of clinical pathology
pub_type: 临床试验,杂志文章
doi:10.1136/jcp.48.11.1005
更新日期:1995-11-01 00:00:00
abstract::The routine estimation of large numbers of serum folic acid and vitamin B(12) levels using the Baird and Tatlock(1) Analmatic clinical system is described and the method evaluated.Precision, accuracy, and carryover are shown to be at acceptable levels, and the advantages and disadvantages of the use of the system are ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.24.1.18
更新日期:1971-02-01 00:00:00
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.45.5.420
更新日期:1992-05-01 00:00:00
abstract::A male patient with a 17 year history of intractable ulcerative colitis of the entire type was treated by total proctocolectomy. Colonoscopy before surgery did not identify dysplasia. Histological examination of the resected colorectum revealed that, in addition to chronic inflammatory infiltrates, there were 21 areas...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.56.12.963
更新日期:2003-12-01 00:00:00
abstract::Eight isolates of C. albicans were used to determine the frequency with which germ tube formation occurred: on rice extract -Tween 80 agar, on its components, and on 1% bactopeptone agar after three hr at 37 degrees C; in 0.5% aqueous solution of various carbohydrates; in various concentrations of glucose; on 0.5 and ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.28.1.18
更新日期:1975-01-01 00:00:00
abstract:AIMS:PCR amplicon-based next-generation sequencing (NGS) panels are increasingly used for clinical diagnostic assays. Amplification bias is a well-known limitation of PCR amplicon-based approaches. We sought to characterise lower-performance amplicons in an off-the-shelf NGS panel (TruSight Myeloid Sequencing Panel) fo...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2015-203580
更新日期:2016-09-01 00:00:00
abstract:AIMS:Hypomethylating agents (HMAs) exhibit clinical efficacy in patients with myelodysplastic syndromes (MDS) and myelodysplastic/myeloproliferative neoplasms (MDS/MPN). This study was performed to assess residual disease status by flow cytometry immunophenotyping (FCI) methods in patients with MDS or MDS/MPN treated w...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2016-203715
更新日期:2016-04-15 00:00:00
abstract::The sensitivity of methods to detect antibodies to intrinsic factor was assessed. Five sera of known antibody content were tested in 31 laboratories and 30 sera from patients with pernicious anaemia were tested in one laboratory. Five non-commercial methods and two kits for type I antibodies and one non-commercial met...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.42.2.210
更新日期:1989-02-01 00:00:00
abstract::The isotopic estimation of the cortisol secretion rate in man is now an accepted and reliable method of assaying adrenal cortical function.Seven years' experience with its clinical use is reviewed and some practical aspects of technique are considered. The mean normal resting cortisol secretion rate is 16.2 +/- 5.7 mg...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.18.1.82
更新日期:1965-01-01 00:00:00
abstract::Two hundred and forty-seven strains of micro-organisms were isolated from the urinary tract of 121 patients with neurological complaints leading to paraplegia. The commonest infecting organism was E. coli and the commonest type of Proteus was P. mirabilis. Infections with more than one type of organism were present in...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.13.1.54
更新日期:1960-01-01 00:00:00
abstract:BACKGROUND:Kaposi's sarcoma is considered to be an angioproliferative disease associated with a novel herpesvirus (KSHV/HHV8), but the precise pathophysiology of the lesion remains unclear. The study of clonality in Kaposi's sarcoma using X linked DNA polymorphism has been difficult so far, because of a very strong pre...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.50.8.664
更新日期:1997-08-01 00:00:00
abstract::The stability of the commonly used red cell radio labels chromium-51, indium-(111 or 113m), and technetium-99m, within intact red cells and stroma and their distribution within the cell were compared in undamaged and heat damaged red cells in relation to the clinical use of heat damaged cells in the assessment of sple...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.39.7.717
更新日期:1986-07-01 00:00:00
abstract::As patients with multiple myeloma (MM) have a variable clinical course, predictive markers would help determine the appropriate treatment strategy. Clinical staging is commonly used to predict outcome, but tumour marker expression and the underlying genetic changes are increasingly used to assess the biological aggres...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.2007.049585
更新日期:2008-07-01 00:00:00