Abstract:
:Interleukin-12 (IL-12) is critical for induction of protective immunity against intracellular bacterial infection. However, the mechanisms for efficient induction of IL-12 in innate response remain poorly understood. Here we report that the B type of carbonic anhydrase 6 (Car6-b, which encoded CA-VI B) is essential for host defense against Listeria monocytogenes (LM) infection by epigenetically promoting IL-12 expression independent of its carbonic anhydrase activity. Deficiency of Car6-b attenuated IL-12 production upon LM infection both in vitro and in vivo. Car6-/- mice were more susceptible to LM infection with less production of IL-12. Mechanistically, the nuclear localized CA-VI B selectively promotes IL-12 expression by interaction with protein arginine N-methyltransferase 5 (PRMT5), which reduces symmetric dimethylation of histone H3 arginine 8 modification (H3R8me2s) at Il12 promoters to facilitate chromatin accessibility, selectively enhancing c-Rel binding to the Il12b promoter. Our findings add insights to the epigenetic regulation of IL-12 induction in innate immunity.
journal_name
Proc Natl Acad Sci U S Aauthors
Xu J,Xu X,Wang B,Ma Y,Zhang L,Xu H,Hu Y,Wu J,Cao Xdoi
10.1073/pnas.1700917114subject
Has Abstractpub_date
2017-08-08 00:00:00pages
8620-8625issue
32eissn
0027-8424issn
1091-6490pii
1700917114journal_volume
114pub_type
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