Beta-adrenergic stimulation of Ca2+ fluxes, endocytosis, hexose transport, and amino acid transport in mouse kidney cortex is mediated by polyamine synthesis.

abstract：:Adeno-associated virus (AAV)-mediated gene therapy is currently being pursued as a treatment for the monogenic disorder α-1-antitrypsin (AAT) deficiency. Results from phase I and II studies have shown relatively stable and dose-dependent increases in transgene-derived wild-type AAT after local intramuscular vector adm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Calcedo R,Somanathan S,Qin Q,Betts MR,Rech AJ,Vonderheide RH,Mueller C,Flotte TR,Wilson JM

更新日期：2017-02-14 00:00:00

abstract：:Normal myeloid precursors and MGI(+)D(+) myeloid leukemic cells can be induced to differentiate to mature cells by the normal protein inducer MGI. The sequence of differentiation is the induction of C3 and Fc rosettes, C3 and Fc immune phagocytosis (IP), synthesis and secretion of lysozyme, and formation of mature mac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lotem J,Sachs L

更新日期：1977-12-01 00:00:00

abstract：:In an unprecedented finding, Davis et al. [Davis, R. E., Miller, S., Herrnstadt, C., Ghosh, S. S., Fahy, E., Shinobu, L. A., Galasko, D., Thal, L. J., Beal, M. F., Howell, N. & Parker, W. D., Jr. (1997) Proc. Natl. Acad. Sci. USA 94, 4526-4531] used an unusual DNA isolation method to show that healthy adults harbor a ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hirano M,Shtilbans A,Mayeux R,Davidson MM,DiMauro S,Knowles JA,Schon EA

更新日期：1997-12-23 00:00:00

abstract：:Endothelial cells (ECs) line the mammalian vascular system and respond to the hemodynamic stimulus of fluid shear stress, the frictional force produced by blood flow. When ECs are exposed to shear stress, one of the fastest responses is an increase of K(+) conductance, which suggests that ion channels are involved in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hoger JH,Ilyin VI,Forsyth S,Hoger A

更新日期：2002-05-28 00:00:00

abstract：:Streptococcus pneumoniae is a major human pathogen that successfully adapts to the host environment via an efficient uptake system for free DNA liberated from other organisms in the upper respiratory tract, facilitating immune evasion and drug resistance. Although the initial signaling events leading to pneumococcal c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Balaban M,Bättig P,Muschiol S,Tirier SM,Wartha F,Normark S,Henriques-Normark B

更新日期：2014-02-18 00:00:00

abstract：:Expression of glucokinase in hepatocytes and pancreatic 6-cells is of major physiologic importance to mammalian glucose homeostasis. Liver glucokinase catalyzes the first committed step in the disposal of glucose, and beta-cell glucokinase catalyzes a rate-limiting step required for glucose-regulated insulin release. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Heimberg H,De Vos A,Moens K,Quartier E,Bouwens L,Pipeleers D,Van Schaftingen E,Madsen O,Schuit F

更新日期：1996-07-09 00:00:00

abstract：:A specific high-affinity receptor for leukotriene C4 (LTC4) has been identified on segments of longitudinal smooth muscle from guinea pig ileum, in disrupted cells obtained from the ileal segments, and in subcellular fractions enriched for mitochondrial membranes and plasma membranes, respectively. Specific [3H]LTC4 b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Krilis S,Lewis RA,Corey EJ,Austen KF

更新日期：1984-07-01 00:00:00

abstract：:When an ice crystal is born from liquid water, two key changes occur: (i) The molecules order and (ii) the mobility of the molecules drops as they adopt their lattice positions. Most research on ice nucleation (and crystallization in general) has focused on understanding the former with less attention paid to the latt...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fitzner M,Sosso GC,Cox SJ,Michaelides A

更新日期：2019-02-05 00:00:00

abstract：:A cDNA library derived from human cerebral cortex was screened for the presence of sodium channel alpha subunit-specific clones. Ligation of three overlapping clones generated a full-length cDNA clone, HBA, that provided the complete nucleotide sequence coding for a protein of 2005 amino acids. The predicted structure...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ahmed CM,Ware DH,Lee SC,Patten CD,Ferrer-Montiel AV,Schinder AF,McPherson JD,Wagner-McPherson CB,Wasmuth JJ,Evans GA

