Abstract：
:Ovarian poly (A) + RNA from Xenopus laevis and Xenopus borealis was used to construct two cDNA libraries which were screened for histone sequences. cDNA clones to H4 mRNA were obtained from both species and an H3 cDNA clone from Xenopus laevis. The complete DNA sequences of these clones have been determined and are presented. These new sequences are compared with other H3 and H4 DNA sequences both in the coding and 3' noncoding regions. We find that there is considerable non-random codon usage in ten H4 genes. In addition there are some sequence similarities in the 3' noncoding regions of H3 and H4 genes.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Turner PC,Woodland HRdoi
10.1093/nar/10.12.3769subject
Has Abstractpub_date
1982-06-25 00:00:00pages
3769-80issue
12eissn
0305-1048issn
1362-4962journal_volume
10pub_type
杂志文章abstract：:A nucleic acid affinity matrix containing a short oligodeoxynucleotide ligand has been prepared as an example of a material which can be used for the rapid and effective isolation of sequence specific DNA binding proteins. Two complementary oligodeoxynucleotides have been employed, one of which contains a small 5'-spa...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.21.10283
更新日期：1988-11-11 00:00:00
abstract：:Bacillus subtilis RecN appears to be an early detector of breaks in double-stranded DNA. In vivo, RecN forms discrete nucleoid-associated structures and in vitro exhibits Mg2+-dependent single-stranded (ss) DNA binding and ssDNA-dependent ATPase activities. In the presence of ATP or ADP, RecN assembles to form large n...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki533
更新日期：2005-04-22 00:00:00
abstract：:G-quadruplexes are dynamic structures folded in G-rich single-stranded DNA regions. These structures have been recognized as a potential nucleic acid based mechanism for regulating multiple cellular processes such as replication, transcription and genomic maintenance. So far, their transcriptional role in vivo during ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw011
更新日期：2016-05-19 00:00:00
abstract：:The SeqA protein binds hemi-methylated GATC sites and forms structures that sequester newly replicated origins and trail the replication forks. Cells that lack SeqA display signs of replication fork disintegration. The broken forks could arise because of over-initiation (the launching of too many forks) or lack of dyn...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx263
更新日期：2017-06-20 00:00:00
abstract：:The genome of Kirsten murine sarcoma virus was formed by recombination between Kirsten murine leukaemia virus sequences, and rat sequences derived from a retrovirus-like '30S' (VL30) genetic element encompassing the Kras oncogene. Using cloned DNAs we have determined the nucleotide sequences of the long terminal repea...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.17.6839
更新日期：1984-09-11 00:00:00
abstract：:DNA assembly forms the cornerstone of modern synthetic biology. Despite the numerous available methods, scarless multi-fragment assembly of large plasmids remains challenging. Furthermore, the upcoming wave in molecular biological automation demands a rethinking of how we perform DNA assembly. To streamline automation...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx132
更新日期：2017-06-20 00:00:00
abstract：:The HIV RT and Protease Sequence Database is an online relational database that catalogs evolutionary and drug-related human immunodeficiency virus (HIV) reverse transcriptase (RT) and protease sequence variation (http://hivdb.stanford.edu). The database contains a compilation of nearly all published HIV RT and protea...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.1.346
更新日期：2000-01-01 00:00:00
abstract：:CS23D (chemical shift to 3D structure) is a web server for rapidly generating accurate 3D protein structures using only assigned nuclear magnetic resonance (NMR) chemical shifts and sequence data as input. Unlike conventional NMR methods, CS23D requires no NOE and/or J-coupling data to perform its calculations. CS23D ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn305
更新日期：2008-07-01 00:00:00
abstract：:RecBCD enzyme acts in the major pathway of homologous recombination of linear DNA in Escherichia coli. The enzyme unwinds DNA and is an ATP-dependent double-strand and single-strand exonuclease and a single-strand endonuclease; it acts at Chi recombination hotspots (5'-GCTGGTGG-3') to produce a recombinogenic single-s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.9.2125
更新日期：1998-05-01 00:00:00
abstract：:In small oocytes of Xenopus species, two sets of 5S RNA genes, oocyte-type and somatic-type, are fully activated. The 5S RNA transcripts are temporarily stored, half in association with TFIIIA to form a 7S particle, the other half in association with tRNA and two proteins (p48 and p43) to form a 42S particle. It has b...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.21.8679
更新日期：1987-11-11 00:00:00
abstract：:Guanine-rich DNA sequences can form G-quadruplexes stabilized by stacked G-G-G-G tetrads in monovalent cation-containing solution. The length and number of individual G-tracts and the length and sequence context of linker residues define the diverse topologies adopted by G-quadruplexes. The review highlights recent so...