更新日期：1992-09-01 00:00:00

abstract：:Whereas NF-kappaB has potent antiapoptotic function in most cell types, it was reported that in pancreatic beta cells it serves a proapoptotic function and may contribute to the pathogenesis of autoimmune type 1 diabetes. To investigate the role of beta cell NF-kappaB in autoimmune diabetes, we produced transgenic mic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim S,Millet I,Kim HS,Kim JY,Han MS,Lee MK,Kim KW,Sherwin RS,Karin M,Lee MS

更新日期：2007-02-06 00:00:00

abstract：:Destruction of mitotic cyclins by ubiquitin-dependent proteolysis is required for cells to complete mitosis and enter interphase of the next cell cycle. In clam eggs, this process is catalyzed by a cyclin-selective ubiquitin carrier protein, E2-C, and the cyclosome/anaphase promoting complex (APC), a 20S particle cont...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Townsley FM,Aristarkhov A,Beck S,Hershko A,Ruderman JV

更新日期：1997-03-18 00:00:00

abstract：:Interleukin-12 (IL-12) is critical for induction of protective immunity against intracellular bacterial infection. However, the mechanisms for efficient induction of IL-12 in innate response remain poorly understood. Here we report that the B type of carbonic anhydrase 6 (Car6-b, which encoded CA-VI B) is essential fo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu J,Xu X,Wang B,Ma Y,Zhang L,Xu H,Hu Y,Wu J,Cao X

更新日期：2017-08-08 00:00:00

abstract：:Relative cerebral glucose metabolism was examined with positron-emission tomography (PET) as a measure of neuronal activation during performance of the classically conditioned eyeblink response in 12 young adult subjects. Each subject received three sessions: (i) a control session with PET scan in which unpaired prese...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Logan CG,Grafton ST

更新日期：1995-08-01 00:00:00

abstract：:Human cells express thousands of different surface proteins that can be used for cell classification, or to distinguish healthy and disease conditions. A method capable of profiling a substantial fraction of the surface proteome simultaneously and inexpensively would enable more accurate and complete classification of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pollock SB,Hu A,Mou Y,Martinko AJ,Julien O,Hornsby M,Ploder L,Adams JJ,Geng H,Müschen M,Sidhu SS,Moffat J,Wells JA

更新日期：2018-03-13 00:00:00

abstract：:The product of the Nijmegen breakage syndrome gene (NBS1) plays crucial roles in DNA damage response through its association with many proteins, including MRE11 and RAD50. However, it remains to be determined exactly how NBS1 accumulates at or near DNA double-strand breaks. Here we report that MDC1 directly binds to N...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu L,Luo K,Lou Z,Chen J

更新日期：2008-08-12 00:00:00

abstract：:Intense proteolysis of cytoskeletal proteins occurs in brain within minutes of transient ischemia, possibly because of the activation of calcium-sensitive proteases (calpains). This proteolytic event precedes overt signs of neuronal degeneration, is most pronounced in regions of selective neuronal vulnerability, and c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee KS,Frank S,Vanderklish P,Arai A,Lynch G

更新日期：1991-08-15 00:00:00

abstract：:Most homeobox genes belonging to the Hox family are sequentially activated in embryonal carcinoma cells upon treatment with retinoic acid. Genes located at the 3' end of each one of the four Hox clusters are activated first, whereas upstream Hox genes are activated progressively later. This activation has been extensi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Faiella A,Zappavigna V,Mavilio F,Boncinelli E

更新日期：1994-06-07 00:00:00

abstract：:The genomic RNA of the avian influenza A virus, fowl plague, was fractionated into eight species by electrophoresis in polyacrylamide-agarose gels containing 6 M urea. The separated 32P-labeled RNA species were characterized by digestion with RNase T1 and fractionation of the resulting oligonucleotides by two-dimensio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McGeoch D,Fellner P,Newton C

更新日期：1976-09-01 00:00:00

abstract：:Engraftment and maintenance of hematopoietic stem and progenitor cells (HSPC) depend on their ability to respond to extracellular signals from the bone marrow microenvironment, but the critical intracellular pathways integrating these signals remain poorly understood. Furthermore, recent studies provide contradictory ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sanchez-Aguilera A,Lee YJ,Lo Celso C,Ferraro F,Brumme K,Mondal S,Kim C,Dorrance A,Luo HR,Scadden DT,Williams DA

更新日期：2011-06-07 00:00:00

abstract：:The activities of cytosolic 3-hydroxy-3-methylglutaryl coenzyme A synthase [3-hydroxy-3-methylglutaryl-CoA acetoacetyl-CoA-lyase (CoA-acylating), EC 4.1.3.5] and microsomal 3-hydroxy-3-methylglutaryl coenzyme A reductase[mevalonate:NADP+ oxidoreductase (CoA-acylating), EC 1.1.1.34], two sequential enzymes in the chole...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Balasubramaniam S,Goldstein JL,Brown MS

更新日期：1977-04-01 00:00:00

abstract：:The management of harmful species, including invasive species, pests, parasites, and diseases, is a major global challenge. Harmful species cause severe damage to ecosystems, biodiversity, agriculture, and human health. In particular, managing harmful species often requires cooperation among multiple agents, such as l...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lampert A

更新日期：2020-05-12 00:00:00

abstract：:Caseinolytic proteases (ClpPs) are large oligomeric protein complexes that contribute to cell homeostasis as well as virulence regulation in bacteria. Although most organisms possess a single ClpP protein, some organisms encode two or more ClpP isoforms. Here, we elucidated the crystal structures of ClpP1 and ClpP2 fr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zeiler E,List A,Alte F,Gersch M,Wachtel R,Poreba M,Drag M,Groll M,Sieber SA

更新日期：2013-07-09 00:00:00

abstract：:Prompted by the increasing interest in networks in many fields, we present an attempt at unifying points of view and analyses of these objects coming from the social sciences, statistics, probability and physics communities. We apply our approach to the Newman-Girvan modularity, widely used for "community" detection, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bickel PJ,Chen A

更新日期：2009-12-15 00:00:00

abstract：:A near-full-length casbene synthase cDNA clone, pCS7, was isolated by using a partial cDNA clone, pCS4, to probe a lambda gt10 library constructed from poly(A)+ RNA from elicited castor bean seedlings. The cDNA insert had a length of 1983 bases with a polyadenylate tail of 19 bases. Translation of the cDNA sequence re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mau CJ,West CA

更新日期：1994-08-30 00:00:00

abstract：:Enhanced transductional recombination between specialized transducing phage lambda plac5 and plasmid F42lac depends on cis- and trans-acting factors. By constructing a series of recombinant molecules, the cis-acting site required for enhanced recombination has been identified as oriT, the origin of conjugational trans...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Seifert HS,Porter RD

更新日期：1984-12-01 00:00:00

abstract：:Climate change over the next century is predicted to cause widespread maladaptation in natural systems. This prediction, as well as many sustainable management and conservation practices, assumes that species are adapted to their current climate. However, this assumption is rarely tested. Using a large-scale common ga...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Browne L,Wright JW,Fitz-Gibbon S,Gugger PF,Sork VL

更新日期：2019-12-10 00:00:00

abstract：:Urinary acidification by the turtle bladder is due to a H+-ATPase that is located in the luminal membrane. The rate of H+ transport is stimulated by an increase in the ambient CO2. Using the fluorescent dye acridine orange, we showed that the mitochondria-rich cell of this equilibrium contains vesicles whose internal ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gluck S,Cannon C,Al-Awqati Q

更新日期：1982-07-01 00:00:00

abstract：:One of the strategies used by organisms to adapt to life under conditions of short energy supply is to use the by-product pyrophosphate to support cation gradients in membranes. Transport reactions are catalyzed by membrane-integral pyrophosphatases (PPases), which are classified into two homologous subfamilies: H(+)-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Luoto HH,Baykov AA,Lahti R,Malinen AM

更新日期：2013-01-22 00:00:00

abstract：:Polyaromatic hydrocarbons are ubiquitous environmental chemicals that are important mutagens and carcinogens. The purpose of this study was to determine whether genes within the major histocompatibility complex (MHC) influence their biological activities. Cell-mediated immunity to dimethylbenz(a)anthracene (DMBA) was ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Elmets CA,Athar M,Tubesing KA,Rothaupt D,Xu H,Mukhtar H

更新日期：1998-12-08 00:00:00

abstract：:Thebaine, an intermediate of morphine biosynthesis in the poppy plant, Papaver somniferum, was transformed to oripavine, codeine, and morphine by rat liver, kidney, and brain microsomes in the presence of an NADPH-generating system. The formation of morphine, codeine, and oripavine was identified by a specific RIA, HP...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kodaira H,Spector S

更新日期：1988-02-01 00:00:00