journal_title：Nucleic acids research
pub_type： 杂志文章,评审
doi：10.1093/nar/gkm711
更新日期：2007-01-01 00:00:00
abstract：:Understanding the evolutionary and genomic mechanisms responsible for turning the soil-derived saprophytic mycobacteria into lethal intracellular pathogens is a critical step towards the development of strategies for the control of mycobacterial diseases. In this context, Mycobacterium indicus pranii (MIP) is of speci...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks793
更新日期：2012-11-01 00:00:00
abstract：:The 0.048502 megabase (Mb), primarily double-stranded DNA of bacteriophage lambda has single-stranded, complementary termini (cohesive ends) that undergo either spontaneous intramolecular joining to form open circular DNA or spontaneous intermolecular joining to form linear, end-to-end oligomeric DNAs (concatemers); c...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.11.3047
更新日期：1991-06-11 00:00:00
abstract：:Single genes are often subject to alternative splicing, which generates alternative mature mRNAs. This phenomenon is widespread in animals, and observed in over 90% of human genes. Recent data suggest it may also be common in Apicomplexa. These parasites have small genomes, and economy of DNA is evolutionarily favoure...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv311
更新日期：2015-05-19 00:00:00
abstract：:Multiplexed tandem PCR (MT-PCR) is a process for highly multiplexed gene expression profiling. In the first step, multiple primer pairs are added to the RNA to be analysed together with reverse transcriptase and Taq DNA polymerase. Following reverse transcription, the multiplexed amplicons are simultaneously amplified...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gni182
更新日期：2005-11-24 00:00:00
abstract：:Many proteins of the SNF2 family, which share a similar DNA-dependent ATPase/putative helicase domain, are involved in global transcriptional control and processing of DNA damage. We report here the partial cloning and characterization of 89B helicase, a gene encoding a new Drosophila melanogaster member of the SNF2 f...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.16.3121
更新日期：1996-08-15 00:00:00
abstract：:YY1 is ubiquitously expressed zinc finger DNA binding protein. It can act as a transcriptional repressor or activator and, when binding at the initiator element, as a component of the basal transcription complex. Binding sites for YY1 have been reported in a wide variety of promoters and they exhibit substantial diver...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.21.4457
更新日期：1995-11-11 00:00:00
abstract：:We present web servers for analysis of non-coding RNA sequences on the basis of their secondary structures. Software tools for structural multiple sequence alignments, structural pairwise sequence alignments and structural motif findings are available from the integrated web server and the individual stand-alone web s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn222
更新日期：2008-07-01 00:00:00
abstract：:The anaerobically-regulated nirB promoter was used to express heterologous genes in Escherichia coli. Under anaerobic conditions the promoter was able to express tetanus toxin fragment C at approximately 20% total cell protein (tcp) and the Bordetella pertussis antigen pertactin at greater than 30% tcp. These levels a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.11.2889
更新日期：1991-06-11 00:00:00
abstract：:Use of the polymerase chain reaction (PCR) to amplify variable numbers of tandem repeat (VNTR) loci has become widely used in genetic typing. Unfortunately, preferential amplification of small allelic products relative to large allelic products may result in incorrect or ambiguous typing in a heterozygous sample. The ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.15.3050
更新日期：1995-08-11 00:00:00
abstract：:Synthesis and radioiodination of a stannyl oligodeoxyribonucleotide were undertaken to evaluate a gamma ray emitting ODN ligand for thrombus imaging in vivo . Synthesis of the ODN was based on modified automatedbeta-cyanoethyl phosphoramidite chemistry with an organotin nucleoside (dU*) coupled to a thrombin binding a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.14.2897
更新日期：1997-07-15 00:00:00
abstract：:Two new crescent-shaped unsymmetrical cyanine dyes have been synthesised and their interactions with DNA have been investigated by different spectroscopic methods. These dyes are analogues to a minor groove binding unsymmetrical cyanine dye, BEBO, recently reported by us. In this dye, the structure of the known interc...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg821
更新日期：2003-11-01 00:00:00
abstract：:The Ensembl project (https://www.ensembl.org) annotates genomes and disseminates genomic data for vertebrate species. We create detailed and comprehensive annotation of gene structures, regulatory elements and variants, and enable comparative genomics by inferring the evolutionary history of genes and genomes. Our int...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa942
更新日期：2021-01-08 00:00:00
abstract：:Constitutive expression of major histocompatibility complex class II (MHC II) antigens normally occurs in B-lymphocytes and antigen presenting cells of the monocyte/macrophage lineage. However, many malignant tumours and transformed cells express these proteins aberrantly. We demonstrate here that the MHC II DR alpha ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.18.4881
更新日期：1992-09-25 00:00:00
abstract：:The transfer ribonucleic acids (tRNAs) of B. subtilis at different growth phases are examined for changes in the composition and the methylation of minor constituents. The composition of the tRNAs indicates about equal amounts of adenosine and uridine, and of guanosine and cytidine. About 3-4 residues are present as m...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/3.5.1249
更新日期：1976-05-01 00:00:00
abstract：:We have constructed a stable cell line, human embryonal kidney 293M+, containing a lacZ reporter gene controlled by an in vitro methylated hormone-responsive enhancer. Methylation of the enhancer-promoter abolishes lacZ expression controlled by ponasterone A (an analogue of ecdysone). Ponasterone A-induced expression ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/29.17.3603
更新日期：2001-09-01 00:00:00
abstract：:Systematic structure probing experiments (e.g. SHAPE) of RNA mutants such as the mutate-and-map (MaM) protocol give us a direct access into the genetic robustness of ncRNA structures. Comparative studies of homologous sequences provide a distinct, yet complementary, approach to analyze structural and functional proper...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw217
更新日期：2016-06-20 00:00:00
abstract：:We attempted to use the polymerase chain reaction (PCR) to monitor in vitro recombination in a plasmid containing directly repeated sequences. Some of the plasmid preparations which had not been exposed to recombination conditions were however found to behave in the PCR test as if they had undergone homologous recombi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.9.2423
更新日期：1991-05-11 00:00:00
abstract：:We have developed an improved and rapid genomic engineering procedure for the construction of custom-designed microorganisms. This method, which can be performed in 2 days, permits restructuring of the Escherichia coli genome via markerless deletion of selected genomic regions. The deletion process was mediated by a s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn359
更新日期：2008-08-01 00:00:00
abstract：:A pan-genome is the union of the gene sets of all the individuals of a clade or a species and it provides a new dimension of genome complexity with the presence/absence variations (PAVs) of genes among these genomes. With the progress of sequencing technologies, pan-genome study is becoming affordable for eukaryotes w...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw958
更新日期：2017-01-25 00:00:00
